β-glucanase and xylanase for beef cattle on tropical pasture

Authors

  • Matheus Leonardi Damasceno Universidade Estadual do Oeste do Paraná https://orcid.org/0000-0002-2086-5373
  • Mariana Barbizan Universidade Estadual do Oeste do Paraná
  • Eriton Egídio Lisboa Valente Universidade Estadual do Oeste do Paraná https://orcid.org/0000-0001-6551-1790
  • Silvana Teixeira Carvalho Universidade Estadual do Oeste do Paraná https://orcid.org/0000-0003-4044-299X
  • Kachire Zoz Universidade Federal de Viçosa
  • Eduardo Eustáquio Mesquita Universidade Estadual do Oeste do Paraná https://orcid.org/0000-0003-4009-362X
  • Sidnei Antônio Lopes Universidade Estadual de Maringá
  • Victor Valério Carvalho DSM Nutritional Products

DOI:

https://doi.org/10.5433/1679-0359.2024v45n6p1909

Keywords:

Digestibility, Ruminants, Supplementation.

Abstract

The aim of this study was to assess the impact of administering an energy-protein supplement with fibrolytic enzymes, either singly or in a blend, on the intake, digestibility, ruminal, and metabolic parameters in grazing beef cattle. Five rumen-cannulated Nellore steers, averaging 36 months of age and a body weight of 559.57 ± 35 kg were evaluated using a 5 x 5 Latin Square design. The treatments included a protein-energy supplement (2 g/kg BW) without additives (Control), or supplemented with 4 g β-glucanase/animal (BGLU); 4 g xylanase/animal (XYLA); 4 g β-glucanase and 1 g xylanase/animal (BGLU+XYLA); and 4 g xylanase and 1 g β-glucanase/animal (XYLA+BGLU). The administration of either single fibrolytic enzymes or the enzyme blend did not significantly influence (P > 0.05) the intakes of forage dry matter (DM), total DM, crude protein (CP), neutral detergent fiber (NDF), organic matter (OM), digestible OM, or the digestibility coefficients of DM, NDF, CP, and OM. Similarly, the use of these enzymes individually or combined did not impact (P > 0.05) the levels of rumen pH, volatile fatty acids, ruminal ammonia nitrogen, microbial nitrogen, serum urea nitrogen, or urinary nitrogen excretion. Providing fibrolytic enzymes, individually or in blends, does not modify the nutrient intake, digestibility, or metabolism in beef cattle on tropical pastures receiving low levels of protein-energy supplements.

Downloads

Author Biographies

Matheus Leonardi Damasceno, Universidade Estadual do Oeste do Paraná

Student at the Graduate Program in Animal Science, Universidade Estadual do Oeste do Paraná, UNIOESTE, Marechal Cândido Rondon, PR, Brazil.

Mariana Barbizan, Universidade Estadual do Oeste do Paraná

Student at the Graduate Program in Animal Science, Universidade Estadual do Oeste do Paraná, UNIOESTE, Marechal Cândido Rondon, PR, Brazil.

Eriton Egídio Lisboa Valente, Universidade Estadual do Oeste do Paraná

Prof., Department in Graduate Program in Animal Science, UNIOESTE, Marechal Cândido Rondon, PR, Brazil.

Silvana Teixeira Carvalho, Universidade Estadual do Oeste do Paraná

Profa., Department in Graduate Program in Animal Science, UNIOESTE, Marechal Cândido Rondon, PR, Brazil.

Kachire Zoz, Universidade Federal de Viçosa

Prof., Department of Animal Science, Universidade Federal de Viçosa, UFV, MG, Brazil.

Eduardo Eustáquio Mesquita, Universidade Estadual do Oeste do Paraná

Prof., Department in Graduate Program in Animal Science, UNIOESTE, Marechal Cândido Rondon, PR, Brazil.

Sidnei Antônio Lopes, Universidade Estadual de Maringá

Student at the Graduate Program in Animal Science, Universidade Estadual de Maringá, UEM, Maringá, PR, Brazil.

Victor Valério Carvalho, DSM Nutritional Products

Researcher, Innovation and Applied Science Department, DSM Nutritional Products, São Paulo, SP, Brazil.

References

Adesogan, A. T., Ma, Z. X., Romero, J. J., Arriola, K. G., Jiang, Y., Oyebade, A., Paula, E. M., Pech-Cervantes, A. A., Romero, J. J., Ferraretto, L. F., Vyas, D., Oliveira, A. S., Ma, Z. X., Lean, I. J., Giurcanu, M. C., Adesogan, A. T. (2017). Ruminant Nutrition Symposium: Improving cell wall digestion and animal performance with fibrolytic enzymes. Journal of Dairy Science, 100, 4513-4527. doi: 10.3168/jds.2018-15334 DOI: https://doi.org/10.3168/jds.2018-15334

Association of Official Analytical Chemists AOAC. (1990). Official methods of analysis. (15a. ed.). Washington.

Arriola, K. G., Oliveira, A. S., Ma, Z. X., Lean, I. J., Giurcanu, M. C., & Adesogan, A. T. (2017). A meta-analysis on the effect of dietary application of exogenous fibrolytic enzymes on the performance of dairy cows. Journal of Dairy Science, 100(6), 4513-4527. doi: 10.3168/jds.2016-12103 DOI: https://doi.org/10.3168/jds.2016-12103

Azevedo, A. C. C. G., Costa, K. A. D. P., Collao-Saenz, E. A., Dias, F. J. D. S., Severiano, E. D. C., & Cruvinel, W. S. (2014). Valor nutricional das silagens de capins Xaraes e Piatã emurchecidas ou acrescidas de aditivos. Acta Scientiarum. Animal Sciences, 36, 25-31. doi: 10.4025/actascianimsci.v36i1.18993 DOI: https://doi.org/10.4025/actascianimsci.v36i1.18993

Beauchemin, K. A., Ribeiro, G. O., Ran, T., Milani, M. R. M., Yang, W., Khanaki, H., & McAllister, T. A. (2019). Recombinant fibrolytic feed enzymes and ammonia fibre expansion (AFEX) pretreatment of crop residues to improve fibre degradability in cattle. Animal Feed Science and Technology, 256, 114260. doi: 10.1016/j.anifeedsci.2019.114260 DOI: https://doi.org/10.1016/j.anifeedsci.2019.114260

Bobade, H., Gupta, A., & Sharma, S. (2022). Beta-glucan. In H. Bohade, A. Gupta & S. Sharma (Eds.), Nutraceuticals and health care (pp. 343-358). Academic Press. DOI: https://doi.org/10.1016/B978-0-323-89779-2.00013-2

Cevallos, J. H. A., Muñoz, S. S. G., Rodríguez, J. M. P., Hernández-Garay, A., Montañez-Valdez, O. D., & Ayala-Oseguera, J. (2007). Enzimas fibrolíticas exógenas en la digestibilidad in vitro de cinco ecotipos de Brachiaria. Agronomía Mesoamericana, 18(1), 11-17. doi: 10.15517/am.v18i1.5032 DOI: https://doi.org/10.15517/am.v18i1.5032

Chaney, A. L., & Marbach, E. P. (1962). Modified reagents for determination of urea and ammonia. Clinical Chemistry, 8(2), 130-132. doi: 10.1093/clinchem/8.2.130 DOI: https://doi.org/10.1093/clinchem/8.2.130

Cherdthong, A., Seankamsorn, A., Suriyapha, C., Chanjula, P., Wanapat, M., 2018. Effect of beta-glucan supplementation on feed intake, digestibility of nutrients and ruminal fermentation in Thai native beef cattle. Journal of Animal Physiology and Animal Nutrition, 102, 1509-1514. doi: 10.1111/jpn.12989 DOI: https://doi.org/10.1111/jpn.12989

Colombatto, D., Mould, F. L., Bhat, M. K., Morgavi, D. P., Beauchemin, K. A., & Owen, E. (2003). Influence of fibrolytic enzymes on the hydrolysis and fermentation of pure cellulose and xylan by mixed ruminal microorganisms in vitro. Journal of Animal Science, 81(4), 1040-1050. doi: 10.2527/2003.8141040x DOI: https://doi.org/10.2527/2003.8141040x

Erwin, E. S., Marco, G. J., & Emery, E. M. (1961). Volatile fatty acids in rumen fluid of dairy cattle as influenced by ration and stage of lactation. Journal of Dairy Science, 44(8), 1162-1166. https://doi.org/10.3168/jds.S0022-0302(61)89598-7Felix, E. P., & Cardoso, A. A. (2004). Amônia (NH3) atmosférica: fontes, transformação, sorvedouros e métodos de análise. Química Nova, 27(1), 123-130. doi: 10.1590/S0100-40422004000100022 DOI: https://doi.org/10.1590/S0100-40422004000100022

Freiria, L. B. da, Zervoudakis, J. T., Paula, N. F. de, Silva Cabral, L. da, Tedeschi, L. O., Rosa, P. I. J. L. da, Melo, A. C. B., & Possamai, A. J. (2018). Do fibrolytic, proteolytic and amylolytic enzymes influence the in vitro fermentation characteristics of forage? Semina: Ciências Agrárias, 39(3), 1143-1153. doi: 10.5433/1679-0359.2018v39n3p1143 DOI: https://doi.org/10.5433/1679-0359.2018v39n3p1143

Gandra, J. R., Miranda, G. A., Goes, R. H. T. B., Takiya, C. S., Del Valle, T. A., Oliveira, E. R., Freitas, J. E. J., Araqui, H. M. C., & Santos, A. L. A. V. (2017). Fibrolytic enzyme supplementation through ruminal bolus on eating behavior, nutrient digestibility and ruminal fermentation in Jersey heifers fed either corn silage-or sugarcane silage-based diets. Animal Feed Science and Technology, 231(5), 29-37. doi: 10.1016/j.anifeedsci.2017.06.009 DOI: https://doi.org/10.1016/j.anifeedsci.2017.06.009

George, S. K., Dipu, M. T., Mehra, U. R., Singh, P., Verma, A. K., & Ramgaokar, J. S. (2006). Improved HPLC method for the simultaneous determination of allantoin, uric acid and creatinine in cattle urine. Journal of Chromatography B, 832(1), 134-137. doi: 10.1016/j.jchromb.2005.10.051 DOI: https://doi.org/10.1016/j.jchromb.2005.10.051

Greenwood P. L., Paull D. R., McNally J., Kalinowski T., Ebert D., Little B., Smith D. V., Rahman A., Valencia P., Ingham A. B., Bishop-Hurley G. J. (2017) Use of sensor-determined behaviours to develop algorithms for pasture intake by individual grazing cattle. Crop and Pasture Science, 68, 1091-1099. doi: 10.1071/CP16383 DOI: https://doi.org/10.1071/CP16383

Hristov, A. N., McAllister, T. A., & Cheng, K. J. (2000). Intraruminal supplementation with increasing levels of exogenous polysaccharide-degrading enzymes: effects on nutrient digestion in cattle fed a barley grain diet. Journal of Animal Science, 78(2), 477-487. doi: 10.2527/2000.782477x DOI: https://doi.org/10.2527/2000.782477x

Kondratovich, L. B., Sarturi, J. O., Hoffmann, C. A., Ballou, M. A., Trojan, S. J., & Campanili, P. R. (2019). Effects of dietary exogenous fibrolytic enzymes on ruminal fermentation characteristics of beef steers fed high-and low-quality growing diets. Journal of Animal Science, 97(7), 3089-3102. doi: 10.1093/jas/skz165 DOI: https://doi.org/10.1093/jas/skz165

McAllister, T. A., Hristov, A. N., Beauchemin, K. A., Rode, L. M., & Cheng, K. J. (2001). Enzymes in ruminant diets. In M. R. Bedford & G. G. Parvez (Eds.), Enzymes in Farm Animal Nutrition (pp. 273-298). CABI Publishing. doi: 10.1079/9780851993873.0273 DOI: https://doi.org/10.1079/9780851993935.0273

Mendoza, G. D., Loera-Corral, O., Plata-Pérez, F. X., Hernández-García, P. A., & Ramírez-Mella, M. (2014). Considerations on the use of exogenous fibrolytic enzymes to improve forage utilization. The Scientific World Journal, 2014, 1-8. doi: 10.1155/2014/247437

Mertens, D. R., Allen, M., Carmany, J., Clegg, J., Davidowicz, A., Drouches, M., Frank, K., Gambin, D., Garkie, M., Gildemeister, B., Jeffress, D., Jeon, C. S., Jones, D., Kaplan, D., Kim, G. N., Kobata, S., Main, D., Moua, X., Paul, B., Robertson, J., Taysom, D., Thiex, N., Williams, J., & Wolf, M. (2002). Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: Collaborative study. Journal of AOAC International, 85(6), 1217-1240. doi: 10.1093/jaoac/85.6.1217

Pinos-Rodríguez, J. M., González, S. S., Mendoza, G. D., Bárcena, R., Cobos, M. A., Hernández, A., & Ortega, M. E. (2002). Effect of exogenous fibrolytic enzyme on ruminal fermentation and digestibility of alfalfa and rye-grass hay fed to lambs. Journal of Animal Science, 80(11), 3016-3020. doi: 10.2527/2002.801 13016x DOI: https://doi.org/10.2527/2002.80113016x

Poppi, D. P., Quigley, S. P., Silva, T. A. C. C. D., & McLennan, S. R. (2018). Challenges of beef cattle production from tropical pastures. Revista Brasileira de Zootecnia, 47, Article e20160419. doi: 10.1590/rbz4720160419 DOI: https://doi.org/10.1590/rbz4720160419

Rabee, A. E., Sayed Alahl, A. A., Lamara, M., & Ishaq, S. L. (2022). Fibrolytic rumen bacteria of camel and sheep and their applications in the bioconversion of barley straw to soluble sugars for biofuel production. Plos One, 17(1), e0262304. doi: 10.1371/journal.pone.0262304 DOI: https://doi.org/10.1371/journal.pone.0262304

Ran, T., Saleem, A. M., Shen, Y., Ribeiro, G. O., Jr., Beauchemin, K. A., Tsang, A., Yang, W., & McAllister, T. A. (2019). Effects of a recombinant fibrolytic enzyme on fiber digestion, ruminal fermentation, nitrogen balance, and total tract digestibility of heifers fed a high forage diet. Journal of Animal Science, 97(8), 3578-3587. doi: 10.1093/jas/skz216 DOI: https://doi.org/10.1093/jas/skz216

Silva, L. F. C., Valadares, S. D. C., Fº., Chizzotti, M. L., Rotta, P. P., Prados, L. F., Valadares, R. F. D., Prados, L. F, Valadares, R. F. D., Zanetti, D., & Braga, J. M. D. S. (2012). Creatinine excretion and relationship with body weight of Nellore cattle. Revista Brasileira de Zootecnia, 41(3), 807-810. doi: 10.1590/S1516-35982012000300046 DOI: https://doi.org/10.1590/S1516-35982012000300046

Soltan, Y. A., Abdalla, A. L., Silva, L. R., Natel, A. S., Morsy, A. S., & Louvandini, H. (2013). Response of different tropical pasture grass species to treatments with fibrolytic enzymes in terms of in vitro ruminal nutrient degradation and methanogenesis. Animal Nutrition and Feed Technology, 13(3), 551-568. doi: 10.1155/2014/247437 DOI: https://doi.org/10.1155/2014/247437

Song, S. D., Chen, G. J., Guo, C. H., Rao, K. Q., Gao, Y. H., Peng, Z. L., Zhang, Z. F., Bai, X., Wang, B. X., Chen, Z. H., Fu, X. S., & Zhu, W. L. (2018). Effects of exogenous fibrolytic enzyme supplementation to diets with different NFC/NDF ratios on the growth performance, nutrient digestibility and ruminal fermentation in Chinese domesticated black goats. Animal Feed Science and Technology, 236(1), 170-177. doi: 10.1016/j.anifeedsci.2017.12.008 DOI: https://doi.org/10.1016/j.anifeedsci.2017.12.008

Titgemeyer, E. C., Armendariz, C. K., Bindel, D. J., Greenwood, R. H., & Löest, C. A. (2001). Evaluation of titanium dioxide as a digestibility marker for cattle. Journal of Animal Science, 79(4), 1059-1063. doi: 10.2527/2001.7941059x DOI: https://doi.org/10.2527/2001.7941059x

Valente, T. N. P., Detmann, E., Queiroz, A. C. D., Valadares, S. D. C., Fº., Gomes, D. I., & Figueiras, J. F. (2011). Evaluation of ruminal degradation profiles of forages using bags made from different textiles. Revista Brasileira de Zootecnia, 40(11), 2565-2573. doi: 10.1590/S1516-35982011001100039 DOI: https://doi.org/10.1590/S1516-35982011001100039

Van Soest, P. J. (1994). Nutritional ecology of the ruminant (Vol. 476). Cornell University Press. DOI: https://doi.org/10.7591/9781501732355

Downloads

Published

2024-11-28

How to Cite

Damasceno, M. L., Barbizan, M., Valente, E. E. L., Carvalho, S. T., Zoz, K., Eustáquio Mesquita, E., … Carvalho, V. V. (2024). β-glucanase and xylanase for beef cattle on tropical pasture. Semina: Ciências Agrárias, 45(6), 1909–1920. https://doi.org/10.5433/1679-0359.2024v45n6p1909

Issue

Section

Articles

Most read articles by the same author(s)

1 2 3 > >> 

Similar Articles

1 2 > >> 

You may also start an advanced similarity search for this article.