Prevalence and risk factors for Leptospira spp. in dairy cattle in western Paraná, Brazil
DOI:
https://doi.org/10.5433/1679-0359.2023v44n3p1097Keywords:
Seroprevalence, . Protease., Leptospirosis, Cattle, Maintenance host.Abstract
Leptospirosis is caused by spirochete bacteria of the genus Leptospira and is considered the most widespread zoonosis worldwide. It is an important agent that causes animal production to decrease. In cattle, it affects especially the reproductive tract. The objective of this study was to determine the seroprevalence of Leptospira spp., molecularly detect the bacteria in tissues of aborted fetuses, and identify the main risk factors associated with infection in cattle in dairy farms in Western Paraná. For this purpose, 600 bovine serum samples from 60 properties and 17 bovine fetuses from nine properties were collected. Data about the properties were also collected through an epidemiological questionnaire to assess the main risk factors associated with Leptospira spp. infection. The serum samples were analyzed using microscopic agglutination test (MAT), and the fetal tissues using nested polymerase chain reaction (nested PCR). Seroprevalence of Leptospira spp. in dairy cattle in Western Paraná was 39.83% (239/600) and none of the analyzed fetuses were positive for Leptospira spp. The main risk factors identified are related to the production system, reproductive management, and the presence of dogs on the property. Leptospira spp. infection is widely spread in the cattle population in Western Paraná.
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References
Adler, B., & Moctezuma, A. P. (2010). Leptospira and leptospirosis. Veterinary Microbiology, 140(3-4), 287-96. doi: 10.1016/j.vetmic.2009.03.012 DOI: https://doi.org/10.1016/j.vetmic.2009.03.012
Balamurugan, V., Alamuri, A., Bharathkumar, K., Patil, S. S., Govindaraj, G. N., Nagalingam, M., Krishnamoorthy, P., Rahman, H., & Shome, B. R. (2018). Prevalence of Lesptospira serogroup-specific antibodies in cattle associated with reproductive problems in endemic states of India. Tropical Animal Health and Production, 50(1), 1131-1138. doi: 10.1007/s11250-018-1540-8 DOI: https://doi.org/10.1007/s11250-018-1540-8
Barnabé, N. N. C., Soares, R. R., Barros, D. K. S., Nogueira, D. B., Costa, F. T. R., Araújo, J. P., Jr., Malossi, C. D., Ullmann, L. S., Costa, D. F., Silva, M. L. C. R., Higino, S. S. S., Santos, C. S. A. B., Azevedo, S. S., & Alves, C. J. (2023). Bovine Leptospirosis in caatinga biome, Brazil: new insights into diagnosis and epidemiology. Tropical Medicine Infectious Disease, 8(3), 177. doi: 10.3390/tropicalmed8030177 DOI: https://doi.org/10.3390/tropicalmed8030177
Chiebao, D. P., Valadas, S. Y., Minervino, A. H., Castro, V., Romaldini, A. H., Calhau, A.S. & Soares, R. M. (2015). Variables Associated with Infections of Cattle by Brucella abortus, Leptospira spp. and Neospora spp. in Amazon Region in Brazil. Transboundary Emerging Diseases, 62(5), 30-36. doi: 10.1111/tbed.12201 DOI: https://doi.org/10.1111/tbed.12201
Chiriboga, J., Barragan, V., Arroyo, G., Sosa, A., Birdsell, D. N., España, K. & Trueba, G. (2015). High prevalence of intermediate Leptospira spp. DNA in febrile humans from urban and rural Ecuador. Emerging Infectious Diseases, 21(12), 2141-2147. doi: 10.3201/eid2112.140659 DOI: https://doi.org/10.3201/eid2112.140659
Cortez, A., Castro, A. M. G., Heinemann, M. B., Soares, R. M., Leite, R. C., Scarcelli, E., Genovez, M. E., Alfieri, A. A., & Richtzenhain, L. J. (2006). Detecção de ácidos nucléicos de Brucella spp., Leptospira spp., herpesvirus bovino e vírus da diarreia viral bovina, em fetos bovinos abortados e em animais mortos no perinatal. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 58(6), 1226-1228. doi: 10.1590/S0102-09352006000600036 DOI: https://doi.org/10.1590/S0102-09352006000600036
Desvars, A., Michault, A., & Bourhy, P. (2013). Leptospirosis in the western Indian Ocean islands: what is known so far? Veterinary Research, 44(1), 80. doi: 10.1186/1297-9716-44-80 DOI: https://doi.org/10.1186/1297-9716-44-80
Ellis, W. A. (1994). Leptospirosis as a cause of reproductive failure. Veterinary Clinics of North America: Food Animal Practice, 10(3), 463-478. doi: 10.1016/s0749-0720(15)30532-6 DOI: https://doi.org/10.1016/S0749-0720(15)30532-6
Ellis, W. A. (2015). Animal leptospirosis. In B. Adler (Ed.), Leptospira and Leptospirosis. Current topics in microbiology and immunology (pp. 99-137). Berlin, Heidelberg: Springer. DOI: https://doi.org/10.1007/978-3-662-45059-8_6
Fávero, J. F., Araújo, H. L. de, Lilenbaum, W., Machado, G., Tonin, A. A., Baldissera, M. D., Stefani, L. M., & Silva, A. S. da. (2017). Bovine leptospirosis: Prevalence, associated risk factors for infection and their cause-effect relation. Microbial Pathogenesis, 107(1), 149-154. doi: 10.1016/j.micpath.2017.03.032 DOI: https://doi.org/10.1016/j.micpath.2017.03.032
Fávero, J. F., Pinheiro, S. R., Vasconcellos, S. A., Morais, Z. M., Ferreira, F., & Ferreira, J. S., Neto. (2001). Leptospirose Bovina - variantes sorológicas predominantes em colheitas efetuadas no período de 1984 a 1997 em rebanhos de 21 estados do Brasil. Arquivos do Instituto Biológico, 68(2), 29-35. https://repositorio.usp.br/item/001246498
Gagnon, C. A., Allam, O., Drolet, R., & Tremblay, D. (2010). Quebec: detection of bovine lymphotropic herpesvirus DNA in tissues of a bovine aborted fetus. Canadian Veterinary Journal, 51(9), 1021-1022. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2920160/pdf/cvj_09_1021.pdf
Genovez, M. E., Scarcelli, E., Rojas, S., Giorg, W., & Kaneto, C. N. (1993). Isolamentos bacterianos de fetos abortados bovinos examinados no Instituto Biológico de São Paulo, no período de 1985 a 1992. Brazilian Journal of Veterinary Research and Animal Science, 30(2), 107-112. doi: 10.11606/issn.1678-4456.bjvras.1993.52021 DOI: https://doi.org/10.11606/issn.1678-4456.bjvras.1993.52021
Gonçalves, D. D., Pastre, G. B., Rey, L. M. R., Fazoli, K. G. Z., Silva, L. L. D., Ferreira, L. R. P., Fritzen, J. T. T., Chideroli, R. T., Durel, L., Decuadro-Hansen, G., Lourenço, E. L. B., Piau, R., Jr., Barbosa, L. N., Pereira, U. P., & Santos, I. C. D. (2021). Leptospira spp. in naturally infected dairy cow from a Brazilian border region. Vector Borne Zoonotic Diseases, 21(11), 864-869. doi: 10.1089/vbz.2021.0040 DOI: https://doi.org/10.1089/vbz.2021.0040
Hashimoto, V. Y., Chideroli, R. T., Ribeiro, J., Alfieri, A. A., Costa, G. M. da, Pereira, U. P., Freitas, J. C. de. (2017). Serological and molecular findings in diagnosis of leptospirosis serovar Hardjo in a dairy bovine herd. Semina: Ciências Agrárias, 38(5), 3155-3164. doi: 10.5433/1679-0359.2017v38n5p3155 DOI: https://doi.org/10.5433/1679-0359.2017v38n5p3155
Hashimoto, V. Y., Dias, J. A., Spohr, K. A. H., Silva, M. C. P., Andrade, M. G. B., Muller, E. E., & Freitas, J. C. (2012). Prevalência e fatores de risco associados à Leptospira spp. em rebanhos bovinos da região centro-sul do estado do Paraná. Pesquisa Veterinária Brasileira, 32(2), 99-105. doi: 10.1590/S0100-736X2012000200001 DOI: https://doi.org/10.1590/S0100-736X2012000200001
Instituto Brasileiro de Geografia e Estatística (2016). Banco de dados agregados. http://www.sidra.ibge.gov. br/
Lage, A. P., Leite, R. M. H., Thompson, J. A., Bandeira, D. A., Herrmann, G. P., Moreira, E. C., & Gonçalves, V. S. P. (2007). Serology for Leptospira sp. in cattle of the State of Paraíba, Brazil. Arquivos do Instituto Biológico, 74(3), 185-190. doi: 10.1590/1808-1657v74p1852007 DOI: https://doi.org/10.1590/1808-1657v74p1852007
Langoni, H., Souza, L. C. de, Silva, A. V. da, Luvizotto, M. C., Paes, A. C., & Lucheis, S. B. (1999). Incidence of leptospiral abortion in Brazilian dairy cattle. Preventive Veterinary Medicine, 40(3-4), 271-275. doi: 10.1016/s0167-5877(99)00020-3 DOI: https://doi.org/10.1016/S0167-5877(99)00020-3
Lehmann, J. S., Matthias, M. A., Vinetz, J. M., & Fouts, D. E. (2014). Leptospiral pathogenomics. Pathogens (Basel, Switzerland), 3(2), 280-308. doi: 10.3390/pathogens3020280 DOI: https://doi.org/10.3390/pathogens3020280
Mérien, F., Amouriaux, P., Perolat, P., Baranton, G., & Saint Girons, I. (1992). Polymerase chain reaction for detection of Leptospira spp. in clinical samples. Journal of Clinical Microbiology, 30(9), 2219-2224. doi: 10.1128/jcm.30.9.2219-2224.1992 DOI: https://doi.org/10.1128/jcm.30.9.2219-2224.1992
Miashiro, A. F., Vasconcellos, S. A., Morais, Z. M., Souza, G. O., Leal, J. M., Fº., Figueiredo A. O., & Pellegrin, A. O. (2018). Prevalência de leptospirose em rebanhos bovinos no Pantanal de Mato Grosso do Sul. Pesquisa Veterinária Brasileira, 38(1), 41-47. doi: 10.1590/1678-5150-PVB-4992. DOI: https://doi.org/10.1590/1678-5150-pvb-4992
Mughini-Gras, L., Bonfanti, L., Natale, A., Comin, A., Ferronato, A., La Greca, E., Patregnani, T., Lucchese, L., & Marangon, S. (2014). Application of an integrated outbreak management plan for the control of leptospirosis in dairy cattle herds. Epidemiology and Infection, 142(6), 1172-1181. doi: 10.1017/S0950268813001817 DOI: https://doi.org/10.1017/S0950268813001817
Paes, A. C. (2016). Leptospirose canina. In Jane Megid, Marcio Garcia Ribeiro e Antonio Carlos Paes, Doenças infecciosas em animais de produção e de companhia (Cap. 34, pp. 356-377). São Paulo.
Paixão, A. P., Santos, H. P., Coêlho, L. M., Moraes, A. H., Carvalho, R. F. B., Costa, V. M., Fº., Oliveira, E. A. A., Soares, D. M., & Beserra, P. A. (2016). Leptospira spp. em bovinos leiteiros do estado do Maranhão, Brasil: frequência, fatores de risco e mapeamento de rebanhos reagentes. Arquivos do Instituto Biológico, 83(1), 1-12. doi: 10.1590/1808-1657001022014 DOI: https://doi.org/10.1590/1808-1657001022014
Pasqualotto, W., Sehnem, S., & Winck, C. A. (2015). Incidência de rinotraqueíte infecciosa bovina (IBR), diarreia viral bovina (BVD) e Leptospirose em bovinos leiteiros da região Oeste de Santa Catarina - Brasil. Revista em Agronegócio e Meio Ambiente, Maringá (PR), 2(8), 249-270. doi: 10.17765/2176-9168.2015v8n2p249-270 DOI: https://doi.org/10.17765/2176-9168.2015v8n2p249-270
Peregrine, A. S., Martin, S. W., Hopwood, D. A., Duffield, T. F., McEwen, B., Hobson, J. C., & Hietala, S. K. (2006). Neospora caninum and Leptospira serovar serostatus in dairy cattle in Ontario. Canadian Veterinary Journal, 47(5), 467-470. doi: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1444906/pdf/cvj47pg467.pdf
Picardeau, M. (2013). Diagnosis and epidemiology of leptospirosis. Médecine et Maladies Infectieuses, 43(1), 1-9. doi: 10.1016/j.medmal.2012.11.005 DOI: https://doi.org/10.1016/j.medmal.2012.11.005
Pinto, P. S., Libonati, H., & Lilenbaum, W. (2017). A systematic review of leptospirosis on dogs, pigs, and horses in Latin America. Tropical Animal Health and Production, 49(2), 231-238. doi: 10.1007/s11250-016-1201-8 DOI: https://doi.org/10.1007/s11250-016-1201-8
Porto, Y. F., Pinto, A., Neto, Bernardi, F., Possa, M. G., Mota, M. F., Martinez, A. C., Merlini, L. S., & Berber, R. C. (2018). Occurrence of brucellosis, leptospirosis and neosporosis in cows with retained placenta in Southwest Paraná, Brazil. Pesquisa Veterinária Brasileira, 38(8), 1537-1542. doi: 10.1590/1678-5150-PVB-5415 DOI: https://doi.org/10.1590/1678-5150-pvb-5415
Sarmento, A. M. C., Azevedo, S. S., Morais, Z. M., Souza, G. O., Oliveira, F. C. S., Gonçales, A. P., Miraglia, F., & Vasconcellos, S. A. (2012). Emprego de estirpes Leptospira spp. isoladas no Brasil na microtécnica de soroaglutinação microscópica aplicada ao diagnóstico da leptospirose em rebanhos bovinos de oito estados brasileiros. Pesquisa Veterinária Brasileira, 32(7), 601-606. doi: 10.1590/S0100-736X2012000700003 DOI: https://doi.org/10.1590/S0100-736X2012000700003
Silva, F. J., Conceição, W. L. F., Fagliari, J. J., Girio, R. J. S., Dias, R. A., Borba, M. R. B., & Mathias, L. A. (2012). Prevalence and risk factors of bovine leptospirosis in the State of Maranhão, Brazil. Pesquisa Veterinária Brasileira, 32(4), 303-312. doi: 10.1590/S0100-736X2012000400006 DOI: https://doi.org/10.1590/S0100-736X2012000400006
Snak, A., Garcia, F. G., Lara, A. A., Pena, H. F. J., & Osaki, S. C. (2018). Neospora caninum in properties in the west region of Paraná, Brazil: prevalence and risk factors. Revista Brasileira de Parasitologia Veterinária, 27(1), 51-59. doi: 10.1590/S1984-29612018001 DOI: https://doi.org/10.1590/s1984-29612018001
Suepaul, S. M., Carrington, C. V., Campbell, M., Borde, G., & Adesiyun, A. A. (2011). Seroepidemiology of leptospirosis in livestock in Trinidad. Tropical Animal Health and Production, 43(2), 367-375. doi: 10.1007/s11250-010-9698-8 DOI: https://doi.org/10.1007/s11250-010-9698-8
Thrusfield, M. (2007). Veterinary epidemiology (3a ed.). Butterworths.
Torres-Castro, M. A., Cruz-Camargo, B. E., Medina-Pinto, R., Moguel-Lehmer, C., Arcila-Fuentes, W., Medina, R., Ortiz-Esquivel, J., López-Ávila, A., Noh-Pech, H. R., Panti-May, J. A., Rodríguez-Vivas, R. I., & Puerto, F. I. (2017). Absence of molecular evidence of Leptospira spp. in urine samples collected from rodents captured in Yucatán, México. Austral Journal of Veterinary Sciences, 49(3), 195-198. doi: 10.4067/S0719-81322017000300195 DOI: https://doi.org/10.4067/S0719-81322017000300195
World Organization for Animal Health (2021). Manual of diagnostic tests and vaccines for terrestrial animals, Leptospirosis. https://www.woah.org/fileadmin/Home/eng/Health_standards/tahm/3.01.12_LEPTO.pdf
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