Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat?
DOI:
https://doi.org/10.5433/1679-0359.2019v40n6Supl3p3789Keywords:
COCs, Bovine oocytes, In vitro maturation, Ex-situ maturation, Nonsurgical embryo transfer technique, Goat.Abstract
We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student’s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat’s uterus could present an alternative to nuclear maturation.Downloads
Download data is not yet available.
References
ENRIGHT, B. P.; LONERGAN, P.; DINNYES, A.; FAIR, T.; WARD, F. A.; YANG, X.; BOLAND, M. P. Culture of in vitro produced bovine zygotes in vitro versus in vivo: implications for early embryo development and quality. Theriogenology, Amsterdam, v. 54, n. 5, p. 659-673, 2000. DOI: 10.1016/S0093-691X(00)00381-2
FAIR, T.; LONERGAN, P.; DINNYES, A.; COTTELL, D. C.; HYTTEL, P.; WARD, F. A.; BOLAND, M. P. Ultrastructure of bovine blastocysts following cryopreservation: effect of method of blastocyst production. Molecular Reproduction and Development, New York, v. 58, n. 2, p. 186-195, 2001. DOI: 10.1002/1098-2795(200102)58:2 186::AID-MRD83.0.CO;2-N
FONSECA, J. F.; ESTEVES, L. V.; ZAMBRINI, F. N.; BRANDÃO, F. Z.; PEIXOTO, M. G. C. D.; VERNEQUE, R. S.; SIQUEIRA, L. G. B.; VIANA, J. H. M. Viable offspring after successful nonsurgical embryo transfer in goats. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 66, n. 2, p. 613-616, 2014. DOI: http://dx.doi.org/10.1590/1678-41626783
FONSECA, J. F.; SOUZA-FABJAN, J. M. G.; OLIVEIRA, M. E. F.; LEITE, C. R.; NASCIMENTO-PENIDO, P. M. P.; BRANDÃO, F. Z.; LEHLOENYA, K. C. Nonsurgical embryo recovery and transfer in sheep and goats. Theriogenology, Amsterdam, v. 86, n. 1, p. 144-151, 2016. DOI: 10.1016/j.theriogenology.2016.04.025.
FONSECA, J. F.; ZAMBRINI, F. N.; ALVIM, G. P.; PEIXOTO, M. G. C. D.; VERNEQUE, R. S.; VIANA, J. H. M. Embryo production and recovery in goats by non-surgical transcervical technique. Small Ruminant Research, Amsterdam, v. 111, n. 1-3, p. 96-99, 2013. DOI: 10.1016/j.smallrumres.2012.08.007
FUKUI, Y.; FUKUSHIMA, M.; ONO, H. Fertilization and cleavage of bovine follicular oocytes in rabbit reproductive tracts after maturation in vitro. Journal of Experimental Zoology, New York, v. 226, n. 1, p. 137-142, 1983. DOI: 10.1002/jez.1402260116.
LEROY, J. L.; GENICOT, G.; DONNAY, I.; VAN SOOM, A. Evaluation of the lipid content in bovine oocytes and embryos with nile red: a practical approach. Reproduction in Domestic Animals, Berlin, v. 40, n. 1, p. 76-78, 2005. DOI: 10.1111/j.1439-0531.2004.00556.x.
POLGE, C.; WILMUT, J.; ROWSON, L. E. A. The low temperature preservation of cow, sheep and pig embryos. Cryobiology, San Diego, v. 11, n. 6, p. 560, 1974. DOI: 10.1016/0011-2240(74)90195-3.
PRATES, E. G.; NUNES, J. T.; PEREIRA, R. M. A role of lipid metabolism during cumulus-oocyte complex maturation: impact of lipid modulators to improve embryo production. Mediators of Inflammation., Sylvania, v. Article ID 692067, p. 1-11, 2014. DOI: 10.1155/2014/692067.
RIZOS, D.; WARD, F.; DUFFY, P.; BOLAND, M. P.; LONERGAN, P. Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: implications for blastocyst yield and blastocyst quality. Molecular Reproduction and Development, New York, v. 61, n. 2, p. 234-248, 2002. DOI: 10.1002/mrd.1153.
SIRARD, M. A.; RICHARD, F.; BLONDIN, P.; ROBERT, C. Contribution of the oocyte to embryo quality. Theriogenology, Amsterdam, v. 7, n. 1, p. 126-136, 2006. DOI: 10.1016/j.theriogenology.2005.09.020.
SPRÍCIGO, J. F.; SENA NETTO, S. B.; MUTERLLE, C. V.; RODRIGUES, S. A.; LEME, L. O.; GUIMARÃES, A. L.; CAIXETA, F. M.; FRANCO, M. M.; PIVATO, I.; DODE, M. A. Intrafollicular transfer of fresh and vitrified immature bovine oocytes. Theriogenology, Amsterdam, v. 86, n. 8, p. 2054-2062, 2016. DOI: 10.1016/j.theriogenology.2016.07.003.
VAJTA, G.; RINDOM, N.; PEURA, T. T.; HOLM, P.; GREVE, T.; CALLESEN, H. The effect of media, serum and temperature on in vitro survival of bovine blastocysts after open pulled straw (OPS) vitrification. Theriogenology, Amsterdam, v. 52, n. 5, p. 939-948, 1999. DOI: 10.1016/S0093-691X(99)00184-3.
VIANA, J. H. M.; FIGUEIREDO, A. C. S.; SIQUEIRA, L. G. B. Brazilian embryo industry in context: pitfalls, lessons, and expectations for the future. Animal Reproduction, Belo Horizonte, v. 14, n. 3, p. 476-481, 2017. DOI: 10.21451/1984-3143-AR989.
FAIR, T.; LONERGAN, P.; DINNYES, A.; COTTELL, D. C.; HYTTEL, P.; WARD, F. A.; BOLAND, M. P. Ultrastructure of bovine blastocysts following cryopreservation: effect of method of blastocyst production. Molecular Reproduction and Development, New York, v. 58, n. 2, p. 186-195, 2001. DOI: 10.1002/1098-2795(200102)58:2 186::AID-MRD83.0.CO;2-N
FONSECA, J. F.; ESTEVES, L. V.; ZAMBRINI, F. N.; BRANDÃO, F. Z.; PEIXOTO, M. G. C. D.; VERNEQUE, R. S.; SIQUEIRA, L. G. B.; VIANA, J. H. M. Viable offspring after successful nonsurgical embryo transfer in goats. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 66, n. 2, p. 613-616, 2014. DOI: http://dx.doi.org/10.1590/1678-41626783
FONSECA, J. F.; SOUZA-FABJAN, J. M. G.; OLIVEIRA, M. E. F.; LEITE, C. R.; NASCIMENTO-PENIDO, P. M. P.; BRANDÃO, F. Z.; LEHLOENYA, K. C. Nonsurgical embryo recovery and transfer in sheep and goats. Theriogenology, Amsterdam, v. 86, n. 1, p. 144-151, 2016. DOI: 10.1016/j.theriogenology.2016.04.025.
FONSECA, J. F.; ZAMBRINI, F. N.; ALVIM, G. P.; PEIXOTO, M. G. C. D.; VERNEQUE, R. S.; VIANA, J. H. M. Embryo production and recovery in goats by non-surgical transcervical technique. Small Ruminant Research, Amsterdam, v. 111, n. 1-3, p. 96-99, 2013. DOI: 10.1016/j.smallrumres.2012.08.007
FUKUI, Y.; FUKUSHIMA, M.; ONO, H. Fertilization and cleavage of bovine follicular oocytes in rabbit reproductive tracts after maturation in vitro. Journal of Experimental Zoology, New York, v. 226, n. 1, p. 137-142, 1983. DOI: 10.1002/jez.1402260116.
LEROY, J. L.; GENICOT, G.; DONNAY, I.; VAN SOOM, A. Evaluation of the lipid content in bovine oocytes and embryos with nile red: a practical approach. Reproduction in Domestic Animals, Berlin, v. 40, n. 1, p. 76-78, 2005. DOI: 10.1111/j.1439-0531.2004.00556.x.
POLGE, C.; WILMUT, J.; ROWSON, L. E. A. The low temperature preservation of cow, sheep and pig embryos. Cryobiology, San Diego, v. 11, n. 6, p. 560, 1974. DOI: 10.1016/0011-2240(74)90195-3.
PRATES, E. G.; NUNES, J. T.; PEREIRA, R. M. A role of lipid metabolism during cumulus-oocyte complex maturation: impact of lipid modulators to improve embryo production. Mediators of Inflammation., Sylvania, v. Article ID 692067, p. 1-11, 2014. DOI: 10.1155/2014/692067.
RIZOS, D.; WARD, F.; DUFFY, P.; BOLAND, M. P.; LONERGAN, P. Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: implications for blastocyst yield and blastocyst quality. Molecular Reproduction and Development, New York, v. 61, n. 2, p. 234-248, 2002. DOI: 10.1002/mrd.1153.
SIRARD, M. A.; RICHARD, F.; BLONDIN, P.; ROBERT, C. Contribution of the oocyte to embryo quality. Theriogenology, Amsterdam, v. 7, n. 1, p. 126-136, 2006. DOI: 10.1016/j.theriogenology.2005.09.020.
SPRÍCIGO, J. F.; SENA NETTO, S. B.; MUTERLLE, C. V.; RODRIGUES, S. A.; LEME, L. O.; GUIMARÃES, A. L.; CAIXETA, F. M.; FRANCO, M. M.; PIVATO, I.; DODE, M. A. Intrafollicular transfer of fresh and vitrified immature bovine oocytes. Theriogenology, Amsterdam, v. 86, n. 8, p. 2054-2062, 2016. DOI: 10.1016/j.theriogenology.2016.07.003.
VAJTA, G.; RINDOM, N.; PEURA, T. T.; HOLM, P.; GREVE, T.; CALLESEN, H. The effect of media, serum and temperature on in vitro survival of bovine blastocysts after open pulled straw (OPS) vitrification. Theriogenology, Amsterdam, v. 52, n. 5, p. 939-948, 1999. DOI: 10.1016/S0093-691X(99)00184-3.
VIANA, J. H. M.; FIGUEIREDO, A. C. S.; SIQUEIRA, L. G. B. Brazilian embryo industry in context: pitfalls, lessons, and expectations for the future. Animal Reproduction, Belo Horizonte, v. 14, n. 3, p. 476-481, 2017. DOI: 10.21451/1984-3143-AR989.
Downloads
Published
2019-10-16
How to Cite
Fonseca, J. F. da, Batista, R. I. T. P., Trevizan, J. T., Souza-Fabjan, J. M. G., Brandão, F. Z., & Camargo, L. S. A. (2019). Goat incubator: Can bovine oocytes be matured in the uterine horn of a goat?. Semina: Ciências Agrárias, 40(6Supl3), 3789–3796. https://doi.org/10.5433/1679-0359.2019v40n6Supl3p3789
Issue
Section
Case Reports
License
Copyright (c) 2019 Semina: Ciências Agrárias
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Semina: Ciências Agrárias adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.
