Ocimum gratissimum essential oil improved the health, innate immunity and resistance to Aeromonas hydrophila infection in Pseudoplatystoma reticulatum
DOI:
https://doi.org/10.5433/1679-0359.2021v42n6SUPL2p3855Keywords:
Bacterial experimental infection, Blood alterations, Immunological response, Native fish, Performance.Abstract
The essential oil of alfavaca (Ocimum gratissimum) contains important compounds, such as 1.8-cineol and eugenol. This study evaluated the effects of diets containing alfavaca essential oil on the zootechnical performance, plasma glucose, leukocyte respiratory activity, haematology, and intestinal histomorphometry in “cachara” (Pseudoplatystoma reticulatum) challenged with Aeromonas hydrophila. A total of 300 juvenile fish, with an average total length of 14.98 ± 0.28 cm and average weight of 18.84 ± 1.22 g, distributed in 20 tanks of 80 L, were fed twice a day with a diet containing essential oil at 0.5, 1.0, or 1.5% or without its inclusion in five replicates. After 45 days, blood collection and mid-intestinal bowel were sampled, before a challenge with Aeromonas hydrophila was performed. The fish supplemented with 1.0% of essential oil presented better weight gain, mean corpuscular volume, concentration of thrombotic and neutrophils, respiratory activity of leukocytes, and height of villi (p < 0.05) when compared to other groups. The cumulative mortality response was lower for fish fed 1.5% essential oil of alfavaca diets. Thus, the use of alfavaca essential oil is recommended in the diet of cachara catfish (P. reticulatum) at the level of up to 1.0% for 45 days for improvements in the zootechnical, haematological, and intestinal histomorphometric parameters.Downloads
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References
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Azevedo, T. M. P., Albinati, R. C. B., Guerra-Santos, B., Pinto, L. F. B., Lira, A. D., Medeiros, S. D. C., & Ayres, M. C. C. (2016). Valores de referência dos parâmetros hematológicos de Oreochromis niloticus (Linaeus, 1758) cultivados em tanques-rede em Paulo Afonso, no estado da Bahia, Brasil. Brazilian Journal of Aquatic Science and Technology, 20(2), 63-74. doi: 10.14210/bjast.v20n2.4588
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Boaventura, T. P., Souza, C. F., Ferreira, A. L., Favero, G. C., Baldissera, M. D., Heinzmann, B. M.,... Luz, R. K. (2021). The use of Ocimum gratissimum L. essential oil during the transport of Lophiosilurus alexandri: Water quality, hematology, blood biochemistry and oxidative stress. Aquaculture, 531. doi: 10.1016/j.aquaculture.2020.735964
Brum, A., Pereira, S. A., Cardoso, L., Chagas, E. C., Chaves, F. C. M., Mouriño, J. L. P., & Martins, M. L. (2018). Blood biochemical parameters and melanomacrophage centers in Nile tilapia fed essential oils of clove basil and ginger. Fish and Shellfish Immunology, 74, 444-449. doi: 10.1016/j.fsi.2018.01.021
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Claudiano, G. S., Yunis-Aguinaga, J., Marinho, F. A., Neto, Miranda, R. L., Martins, I. M., Otani, F. S.,... Moraes, J. R. E. (2019). Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophila. Fish and Shellfish Immunology, 88, 259-265. doi: 10.1016/j.fsi. 2019.01.044
Collier, H. B. (1944). The standardizations of blood haemoglobin determinations. Canadian Medical Association Journal, 50(6), 550-552.
Dairiki, J. K., Majolo, C., Chagas, E. C., Chaves, F. C. M., Oliveira, M. R., & Morais, I. S. (2013). Procedimento para inclusão de óleos essenciais em rações para peixes. (Circular Técnica, 42). Manaus: EMBRAPA Amazônia Ocidental.
Elsheshtawy, A., Yehia, N., Elkemary, M., & Soliman, H. (2018). Direct detection of unamplified Aeromonas hydrophila DNA in clinical fish samples using gold nanoparticle probe-based assay. Aquaculture, 500, 451-457. doi: 10.1016/j.aquaculture.2018.10.046
Fantini, L. E., Corrêa, R. A. C. C., Fº., Martins, T. X., Laice, L. M., Seraphim, G. N., Silva, A. L. N.,... Povh, J. A. (2019). Growth curve comparison of native fish cachara (Pseudoplatystoma reticulatum) and cachapinta hybrid (P. reticulatum × Pseudoplatystoma corruscans). Aquaculture, 506, 70-74. doi: 10.10 16/j.aquaculture.2019.03.020
Fernandes, V. F., Almeida, L. B., Feijó, E. V. R. S., Silva, D. C., Oliveira, R. A., Mielke, M. S., & Costa, L. C. B. (2013). Light intensity on growth, leaf micromorphology and essential oil production of Ocimum gratissimum. Revista Brasileira de Farmacognosia, 23(3), 419-424. doi: 10.1590/S0102-695X2013005 000041
Fries, E. M., Zaminhan, M., Luchesi, J. D., Costa, J. M., Maluf, M. L. F., Signor, A.,... Feiden, A. (2013). Revista Brasileira de Ciência Veterinária, 20(2), 84-88. doi: 10.4322/rbcv.2014.054
Goldenfarb, P. B., Bowyer, F. P., Hall, E., & Brosious, E. (1971). Reproducibility in the hematology laboratory: the microhematocrit determinations. American Journal of Clinical Pathology, 56(1), 35-39. doi: 10.1093/ajcp/56.1.35
Honorato, C. A., Caneppele, A., Matoso, J. C., Prado, M. R., Siqueira, M. S., & Souza, L. R. O. (2014) Caracterização física de filés de surubim (Pseudoplatystoma sp.), pacu (Piaractus mesopotamicus) e pirarucu (Arapaimas gigas). Arquivos de Ciências Veterinária e Zoologia da UNIPAR, 17(4), 237-242. doi: 10.25110/arqvet.v17i4.2014.5023
Meneses, J. O., Couto, M. V. S. do, Souza, N. C., Cunha, F. S., Abe, A. H., Ramos, F. M.,... Fujimoto, C. Y. (2018). Efficacy of Ocimum gratissimum essential oil against the monogenean Cichlidogyrus tilapiae gill parasite of Nile tilapia. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 70(2), 497-504. doi: 10.1590/1678-4162-9667
Mohamed, M. A., El-Daly, E. F., Abd El-Azeem, N. A., Youssef, A. W., & Hassab, H. M. A. (2014). Growth performance and histological changes in ileum and immune related organs of broilers fed organic acids or antibiotic growth promoter. International Journal of Poultry Science, 13(10), 602-610. doi: 10.3923/ijps.2014.602.610
Monteiro, P. C., Brandão, F. R., Farias, C. F. S., Sebastião, F. A., Majolo, C., Dairiki, J. K.,… Chagas, E. C. (2021). Dietary supplementation with essential oils of Lippia sidoides, Ocimum gratissimum and Zingiber officinale on the growth and hemato-immunological parameters of Colossoma macropomum challenged with Aeromonas hydrophila. Aquaculture Reports, 19(100561). doi: 10.1016/j.aqrep.2020.1 00561
Nunes, L. A., Owatari, M. S., Rodrigues, R. A., Fantini, L. E., Kasai, R. Y. D., Martins, M. L.,… Campos, C. M. (2020). Effects of Bacillus subtilis C-3102-supplemented diet on growth, non-specific immunity, intestinal morphometry and resistance of hybrid juvenile Pseudoplatystoma sp. Challenged with Aeromonas hydrophila. Aquaculture International, 28(6), 2345-2361. doi: 10.1007/s.10499-020-00586-1
Rebouças, P. M., Lima, L. R., Dias, I. F., & Barbosa, J. A. Fº. (2014). Influência da oscilação térmica na água da piscicultura. Journal of Animal Behaviour and Biometeorology, 2, 35-42.
Ribeiro, A. S., Batista, E. dos S., Dairiki, J. K., Chaves, F. C. M., & Inoue, L. A. K. A. (2016). Anesthetic properties of Ocimum gratissimum essential oil for juvenile matrinxã. Acta Scientiarum Animal Sciences, 38(1), 1-7. doi: 10.4025/actascianimsci.v38i1.28787
Rodrigues, R. A., Nunes, C. S., Fantini, L. E., Kassai, R. Y. D., Oliveira, C. A. L., Hisano, H., & Campos, C. M. (2018). Dietary ascorbic acid influences the intestinal morphology and hematology of hybrid sorubim catfish (Pseudoplatystoma reticulatum x P. corruscans). Aquaculture International, 26(1), 1-11. doi: 10.1007/s10499-017-0188-0
Silva, B. C., Mouriño, J. L. P., Vieira, F. N., Jatobá, A., Seiffert, W. Q., & Martins, M. L. (2011). Haemorrhagic septicaemia in the hybrid surubim (Pseudoplatystoma corruscans x Pseudoplatystoma fasciatum) caused by Aeromonas hydrophila. Aquaculture Research, 43, 908-916. doi: 10.1111/j.1365-2109.2011.02905.x
Silva, L. L., Parodi, T. V., Reckziege, L. P., Garcia, V. O., Bürger, M. E., Baldisserotto, B.,… Heinzmann, B. M. (2012). Essential oil of Ocimum gratissimum L.: Anesthetic effects, mechanism of action and tolerance in silver catfish, Rhamdia quelen. Aquaculture, 350-353, 91-97. doi: 10.1016/j.aquaculture. 2012.04.012
Tavares-Dias, M., & Moraes, F. R. (2004). Hematologia de peixes teleósteos. Ribeirão Preto: M. Tavares-Dias.
Valladão, G. R. M., Gallani, S. U., & Pilarski, F. (2015). Phytotherapy as an alternative for treating fish disease. Journal of Veterinary Pharmacology and Therapeutics, 38(5), 417-428. doi: 10.1111/jvp.12202
Valladão, G. R. M., Gallani, S. U., Ikefuti, C. V., Cruz, C., Levy-Pereira, N., Rodrigues, M. V. N., & Pilarski, F. (2016). Essential oils to control ichthyophthiriasis in pacu, Piaractus mesopotamicus (Holmberg): special emphasis on treatment with Melaleuca alternifolia. Journal of Fish Diseases, 39(10), 1143-1152. doi: 10.1111/jfd.12447
Van Den Dool, H., & Kratz, P. (1963) A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. Journal of Chromatography, 11, 463-471. doi: 10.1016/S0021-9673(01)80947-X
Wintrobe, M. M. (1934). Variations in the size and hemoglobin content of erythrocytes in the blood of various vertebrates. Folia Haematologica, 51, 32-49.
Adams, R. P. (2007). Identification of essential oils components by gas chromatography/mass spectrometry. Illinois: Allured Publishing Corp.
Alves, L. A., Varela, E. S., Moro, G. V., & Kirschnik, L. N. G. (2014). Riscos genéticos da produção de híbridos de peixes nativos. (Documentos, 3). Palmas: Empresa Brasileira de Pesquisa Agropecuária.
Anderson, D. P., & Siwicki, A. K. (1995). Basic hematology and serology for fish health programs. In M. Shariff, J. R. Arthur, & R. P. Subasinghe, Diseases in Asian aquaculture II (pp. 185-202). Manila: Fish Health Section, Asian Fisheries Society.
Azevedo, T. M. P., Albinati, R. C. B., Guerra-Santos, B., Pinto, L. F. B., Lira, A. D., Medeiros, S. D. C., & Ayres, M. C. C. (2016). Valores de referência dos parâmetros hematológicos de Oreochromis niloticus (Linaeus, 1758) cultivados em tanques-rede em Paulo Afonso, no estado da Bahia, Brasil. Brazilian Journal of Aquatic Science and Technology, 20(2), 63-74. doi: 10.14210/bjast.v20n2.4588
Baumgartner, G., Pavanelli, C. S., Baumgartner, D., Bifi, A. G., Debona, T., & Frana, V. A. (2012). Peixes do baixo rio Iguaçu. Maringá: Eduem.
Boaventura, T. P., Souza, C. F., Ferreira, A. L., Favero, G. C., Baldissera, M. D., Heinzmann, B. M.,... Luz, R. K. (2021). The use of Ocimum gratissimum L. essential oil during the transport of Lophiosilurus alexandri: Water quality, hematology, blood biochemistry and oxidative stress. Aquaculture, 531. doi: 10.1016/j.aquaculture.2020.735964
Brum, A., Pereira, S. A., Cardoso, L., Chagas, E. C., Chaves, F. C. M., Mouriño, J. L. P., & Martins, M. L. (2018). Blood biochemical parameters and melanomacrophage centers in Nile tilapia fed essential oils of clove basil and ginger. Fish and Shellfish Immunology, 74, 444-449. doi: 10.1016/j.fsi.2018.01.021
Brum, A., Pereira, S. A., Owatari, M. S., Chagas, E. C., Chaves, F. C. M., Mouriño, J. L. P., & Martins, M. L. (2017). Effect of dietary essential oils of clove basil and ginger on Nile tilapia (Oreochromis niloticus) following challenge with Streptococcus agalactiae. Aquaculture, 468(1), 235-243. doi: 10.101 6/j.aquaculture.2016.10.020
Claudiano, G. S., Yunis-Aguinaga, J., Marinho, F. A., Neto, Miranda, R. L., Martins, I. M., Otani, F. S.,... Moraes, J. R. E. (2019). Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophila. Fish and Shellfish Immunology, 88, 259-265. doi: 10.1016/j.fsi. 2019.01.044
Collier, H. B. (1944). The standardizations of blood haemoglobin determinations. Canadian Medical Association Journal, 50(6), 550-552.
Dairiki, J. K., Majolo, C., Chagas, E. C., Chaves, F. C. M., Oliveira, M. R., & Morais, I. S. (2013). Procedimento para inclusão de óleos essenciais em rações para peixes. (Circular Técnica, 42). Manaus: EMBRAPA Amazônia Ocidental.
Elsheshtawy, A., Yehia, N., Elkemary, M., & Soliman, H. (2018). Direct detection of unamplified Aeromonas hydrophila DNA in clinical fish samples using gold nanoparticle probe-based assay. Aquaculture, 500, 451-457. doi: 10.1016/j.aquaculture.2018.10.046
Fantini, L. E., Corrêa, R. A. C. C., Fº., Martins, T. X., Laice, L. M., Seraphim, G. N., Silva, A. L. N.,... Povh, J. A. (2019). Growth curve comparison of native fish cachara (Pseudoplatystoma reticulatum) and cachapinta hybrid (P. reticulatum × Pseudoplatystoma corruscans). Aquaculture, 506, 70-74. doi: 10.10 16/j.aquaculture.2019.03.020
Fernandes, V. F., Almeida, L. B., Feijó, E. V. R. S., Silva, D. C., Oliveira, R. A., Mielke, M. S., & Costa, L. C. B. (2013). Light intensity on growth, leaf micromorphology and essential oil production of Ocimum gratissimum. Revista Brasileira de Farmacognosia, 23(3), 419-424. doi: 10.1590/S0102-695X2013005 000041
Fries, E. M., Zaminhan, M., Luchesi, J. D., Costa, J. M., Maluf, M. L. F., Signor, A.,... Feiden, A. (2013). Revista Brasileira de Ciência Veterinária, 20(2), 84-88. doi: 10.4322/rbcv.2014.054
Goldenfarb, P. B., Bowyer, F. P., Hall, E., & Brosious, E. (1971). Reproducibility in the hematology laboratory: the microhematocrit determinations. American Journal of Clinical Pathology, 56(1), 35-39. doi: 10.1093/ajcp/56.1.35
Honorato, C. A., Caneppele, A., Matoso, J. C., Prado, M. R., Siqueira, M. S., & Souza, L. R. O. (2014) Caracterização física de filés de surubim (Pseudoplatystoma sp.), pacu (Piaractus mesopotamicus) e pirarucu (Arapaimas gigas). Arquivos de Ciências Veterinária e Zoologia da UNIPAR, 17(4), 237-242. doi: 10.25110/arqvet.v17i4.2014.5023
Meneses, J. O., Couto, M. V. S. do, Souza, N. C., Cunha, F. S., Abe, A. H., Ramos, F. M.,... Fujimoto, C. Y. (2018). Efficacy of Ocimum gratissimum essential oil against the monogenean Cichlidogyrus tilapiae gill parasite of Nile tilapia. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 70(2), 497-504. doi: 10.1590/1678-4162-9667
Mohamed, M. A., El-Daly, E. F., Abd El-Azeem, N. A., Youssef, A. W., & Hassab, H. M. A. (2014). Growth performance and histological changes in ileum and immune related organs of broilers fed organic acids or antibiotic growth promoter. International Journal of Poultry Science, 13(10), 602-610. doi: 10.3923/ijps.2014.602.610
Monteiro, P. C., Brandão, F. R., Farias, C. F. S., Sebastião, F. A., Majolo, C., Dairiki, J. K.,… Chagas, E. C. (2021). Dietary supplementation with essential oils of Lippia sidoides, Ocimum gratissimum and Zingiber officinale on the growth and hemato-immunological parameters of Colossoma macropomum challenged with Aeromonas hydrophila. Aquaculture Reports, 19(100561). doi: 10.1016/j.aqrep.2020.1 00561
Nunes, L. A., Owatari, M. S., Rodrigues, R. A., Fantini, L. E., Kasai, R. Y. D., Martins, M. L.,… Campos, C. M. (2020). Effects of Bacillus subtilis C-3102-supplemented diet on growth, non-specific immunity, intestinal morphometry and resistance of hybrid juvenile Pseudoplatystoma sp. Challenged with Aeromonas hydrophila. Aquaculture International, 28(6), 2345-2361. doi: 10.1007/s.10499-020-00586-1
Rebouças, P. M., Lima, L. R., Dias, I. F., & Barbosa, J. A. Fº. (2014). Influência da oscilação térmica na água da piscicultura. Journal of Animal Behaviour and Biometeorology, 2, 35-42.
Ribeiro, A. S., Batista, E. dos S., Dairiki, J. K., Chaves, F. C. M., & Inoue, L. A. K. A. (2016). Anesthetic properties of Ocimum gratissimum essential oil for juvenile matrinxã. Acta Scientiarum Animal Sciences, 38(1), 1-7. doi: 10.4025/actascianimsci.v38i1.28787
Rodrigues, R. A., Nunes, C. S., Fantini, L. E., Kassai, R. Y. D., Oliveira, C. A. L., Hisano, H., & Campos, C. M. (2018). Dietary ascorbic acid influences the intestinal morphology and hematology of hybrid sorubim catfish (Pseudoplatystoma reticulatum x P. corruscans). Aquaculture International, 26(1), 1-11. doi: 10.1007/s10499-017-0188-0
Silva, B. C., Mouriño, J. L. P., Vieira, F. N., Jatobá, A., Seiffert, W. Q., & Martins, M. L. (2011). Haemorrhagic septicaemia in the hybrid surubim (Pseudoplatystoma corruscans x Pseudoplatystoma fasciatum) caused by Aeromonas hydrophila. Aquaculture Research, 43, 908-916. doi: 10.1111/j.1365-2109.2011.02905.x
Silva, L. L., Parodi, T. V., Reckziege, L. P., Garcia, V. O., Bürger, M. E., Baldisserotto, B.,… Heinzmann, B. M. (2012). Essential oil of Ocimum gratissimum L.: Anesthetic effects, mechanism of action and tolerance in silver catfish, Rhamdia quelen. Aquaculture, 350-353, 91-97. doi: 10.1016/j.aquaculture. 2012.04.012
Tavares-Dias, M., & Moraes, F. R. (2004). Hematologia de peixes teleósteos. Ribeirão Preto: M. Tavares-Dias.
Valladão, G. R. M., Gallani, S. U., & Pilarski, F. (2015). Phytotherapy as an alternative for treating fish disease. Journal of Veterinary Pharmacology and Therapeutics, 38(5), 417-428. doi: 10.1111/jvp.12202
Valladão, G. R. M., Gallani, S. U., Ikefuti, C. V., Cruz, C., Levy-Pereira, N., Rodrigues, M. V. N., & Pilarski, F. (2016). Essential oils to control ichthyophthiriasis in pacu, Piaractus mesopotamicus (Holmberg): special emphasis on treatment with Melaleuca alternifolia. Journal of Fish Diseases, 39(10), 1143-1152. doi: 10.1111/jfd.12447
Van Den Dool, H., & Kratz, P. (1963) A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. Journal of Chromatography, 11, 463-471. doi: 10.1016/S0021-9673(01)80947-X
Wintrobe, M. M. (1934). Variations in the size and hemoglobin content of erythrocytes in the blood of various vertebrates. Folia Haematologica, 51, 32-49.
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2021-10-08
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Silva, L. A. da, Pilarski, F., Chaves, F. C. M., Chagas, E. C., Fernandes, C. E., Libanori, M. C. M., … Campos, C. M. de. (2021). Ocimum gratissimum essential oil improved the health, innate immunity and resistance to Aeromonas hydrophila infection in Pseudoplatystoma reticulatum. Semina: Ciências Agrárias, 42(6SUPL2), 3855–3868. https://doi.org/10.5433/1679-0359.2021v42n6SUPL2p3855
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