Tuberculosis in bovine slaughtered in the semi-arid region of Rio Grande do Norte, Brazil
DOI:
https://doi.org/10.5433/1679-0359.2021v42n5p3047Keywords:
Mycobacterium bovis, Public health, Slaughterhouses, Tuberculosis.Abstract
Between November 2017 and August 2018, in order to determine the occurrence of tuberculosis in cattle slaughtered in the semi-arid region of Rio Grande do Norte, 11,610 bovine carcasses underwent a routine post-mortem inspection. One animal presented suggestive lesions and samples from lung, spleen and heart were submitted to microbiological culture in Stonebrink medium for 90 days and molecular analysis by nested-PCR. For histopathological examination and Ziehl Neelsen staining, the omentum was used. In the cultured samples, two of them (heart and spleen) showed growth, but were not confirmed as M. bovis by conventional PCR. At nested-PCR, the samples showed amplification for the TbD1 region. The presence of numerous granulomas was detected in the histopathological examination characterized by a necrotic center and areas of mineralization, as well as the presence of acid-fast bacilli (AFB) in the Ziehl-Neelsen stain. Microbiological culture can show false negative results, despite being considered a gold standard technique, although it takes time. Nested-PCR and histopathology show fast and effective results for the diagnosis of the disease. The presence of positive animals represents a public health risk in the studied region. Therefore, one of the essential systems applied to the control of bovine tuberculosis is the epidemiological surveillance of animals in slaughterhouses.Downloads
Download data is not yet available.
References
Araújo, C. P., Osório, A. L. A. R., Jorge, K. S. G., Ramos, C. A. N., Souza, A. F., Fº., Vidal, C. E. S.,... Araújo, F. R. (2014). Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1. Plos One, 9(3), 6. doi: 10.1371/journal.pone.0091023
Bica, R. F. P., Copetti, M. V., & Brum, M. C. S. (2018). Hydatidosis, cysticercosis , and tuberculosis rates in bovine slaughtered under state sanitary inspection in Rio Grande do Sul, Brazil. Ciência Rural, 48(8),1-7. doi: 10.1590/0103-8478cr20170811
Ferreira, J. S., Neto., Silveira, G. B. da S., Rosa, B. M., Gonçalbes, V. S. P., Grisi, J. H., Fº., Amaku, M.,... Lage, A. P. (2016). Analysis of 15 years of the National Program for the Control and Eradication of Animal Brucellosis and Tuberculosis, Brazil. Semina: Ciências Agrárias, 37(5), 3385-3402. doi: 10.54 33/1679-0359.2016v37n5Supl2p3385
Furlanetto, L. V., Figueiredo, E. E. S., Conte, C. A., Jr., Carvalho, R. C. T., Silva, F. G. S., Silva, J. T.,... Paschoalin, V. M. F. (2012). Uso de métodos complementares na inspeção post mortem de carcaças com suspeita de tuberculose bovina. Pesquisa Veterinária Brasileira, 32(11), 1138-1144. doi: 10.1590/ S0100-736X2012001100011
Ghidini, S., Zanardi, E., Di Ciccio, P. A., Borrello, S., Belluzi, G., Guizzardi, S., & Ianieri, A. (2018). Development and test of a visual-only meat inspection system for heavy pigs in Northern Italy. BMC Veterinary Research, 14(1), 1-11. doi: 10.1186/s12917-017-1329-4
Mekonnen, G. A., Conlan, A. J. K., Berg, S., Ayele, B. T., Alemu, A., Guta, S.,… Ameni, G. (2019). Prevalence of bovine tuberculosis and its associated risk factors in the emerging dairy belts of regional cities in Ethiopia. Preventive Veterinary Medicine, 168, 81-89. doi: 10.1016/j.prevet med.2019.04.010
Ministério da Agricultura, Pecuária e Abastecimento (2017). Secretaria de Defesa Animal. Instrução Normativa SDA n°10, de 3 de Março de 2017. Recuperado de https://www.gov.br/agricultura/pt-br/assuntos/sanidade-animal-e-vegetal/saude-animal/programas-de-saude-animal/pncebt/principais-normas-pncebt/in-10-de-3-de-marco-de-2017-aprova-o-regulamento-tecnico-do-pncebt.pdf/view.pdf
Pereira, J. D. B., Cerqueira, V. D., Bezerra, P. S., Bezerra, D. K. O., Araújo, F. R., Dias, A. de C. L.,... Riet-Correa, G. (2017). Diagnóstico histopatológico e molecular de lesões sugestivas de tuberculose em búfalos abatidos nos municípios de macapá e santana, estado do amapá. Pesquisa Veterinária Brasileira, 37(11), 1198-1204. doi: 10.1590/s0100-736x2017001100003
Petroff, S. A. (1915). A new and rapid method for the isolation and cultivation of tubercle bacilli directly from the sputum and feces. Journal of Experimental Medicine, 21(1), 38-42. doi: 10.1084/jem.21.1.38
Ramos, J. M., Heinemann, M. B., Ferreira, J. S., Neto., Souza, A. F., Fº., Cárdenas, N. C., Dantas, A. F. S.,... Azevedo, S. S. (2016). Isolamento e identificação de Mycobacterium bovis em bovinos positivos no teste de tuberculinização no estado da Paraíba, nordeste do Brasil. Arquivos do Instituto Biológico, 85(e0842016), 1-7. doi: 10.1590/18081657000842016
Sales, M. L., Fonseca, A. A., Jr., Sales, É. B., Cottorello, A. C. P., Issa, M. A., Hodon, M. A.,... Heinemann, M. B. (2014). Evaluation of molecular markers for the diagnosis of Mycobacterium bovis. Folia Microbiologica, 59(5), 433-438. doi: 10.1007/s12223-014-0317-3
Secretaria de Planejamento do Rio Grande do Norte (2016). Diagnóstico e planejamento estratégico para o desenvolvimento de atividades produtivas agrícolas do Rio Grande do Norte. Recuperado de http:// www.rnsustentavel.rn.gov.br
Silva, M. R., Rocha, A. da S., Araújo, F. R., Fonseca, A. A., Jr., Alencar, A. P. de, Suffys, P. N.,... Guimarães, M. D. C. (2018). Risk factors for human Mycobacterium bovis infections in an urban area of Brazil. Memórias do Instituto Oswaldo Cruz, 113(8), 1-6. doi: 10.1590/0074-02760170445
Veloso, F. P., Baumgartem, K. D., Mota, A. L. A. D. A., Ferreira, F., Ferreira, J. S., Neto., Grisi, J. H., Fº.,... Gonçalves, V. S. P. (2016). Prevalence and herd-level risk factors of bovine tuberculosis in the State of Santa Catarina. Semina: Ciências Agrárias, 37(5), 3659-3672. doi: 10.5433/1679-0359.2016v37n5 Supl2p3659
Bica, R. F. P., Copetti, M. V., & Brum, M. C. S. (2018). Hydatidosis, cysticercosis , and tuberculosis rates in bovine slaughtered under state sanitary inspection in Rio Grande do Sul, Brazil. Ciência Rural, 48(8),1-7. doi: 10.1590/0103-8478cr20170811
Ferreira, J. S., Neto., Silveira, G. B. da S., Rosa, B. M., Gonçalbes, V. S. P., Grisi, J. H., Fº., Amaku, M.,... Lage, A. P. (2016). Analysis of 15 years of the National Program for the Control and Eradication of Animal Brucellosis and Tuberculosis, Brazil. Semina: Ciências Agrárias, 37(5), 3385-3402. doi: 10.54 33/1679-0359.2016v37n5Supl2p3385
Furlanetto, L. V., Figueiredo, E. E. S., Conte, C. A., Jr., Carvalho, R. C. T., Silva, F. G. S., Silva, J. T.,... Paschoalin, V. M. F. (2012). Uso de métodos complementares na inspeção post mortem de carcaças com suspeita de tuberculose bovina. Pesquisa Veterinária Brasileira, 32(11), 1138-1144. doi: 10.1590/ S0100-736X2012001100011
Ghidini, S., Zanardi, E., Di Ciccio, P. A., Borrello, S., Belluzi, G., Guizzardi, S., & Ianieri, A. (2018). Development and test of a visual-only meat inspection system for heavy pigs in Northern Italy. BMC Veterinary Research, 14(1), 1-11. doi: 10.1186/s12917-017-1329-4
Mekonnen, G. A., Conlan, A. J. K., Berg, S., Ayele, B. T., Alemu, A., Guta, S.,… Ameni, G. (2019). Prevalence of bovine tuberculosis and its associated risk factors in the emerging dairy belts of regional cities in Ethiopia. Preventive Veterinary Medicine, 168, 81-89. doi: 10.1016/j.prevet med.2019.04.010
Ministério da Agricultura, Pecuária e Abastecimento (2017). Secretaria de Defesa Animal. Instrução Normativa SDA n°10, de 3 de Março de 2017. Recuperado de https://www.gov.br/agricultura/pt-br/assuntos/sanidade-animal-e-vegetal/saude-animal/programas-de-saude-animal/pncebt/principais-normas-pncebt/in-10-de-3-de-marco-de-2017-aprova-o-regulamento-tecnico-do-pncebt.pdf/view.pdf
Pereira, J. D. B., Cerqueira, V. D., Bezerra, P. S., Bezerra, D. K. O., Araújo, F. R., Dias, A. de C. L.,... Riet-Correa, G. (2017). Diagnóstico histopatológico e molecular de lesões sugestivas de tuberculose em búfalos abatidos nos municípios de macapá e santana, estado do amapá. Pesquisa Veterinária Brasileira, 37(11), 1198-1204. doi: 10.1590/s0100-736x2017001100003
Petroff, S. A. (1915). A new and rapid method for the isolation and cultivation of tubercle bacilli directly from the sputum and feces. Journal of Experimental Medicine, 21(1), 38-42. doi: 10.1084/jem.21.1.38
Ramos, J. M., Heinemann, M. B., Ferreira, J. S., Neto., Souza, A. F., Fº., Cárdenas, N. C., Dantas, A. F. S.,... Azevedo, S. S. (2016). Isolamento e identificação de Mycobacterium bovis em bovinos positivos no teste de tuberculinização no estado da Paraíba, nordeste do Brasil. Arquivos do Instituto Biológico, 85(e0842016), 1-7. doi: 10.1590/18081657000842016
Sales, M. L., Fonseca, A. A., Jr., Sales, É. B., Cottorello, A. C. P., Issa, M. A., Hodon, M. A.,... Heinemann, M. B. (2014). Evaluation of molecular markers for the diagnosis of Mycobacterium bovis. Folia Microbiologica, 59(5), 433-438. doi: 10.1007/s12223-014-0317-3
Secretaria de Planejamento do Rio Grande do Norte (2016). Diagnóstico e planejamento estratégico para o desenvolvimento de atividades produtivas agrícolas do Rio Grande do Norte. Recuperado de http:// www.rnsustentavel.rn.gov.br
Silva, M. R., Rocha, A. da S., Araújo, F. R., Fonseca, A. A., Jr., Alencar, A. P. de, Suffys, P. N.,... Guimarães, M. D. C. (2018). Risk factors for human Mycobacterium bovis infections in an urban area of Brazil. Memórias do Instituto Oswaldo Cruz, 113(8), 1-6. doi: 10.1590/0074-02760170445
Veloso, F. P., Baumgartem, K. D., Mota, A. L. A. D. A., Ferreira, F., Ferreira, J. S., Neto., Grisi, J. H., Fº.,... Gonçalves, V. S. P. (2016). Prevalence and herd-level risk factors of bovine tuberculosis in the State of Santa Catarina. Semina: Ciências Agrárias, 37(5), 3659-3672. doi: 10.5433/1679-0359.2016v37n5 Supl2p3659
Downloads
Published
2021-07-02
How to Cite
Bezerra Neto, P. S., Araújo, F. R. de, Pasquatti, T. N., Garino Junior, F., Medeiros, G. B., Firmino, M. de O., … Higino, S. S. dos S. (2021). Tuberculosis in bovine slaughtered in the semi-arid region of Rio Grande do Norte, Brazil. Semina: Ciências Agrárias, 42(5), 3047–3056. https://doi.org/10.5433/1679-0359.2021v42n5p3047
Issue
Section
Communication
License
Copyright (c) 2021 Semina: Ciências Agrárias
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Semina: Ciências Agrárias adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.
