In vitro and in vivo parameters for identification of landrace pigs with low reproductive performance
DOI:
https://doi.org/10.5433/1679-0359.2022v43n2p573Keywords:
Genetic improvement, Pigs, Semen, Subfertility.Abstract
In pig farming, measurements of production parameters play a fundamental role in the success of the activity. Minimal differences in fertility between breeders can lead to less reproductive efficiency and, less productivity. However, assessing the fertility of each male and the early identification of subfertile males is a difficult task to be performed. Thus, the aim of this study was to evaluate the use of in vitro and in vivo parameters in the identification of subfertile males of the Landrace breed, aiming to collaborate with genetic improvement programs, routine optimization in the Genetic Diffusion Units (GDUs) and the results of performance. In experiment 1, an approach to identify males with subfertility was evaluated based on retrospective data. For this, the results (averages of birth rates, number of total births and average percentages of female and male piglets per litter) were evaluated for a total of 996 matings and 847 parturitions. The inseminations came from ejaculates of 32 males, who had at least 19 females inseminated with homospermic doses in the concentration of 2.5 x 109 total sperm from the same male. As for the birth rate (BR), an average of 85.47% ± 6.05 was observed with a group of median males, seven males that stood out and one individual (M32) with a performance of 58.06% ± 9.0. For the total number of piglets born (PB) the average was 13.41 ± 0.56, with three males with better performance and one (M32) with very poor performance (8.62 ± 0.59). In experiment 2, it was verified whether evaluations of inseminating doses (ID) of semen in vitro (motility and sperm morphology) after 96 hours of storage had correlations with fertility in vivo, which can be used to identify subfertile males. The evaluations were performed on 30 ejaculates regarding the means of BR and PB, considering only those who had at least 7 females inseminated. There were no correlations between the motility assessments and semen morphological changes and the reproductive parameters evaluated. The results obtained in vivo, referring to BR and PB, demonstrated that it was possible to identify differences between males, the individual (M32) had the worst results for the percentages of BR and PB. It is concluded that there are males of high and low fertility and that only the in vitro analyzes carried out in this study are not enough to categorize them, however, the evaluation of retrospective data was efficient for this purpose.Downloads
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References
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Yeste, M. (2016). Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs. Theriogenology, 85(1), 47-64. doi: 10.1016/j.theriogenology.2015.09.047
Arruda, R. P., Geleghini, E. C. C., Garcia, A. R., Santos, G. C., Leite, T. G., Oliveira, L. Z., Rodrigues, M. P. (2015). Morfologia espermática de touros: interpretação e impacto na fertilidade. Revista Brasileira de Reprodução Animal, 39(1), 47-60.
Broekhuijse, M., Feitsma, H., & Gadella, B. M. (2011). Field data analyses of boar semen quality. Reproduction in Domestic Animals, 46(2), 59-63. doi: 10.1111/j.1439-0531.2011. 01861.x
Dyck, M. K., Foxcroft, G. R., Novak, S., Ruiz-Sanchez, A., Patterson, J., & Dixon, W. T. (2011). Biological markers of boar fertility. Reproduction in Domestic Animals, 46(2), 55-58. doi: 10.1111/j.1439-0531. 2011. 01837.x
Ferreira, C. E. R., Moreira, F., Goularte, K. L., Gasperin, B. G., Bianchi, I., Lucia, T. Jr., & Corcini, C. D. (2014). Características seminais de machos suínos no sul do Brasil: estudo retrospectivo. Revista Acadêmica Ciências Agrárias e Ambientais, 12(4), 243-248. doi: 10.7213/academica.12.04.AO02
Jung, M., Rüdiger, K., & Schulze, M. (2015). In vitro measures for assessing boar semen fertility. Reproduction in Domestic Animals, 50(2), 20-24. doi: 10.1111/rda.12533
Knox, R. V. (2013). Impact of swine reproductive technologies on pig and global food production. In G. C. Lamb, & N. DiLorenzo (Eds.), Current and future reproductive technologies and world food production. Advances in experimental medicine and biology (pp. 131-160). New York, NY: Springer. Retrieved from https://link.springer.com/chapter/10.1007/978-1-4614-8887-3_7
Knox, R. V. (2016). Artificial insemination in pigs today. Theriogenology, 85(1), 83-93. doi: j.theriogenology.2015.07.009
Kwon, W., Rahman, M. S., Lee, J., Yoon, S., Park, Y., & Pang, M. G. (2015). Discovery predictive biomarkers for litter size in boar spermatozoa. Molecular & Celular Proteomics, 14(5), 1230-1240. doi: 10.1074/mcp.M114.045369
Maes, D., Soom, A. V., Appeltant, R., Arsenakis, I., & Nauwynck, H. (2016). Porcine semen as a vector for transmission of viral pathogens. Theriogenology, 85(1), 27-38. doi: 10.1016/j.theriogenology.2015.09. 046
Menezes, T. A., Mellagi, A. P. G., Oliveira, G. S., Bernardi, M. L., Wentz, I., Ulguim, R. R., & Bortolozzo, F. P. (2020). Antibiotic-free extended boar semen preserved under low temperature maintains acceptable in-vitro sperm quality and reduces bacterial load. Theriogenology, 149, 131-138. doi: 10.1016/j.theriogenology.2020.03.003 009
Moreira, F., Ferreira, C. E. R., Panzardi, A., & Corcini, C. D. (2013). Técnicas de inseminação artificial e uso de diferentes doses inseminantes em suínos. Science and Animal Health, 1(1), 50-69. doi: 10.15210/ sah.v1i1.485
Pérez-Patiño, C., Parrila, I., Barranco, I., Vergara-Barberan, M., Simó-Alfonso, E. F.,... Roca, J. (2018). New in-depth analytical approach of the porcine seminal plasma proteome reveals potential fertility biomarkers. Journal of Proteome Research, 17(3), 1065-1076. doi: 10.1021/acs.jproteome.7b00728
Rahman, M. S., Kwon, W., & Pang, M. (2017). Protections of male fertility using capacitation-associated proteins in spermatozoa. Molecular Reproduction and Development, 84(9), 749-759. doi: 10.1002/mrd. 22810
Roca, J., Broekhuijse, M. L. W. J., Parrilla, I., Rodriguez-Martinez, H., Martinez, E. A., & Bolarin, A. (2015). Boar differences in artificial insemination outcomes: can they be minimized? Reproduction in Domestic Animals, 50(2), 48-55. doi: 10.1111/rda.12530
Yeste, M. (2016). Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs. Theriogenology, 85(1), 47-64. doi: 10.1016/j.theriogenology.2015.09.047
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Published
2022-02-25
How to Cite
Málaga, F. da C., Siqueira, H. A., Rauber, L. P., Marques, M. G., Peripolli, V., Schwegler, E., … Bianchi, I. (2022). In vitro and in vivo parameters for identification of landrace pigs with low reproductive performance. Semina: Ciências Agrárias, 43(2), 573–584. https://doi.org/10.5433/1679-0359.2022v43n2p573
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