Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat?
DOI:
https://doi.org/10.5433/1679-0359.2019v40n6Supl3p3789Keywords:
COCs, Bovine oocytes, In vitro maturation, Ex-situ maturation, Nonsurgical embryo transfer technique, Goat.Abstract
We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student’s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat’s uterus could present an alternative to nuclear maturation.References
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SPRÍCIGO, J. F.; SENA NETTO, S. B.; MUTERLLE, C. V.; RODRIGUES, S. A.; LEME, L. O.; GUIMARÃES, A. L.; CAIXETA, F. M.; FRANCO, M. M.; PIVATO, I.; DODE, M. A. Intrafollicular transfer of fresh and vitrified immature bovine oocytes. Theriogenology, Amsterdam, v. 86, n. 8, p. 2054-2062, 2016. DOI: 10.1016/j.theriogenology.2016.07.003.
VAJTA, G.; RINDOM, N.; PEURA, T. T.; HOLM, P.; GREVE, T.; CALLESEN, H. The effect of media, serum and temperature on in vitro survival of bovine blastocysts after open pulled straw (OPS) vitrification. Theriogenology, Amsterdam, v. 52, n. 5, p. 939-948, 1999. DOI: 10.1016/S0093-691X(99)00184-3.
VIANA, J. H. M.; FIGUEIREDO, A. C. S.; SIQUEIRA, L. G. B. Brazilian embryo industry in context: pitfalls, lessons, and expectations for the future. Animal Reproduction, Belo Horizonte, v. 14, n. 3, p. 476-481, 2017. DOI: 10.21451/1984-3143-AR989.
FAIR, T.; LONERGAN, P.; DINNYES, A.; COTTELL, D. C.; HYTTEL, P.; WARD, F. A.; BOLAND, M. P. Ultrastructure of bovine blastocysts following cryopreservation: effect of method of blastocyst production. Molecular Reproduction and Development, New York, v. 58, n. 2, p. 186-195, 2001. DOI: 10.1002/1098-2795(200102)58:2 186::AID-MRD83.0.CO;2-N
FONSECA, J. F.; ESTEVES, L. V.; ZAMBRINI, F. N.; BRANDÃO, F. Z.; PEIXOTO, M. G. C. D.; VERNEQUE, R. S.; SIQUEIRA, L. G. B.; VIANA, J. H. M. Viable offspring after successful nonsurgical embryo transfer in goats. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 66, n. 2, p. 613-616, 2014. DOI: http://dx.doi.org/10.1590/1678-41626783
FONSECA, J. F.; SOUZA-FABJAN, J. M. G.; OLIVEIRA, M. E. F.; LEITE, C. R.; NASCIMENTO-PENIDO, P. M. P.; BRANDÃO, F. Z.; LEHLOENYA, K. C. Nonsurgical embryo recovery and transfer in sheep and goats. Theriogenology, Amsterdam, v. 86, n. 1, p. 144-151, 2016. DOI: 10.1016/j.theriogenology.2016.04.025.
FONSECA, J. F.; ZAMBRINI, F. N.; ALVIM, G. P.; PEIXOTO, M. G. C. D.; VERNEQUE, R. S.; VIANA, J. H. M. Embryo production and recovery in goats by non-surgical transcervical technique. Small Ruminant Research, Amsterdam, v. 111, n. 1-3, p. 96-99, 2013. DOI: 10.1016/j.smallrumres.2012.08.007
FUKUI, Y.; FUKUSHIMA, M.; ONO, H. Fertilization and cleavage of bovine follicular oocytes in rabbit reproductive tracts after maturation in vitro. Journal of Experimental Zoology, New York, v. 226, n. 1, p. 137-142, 1983. DOI: 10.1002/jez.1402260116.
LEROY, J. L.; GENICOT, G.; DONNAY, I.; VAN SOOM, A. Evaluation of the lipid content in bovine oocytes and embryos with nile red: a practical approach. Reproduction in Domestic Animals, Berlin, v. 40, n. 1, p. 76-78, 2005. DOI: 10.1111/j.1439-0531.2004.00556.x.
POLGE, C.; WILMUT, J.; ROWSON, L. E. A. The low temperature preservation of cow, sheep and pig embryos. Cryobiology, San Diego, v. 11, n. 6, p. 560, 1974. DOI: 10.1016/0011-2240(74)90195-3.
PRATES, E. G.; NUNES, J. T.; PEREIRA, R. M. A role of lipid metabolism during cumulus-oocyte complex maturation: impact of lipid modulators to improve embryo production. Mediators of Inflammation., Sylvania, v. Article ID 692067, p. 1-11, 2014. DOI: 10.1155/2014/692067.
RIZOS, D.; WARD, F.; DUFFY, P.; BOLAND, M. P.; LONERGAN, P. Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: implications for blastocyst yield and blastocyst quality. Molecular Reproduction and Development, New York, v. 61, n. 2, p. 234-248, 2002. DOI: 10.1002/mrd.1153.
SIRARD, M. A.; RICHARD, F.; BLONDIN, P.; ROBERT, C. Contribution of the oocyte to embryo quality. Theriogenology, Amsterdam, v. 7, n. 1, p. 126-136, 2006. DOI: 10.1016/j.theriogenology.2005.09.020.
SPRÍCIGO, J. F.; SENA NETTO, S. B.; MUTERLLE, C. V.; RODRIGUES, S. A.; LEME, L. O.; GUIMARÃES, A. L.; CAIXETA, F. M.; FRANCO, M. M.; PIVATO, I.; DODE, M. A. Intrafollicular transfer of fresh and vitrified immature bovine oocytes. Theriogenology, Amsterdam, v. 86, n. 8, p. 2054-2062, 2016. DOI: 10.1016/j.theriogenology.2016.07.003.
VAJTA, G.; RINDOM, N.; PEURA, T. T.; HOLM, P.; GREVE, T.; CALLESEN, H. The effect of media, serum and temperature on in vitro survival of bovine blastocysts after open pulled straw (OPS) vitrification. Theriogenology, Amsterdam, v. 52, n. 5, p. 939-948, 1999. DOI: 10.1016/S0093-691X(99)00184-3.
VIANA, J. H. M.; FIGUEIREDO, A. C. S.; SIQUEIRA, L. G. B. Brazilian embryo industry in context: pitfalls, lessons, and expectations for the future. Animal Reproduction, Belo Horizonte, v. 14, n. 3, p. 476-481, 2017. DOI: 10.21451/1984-3143-AR989.
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2019-10-16
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Fonseca, J. F. da, Batista, R. I. T. P., Trevizan, J. T., Souza-Fabjan, J. M. G., Brandão, F. Z., & Camargo, L. S. A. (2019). Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat?. Semina: Ciências Agrárias, 40(6Supl3), 3789–3796. https://doi.org/10.5433/1679-0359.2019v40n6Supl3p3789
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