Initial development of upland rice inoculated and co-inoculated with multifunctional rhizobacteria
DOI:
https://doi.org/10.5433/1679-0359.2023v44n1p273Keywords:
Azospirillum brasilense, Bacillus sp. , Growth promotion, Oryza sativa, Sustainability.Abstract
Inoculation and co-inoculation of upland rice with multifunctional rhizobacteria can promote plant growth, especially the root system. Thus, this study aimed to evaluate the effect of inoculation and co-inoculation with Azospirillum sp. and Bacillus sp. in the early development of upland rice. The experiment was conducted using a completely randomized design with 4 treatments and 10 replications, totaling 40 plots. The treatments were: 1) Ab-V5 (Azospirillum brasilense), 2) BRM 63573 (Bacillus sp.), 3) co-inoculation of Ab-V5 + BRM 63573, and 4) control (without rhizobacteria). Inoculation and co-inoculation with the multifunctional rhizobacteria Ab-V5 and BRM 63573 provided positive effects on the initial development of upland rice. Inoculation with isolate BRM 63573 had significant effects on root length, shoot, and total biomass, while inoculation with isolate Ab-V5 had significant effects on root length and production of root and total biomass. Co-inoculation treatment had significant effects on variables such as diameter, volume, total surface, root biomass, and total biomass. The control treatment (without multifunctional rhizobacteria) had the worst results for most of the analyzed variables.
Downloads
References
Alok, S., Ashok, K. S., Vikrant, S., Nivedita, S., Vijay, N. S., Shamin, M. d., Prashant, V. & S.,.Sanjay, S. (2014). Genetic variability among traits associated with grain yield of rice (Oryza sativa L.) exposed to drought at flowering stage. African Journal of Agricultural Research, 9(16), 1252-1264. doi: 10.5897/AJAR2013.7782
Alvarez, R. D. C. F., Habermann, G., Crusciol, C. A. C., Nascente, A. S., & Rodrigues, J. D. (2015). Leaf gas exchange and yield of three upland rice cultivars. Bragantia, 74(1), 1-8. doi: 10.1590/1678-4499.0322
Arsenault J.-L., Pouleur , S., Messier, C., & Guay, R. (1995). WinRHIZO, a root-measuring system with a unique overlap correction method. HortScience, 30(4), 906. doi: 10.21273/HORTSCI.30.4.906D
Babalola, O. O. (2010). Beneficial bacteria of agricultural importance. Biotechnology Letters, 32(11), 1559-1570. doi: 10.1007/s10529-010-0347-0
Bashan, Y., Holguin, G., & De-Bashan, L. E. (2004). Azospirillum-plant relationships: physiological, molecular, agricultural, and environmental advances (1997-2003). Canadian Journal of Microbiology, 50(8), 521-577. doi: 10.1139/w04-035
Biswas, J. C., Ladha, J. K., Dazzo, F. B., Yanni, Y. G., & Rolfe, B. G. (2000). Rhizobial inoculation influences seedling vigor and yield of rice. Agronomy Journal, 92(5), 880-886. doi: 10.2134/agronj2000.925880x
Braga, G. M. B., Jr., Chagas, L. F. B., Amaral, L. R. O., Miller, L. O., & Chagas, A. F. C. Jr. (2018). Efficiency of inoculation by Bacillus Subtilis on soybean biomass and productivity. Revista Brasileira de Ciências Agrárias, 13(4), 1-6. doi:10.5039/agraria.v13i4a5571
Caballero-Mellado, J. (2006). Microbiología agrícola e interacciones microbianas con plantas. Revista Latinoamericana de Microbiología, 48(2), 154-161.
Chi, F., Shen, S. H., Cheng, H. P., Jing, Y. X., Yanni, Y. G., & Dazzo, F. B. (2005). Ascending migration of endophytic rhizobia, from roots to leaves, inside rice plants and assessment of benefits to rice growth physiology. Applied and Environmental Microbiology, 71(11), 7271-7278. doi: 10.1128/AEM.71.11.7271-7278.2005
Coêlho, J. D. (2021). Arroz: produção e mercado.
Cruz, D. R. C., Nascente, A. S., Silva, M. A., & Barroso, J. B., Neto. (2022, April). Root and shoot development of corn seedlings as affected by rhizobacteria. Colloquium Agrariae, 18(1), 53-63. doi: 10.5747/ca.2022.v18.n1.a479
Devkota, K. P., Pasuquin, E., Elmido-Mabilangan, A., Dikitanan, R., Singleton, G. R., Stuart, A. M., Vithoonjit, D., Vidiyangkura, L., Pustika, A. B., Afriani, R, Listyowati, C. L., Keerthisena, R. S. K., Kieu, N. T., Malabayabas, A. J., Hu, R, Pan, J. & Beebout, S. E. (2019). Economic and environmental indicators of sustainable rice cultivation: A comparison across intensive irrigated rice cropping systems in six Asian countries. Ecological Indicators, 105(1), 199-214. doi: 10.1016/j.ecolind.2019.05.029
Dhouib, H., Zouari, I., Abdallah, D. B., Belbahri, L., Taktak, W., Triki, M. A., & Tounsi, S. (2019). Potential of a novel endophytic Bacillus velezensis in tomato growth promotion and protection against Verticillium wilt disease. Biological Control, 139(1), 104092. doi: 10.1016/j.biocontrol.2019.104092
Fernandes, J. P. T., Nascente, A. S., Filippi, M. C. C. D., Lanna, A. C., Sousa, V. S., & Silva, M. A. (2020). Physio-agronomic characterization of upland rice inoculated with mix of multifunctional microorganisms. Revista Caatinga, 33(1), 679-689. doi: 10.1590/1983-21252020v33n311rc
Fernandes, J. P. T., Nascente, A. S., Filippi, M. C. C. de, & Silva, M. A. (2021a). Upland rice seedlings performance promoted by multifunctional microorganisms. Semina: Ciências Agrárias, 42(1), 429-438. doi: 10.5433/1679-0359.2021v42n1p429
Fernandes, J., Nascente, A. S., Filippi, M. C. de, Lanna, A. C., Silva, M. A., & Silva, G. B. (2021b). Effects of beneficial microorganisms on upland rice performance. Revista Brasileira de Engenharia Agrícola e Ambiental, 25(3), 156-162. doi: 10.1590/1807-1929/agriambi.v25n3p156-162
Figueiredo, M. D. V. B., Seldin, L., Araujo, F. F. D., & Mariano, R. D. L. R. (2010). Plant growth promoting rhizobacteria: fundamentals and applications. In D. K., Maheshwari (Ed.), Plant growth and health promoting bacteria (pp. 21-43). Berlin, Heidelberg: Springer.
Filippi, M. C. C., Silva, G. B. da, Silva-Lobo, V. L., Côrtes, M. V. C., Moraes, A. J. G., & Prabhu, A. S. (2011). Leaf blast (Magnaporthe oryzae) suppression and growth promotion by rhizobacteria on aerobic rice in Brazil. Biological Control, 58(2), 160-166. doi: 10.1016/j.biocontrol.2011.04.016
Hungria, M. (2011). Inoculação com Azospirillum brasilense: inovação em rendimento a baixo custo.
Kado, C. I., & Heskett, M. G. (1970). Selective media for isolation of agrobacterium, Corynebacterium, Erwinia, Pseudomonas and Xanthomonas. Phytopathology, 60(6), 969-976.
Korber, L. P. P., Korber, Â. H. C., Grange, L., & Klahold, C. A. (2021). Eficiência de produtos biológicos na coinoculação de sementes de soja. South American Sciences, 2(2), e21109-e21109. doi: 10.52755/sas.v2i2.109
Moreira, F. M. S., & Siqueira, J. O. (2006). Microbiologia e bioquímica do solo (2a ed.). UFLA.
Oliveira, A. A., Neto. (Org.). (2015). A cultura do arroz. CONAB.
Poorter, H., Niklas, K. J., Reich, P. B., Oleksyn, J., Poot, P., & Mommer, L. (2012). Biomass allocation to leaves, stems and roots: metaâ€analyses of interspecific variation and environmental control. New Phytologist, 193(1), 30-50. doi: 10.1111/j.1469-8137.2011.03952.x
Poorter, L. (2001). Light-dependent changes in biomass allocation and their importance for growth of rain forest tree species. Functional Ecology, 15, (1), 113-123.
R Core Team. (2016). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http://www. R-project. org/.
Santos Dias, A. dos, & Santos, C. C. (2022). Bactérias promotoras de crescimento de plantas: conceitos e potencial de uso. Pantanal.
Santos Lopes, M. J. dos, Dias, M. B. Fº., Reis Castro, T. H. dos, Gurgel, E. S. C., & Silva, G. B. da. (2021a). Efficiency of biostimulants for alleviating shade effects on forage grass. Journal of Agricultural Studies, 9(3), 14-30. doi: 10.5296/jas.v9i3.18611
Santos Lopes, M. J. dos, Santiago, B. S., Silva, I. N. B. da, & Gurgel, E. S. C. (2021b). Biotecnologia microbiana: inoculação, mecanismos de ação e benefícios às plantas. Research, Society and Development, 10(12), e356101220585-e356101220585. doi: 10.33448/rsd-v10i12.20585
Silva, L. L. D. (2017). Caracterização bioquímica e molecular de bactérias associadas a Oryza sativa L. produtoras de biocompostos.
Silva, A. P. da, & Dourado, M. N. (2022a). Mecanismos de promoção de crescimento vegetal por bactérias endofíticas. Iniciação Científica Cesumar, 24(1), 1-11. doi: 10.17765/2176-9192.2022v24n1e10776
Silva, J. A. N. da, Iacia, J. C., Forest, R., & Silva, M. L. S. da. (2022b). Manutenção e persistência do palhiço em função do manejo e seu efeito sobre o sistema radicular da cana-de-açúcar. Revista Magsul de Agronomia, 1-15. https://magsulnet.magsul-ms.com.br/revista/index.php/rma/article/view/78
Silva, M. A., Nascente, A. S., Filippi, M. C. C. D., Lanna, A. C., Silva, G. B. D., & Silva, J. F. A. E. (2020). Individual and combined growth-promoting microorganisms affect biomass production, gas exchange and nutrient content in soybean plants. Revista Caatinga, 33(3), 619-632. doi: 10.1590/1983-21252020v33n305rc
Silveira, A. P. D. da, & Freitas, S. dos Santos. (2007). Microbiota do solo e qualidade ambiental. Instituto Agronômico.
Taiz, L., & Zeiger, E. (2009). Fisiologia vegetal. In L. Taiz, & E. Zeiger (Eds.), Fisiologia vegetal (pp. 1-848). Porto Alegre.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Semina: Ciências Agrárias
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Semina: Ciências Agrárias adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.
