Low density lipoproteins added to an extender frozen or lyophilized are evenly efficient in cryoprotecting ovine sperm cells than when 16% whole egg yolk was added

Autores

  • Ana Maria Loaiza-Echeverri Universidade Federal de Minas Gerais, Escola de Veterinária
  • Betina Carla Cruz Universidade Federal de Minas Gerais, Escola de Veterinária
  • Paola Pereira das Neves Snoeck Universidade Estadual de Santa Cruz
  • Luís Cláudio Oliveira Moura Universidade Estadual de Santa Cruz
  • Beatriz Parzewski Neves Universidade Federal de Minas Gerais, Escola de Veterinária
  • Mariana Machado Neves Universidade Federal de Viçosa
  • Luiz Guilherme Dias Heneine Fundação Ezequiel Dias
  • Marc Henry Universidade Federal de Minas Gerais

DOI:

https://doi.org/10.5433/1679-0359.2015v36n3p1315

Palavras-chave:

Sperm cryopreservation, Low-density lipoproteins, Ovine.

Resumo

The aim of this study was to test different concentrations of low density lipoprotein (LDL) in replacement of whole egg yolk in extenders preserved in the aqueous or lyophilized form, for ram sperm cryopreservation using two cooling curves (-40°C/min from 5 to –140°C and nitrogen vapor). One ejaculate from six Santa Inês rams was collected. Each ejaculate was divided into nine different diluents as follows: Tris-16% yolk (control), and Tris with 2, 4, 6 and 8% fresh LDL, and criopreserved in the aqueous or lyophilized form. The samples were diluted to a final concentration of 100 x 106 sperm/mL and filled into 0.25 ml straws. After thaw, sperm cells were evaluated for motility and sperm kinetics (CASA), and submitted to the hypoosmotic swelling test and the evaluation of the structural integrity of sperm membranes using fluorescent dyes (CFDA: PI), as well as sperm morphology and longevity. The experimental design was randomized blocks, and results were submitted to ANOVA and the averages were compared using the Scott-Knott test. There were no differences in progressive motility and functional and structural integrity of the membrane evaluated when different concentrations of aqueous or lyophilized low density lipoproteins or egg yolk were added to the extender (P>0.05). As for the velocity of sperm movement, the control medium had some kinetic scores similar to the extender containing LDL, both aqueous and lyophilized (P> 0.05). Results were similar between cooling curves. Therefore, we conclude that the media containing all concentrations of LDL, aqueous or lyophilized, were able to protect the ram sperm cells during the cryopreservation process, as whole egg yolk did.

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Biografia do Autor

Ana Maria Loaiza-Echeverri, Universidade Federal de Minas Gerais, Escola de Veterinária

Pesquisadora, Escola de Veterinária da Universidade Federal de Minas Gerais, UFMG, Belo Horizonte, MG,, Brasil.

Betina Carla Cruz, Universidade Federal de Minas Gerais, Escola de Veterinária

Discente, UFMG, Belo Horizonte, MG, Brasil.

Paola Pereira das Neves Snoeck, Universidade Estadual de Santa Cruz

Profª, Escola de Veterinária, Universidade Estadual de Santa Cruz, UESC, Ilheus, BA, Brasil.

Luís Cláudio Oliveira Moura, Universidade Estadual de Santa Cruz

 Discente, Escola de Veterinária, UESC, Ilheus, BA, Brasil.

Beatriz Parzewski Neves, Universidade Federal de Minas Gerais, Escola de Veterinária

Discente, UFMG, Belo Horizonte, MG, Brasil.

Mariana Machado Neves, Universidade Federal de Viçosa

Profª, Deptº de Biologia Geral, Universidade Federal de Viçosa, UFV, Viçosa, MG, Brasil.

Luiz Guilherme Dias Heneine, Fundação Ezequiel Dias

Pesquisador, Laboratório de Imunologia, Fundação Ezequiel Dias, FUNED, Belo Horizonte, MG, Brasil.

 

Marc Henry, Universidade Federal de Minas Gerais

Prof., UFMG, Belo Horizonte, MG, Brasil.

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Publicado

2015-06-10

Como Citar

Loaiza-Echeverri, A. M., Cruz, B. C., Snoeck, P. P. das N., Moura, L. C. O., Neves, B. P., Neves, M. M., Heneine, L. G. D., & Henry, M. (2015). Low density lipoproteins added to an extender frozen or lyophilized are evenly efficient in cryoprotecting ovine sperm cells than when 16% whole egg yolk was added. Semina: Ciências Agrárias, 36(3), 1315–1346. https://doi.org/10.5433/1679-0359.2015v36n3p1315

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