Efeitos do nível de proteína na ingestão, digestibilidade, e gasto energético em novilhas leiteiras
DOI:
https://doi.org/10.5433/1679-0359.2020v41n6Supl2p3211Palavras-chave:
Bovinos, Consumo, Excreção de nitrogênio, Urea.Resumo
O balanceamento das dietas proteicas afeta tanto a eficiência no uso de proteínas quanto a eficiência no uso de energia em bovinos. Este estudo teve como objetivo avaliar os efeitos dos níveis de proteína na dieta sobre consumo, digestibilidade e gasto de energia em novilhas Holandesas. Quatro novilhas Holandesas com um peso corporal (PC) médio de 266,5 ± 10,7 kg foram distribuídas em delineamento quadrado latino 4 x 4. Cada período era composto de 14 d para adaptação e 6 d para amostragens. As novilhas foram alimentadas com 9,12,15 e 18% de proteína bruta (PB). Fezes e urina totais foram coletadas. O líquido ruminal foi coletado às 2, 4, 6 e 8 h após a alimentação. O gasto de energia foi avaliado às 6h, 12h, 18h e 12h por uma técnica de máscara de circuito fechado. O consumo de matéria seca (MS), matéria orgânica (MO) e fibra em detergente neutro (FDN) foram similares entre os tratamentos (P > 0,05). Embora a digestibilidade do FDN não tenha sido alterada (P > 0,05), a digestibilidade de MS, MO, PB, carboidratos não-fibrosos e nutrientes digestíveis totais aumentou linearmente (P < 0,05) com o aumento dos níveis de PB na dieta. A excreção de uréia urinária e o nitrogênio retido aumentaram linearmente (P < 0,01). Os níveis de PB na dieta não afetaram (P = 0,53) o gasto de energia em novilhas. A concentração de nitrogênio amoniacal atingiu o pico em 2-4 h após a alimentação. O aumento dos níveis dietéticos de PB de 9 para 18% não afeta o consumo de ração e o gasto de energia em novilhas. Entretanto, melhora a digestibilidade da MO e a retenção de nitrogênio.Métricas
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Referências
Association of Official Analytical Chemists (1990). Official methods of analysis of AOAC International (15nd ed.). Washington, DC: AOAC.
Belanche, A., Doreau, M., Edwards, J. E., Moorb, J. M., Pinloche, E., & Newbold, C. J. (2012). Shifts in the rumen microbiota due to the type of carbohydrate and level of protein ingested by dairy cattle are associated with changes in rumen fermentation. Journal of Nutrition, 142(9), 1684-1692. doi: 10.3945/ jn.112.159574
Calsamiglia, S., Ferret, A., Reynolds, C. K., Kristensen, N. B., & Van Vuuren, A. M. (2010). Strategies for optimizing nitrogen use by ruminants. Journal of Animal Bioscience, 4(7), 1184-1196. doi: 10.1017/S1 751731110000911
Chaney, A. L., & Marbach, E. P. (1962). Modified reagents for determination of urea and ammonia. Clinical chemistry, 8(2), 130-132. Retrieved from http: webpages.icav.up.pt/ptdc/CVT/09848/2008/Chaney,% 20011962.pdf.
Detmann, E., Paulino, M. F., Valadares, S. C., Fo, & Lana, R. P. (2007). Fatores controladores de consumo em suplementos múltiplos fornecidos ad libitum para bovinos manejados a pasto. Cadernos Técnicos de Veterinária e Zootecnia, 55, 73-93
Detmann, E., Valente, É. E. L., Batista, E. D., & Huhtanen, P. (2014). An evaluation of the performance and efficiency of nitrogen utilization in cattle fed tropical grass pastures with supplementation. Livestock Science, 162, 141-153. doi: 10.1016/j.livsci.2014.01.029
Dijkstra, J., Reynolds, C. K., Kebreab, E., Bannink, A., Ellis, J. L., France, J., & Van Vuuren, A. M. (2013). Challenges in ruminant nutrition: towards minimal nitrogen losses in cattle. In Energy and protein metabolism and nutrition in sustainable animal production (pp. 47-58). Wageningen Academic Publishers, Wageningen. Retrieved fromhttp://old.eaap.org/docs/members/9789086867813EAAP134-e. pdf
Dong, L. F., Zhang, W. B., Zhang, N. F., Tu, Y., & Diao, Q. Y. (2017). Feeding different dietary protein to energy ratios to Holstein heifers: effects on growth performance, blood metabolites and rumen fermentation parameters. Journal of Animal Physiology and Animal Nutrition, 101(1), 30-37. doi: 10. 1111/jpn.12493
Hristov, A. N., Ropp, J. K., Grandeen, K. L., Abedi, S., Etter, R. P., Melgar, A., & Foley, A. K. (2005). Effect of carbohydrate source on ammonia utilization in lactating dairy cows. Journal of Animal Science, 83(2), 408-421. doi: 10.2527/2005.8324
Illius, A. W., & Jessop, N. S. (1996). Metabolic constraints on voluntary intake in ruminants. Journal of Animal Science, 74(12), 3052-3062. doi: 10.2527/1996.74123052x
Koenig, K. M., & Beauchemin, K. A. (2013). Nitrogen metabolism and route of excretion in beef feedlot cattle fed barley-based finishing diets varying in protein concentration and rumen degradability. Jornal of Animal Science, 91(5), 2310-2320. doi: 10.2527/jas.2012-5653
Lazzarini, I., Detmann, E., Sampaio, C. B., Paulino, M. F.,Valadares, S. C., Fº., Souza, M. A., & Oliveira, F. B. (2009). Intake and digestibility in cattle fed low-quality tropical forage and supplemented with nitrogenous compounds. Revista Brasileira de Zootecnia, 38(10), 2021-2030. doi: 10.1590/S1516-3598 2009001000024
Leng, R. A. (1990). Factors affecting the utilization of “poor-quality” forages by ruminants particularly under tropical conditions. Nutrition Research Reviews, 3(1), 277-303. doi: 10.1079/NRR19900016
Licitra, G., Hernandez, T. M., & Van Soest, P. J. (1996). Standardization of procedures for nitrogen fractionation of ruminant feeds. Animal Feed Science and Technology, 57(4), 347-358. doi: 10.1016/03 77-8401(95)00837-3
McLean, J. A. (1972). On the calculation of heat production from open-circuit calorimetric measurements. Bristish Journal of Nutrition, 27(3), 597-600. doi: 10.1079/BJN19720130
Mertens, D. R. (2002). Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: collaborative study. Journal of AOAC International, 85(6), 1217-1240.
Mutsvangwa, T., Davies, K. L., McKinnon, J. J., & Christensen, D. A. (2016). Effects of dietary crude protein and rumen-degradable protein concentrations on urea recycling, nitrogen balance, omasal nutrient flow, and milk production in dairy cows. Journal of Dairy Science, 99(8), 6298-6310. doi: 10. 3168/jds.2016-10917
Nelson, D. L., Lehninger, A. L., & Cox, M. M. (2008). Lehninger principles of biochemistry. Macmillan.
National Research Council (2001). Requirements of dairy cattle (7nd ed.). Washington, D.C: Nutrient Requirements of Dairy Cattle. Natl. Acad. Press.
Reis, W. L. S., Detmann, E., Batista, E. D., Rufino, L. M. A., Gomes, D. I., Bento, C. B. P., & Valadares, S. C., Fº. (2016). Effects of ruminal and post-ruminal protein supplementation in cattle fed tropical forages on insoluble fiber degradation, activity of fibrolytic enzymes, and the ruminal microbial community profile. Animal Feed Science and Technology, 218(23), 1-16. doi: 10.1016/j.anifeedsci.2016.05.001
Reynolds, C. K. ( 2006). Splanchnic metabolism of amino acids. In K. Sejrsen, T. Hvelplund, & M. O. Nielsen (Eds.), Ruminant physiology. Digestion, metabolism and impact of nutrition on gene expression, immunology and stress. Wageningen Academic.
Reynolds, C. K., Crompton, L. A., & Mills, J. A. N. (2011). Improving the efficiency of energy utilisation in cattle. Animal. Production Science, 51(1), 6-12. doi: 10.1071/AN10160
Richardson, D., Felgate, H., Watmough, N., Thomson, A., & Baggs, E. (2009). Mitigating release of the potent greenhouse gas N2O from the nitrogen cycle - could enzymic regulation hold the key? Trends in Biotectechnology, 27(7), 388-397. doi: 10.1016/j.tibtech.2009.03.009
Sampaio, C. B., Detmann, E., Lazzarini, I., Souza, M. A., Paulino, M. F., & Valadares, S. C., Fº. (2009). Rumen dynamics of neutral detergent fiber in cattle fed low-quality tropical forage and supplemented with nitrogenous compounds. Revista Brasileira de Zootecnia, 38(3), 560-569. doi: 10.1590/S1516-35 982009000300023
Sampaio, C. B., Detmann, E., Paulino, M. F., Valadares, S. C., Fº., Souza, M. A., Lazzarini, I., & Queiroz, A. C. (2010). Intake and digestibility in cattle fed low-quality tropical forage and supplemented with nitrogenous compounds. Tropical Animal Health and Production, 42(10), 1471-1479. doi: 10.1007/s11 250-010-9581-7
Schroeder, G. F., & Titgemeyer, E. C. (2008). Interaction between protein and energy supply on protein utilization in growing cattle: a review. Livestock Science, 114(1), 1-10. doi: 10.1016/j.livsci.2007.12.0 08
Souza, M. A., Detmann, E., Paulino, M. F., Sampaio, C. B., Lazzarini, Í., & Valadares, S. C., Fº. (2010). Intake, digestibility and rumen dynamics of neutral detergent fibre in cattle fed low-quality tropical forage and supplemented with nitrogen and/or starch. Tropical Animal Health and Production, 42(6), 1299-1310. doi: 10.1007/s11250-010-9566-6
SAS Institute Inc. (2017). Cary, NC; University Edition.
Steinberg-Yfrach, G., Rigaud, J. L., Durantini, E. N., Moore, A. L., Gust, D., & Moore, T. A. (1998). Light-driven production of ATP catalysed by F0F1-ATP synthase in an artificial photosynthetic membrane. Nature, 392 (6675,), 479-482. doi: 10.1038/33116
Susenbeth, A., Mayer, R., Koehler, B., & Neumann, O. (1998). Energy requirement for eating in cattle. Journal Animal Science, 76(10), 2701-2705. doi: 10.2527/199876102701x
Belanche, A., Doreau, M., Edwards, J. E., Moorb, J. M., Pinloche, E., & Newbold, C. J. (2012). Shifts in the rumen microbiota due to the type of carbohydrate and level of protein ingested by dairy cattle are associated with changes in rumen fermentation. Journal of Nutrition, 142(9), 1684-1692. doi: 10.3945/ jn.112.159574
Calsamiglia, S., Ferret, A., Reynolds, C. K., Kristensen, N. B., & Van Vuuren, A. M. (2010). Strategies for optimizing nitrogen use by ruminants. Journal of Animal Bioscience, 4(7), 1184-1196. doi: 10.1017/S1 751731110000911
Chaney, A. L., & Marbach, E. P. (1962). Modified reagents for determination of urea and ammonia. Clinical chemistry, 8(2), 130-132. Retrieved from http: webpages.icav.up.pt/ptdc/CVT/09848/2008/Chaney,% 20011962.pdf.
Detmann, E., Paulino, M. F., Valadares, S. C., Fo, & Lana, R. P. (2007). Fatores controladores de consumo em suplementos múltiplos fornecidos ad libitum para bovinos manejados a pasto. Cadernos Técnicos de Veterinária e Zootecnia, 55, 73-93
Detmann, E., Valente, É. E. L., Batista, E. D., & Huhtanen, P. (2014). An evaluation of the performance and efficiency of nitrogen utilization in cattle fed tropical grass pastures with supplementation. Livestock Science, 162, 141-153. doi: 10.1016/j.livsci.2014.01.029
Dijkstra, J., Reynolds, C. K., Kebreab, E., Bannink, A., Ellis, J. L., France, J., & Van Vuuren, A. M. (2013). Challenges in ruminant nutrition: towards minimal nitrogen losses in cattle. In Energy and protein metabolism and nutrition in sustainable animal production (pp. 47-58). Wageningen Academic Publishers, Wageningen. Retrieved fromhttp://old.eaap.org/docs/members/9789086867813EAAP134-e. pdf
Dong, L. F., Zhang, W. B., Zhang, N. F., Tu, Y., & Diao, Q. Y. (2017). Feeding different dietary protein to energy ratios to Holstein heifers: effects on growth performance, blood metabolites and rumen fermentation parameters. Journal of Animal Physiology and Animal Nutrition, 101(1), 30-37. doi: 10. 1111/jpn.12493
Hristov, A. N., Ropp, J. K., Grandeen, K. L., Abedi, S., Etter, R. P., Melgar, A., & Foley, A. K. (2005). Effect of carbohydrate source on ammonia utilization in lactating dairy cows. Journal of Animal Science, 83(2), 408-421. doi: 10.2527/2005.8324
Illius, A. W., & Jessop, N. S. (1996). Metabolic constraints on voluntary intake in ruminants. Journal of Animal Science, 74(12), 3052-3062. doi: 10.2527/1996.74123052x
Koenig, K. M., & Beauchemin, K. A. (2013). Nitrogen metabolism and route of excretion in beef feedlot cattle fed barley-based finishing diets varying in protein concentration and rumen degradability. Jornal of Animal Science, 91(5), 2310-2320. doi: 10.2527/jas.2012-5653
Lazzarini, I., Detmann, E., Sampaio, C. B., Paulino, M. F.,Valadares, S. C., Fº., Souza, M. A., & Oliveira, F. B. (2009). Intake and digestibility in cattle fed low-quality tropical forage and supplemented with nitrogenous compounds. Revista Brasileira de Zootecnia, 38(10), 2021-2030. doi: 10.1590/S1516-3598 2009001000024
Leng, R. A. (1990). Factors affecting the utilization of “poor-quality” forages by ruminants particularly under tropical conditions. Nutrition Research Reviews, 3(1), 277-303. doi: 10.1079/NRR19900016
Licitra, G., Hernandez, T. M., & Van Soest, P. J. (1996). Standardization of procedures for nitrogen fractionation of ruminant feeds. Animal Feed Science and Technology, 57(4), 347-358. doi: 10.1016/03 77-8401(95)00837-3
McLean, J. A. (1972). On the calculation of heat production from open-circuit calorimetric measurements. Bristish Journal of Nutrition, 27(3), 597-600. doi: 10.1079/BJN19720130
Mertens, D. R. (2002). Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: collaborative study. Journal of AOAC International, 85(6), 1217-1240.
Mutsvangwa, T., Davies, K. L., McKinnon, J. J., & Christensen, D. A. (2016). Effects of dietary crude protein and rumen-degradable protein concentrations on urea recycling, nitrogen balance, omasal nutrient flow, and milk production in dairy cows. Journal of Dairy Science, 99(8), 6298-6310. doi: 10. 3168/jds.2016-10917
Nelson, D. L., Lehninger, A. L., & Cox, M. M. (2008). Lehninger principles of biochemistry. Macmillan.
National Research Council (2001). Requirements of dairy cattle (7nd ed.). Washington, D.C: Nutrient Requirements of Dairy Cattle. Natl. Acad. Press.
Reis, W. L. S., Detmann, E., Batista, E. D., Rufino, L. M. A., Gomes, D. I., Bento, C. B. P., & Valadares, S. C., Fº. (2016). Effects of ruminal and post-ruminal protein supplementation in cattle fed tropical forages on insoluble fiber degradation, activity of fibrolytic enzymes, and the ruminal microbial community profile. Animal Feed Science and Technology, 218(23), 1-16. doi: 10.1016/j.anifeedsci.2016.05.001
Reynolds, C. K. ( 2006). Splanchnic metabolism of amino acids. In K. Sejrsen, T. Hvelplund, & M. O. Nielsen (Eds.), Ruminant physiology. Digestion, metabolism and impact of nutrition on gene expression, immunology and stress. Wageningen Academic.
Reynolds, C. K., Crompton, L. A., & Mills, J. A. N. (2011). Improving the efficiency of energy utilisation in cattle. Animal. Production Science, 51(1), 6-12. doi: 10.1071/AN10160
Richardson, D., Felgate, H., Watmough, N., Thomson, A., & Baggs, E. (2009). Mitigating release of the potent greenhouse gas N2O from the nitrogen cycle - could enzymic regulation hold the key? Trends in Biotectechnology, 27(7), 388-397. doi: 10.1016/j.tibtech.2009.03.009
Sampaio, C. B., Detmann, E., Lazzarini, I., Souza, M. A., Paulino, M. F., & Valadares, S. C., Fº. (2009). Rumen dynamics of neutral detergent fiber in cattle fed low-quality tropical forage and supplemented with nitrogenous compounds. Revista Brasileira de Zootecnia, 38(3), 560-569. doi: 10.1590/S1516-35 982009000300023
Sampaio, C. B., Detmann, E., Paulino, M. F., Valadares, S. C., Fº., Souza, M. A., Lazzarini, I., & Queiroz, A. C. (2010). Intake and digestibility in cattle fed low-quality tropical forage and supplemented with nitrogenous compounds. Tropical Animal Health and Production, 42(10), 1471-1479. doi: 10.1007/s11 250-010-9581-7
Schroeder, G. F., & Titgemeyer, E. C. (2008). Interaction between protein and energy supply on protein utilization in growing cattle: a review. Livestock Science, 114(1), 1-10. doi: 10.1016/j.livsci.2007.12.0 08
Souza, M. A., Detmann, E., Paulino, M. F., Sampaio, C. B., Lazzarini, Í., & Valadares, S. C., Fº. (2010). Intake, digestibility and rumen dynamics of neutral detergent fibre in cattle fed low-quality tropical forage and supplemented with nitrogen and/or starch. Tropical Animal Health and Production, 42(6), 1299-1310. doi: 10.1007/s11250-010-9566-6
SAS Institute Inc. (2017). Cary, NC; University Edition.
Steinberg-Yfrach, G., Rigaud, J. L., Durantini, E. N., Moore, A. L., Gust, D., & Moore, T. A. (1998). Light-driven production of ATP catalysed by F0F1-ATP synthase in an artificial photosynthetic membrane. Nature, 392 (6675,), 479-482. doi: 10.1038/33116
Susenbeth, A., Mayer, R., Koehler, B., & Neumann, O. (1998). Energy requirement for eating in cattle. Journal Animal Science, 76(10), 2701-2705. doi: 10.2527/199876102701x
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2020-11-06
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Araújo, L. C., Valente, E. E. L., Filipini, V. da T., Carvalho, S. T., Stahlhofer, M., Cabral, C. H. A., Damasceno, M. L., & Barbizan, M. (2020). Efeitos do nível de proteína na ingestão, digestibilidade, e gasto energético em novilhas leiteiras. Semina: Ciências Agrárias, 41(6Supl2), 3211–3224. https://doi.org/10.5433/1679-0359.2020v41n6Supl2p3211
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