Is the proAKAP4 a suitable biomarker of X-sorted sperm quality from Nelore and Gir bulls?
DOI:
https://doi.org/10.5433/1679-0359.2024v45n5p1413Keywords:
AKAP4 protein, Non-sorted sperm, Sex-sorted sperm, X chromosome.Abstract
ProAKAP4 is a protein precursor of AKAP4 present in the principal piece of the flagellum, associated with sperm motility. It has been described in several species, but its concentration has never been measured in sex-sorted sperm. Thus, the study aimed to test the proAKAP4 as a suitable sperm quality biomarker for Bos indicus X-sorted sperm. To achieve the study’s goal, 14 semen straws from different bulls were evaluated, six X-sorted and eight non-sorted. Sperm kinetics and morpho-functional evaluations were carried out, in addition to the concentration of proAKAP4. The characteristics evaluated were compared by the t-test and correlated by the Pearson’s correlation. A difference was found for total motility (P = 0.014), rapid sperm (P = 0.020), and sperm area (P = 0.013) between the non-sorted and X-sorted sperm. The proAKAP4 concentration was higher in the X-sorted sperm (X-sorted sperm: 67.54 ng/106 spermatozoa; non-sorted sperm: 29.76 ng/106 spermatozoa), but it was not correlated to the kinetics or morpho-functional characteristics evaluated. However, the proAKAP4 in non-sorted sperm showed a correlation to sperm total and progressive motility, rapid sperm, and sperm membrane integrity. The study concluded on the unreliability of the proAKAP4 as a biomarker to indicate sperm quality in the X-sorted sperm.
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Almeida, A. B. M., Megumy, M. H. T., Moraes, L. Z., Trautwein, L. G. C., Schnitzer, J. de F., Silva, L. A. dos S., Rizzoto, G., Ferreira, J. C. P., & Martins, M. I. M. (2022). The proAKAP4 concentrations in Nelore bull sperm and their relation to FTAI conception rate results. Animal Reproduction Science, 247(107), 1-7. doi: 10.1016/j.anireprosci.2022.107156 DOI: https://doi.org/10.1016/j.anireprosci.2022.107156
Bastan, I., & Akcay, E. (2021). Quality assessment of frozen bull semen with the precursor A-kinase anchor protein 4 biomarker. Andrologia, 53(9), 1-8. doi: 10.1111/and.14164 DOI: https://doi.org/10.1111/and.14164
Blommaert, D., Sergeant, N., Delehedde, M., Jouy, N., Mitchell, V., Franck, T., Donnay, I., Lejeune, J. P., & Serteyn, D. (2019). Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology, 131(19), 52-60. doi: 10.1016/j.theriogenology.2019.03.011 DOI: https://doi.org/10.1016/j.theriogenology.2019.03.011
Boro, P., Naha, B. C., Madkar, A., Prakash, C. (2016). Sexing of semen in bulls : a mini review. International Journal of Applied Research of , 2(4), 460-462. https://www.allresearchjournal.com/archives/2016/vol2issue4/PartG/2-3-171.pdf
Brown, P. R., Miki, K., Harper, D. B., & Eddy, E. M. (2003). A-kinase anchoring protein 4 binding proteins in the fibrous sheath of the sperm flagellum. Biology of Reproduction, 68(6), 2241-2248. doi: 10.1095/biolreprod.102.013466 DOI: https://doi.org/10.1095/biolreprod.102.013466
Carvalho, J. de O., Sartori, R., Rodello, L., Mourão, G. B., Bicudo, S. D., & Margot, M. A. (2018). Flow cytometry sex sorting affects bull sperm longevity and compromises their capacity to bind to oviductal cells. Livestock Science, 207(2017), 30-37. doi: 10.1016/j.livsci.2017.11.005 DOI: https://doi.org/10.1016/j.livsci.2017.11.005
De Canio, M., Soggiu, A., Piras, C., Bonizzi, L., Galli, A., Urbani, A., & Roncada, P. (2014). Differential protein profile in sexed bovine semen: shotgun proteomics investigation. Molecular BioSystems, 10(6), 1264-1271. doi: 10.1039/c3mb70306a DOI: https://doi.org/10.1039/C3MB70306A
Dordas-Perpiny, M., Sergeant, N., Ruelle, I., Bruyas, J., Michaud, S., Carracedo, S., Catal, J., Mir, J., & Delehedde, M. (2022). ProAKAP4 semen concentrations as a valuable marker protein of post-thawed semen quality and bull fertility : a retrospective study. Veterinary Sciences, 9(5), 1-11. doi: 10.3390/vetsci9050224 DOI: https://doi.org/10.3390/vetsci9050224
Fang, X., Huang, L. L., Xu, J., Ma, C. Q., Chen, Z. H., Zhang, Z., Liao, C. H., Zheng, S. X., Huang, P., Xu, W. M., Li, N., & Sun, L. (2019). Proteomics and single-cell RNA analysis of Akap4-knockout mice model confirm indispensable role of Akap4 in spermatogenesis. Developmental Biology, 454(2), 118-127. doi: 10.1016/j.ydbio.2019.06.017 DOI: https://doi.org/10.1016/j.ydbio.2019.06.017
González-Marín, C., Góngora, C. E., Gilligan, T. B., Evans, K. M., Moreno, J. F., & Vishwanath, R. (2018). In vitro sperm quality and DNA integrity of SexedULTRATM sex-sorted sperm compared to non-sorted bovine sperm. Theriogenology, 114(14), 40-45. doi: 10.1016/j.theriogenology.2018.03.025 DOI: https://doi.org/10.1016/j.theriogenology.2018.03.025
Jumeau, F., Sigala, J., Dossou-Gbete, F., Frimat, K., Barbotin, A. L., Buée, L., Béhal, H., Sergeant, N., & Mitchell, V. (2018). A-kinase anchor protein 4 precursor (pro-AKAP4) in human spermatozoa. Andrology, 6(6), 854-859. doi: 10.1111/andr.12524 DOI: https://doi.org/10.1111/andr.12524
Morrell, J. M., Valeanu, A. S., Lundeheim, N., & Johannisson, A. (2018). Sperm quality in frozen beef and dairy bull semen. Acta Veterinaria Scandinavica, 60(1), 1-10. doi: 10.1186/s13028-018-0396-2 DOI: https://doi.org/10.1186/s13028-018-0396-2
Riesco, M. F., Anel-Lopez, L., Neila-Montero, M., Palacin-Martinez, C., Montes-Garrido, R., Alvarez, M., Paz, P. de, & Anel, L. (2020). Proakap4 as novel molecular marker of sperm quality in ram: an integrative study in fresh, cooled and cryopreserved sperm. Biomolecules, 10(7), 1-21. doi: 10.3390/biom10071046 DOI: https://doi.org/10.3390/biom10071046
Scott, C., Souza, F. F. de, Aristizabal, V. H. V., Hethrington, L., Krisp, C., Molloy, M., & Baker, M. A. (2018). Proteomic profile of sex-sorted bull sperm evaluated by SWATH- MS analysis. Veterinary Sciences, 198(18), 121-128. doi: 10.1016/j.anireprosci.2018.09.010 DOI: https://doi.org/10.1016/j.anireprosci.2018.09.010
Sergeant, N., Briand-Amirat, L., Bencharif, D., & Delehedde, M. (2019). The sperm specific protein proakap4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences, 11(1), 555-563. doi: 10.19080/jdvs.2019.11.555803 DOI: https://doi.org/10.19080/JDVS.2019.11.555803
Steele, H., Makri, D., Maalouf, W. E., Reese, S., & Kölle, S. (2020). Bovine sperm sexing alters sperm morphokinetics and subsequent early embryonic development. Scientific Reports, 10(1), 1-13. doi: 10.1038/s41598-020-63077-6 DOI: https://doi.org/10.1038/s41598-020-63077-6
Thomas, J. M., Locke, J. W. C., Bonacker, R. C., Knickmeyer, E. R., Wilson, D. J., Vishwanath, R., Arnett, A. M., Smith, M. F., & Patterson, D. J. (2019a). Evaluation of SexedULTRA 4MTM sex-sorted semen in timed artificial insemination programs for mature beef cows. Theriogenology, 123(19), 100-107. doi: 10.1016/j.theriogenology.2018.09.039 DOI: https://doi.org/10.1016/j.theriogenology.2018.09.039
Turner, R. M. O., Johnson, L. R., Haig-Ladewig, L., Gerton, G. L., & Moss, S. B. (1999). An X-linked gene encodes a major human sperm fibrous sheath protein, hAKAP82. Genomic organization, protein kinase A-RII binding, and distribution of the precursor in the sperm tail. Journal of Biological Chemistry, 273(48), 32135-32141. doi: 10.1074/jbc.273.48.32135 DOI: https://doi.org/10.1074/jbc.273.48.32135
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Copyright (c) 2024 Ana Beatriz Marques de Almeida, Myrian Megumy Tsunokawa Hidalgo, Luiz Guilherme Corsi Trautwein , Josiana de Fátima Schnitzer, Letícia Amanda Santos Silva, Fábio Lucas Zito de Moraes, Guilherme Rizzoto, João Carlos Pinheiro Ferreira, Maria Isabel Mello Martins
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