Genetic diversity and paternity of Brycon orbignyanus offspring obtained for different reproductive systems

Authors

  • Nelson Mauricio Lopera-Barrero Universidade Estadual de Londrina
  • Camilo Andres Reyes Alvarez Universidad Nacional de La Plata
  • Maria del Pilar Rodriguez-Rodriguez Universidade Estadual de Maringá
  • Jayme Aparecido Povh Universidade Federal de Mato Grosso do Sul
  • Lauro Vargas Universidade Estadual de Maringá
  • Danilo Pedro Streit Júnior Universidade Federal do Rio Grande do Sul
  • Rodolfo Nardes Sirol Companhia Paulista de Força e Luz
  • Ricardo Pereira Ribeiro Universidade Estadual de Maringá

DOI:

https://doi.org/10.5433/1679-0359.2014v35n1p541

Keywords:

Extrusion system, Genetic conservation, Microsatellite, Piracanjuba, Restocking program, Semi-natural system.

Abstract

The objective of this study was to estimate the genetic diversity and the paternity of Brycon orbignyanus offspring’s obtained with the extrusion and semi-natural reproductive systems, by microsatellites markers. The four loci used produced 11 alleles, being observed three alleles (BoM1, BoM5 and BoM7) and two alleles (BoM2) for locus present in the parental and in the offspring of both reproductive systems. In the offspring of the extrusion system low frequency alleles was observed for the locus BoM5 (allele B = 0.095) and BoM7 (allele C = 0.059) and there was a decrease of genetic variability (observe heterozygosity-Ho = 0.900 and 0.823; Shannon index-IS = 0.937 and 0.886; Nei genetic diversity-DGN = 0.604 and 0.566, respectively). For the offspring of the semi-natural system the allele frequencies stayed stable being verified an unequal frequency for each locus. The genetic variability in the offspring was preserved, being corroborated by the Ho values (0.975 and 0.945), IS (0.927 and 0.924) and DGN (0.593 and 0.581) for parental and offspring, respectively. Deviations were observed (P <0.01) in the Hardy-Weinberg equilibrium and linkage disequilibrium for the two reproductive systems. The inbreeding coefficient (Fis) it showed deficit of heterozygote in the offspring of the extrusion system. Multiple paternity and differed reproductive contributions in the composition of the families in the offspring in the two reproductive systems was observed, with the presence of reproductive dominance in the semi-natural system.

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Author Biographies

Nelson Mauricio Lopera-Barrero, Universidade Estadual de Londrina

Prof. Dr. em Zootecnia, Deptº de Zootecnia, Universidade Estadual de Londrina, UEL, Londrina, PR.

Camilo Andres Reyes Alvarez, Universidad Nacional de La Plata

Biólogo, Mestrando em Tecnologia e Higiene de Alimentos, Universidad Nacional de La Plata, Av. 7, nº 877, La Plata, Buenos Aires, Argentina.

Maria del Pilar Rodriguez-Rodriguez, Universidade Estadual de Maringá

M.e em Zootecnia, Deptº de Zootecnia, Universidade Estadual de Maringá, UEM, Maringá, PR.

Jayme Aparecido Povh, Universidade Federal de Mato Grosso do Sul

Prof. Dr. em Zootecnia, Universidade Federal do Mato Grosso do Sul, Faculdade de Medicina Veterinária e Zootecnia, Campo Grande, MS.

Lauro Vargas, Universidade Estadual de Maringá

Prof. Dr. em Zootecnia, Deptº de Zootecnia UEM, Maringá, PR.

Danilo Pedro Streit Júnior, Universidade Federal do Rio Grande do Sul

Prof. Dr. em Zootecnia, Deptº de Zootecnia Universidade Federal do Rio Grande do Sul, UFRGS, Porto Alegre, RS.

Rodolfo Nardes Sirol, Companhia Paulista de Força e Luz

Pesquisador Dr. em Zootecnia, Companhia Paulista de Força e Luz, CPFL, Campinas, SP.

Ricardo Pereira Ribeiro, Universidade Estadual de Maringá

Prof. Dr. em Zootecnia, Deptº de Zootecnia UEM, Maringá, PR.

Published

2014-02-27

How to Cite

Lopera-Barrero, N. M., Alvarez, C. A. R., Rodriguez-Rodriguez, M. del P., Povh, J. A., Vargas, L., Streit Júnior, D. P., … Ribeiro, R. P. (2014). Genetic diversity and paternity of Brycon orbignyanus offspring obtained for different reproductive systems. Semina: Ciências Agrárias, 35(1), 541–554. https://doi.org/10.5433/1679-0359.2014v35n1p541

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