Docosahexaenoic acid associated vitamin E in the diluent for cryopreservation of goat semen
DOI:
https://doi.org/10.5433/1679-0359.2021v42n1p255Keywords:
Computer assisted sperm analysis, Lipids, Omega 3, Plasma membrane, Spermatozoa.Abstract
The objective of this study was to evaluate the effect of and determine the optimum level of inclusion of docosahexaenoic acid (DHA) in the diluent for goat semen cryopreservation. Five Boer males underwent semen collection, totaling 10 viable collections per animal. After evaluation, the ejaculates were pooled and fractionated in Tris-yolk medium with the addition of 0; 30; 45; or 60ng mL-1 of DHA and 0.4 mmol of alpha-tocopherol (Vitamin E). The semen was cryopreserved in a freezing machine (TK 3000TM) and placed in a cryogenic cylinder for subsequent analysis. Data were evaluated by regression analysis at 5% significance. There were no differences (P > 0.05) in sperm kinetic parameters evaluated by computer assisted sperm analysis: total motility (79.17 ± 17.31%), progressive motility (14.04 ± 5.73%), curvilinear speed (58.82 ± 6.35µm/s), progressive linear speed (22.49 ± 3.63µm/s), mean path speed (35.17 ± 4.52µm/s), linearity (38.69 ± 5.79%), rectilinearity (63.99 ± 6.64%), and oscillation index (59.68 ± 2.99%). There were no differences (P > 0.05) found from the membrane functional integrity test for reactive spermatozoa (69.66 ± 9.76%), plasma and acrosomal membrane integrity of intact spermatozoa (29.86 ± 7.57%), mitochondrial potential of Class I cryopreserved goat semen (72.75 ± 9.81%), and chromatin compaction of intact chromatin (96.87 ± 4.37%). Thus, the inclusion of up to 60ng mL-1 of DHA did not promote any improvement in the seminal quality parameters of post-thawed goat semen.Downloads
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References
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Maia, M. S., & Bicudo, S. D. (2009). Radicais livres, antioxidantes e função espermática em mamíferos: uma revisão. Revista Brasileira de Reprodução Animal, 33(1), 183-193.
Maldjian, A., Pizzi, F., Gliozzi, T., Cerolini, S., Penny, P., & Noble, R. (2005). Changes in sperm quality and lipid composition during cryopreservation of boar semen. Theriogenology, 63(2), 411-421. doi: 10.1016/j. theriogenology.2004.09.021
Marco-Jiménez, F., Viudes-de-Castro, M. P., Balasch, S., Mocé, E., Silvestre, M. A., Gomez, E. A., & Vicente, J. S. (2006). Morphometric changes in goat sperm heads induced by cryopreservation. Cryobiology, 52(2), 295-304. doi: 10.1016/j.cryobiol. 2006.01.002
Nasiri, A. H., Towhidi, A., & Zeinoaldini, S. (2012). Combined effect of DHA and α-tocopherol supplementation during bull semen cryopreservation on sperm characteristics and fatty acid composition. Andrology, 44(1), 550-555. doi: 10.1111/j.1439-0272.2011.01225.x
SPSS Statistics (2015). Programa SPSS Statistics. Command Syntax Reference (versão 23.0). Chicago, IL: SPSS Inc.
Takahashi, T., Itoh, R., Nishinomiya, H., & Manabe, N. (2012). Effect of linoleic acid albumin in a diluition solution and long-term equilibration for freezing of bovine spermatozoa with poor freezability. Reproduction in Domestic Animal, 47(1), 92-97. doi: 10.1111/ j.1439-0531.2011.01806.x
Towhidi, A., Zeinoaldini, S., Ardebili, R., Davachi, N. D., & Nasiri, A. H. (2013). Combined n-3 fatty acids and α-Tocopherol supplementation improved the ovine sperm Cryosurvival. Iranian Journal of Biotechnology, 11(4), 238-43. doi: 10.5812/IJB.14469
Tsakmakidis, I. A. (2010). Ram semen evaluation: development and efficiency of modern techniques. Small Ruminant Research, 92(1-3), 126-130. doi: 10.1016/ j.smallrumres.2010.04.017
Aguiar, C. S., Barros, C. H. S. C., Machado, W. M., Allaman, I. B., Barbosa, L. P., & Snoeck, P. P. N. (2020). Efeito do ácido docosa-hexaenoico e do Trolox® no diluidor de refrigeração de sêmen de garanhões da raça Mangalarga Marchador. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 72(1), 71-78. doi: 10.1590/1678-4162-10823
Ansari, M., Towhidi, A., Shahrbabak, M. M., & Bahreini, M. (2012). Docosahexaenoic acid and alpha-tocopherol improve sperm cryosurvival in goat. Slovak Journal of Animal Science, 45(1), 7-13.
Apriokur, J. S. (2013). Pharmacology of free radicals and the impact of reactive oxygen species on the testis. Journal of Reproduction and Infertility, 14(4), 158-172.
Beletti, M. E., & Mello, M. L. S. (2004). Comparison between the toluidina blue stain and the Feulgen reaction for evaluation of rabbit sperm chromatin condensation and their relationship with sperm morphology. Theriogenology, 62(3-4), 398-402. doi: 10.1016/j.theriogenology.2003.10.016
Bucak, M. N., Sariözkan, S., Tuncer, P. B., Sakin, F., Ateşşahin, A., Kulaksız, R., & Cevik, M. (2010). The effect of antioxidants on post-thawed Angora goat (Capra hircus ancryrensis) spermparameters, lipid peroxidation and antioxidant activities. Small Ruminant Research, 89(1), 24-30. doi: 10.1016/j. smallrumres.2009.11.015
Cavalcante, T. V., Esper, C. R., & Ferreira, J. L. (2005). Avaliação da atividade mitocondrial em espermatozóides pós-colheita e pós-descongelação de caprinos das raças boer e alpina durante as estações reprodutiva e não reprodutiva. Archives of Veterinary Science, 10(2), 89-93. doi: 10.5380/avs.v10i2.4422
Colégio Brasileiro de Reprodução Animal (2013). Manual para exame andrológico e avaliação do sêmen animal (3a ed.). Belo Horizonte: CBRA.
Del Valle, I., Souter, A., & Maxwell, W. M. C. (2013). Function of ram spermatozoa frozen in diluents supplemented with casein and vegetable oils. Animal Reproduction Science, 138(1), 213-219. doi: 10. 1016/j.anireprosci.2013.02.022
Dorado, J., Hidalgo, M., Muñoz, M., & Rodríguez, I. (2009). Assessment of goat semen freezability according to the spermatozoa characteristics from fresh and frozen sample. Animal Reproduction Science, 112(1-2), 150-157. doi: 10.1016/j.anireprosci.2008.04.005
Flesh, F. M., & Gadella, B. M. (2000). Dynamics of the mammalian sperm plasma membrane in the process of fertilization. Biochimica et Biophysica Acta, 1469(3), 197-235. doi: 10.1016/s0304-4157(00)00018-6
González-Ravina, C., Aguirre-Lipperheide, M., Pinto, F., Martín-Lozano, D., Fernández-Sánchez, M., Blasco, V.,… Candenas, L. (2018). Effect of dietary supplementation with a highly pure and concentrated docosahexaenoic acid (DHA) supplement on human sperm function. Reproductive biology, 18(3), 282-288. doi: 10.1016/j.repbio.2018.06.002
Kaka, A., Haron, W., Yusoff, R., Yimer, N., Khumran, A. M., Memon, A. A.,… Ebrahimi, M. (2017). Frozen-thawed quality of bull semen after combined supplementation of docosahexaenoic acid and alpha linolenic acid into tris based semen extender. Pakistan Journal Zoological, 49(6), 2051-2055. doi: 10.17582/ journal.pjz/2017.49.6.2051.2055
Kaka, A., Wahid, H., Rosnina, Y., Khumran, A. M., Sarsaifi, K., Behan, A. A.,... Ebrahimi, M. (2015). α-Linolenicacid supplementation in BioXcell® extender can improve the quality of post-cooling and frozen-thawed bovine sperm. Animal Reproduction Science, 153(1), 1-7. doi: 10.1016/j.anireprosci.2014.12.001
Kamimura, C. F., Jacomini, J. O., & Beletti, M. E. (2010). Alterações de cromatina em 596 espermatozóides de ovinos e caprinos avaliadas por azul de toluidina e alaranjado 597 de acridina. Ciência Agrotecnica, 34(1), 212-219. doi: 10.1590/S1413-70542010000100027
Losano, J. D. A., Angrimani, D. S. R., Rui, B. R., Bicudo, L. C., Dalmazzo, A., Silva, B. C. S.,... Nichi, M. (2018). The addition of docosahexaenoic acid (DHA) and antioxidants (glutathione peroxidase and superoxide dismutase) in extenders to epididymal sperm cryopreservation in bulls. Zygote, 26(3), 199-206. doi: 10.1017/S0967199418000096
Machado, W. M., Barbosa, L. P., Souza, R. S., França, C. S., Pinheiro, E. E. G., Lents, M. P.,... Santana, A. L. A. (2018). Óleo de peixe associado ao ácido ascórbico no diluidor para criopreservação de sêmen caprino. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 70(1), 131-138. doi: 10.1590/1678-4162-9506
Maia, M. S., & Bicudo, S. D. (2009). Radicais livres, antioxidantes e função espermática em mamíferos: uma revisão. Revista Brasileira de Reprodução Animal, 33(1), 183-193.
Maldjian, A., Pizzi, F., Gliozzi, T., Cerolini, S., Penny, P., & Noble, R. (2005). Changes in sperm quality and lipid composition during cryopreservation of boar semen. Theriogenology, 63(2), 411-421. doi: 10.1016/j. theriogenology.2004.09.021
Marco-Jiménez, F., Viudes-de-Castro, M. P., Balasch, S., Mocé, E., Silvestre, M. A., Gomez, E. A., & Vicente, J. S. (2006). Morphometric changes in goat sperm heads induced by cryopreservation. Cryobiology, 52(2), 295-304. doi: 10.1016/j.cryobiol. 2006.01.002
Nasiri, A. H., Towhidi, A., & Zeinoaldini, S. (2012). Combined effect of DHA and α-tocopherol supplementation during bull semen cryopreservation on sperm characteristics and fatty acid composition. Andrology, 44(1), 550-555. doi: 10.1111/j.1439-0272.2011.01225.x
SPSS Statistics (2015). Programa SPSS Statistics. Command Syntax Reference (versão 23.0). Chicago, IL: SPSS Inc.
Takahashi, T., Itoh, R., Nishinomiya, H., & Manabe, N. (2012). Effect of linoleic acid albumin in a diluition solution and long-term equilibration for freezing of bovine spermatozoa with poor freezability. Reproduction in Domestic Animal, 47(1), 92-97. doi: 10.1111/ j.1439-0531.2011.01806.x
Towhidi, A., Zeinoaldini, S., Ardebili, R., Davachi, N. D., & Nasiri, A. H. (2013). Combined n-3 fatty acids and α-Tocopherol supplementation improved the ovine sperm Cryosurvival. Iranian Journal of Biotechnology, 11(4), 238-43. doi: 10.5812/IJB.14469
Tsakmakidis, I. A. (2010). Ram semen evaluation: development and efficiency of modern techniques. Small Ruminant Research, 92(1-3), 126-130. doi: 10.1016/ j.smallrumres.2010.04.017
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Published
2021-01-19
How to Cite
França, C. S. da, Bezerra, P. A., Mendes, C. S., Rocha, L. F., Santana, A. L. A., Souza, R. S., … Barbosa, L. P. (2021). Docosahexaenoic acid associated vitamin E in the diluent for cryopreservation of goat semen. Semina: Ciências Agrárias, 42(1), 255–266. https://doi.org/10.5433/1679-0359.2021v42n1p255
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