Susceptibility of the Asian citrus psyllid Diaphorina citri to entomopathogenic nematodes
DOI:
https://doi.org/10.5433/1679-0359.2024v45n6p2033Keywords:
Biological control, HLB, Greening, Asian citrus psyllid, Heterorhabditis, Steinernema.Abstract
The Asian citrus psyllid, Diaphorina citri Kuwayama, 1908, (Hemiptera: Psylloidea: Liviidae), is one of the most important pests for citrus production, as it is the main vector of ‘Candidatus Liberibacter spp.’ bacteria, the causal agents of Huanglongbing (HLB). Applications of chemical insecticides are currently the primary method for psyllid control, but have shown low efficiency and problems such as the development of resistant insect populations, and increased risks for the human health and the environment. Thus, the aim of this study was to assess the use of entomopathogenic nematodes (EPNs) as an alternative and sustainable strategy for the control of D. citri. Initially, the activity of 10 EPN isolates of the genera Heterorhabditis and Steinernema was tested against D. citri. Assessments were carried out on D. citri adults under laboratory conditions. Next, the efficiency of the concentrations of 50, 100, 150, and 200 Infective Juveniles (IJs)/cm² of the UENP 06 and NEPET 11 EPN isolates in causing the death of D. citri adults was tested. The NEPET 11 and UENP 06 isolates, both Heterorhabditis amazonensis, were the most effective in controlling psyllids, achieving confirmed mortality rates of 68.25 and 53.00%, respectively. In all IJ concentrations tested, these isolates caused confirmed mortality of D. citri adults, with no significant differences between concentrations and EPN isolates.
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Bassanezi, R. B., Lopes, S. A., Belasque Júnior, J., Spósito, M. B., Yamamoto, P. T., Miranda, M. P., Teixeira, D. C., & Wulff, N. A. (2010). Huanglongbing epidemiology and implications for disease management. Citrus Research & Technology, 31(1), 11-23. doi: 10.5935/2236-3122.20100002 DOI: https://doi.org/10.5935/2236-3122.20100002
Bové, J. M. (2006). Huanglongbing: a destructive, newly-emerging, century-old disease of citrus. Journal of Plant Pathology, 88(1), 7-37. doi: 10.4454/jpp.v88i1.828
Ciche, T. A., & Ensign, J. C. (2003). For the insect pathogen Photorhabdus luminescens, which of a nematode is out. Applied and Environmental Microbiology, 69(4), 1890-1897. doi: 10.1128/AEM.69.4.1890-1897.2003 DOI: https://doi.org/10.1128/AEM.69.4.1890-1897.2003
Companhia Nacional de Abastecimento (2022). Citricultura. CONAB. https://www.conab.gov.br/#
Dala-Paula, B. M., Plotto, A., Bai, J., Manthey, J. A., Baldwin, E. A., Ferrarezi, R. S., & Gloria, M. B. A. (2019). Effect of Huanglongbing or greening disease on orange juice quality, a review. Frontiers in Plant Science, 9, 1976. doi. 10.3389/fpls.2018.01976 DOI: https://doi.org/10.3389/fpls.2018.01976
Fernandes, T. A. P., Marcomini, M. C., Ferreira, F. P., Guide, B. A., Alves, V. S., & Neves, P. M. O. J. (2021). Native isolates and the effect of aviary litter on the pathogenicity and virulence of entomopathogenic nematodes for the control of the lesser meal worm, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae). Semina: Ciências Agrárias, 42(1), 1-18. doi: 10.5433/1679-0359.2021v42n1p1 DOI: https://doi.org/10.5433/1679-0359.2021v42n1p1
Ferreira, D. F. (2011). Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia, 35(6), 1039-1042. doi: 10.1590/S1413-70542011000600001 DOI: https://doi.org/10.1590/S1413-70542011000600001
Food Agricultural Organization (2019). Crops and livestock products. http://www.fao.org/faostat/en/#data/QCL/visualize
Giometti, F. H. C., Leite, L. G., Tavares, F. M., Schmit, F. S., Batista, A., Fº., & Dell'Acqua, R. (2011). Virulência de nematóides entomopatogênicos (Nematoda: Rhabditida) a Sphenophorus levis (Coleoptera: Curculionidae). Bragantia, 70(1), 81-86. doi: 10.1590/S0006-87052011000100013 DOI: https://doi.org/10.1590/S0006-87052011000100013
Grewal, P. S., Nardo E. B., & Aguillera M. M. (2001). Nematoides entomopatogênicos: potencial para exploração e uso na América do Sul. Neotropical Entomology, 30(2), 191-205. doi: 10.1590/S1519-566X2001000200001 DOI: https://doi.org/10.1590/S1519-566X2001000200001
Magnabosco, M. E. B., Andaló, V., & Carvalho, F. J. (2020). Susceptibility of Elasmopalpus lignosellus pupae to entomopathogenic nematodes in maize. Revista Brasileira de Milho e Sorgo, 19, e1115. doi: 10.18512/1980-6477/rbms 2020.v19.e1115 DOI: https://doi.org/10.18512/rbms2020v19e1115
Silva, M. A. T. da. (2011). Controle de Quesada gigas (Hemiptera: Cicadidae) pela aplicação de nematóides entomopatogênicos e compatibilidade com alguns produtos fitossanitários em cafeeiro. Tese de doutorado, Universidade Federal de Lavras, Lavras, MG, Brasil.
Tiwari, S., Mann, R. S., Rogers, M. E., & Stelinski, L. L. (2011). Insecticide resistance in field populations of Asian citrus psyllid in Florida. Pest Management Science, 67(10), 1258-1268. doi: 10.1002/ps.2181 DOI: https://doi.org/10.1002/ps.2181
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