Diet and breed alter community structures of rumen protozoa in cattle subjected to different feeding systems
DOI:
https://doi.org/10.5433/1679-0359.2019v40n2p909Keywords:
Breed, Cattle, Isotrichidae, Ophryoscolecidae, Pasture.Abstract
The objective were to identify and quantify the rumen protozoa from two genetic groups of beef cattle (Nellore and crossbred European beef cattle) subjected to three feeding systems (pasture, pasture supplement, and confined). Samples of rumen contents were obtained from the center of the ruminal mass after slaughter. Quantification and identification of rumen protozoa were performed with a Sedgewick-Rafter counting chamber by optic microscopy. Data were analyzed using general linear analysis methodology with a Poisson distribution (1 %). In addition, the data underwent of Analisys Principal Component. A effect on ruminal protozoa was observed for the diets and breeds analyzed. Was identified 14 genera, among which Entodinium was predominant in all animals analyzed. Ciliates belonging to Entodiniomorphida, Eodinium, Epidinium, Eremoplastron, Eudiplodinium, Metadinium, and Ostracodinium showed highest density in Nellore animals when compared with the crossbreed, Nellore × European. Larger numbers of protozoa from the family Isotrichidae (Dasytricha and Isotricha) were observed in animals maintained by pasture or pasture supplement feeding. According to the kind of feeding, those fed exclusively with pasture had a greater diversity of ciliates in comparison to those restricted to, or supplemented with pasture. In one animal, the occurrence of ciliates belonging to the genus Buetschlia, was observed (prevalence of 1.66 %), which is one of only a few records of this genus in ruminants.Downloads
References
ALIPOUR, D. Ruminal ciliated protozoa in baloochi and sindhi camel breeds. Journal of Veterinary Research, Napoli, v. 67, n. 3, p. 257-263, 2012.
ARTMANN, A. T.; TOMA, S. H.; PINHEIRO, N. J.; ROMERO, J.; CARVALHO, A. M.; MONTEIRO, T. Breeding and cattle ½ blood taurino x ½ blood zebu in Brazil. Revista Científica de Medicina Veterinária, Garça, ano XII, n. 22, p. 1-10, 2014.
BONHOMME-FLORENTIN, A. Contribution à l’lètude de La physiologie dês ciliés entodiniomorphes endocommensaux des ruminats et des équidés. Annales des Sciences Naturelles, London, v. 16, n. 12, p. 155-283, 1974.
CEDROLA, F.; MARTINELE, I.; DIAS, R. J. P.; FREGULIA, P.; D’AGOSTO, M. Rumen ciliates in Brazilian sheep (Ovis aries) and redescription of Entodinium contractum (Entodiniomorphida, Ophryoscolecidae). Zootaxa, New Zealand, v. 4088, n. 2, p. 292-300, 2016.
CERSOSIMO, L. M.; BAINBRIDGE, M. L.; WRIGHT, A. D. G.; KRAFT, J. Breed and lactation stage alter the rumen protozoal fatty acid profiles and community structures in primiparous dairy cattle. Journal of Agricultural and Food Chemistry, Washington, v. 64, n. 9, p. 2021-2029, 2016.
D’AGOSTO, M.; CARNEIRO, M. E. Evaluation of lugol solution used for counting rúmen ciliates. Revista Brasileira de Zoologia, Curitiba, v. 16, n. 3, p. 725-729, 1999.
DEHORITY, B. A. Protozoa of the digestive tract of herbivorous mammals. Insect Science and its Applications, Cambridge, v. 7, n. 3, p. 279-296, 1986.
DEHORITY, B. A. Rumen microbiology. Nottinghan: Nottinghan University Press, 2003. 380 p.
GEBIOMET - GRUPO DE ESTUDOS EM BIOMETEOROLOGIA. Dois Vizinhos: Universidade Tecnológica Federal do Paraná, 2014. Disponível em: www.gebiomet.com.br. Acesso em: 20 jan. 2015.
HUWS, S. A.; KIM, E. J.; KINGSTON-SMITH, A. H.; LEE, M. R. F.; MUETZEL, S. M.; COOKSON, A. R.; NEWBOLD, C. J.; WALLACE, R. J.; SCOLLAN, N. D. Rumen protozoa are rich in polyunsaturated fatty acids due to the ingestion of chloroplasts. FEMS Microbiology Ecology, Bethesda, v. 69, n. 3, p. 461-471, 2009.
MANELLA, M. Q.; LOURENÇO, A. J. População de protozoários ciliados no rúmen de bovinos nelore em pastos de Brachiaria brizantha Marandu recebendo suplemento protéico ou com livre acesso a banco de proteína de Leucaena leucocephala nas diferentes estações do ano. Boletim de Indústria Animal, Nova Odessa, v. 61, n. 1, p. 1-11, 2004.
MARTINELE, I.; SANTOS, G. R. A.; MATOS, D. S.; BATISTA, A. M. V.; D’AGOSTO, M. Diet botanical composition and rumen protozoa of sheep in brazilian semi-arid. Archivos de Zootecnia, Cordoba, v. 59, n. 226, p. 169-175, 2010.
MARTINELE, I.; SANTOS, G. R. A.; MATOS, D. S.; BATISTA, A. M. V.; D’AGOSTO, M. Protozoários ciliados no rúmen de ovinos mestiços mantidos em pastagem natural de caatinga. Revista Brasileira de Saúde e Produção Animal, Salvador, v. 9, n. 2, p. 280-292, 2008.
NEWBOLD, C. J.; de la FUENTE, G.; BELANCHE, A.; RAMOS-MORALES, E.; MCEWAN, N. R. The role of ciliate protozoa in the rumen. Frontiers in Microbiology, Lausanne, v. 1313, n. 6, p. 1-7, 2015.
OGIMOTO, K.; IMAI, S. Atlas of rumen microbiology. Tokyo: Japan Scientificv Societies, 1981. 268 p.
O'KELLY, J. C.; SPIERS, W. G. Possible contribution of protozoa to diferences in rumen metabolism between cattle breeds. Australian Journal of Agricultural Research, Mailing, v. 43, n. 8, p. 1795-1808, 1992.
SILVA, D. J.; QUEIROZ, A. C. Análises de alimentos: métodos químicos e biológicos. 3. ed. Viçosa, MG: Editora UFV, 2004, 235 p.
SISTEMA PARA ANÁLISES ESTATÍSTICAS - SAEG-. Versão 9.1: Fundação Arthur Bernardes – UFV - Viçosa, MG, 2007.
STATISTICAL ANALYSIS SYSTEM - SAS. System for Microsoft Windows: release 8.2. New York: Cary, 2001.
VOIGT, J.; JENTSCH, W.; KUHLA, S.; MATTHES, H. D.; DERNO, M. Rumen fermentation and retention time of the digesta in growing cattle of the breeds Black-White Dairy Cattle, Galloway, and Highland. Arch Tierz, Dummerstorf, v. 43, n. 6, p. 606-620, 2000.
WILLIAMS, A. G.; COLEMAN, G. S. The rumen protozoa. New York: Springer-Verlag, 1992, 423 p.
WRIGHT, A. D. Rumen protozoa. In: PUNIYA, A. K.; SINGH, R.; KAMRA, D. N. Rumen microbiology: from evolution to revolution. New York: Springer-Verlag, 2015, p. 113-120.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2019 Semina: Ciências Agrárias
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Semina: Ciências Agrárias adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.
