Use of different combinations of enzyme complexes in broiler diets
DOI:
https://doi.org/10.5433/1679-0359.2021v42n5p2907Keywords:
Additives, Carbohydrases, Performance, Phytase. Nutrition, . Protease.Abstract
Two experiments were carried out to investigate the effect of “on top” addition of different enzyme complexes, the enzyme alfa-galactosidase and three sources of the enzyme phytase available on the market, in broiler diets. In the first experiment, 1260 one-day-old Cobb 500R chicks were distributed into seven treatments in a completely randomized design (CRD) with six replicates and 30 birds/replicate. Treatments consisted of combinations of different enzyme complexes, namely, complex A (phytase, protease, xylanase, beta-glucanase, cellulase, amylase, pectinase), complex B (protease and cellulase) and complex C (xylanase, amylase and protease); isolated alfa-galactosidase (GAL); and three sources of phytase (P1, P2 and P3) in the diet. The treatments were formulated as follows: T1 - basal diet (BD); T2 - BD + enzyme complex A + enzyme complex B (BDAB); T3 - BDAB + GAL; T4 - BD + complex A + GAL; T5 - BD + complex C + P1 + GAL (BDCG); T6 - BDCG + P2; and T7 - BDCG + P3. The following variables were measured in the experimental period of 42 days: feed intake (FI), weight gain (WG), average final weight (AFW), feed conversion (FC), and carcass yield. Significant differences occurred for AFW, WG and FC in the pre-starter phase. In the second experiment, 112 Cobb 500R chicks aged 25 days were distributed into seven treatments in a CRD with four replicates and four birds/replicate. Treatments were the same as in the first experiment. Nutrient digestibility was evaluated in an experimental period of seven days. Differences were found in the metabolism coefficient of ether extract (MCEE). Dietary inclusion of enzyme complexes improves the AFW and WG of chickens from 1 to 7 days of age and MCEE in the grower phase.Downloads
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References
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Kaczmarek, S. A., Rogiewicz, A., Mogielnicka, M., Rutkowski, A., Jones, R. O., & Slominski, B. A. (2014). The effect of protease, amylase, and nonstarch polysaccharide-degrading enzyme supplementation on nutrient utilization and growth performance of broiler chickens fed corn-soybean meal-based diets. Poultry Science, 93(7), 1745-1753. doi: 10.3382/ps.2013-03739
Keyser, K., Kuterna, L., Kaczmarek, S., Rutkowski, A., & Vanderbeke, E. (2016). High dosing NSP enzymes for total protein and digestible amino acid reformulation in a wheat/corn/soybean meal diet in broilers. Journal of Applied Poultry Research, 25(2), 239-246. doi: 10.3382/japr/pfw006
Law, F. L., Zulkifli, I., Soleimani, A. F., Liang, J. B., & Awad, E. A. (2018). The effects of low-protein diets and protease supplementation on broiler chickens in a hot and humid tropical environment. Asian-Australasian Journal of Animal Science, 31(8), 1291-1300. doi: 10.5713/ajas.17.0581
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Matterson, L. D., Potter, L. M., Stutz, M. W., & Singsen, E. P. (1965). The metabolizable energy of feeds ingredients for chickens. Agricultural Experiment Station Research Report, (7), 1-11.
Miranda, L. M. B., Goulart, C. C., Leite, S. C. B., Batista, A. S. M., & Lima, R. C. (2017). Farelo de algodão em dietas com ou sem suplementação de enzimas para frangos de corte. Revista Ciência Agronômica, 48(4), 690-699. doi: 10.5935/1806-6690.20170080
Moura, F. A. S., Dourado, L. R. B., Farias, L. A., Lopes, J. B., Lima, S. B. P., & Fernandes, M. L. (2019). Complexos enzimáticos sobre a energia metabolizável e digestibilidade dos nutrientes do milheto para frangos de corte. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 71(3), 990-996. doi: 10.1590/ 1678-4162-10021
Munyaka, P. M., Nandha, N. K., Kiarie, E., Nyachoti, C. M., & Khafipour, E. (2016). Impact of combined β-glucanase and xylanase enzymes on growth performance, nutrients utilization and gut microbiota in broiler chickens fed corn or wheat-based diets. Poultry Science, 95(3), 528-540. doi: 10.3382/ps/pev333
Noy, Y., & Sklan, D. (2002). Nutrient use in chicks during the first week posthatch. Poultry Science, 81(3), 391-399. doi: 10.1093/ps/81.3.391
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Sakomura, N. K., & Rostagno, H. S. (2016). Métodos de pesquisa em nutrição de monogástricos. São Paulo: Editora FUNEP.
Shirmohammad, F., & Mehri, M. (2011). Effects of dietary supplementation of multi-enzyme complex on the energy utilization in rooster and performance of broiler chicks. African Journal of Biotechnology, 10(38), 7541-7547. doi: 10.5897/AJB10.2260
Silva, D. J., & Queiroz, A. C. (2002). Análise de alimentos: métodos químicos e biológicos. Viçosa, MG: Editora da Universidade Federal de Viçosa.
Tang, D., Hao, S., Liu, G., Nian, F., & Ru, Y. (2014). Effects of maize source and complex enzymes on performance and nutrient utilization of broilers. Asian-Australasian Journal of Animal Science, 27(12), 1755-1762. doi: 10.5713/ajas.2014.14255
Walk, C. L., Juntunen, K., Paloheimo, M., & Ledoux, D. R. (2019). Evaluation of novel protease enzymes on growth performance and nutriente digestibility of poultry: enzyme dose response. Poultry Science, 98(11), 5525-5532. doi: 10.3382/ps/pez299
Yuan, L., Wang, M., Zhang, X., & Wang, Z. (2017). Effects of protease and non-starch polysaccharide enzyme on performance, digestive function, activity and gene expression of endogenous enzyme of broilers. Plos One, 12(3), 1-13. doi: 10.1371/journal.pone.0173941
Zou, J., Zheng, P., Zhang, K., Ding, X., & Bai, S. (2013). Effects of exogenous enzymes and dietary energy on performance and digestive physiology of broilers. Journal of Animal Science and Biotechnology, 4(14), 1-9. doi: 10.1186/2049-1891-4-14
Alabi, O. O., Shoyombo, A. J., Akpor, O. B., Oluba, O. M., & Adeyonu, A. G. (2019). Exogenous enzymes and the digestibility of nutrients by broilers: a mini review. International Journal of Poultry Science, 18(9), 404-409. doi: 10.3923/ijps.2019.404.409
Alagawany, M., Attia, A. I., Ibrahim, Z. A., Mahmoud, R. A., & El-Sayed, S. A. (2017). The effectiveness of dietary sunflower meal and exogenous enzyme on growth, digestive enzymes, carcass traits, and blood chemistry of broilers. Environmental Science and Pollution Research International, 24(13), 12319-12327. doi: 10.1007/s11356-017-8934-4
Amerah, A. M., Romero, L. F., Awati, A., & Ravindran, V. (2017). Effect of exogenous xylanase, amylase, and protease as single or combined activities on nutrient digestibility and growth performance of broilers fed corn/soy diets. Poultry Science, 96(4), 807-816. doi: 10.3382/ps/pew297
Apperson, K. D., & Cherian, G. (2017). Effect of whole flax seed and carbohydrase enzymes on gastrointestinal morphology, muscle fatty acids, and production performance in broiler chickens. Poultry Science, 96(5), 1228-1234. doi: 10.3382/ps/pew371
Barbosa, J. A., Fº., Oliveira, J. P. F., Boas, A. D. C. V., Almeida, M., Dornellas, T., Hoffmann, A. C.,… Oba, A. (2018). Características produtivas e qualitativas de frangos de corte alimentados com diferentes complexos enzimáticos. Boletim de Indústria Animal, 75, 1-9. doi: 10.17523/bia.2018.v75.e1427
Batal, A. B., & Parsons, C. M. (2002). Effects of age on nutrient digestibility in chicks fed different diets. Poultry Science, 81(3), 400-407. doi: 10.1093/ps/81.3.400
Café, M. B., Borges, C. A., Fritts, C. A., & Waldroup, P. W. (2002). Avizyme improves performance of broilers fed corn-soybean meal-based diets. Journal of Applied Poultry Research, 11(1), 29-33. doi: 10. 1093/japr/11.1.29
Carvalho, D. P., Leandro, N. S. M., Andrade, M. A., Oliveira, H. F., Pires, M. F., Teixeira, K. A.,… Stringhini, J. H. (2020). Protease inclusion in plant- and animal-based broiler diets: performance, digestibility and biometry of digestive organs. South African Journal of Animal Science, 50(2), 291-301. doi: 10.4314/sajas.v50i2.12
Cowieson, A. J., Lu, H., Ajuwon, K. M., Knap, I., & Adeola, O. (2016). Interactive effects of dietary protein source and exogenous protease on growth performance, immune competence and jejunal health of broiler chickens. Animal Production Science, 57(2), 252-261. doi: 10.1071/AN15523
Dalólio, F. S., Moreira, J., Vaz, D. P., Albino, L. F. T., Valadares, L. R., Pires, A. V., & Pinheiro, S. R. F. (2016). Exogenous enzymes in diets for broilers. Revista Brasileira de Saúde e Produção Animal, 17(2), 149-161. doi: 10.1590/S1519-99402016000200003
Dosković, V., Bogosavljević-Bosković, S., Pavlovski, Z., Milošević, B., Škrbić, Z., Rakonjac, S., & Petričević, V. (2013). Enzymes in broiler diets with special reference to protease. World’s Poultry Science Journal, 69(2), 343-360. doi: 10.1017/S0043933913000342
Fernandes, J. I. M., Bortoluzzi, C., Burin, A. M., Jr., Rorig, A., Perini, R., & Cristo, A. B. (2015). Effect of different enzymatic supplements in diets of broilers raised at high stocking density. Journal of Veterinary Medicine and Research, 2(1), 1-4. doi: 10.47739/veterinarymedicine.1016
Ipcak, H. H., Cardozo, P. W., Denli, M., & Escobero, S. (2019). Effect of dietary multi-enzyme supplementation on growth performance and nutrient digestibility of broilers fed mash or pellet diets. Proceedings of the International Animal Science Conference, Cappadocia, Turkey, 11.
Kaczmarek, S. A., Rogiewicz, A., Mogielnicka, M., Rutkowski, A., Jones, R. O., & Slominski, B. A. (2014). The effect of protease, amylase, and nonstarch polysaccharide-degrading enzyme supplementation on nutrient utilization and growth performance of broiler chickens fed corn-soybean meal-based diets. Poultry Science, 93(7), 1745-1753. doi: 10.3382/ps.2013-03739
Keyser, K., Kuterna, L., Kaczmarek, S., Rutkowski, A., & Vanderbeke, E. (2016). High dosing NSP enzymes for total protein and digestible amino acid reformulation in a wheat/corn/soybean meal diet in broilers. Journal of Applied Poultry Research, 25(2), 239-246. doi: 10.3382/japr/pfw006
Law, F. L., Zulkifli, I., Soleimani, A. F., Liang, J. B., & Awad, E. A. (2018). The effects of low-protein diets and protease supplementation on broiler chickens in a hot and humid tropical environment. Asian-Australasian Journal of Animal Science, 31(8), 1291-1300. doi: 10.5713/ajas.17.0581
Leite, P. R. S. C., Leandro, N. S. M., Stringhini, J. H., Café, M. B., Gomes, N. A., & Jardim, R. M., Fº. (2011). Desempenho de frangos de corte e digestibilidade de rações com sorgo ou milheto e complexo enzimático. Pesquisa Agropecuária Brasileira, 46(3), 280-286. doi: 10.1590/S0100-204X20110003000 08
Llamas-Moya, S., Girdler, C. P., Shalash, S. M. M., Atta, A. M., Gharib, H. B., Morsy, E. A.,… Elmenawey, M. (2019). Effect of a multicarbohydrase containing α-galactosidase enzyme on the performance, carcass yield, and humoral immunity of broilers fed corn-soybean meal-based diets of varying energy density. Journal of Applied Poultry Research, 29(1), 142-151. doi: 10.1016/j.japr.2019.10.001
Matterson, L. D., Potter, L. M., Stutz, M. W., & Singsen, E. P. (1965). The metabolizable energy of feeds ingredients for chickens. Agricultural Experiment Station Research Report, (7), 1-11.
Miranda, L. M. B., Goulart, C. C., Leite, S. C. B., Batista, A. S. M., & Lima, R. C. (2017). Farelo de algodão em dietas com ou sem suplementação de enzimas para frangos de corte. Revista Ciência Agronômica, 48(4), 690-699. doi: 10.5935/1806-6690.20170080
Moura, F. A. S., Dourado, L. R. B., Farias, L. A., Lopes, J. B., Lima, S. B. P., & Fernandes, M. L. (2019). Complexos enzimáticos sobre a energia metabolizável e digestibilidade dos nutrientes do milheto para frangos de corte. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 71(3), 990-996. doi: 10.1590/ 1678-4162-10021
Munyaka, P. M., Nandha, N. K., Kiarie, E., Nyachoti, C. M., & Khafipour, E. (2016). Impact of combined β-glucanase and xylanase enzymes on growth performance, nutrients utilization and gut microbiota in broiler chickens fed corn or wheat-based diets. Poultry Science, 95(3), 528-540. doi: 10.3382/ps/pev333
Noy, Y., & Sklan, D. (2002). Nutrient use in chicks during the first week posthatch. Poultry Science, 81(3), 391-399. doi: 10.1093/ps/81.3.391
Ribeiro, J. S., Fassani, E. J., Makiyama, L., & Clemente, A. H. S. (2015). Suplementação de enzimas amilase, fitase e protease para codornas japonesas em postura. Boletim de Indústria Animal, 72(2), 163-169. doi: 10.17523/bia.v72n2p163
Rostagno, H. S., Albino, L. F. T., Donzele, J. L., Gomes, P. C., Oliveira, R. F., Lopes, D. C.,… Euclides, R. F. (2011). Tabelas brasileiras para aves e suínos: composição de alimentos e exigências nutricionais. Viçosa: Editora da Universidade Federal de Viçosa.
Sakomura, N. K., & Rostagno, H. S. (2016). Métodos de pesquisa em nutrição de monogástricos. São Paulo: Editora FUNEP.
Shirmohammad, F., & Mehri, M. (2011). Effects of dietary supplementation of multi-enzyme complex on the energy utilization in rooster and performance of broiler chicks. African Journal of Biotechnology, 10(38), 7541-7547. doi: 10.5897/AJB10.2260
Silva, D. J., & Queiroz, A. C. (2002). Análise de alimentos: métodos químicos e biológicos. Viçosa, MG: Editora da Universidade Federal de Viçosa.
Tang, D., Hao, S., Liu, G., Nian, F., & Ru, Y. (2014). Effects of maize source and complex enzymes on performance and nutrient utilization of broilers. Asian-Australasian Journal of Animal Science, 27(12), 1755-1762. doi: 10.5713/ajas.2014.14255
Walk, C. L., Juntunen, K., Paloheimo, M., & Ledoux, D. R. (2019). Evaluation of novel protease enzymes on growth performance and nutriente digestibility of poultry: enzyme dose response. Poultry Science, 98(11), 5525-5532. doi: 10.3382/ps/pez299
Yuan, L., Wang, M., Zhang, X., & Wang, Z. (2017). Effects of protease and non-starch polysaccharide enzyme on performance, digestive function, activity and gene expression of endogenous enzyme of broilers. Plos One, 12(3), 1-13. doi: 10.1371/journal.pone.0173941
Zou, J., Zheng, P., Zhang, K., Ding, X., & Bai, S. (2013). Effects of exogenous enzymes and dietary energy on performance and digestive physiology of broilers. Journal of Animal Science and Biotechnology, 4(14), 1-9. doi: 10.1186/2049-1891-4-14
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Published
2021-07-02
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Santos Neto, L. D. dos, Martins, J. M. da S., Carvalho, G. B. de, Jardim Filho, R. M., Stringhini, J. H., Oliveira, H. F. de, & Café, M. B. (2021). Use of different combinations of enzyme complexes in broiler diets. Semina: Ciências Agrárias, 42(5), 2907–2924. https://doi.org/10.5433/1679-0359.2021v42n5p2907
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