Identification of antibodies to Leptospira spp. in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity
DOI:
https://doi.org/10.5433/1679-0359.2019v40n6Supl3p3763Keywords:
Leptospirosis, Microscopic agglutination test, Wild animal.Abstract
Leptospirosis is a zoonosis of worldwide distribution in which the agent can infect several animal species and accidentally humans. In view of the limited number of studies on anti-Leptospira antibodies in wild animal species, especially those living in aquatic environments, we sought in this study to investigate the presence of these antibodies in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity in the Rodrigues Alves Botanical Garden–Amazon Zoobotanical Park, located in Belém, Pará State, Brazil. Serum samples were collected from 31 turtles, and identification of anti-Leptospira antibodies was performed using the microscopic agglutination test, using a collection of 31 live antigens which represent 19 serogroups of Leptospira. Among the analyzed samples, 54.83% (17/31) were observed to be reactive, and co-agglutination was detected in a further six samples which were not accounted for in the frequency of serogroups. The most frequently detected serogroups were Tarassovi 72.72% (8/11), Celledoni 18.18% (2/11), and Pomona 9.09% (1/11)], with titers ranging from 200 to 400, being this the first study to report infection of these serogroups in this species of chelonios. The animals were kept in water tanks, which probably favored the transport of the agent and allowed its transmission to the spot-legged turtle. We thus confirmed presence of anti-Leptospira antibodies in chelonians maintained in the Rodrigues Alves Botanical Garden.Downloads
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References
ACHA, P. N.; SZYFRES, B. Zoonosis y enfermedades transmisibles comunes al hombre ya los animales. Washigton: Organización Panamericana de la Salud, 2003. v. 1, 398 p.
ANDREWS, R. D.; REILLY, J. R.; FERRIS, D.; HANSON, L. E. Leptospiral agglutinins in sera from Southern Illinois Herpetofauna. Journal of Wildlife Diseases, University of Illinois at Urbana-Champaign, v. 1, n.4, p. 55-59, 1965. DOI: 10.7589/0090-3558-1.4.55
CARDOSO, S. L. C. Tomada de decisão em Jardim Botânico: Bosque Rodrigues Alves-Jardim Zoobotânico da Amazônia (BRAJZBA). Amazônia, Organizações e Sustentabilidade, Belém, v. 6, n. 2, p. 123-131, 2018. DOI: http://dx.doi.org/10.17800/2238-8893/aos.v6n2jul/dez2017p123-131
DEZZUTTO, D.; BARBERO, R.; CANALE, L.; ACUTIS, P. L.; BIOLATTI, C.; DOGLIERO, A.; MITZY, M. D.; FRANCONE, P.; COLZANI, A.; BERGAGNA, S.; GENNERO, M. S. Detection of Leptospira spp. in water turtle (Trachemys scripta) living in ponds of urban parks. Veterinary Sciences, Basel, v. 4, n. 4, p. 51-55, 2017. DOI: 10.3390/vetsci4040051
EBANI, V. V. Domestic reptiles as source of zoonotic bacteria: a mini review. Asian Pacific Journal of Tropical Medicine, Haikou, v. 10, n. 8, p. 723-728, 2017. DOI: 10.1016/j.apjtm.2017.07.020
ESTEVES, F. M.; GUERRA-NETO, G.; GIRIO, R. J. S.; SILVA-VERGARA, M. L.; CARVALHO, A. C. F. B. Detecção de anticorpos para Leptospira spp. em animais e funcionários do zoológico municipal de Uberaba, MG. Arquivos do Instituto Biológico, São Paulo, v. 72, n. 3, p. 283-288, 2005.
FIGUEIREDO, M. W. Estrutura populacional, uso de ambientes e crescimento corporal de Rhinoclemmys punctularia punctularia (Daudin, 1801), na Ilha de Algodoal/ Maiandeua, Maracanã, Pará, Brasil. 2010. Dissertação (Mestrado) - Instituto de Ciências Biológicas, Universidade Federal do Pará, Pará.
FRETEY, J.; BILLES, A.; TIWARI, M. Leatherback, Dermochelys coriacea, nesting along the Atlantic coast of Africa. Chelonian Conservation Biology, v. 6, n. 1, p. 126-129, 2007. DOI: 10.2744/1071-8443
GLOSSER, J. W.; SULZER, C. R.; EBERHARDT, M.; WINKLER, W. G. Cultural and serologic evidence of Leptospira interrogans serotype Tarassovi infection in turtles. Journal of Wildlife. Diseases, Massachusetts, v. 10, n. 4, p. 429-435, 1974. DOI: 10.7589/0090-3558-10.4.429
GRIMM, K.; MITCHELL, M. A.; THOMPSON, D.; MADDOX, C. Seroprevalence of Leptospira spp. in Blanding's Turtles (Emydoidea blandingii) from DuPage County, Illinois USA. Journal of Herpetological Medicine and Surgery, Gainesville, v. 25, n. 1-2, p. 28-32, 2015. DOI: 10.5818/1529-9651-25.1.28
KIKTENKO, V.S.; ANANYIN, V. Leptospiroz na daljnem vostoke (leptospirose do Extremo Oriente). Zeitschrift für allgemeine Mikrobiologie, Moscou, n. 12, p. 72-78, 1941.
LINDTNER-KNIFIC, R.; VERGLES-RATAJ, A.; VLAHOVIĆ, K.; ZRIMŠEK, P.; DOVČ, A. Prevalence of antibodies against Leptospira sp. in snakes, lizards and turtles in Slovenia. Acta Veterinaria Scandinavica, Nova York, v. 55, n. 65, p. 1-8, 2013. DOI: 10.1186/1751-0147-55-65
LOPES, A. L. S.; SILVA, W. B.; PADOVANI, C. R.; LANGONI, H.; MODOLO, J. R. Frequência sorológica antileptospírica em cães: sua correlação com roedores e fatores ambientais, em área territorial urbana. Arquivos do Instituto Biológico, São Paulo, v. 72, n. 3, p. 289-296, 2005.
MACHADO, F. M. E.; COELHO, H. E.; REZENDE, R. S. Plano de ação para o 28 controle da leptospirose no Zoológico Municipal de Uberaba-MG. Bioscience Journal., Uberlândia, v. 26, n. 6, p. 981-989, 2010.
OLIVEIRA, J. P.; KAWANAMI, A. E.; SILVA, A. S. L.; CHUNG, D. G.; WERTHER, K. Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy’s side-necked turtle) in urban environment. Acta Tropica, Basel, v. 164, p. 165-168, 2016. DOI: 10.1016/j.actatropica.2016.08.019
RUEDA-ALMONACID, J. V.; CARR, J. L.; MITTERMEIER, R. A.; RODRÍGUEZ-MAHECHA, J. V.; MAST, R. B.; VOGT, R. C.; RHODIN, A. G. J.; OSSAVELÁSQUEZ, J. de LA; RUEDA, J. N.; MITTERMEIER, C. G. Las tortugas y los cocodrilianos de los países andinos del trópico. Serie de guías tropicales de campo Nº 6. Conservación Internacional. Editorial Panamericana, Formas e Impresos. Bogotá, 2007. 538 p.
SILVA, C. S.; GÍRIO, R. J. S.; GUERRA NETO, G.; BRICH, M.; SANTANA, L. A. S.; AMÂNCIO, F. H.; MARIANI, J. R.; WESSORT, P. M. F. Anticorpos anti-Leptospira spp. em animais selvagens do zoológico municipal de Ribeirão Preto, Estado de São Paulo, Brasil. Brazilian Journal of Veterinary Research and Animal Science, São Paulo, v. 47, n. 3, p. 237-242, 2010. DOI: 10.11606/issn.1678-4456.bjvras.2010.26862
SILVA, E. F.; SEYFFERT, N.; CERQUEIRA, G. M. Serum antileptospiral agglutinins in freshwater turtles from Southern Brazil. Brazilian Journal of Microbiology, São Paulo, v. 40, n. 2, p. 227-230, 2009. DOI: 10.1590/S1517-83822009000200003
ANDREWS, R. D.; REILLY, J. R.; FERRIS, D.; HANSON, L. E. Leptospiral agglutinins in sera from Southern Illinois Herpetofauna. Journal of Wildlife Diseases, University of Illinois at Urbana-Champaign, v. 1, n.4, p. 55-59, 1965. DOI: 10.7589/0090-3558-1.4.55
CARDOSO, S. L. C. Tomada de decisão em Jardim Botânico: Bosque Rodrigues Alves-Jardim Zoobotânico da Amazônia (BRAJZBA). Amazônia, Organizações e Sustentabilidade, Belém, v. 6, n. 2, p. 123-131, 2018. DOI: http://dx.doi.org/10.17800/2238-8893/aos.v6n2jul/dez2017p123-131
DEZZUTTO, D.; BARBERO, R.; CANALE, L.; ACUTIS, P. L.; BIOLATTI, C.; DOGLIERO, A.; MITZY, M. D.; FRANCONE, P.; COLZANI, A.; BERGAGNA, S.; GENNERO, M. S. Detection of Leptospira spp. in water turtle (Trachemys scripta) living in ponds of urban parks. Veterinary Sciences, Basel, v. 4, n. 4, p. 51-55, 2017. DOI: 10.3390/vetsci4040051
EBANI, V. V. Domestic reptiles as source of zoonotic bacteria: a mini review. Asian Pacific Journal of Tropical Medicine, Haikou, v. 10, n. 8, p. 723-728, 2017. DOI: 10.1016/j.apjtm.2017.07.020
ESTEVES, F. M.; GUERRA-NETO, G.; GIRIO, R. J. S.; SILVA-VERGARA, M. L.; CARVALHO, A. C. F. B. Detecção de anticorpos para Leptospira spp. em animais e funcionários do zoológico municipal de Uberaba, MG. Arquivos do Instituto Biológico, São Paulo, v. 72, n. 3, p. 283-288, 2005.
FIGUEIREDO, M. W. Estrutura populacional, uso de ambientes e crescimento corporal de Rhinoclemmys punctularia punctularia (Daudin, 1801), na Ilha de Algodoal/ Maiandeua, Maracanã, Pará, Brasil. 2010. Dissertação (Mestrado) - Instituto de Ciências Biológicas, Universidade Federal do Pará, Pará.
FRETEY, J.; BILLES, A.; TIWARI, M. Leatherback, Dermochelys coriacea, nesting along the Atlantic coast of Africa. Chelonian Conservation Biology, v. 6, n. 1, p. 126-129, 2007. DOI: 10.2744/1071-8443
GLOSSER, J. W.; SULZER, C. R.; EBERHARDT, M.; WINKLER, W. G. Cultural and serologic evidence of Leptospira interrogans serotype Tarassovi infection in turtles. Journal of Wildlife. Diseases, Massachusetts, v. 10, n. 4, p. 429-435, 1974. DOI: 10.7589/0090-3558-10.4.429
GRIMM, K.; MITCHELL, M. A.; THOMPSON, D.; MADDOX, C. Seroprevalence of Leptospira spp. in Blanding's Turtles (Emydoidea blandingii) from DuPage County, Illinois USA. Journal of Herpetological Medicine and Surgery, Gainesville, v. 25, n. 1-2, p. 28-32, 2015. DOI: 10.5818/1529-9651-25.1.28
KIKTENKO, V.S.; ANANYIN, V. Leptospiroz na daljnem vostoke (leptospirose do Extremo Oriente). Zeitschrift für allgemeine Mikrobiologie, Moscou, n. 12, p. 72-78, 1941.
LINDTNER-KNIFIC, R.; VERGLES-RATAJ, A.; VLAHOVIĆ, K.; ZRIMŠEK, P.; DOVČ, A. Prevalence of antibodies against Leptospira sp. in snakes, lizards and turtles in Slovenia. Acta Veterinaria Scandinavica, Nova York, v. 55, n. 65, p. 1-8, 2013. DOI: 10.1186/1751-0147-55-65
LOPES, A. L. S.; SILVA, W. B.; PADOVANI, C. R.; LANGONI, H.; MODOLO, J. R. Frequência sorológica antileptospírica em cães: sua correlação com roedores e fatores ambientais, em área territorial urbana. Arquivos do Instituto Biológico, São Paulo, v. 72, n. 3, p. 289-296, 2005.
MACHADO, F. M. E.; COELHO, H. E.; REZENDE, R. S. Plano de ação para o 28 controle da leptospirose no Zoológico Municipal de Uberaba-MG. Bioscience Journal., Uberlândia, v. 26, n. 6, p. 981-989, 2010.
OLIVEIRA, J. P.; KAWANAMI, A. E.; SILVA, A. S. L.; CHUNG, D. G.; WERTHER, K. Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy’s side-necked turtle) in urban environment. Acta Tropica, Basel, v. 164, p. 165-168, 2016. DOI: 10.1016/j.actatropica.2016.08.019
RUEDA-ALMONACID, J. V.; CARR, J. L.; MITTERMEIER, R. A.; RODRÍGUEZ-MAHECHA, J. V.; MAST, R. B.; VOGT, R. C.; RHODIN, A. G. J.; OSSAVELÁSQUEZ, J. de LA; RUEDA, J. N.; MITTERMEIER, C. G. Las tortugas y los cocodrilianos de los países andinos del trópico. Serie de guías tropicales de campo Nº 6. Conservación Internacional. Editorial Panamericana, Formas e Impresos. Bogotá, 2007. 538 p.
SILVA, C. S.; GÍRIO, R. J. S.; GUERRA NETO, G.; BRICH, M.; SANTANA, L. A. S.; AMÂNCIO, F. H.; MARIANI, J. R.; WESSORT, P. M. F. Anticorpos anti-Leptospira spp. em animais selvagens do zoológico municipal de Ribeirão Preto, Estado de São Paulo, Brasil. Brazilian Journal of Veterinary Research and Animal Science, São Paulo, v. 47, n. 3, p. 237-242, 2010. DOI: 10.11606/issn.1678-4456.bjvras.2010.26862
SILVA, E. F.; SEYFFERT, N.; CERQUEIRA, G. M. Serum antileptospiral agglutinins in freshwater turtles from Southern Brazil. Brazilian Journal of Microbiology, São Paulo, v. 40, n. 2, p. 227-230, 2009. DOI: 10.1590/S1517-83822009000200003
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Published
2019-10-16
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Rocha, K. de S., Baia, I. W. M., Monteiro, L. H., Miranda, J. M. S., Monteiro, T. R. M., Silva, A. F. da, … Moraes, C. C. G. de. (2019). Identification of antibodies to Leptospira spp. in the spot-legged turtle (Rhinoclemmys punctularia) maintained in captivity. Semina: Ciências Agrárias, 40(6Supl3), 3763–3768. https://doi.org/10.5433/1679-0359.2019v40n6Supl3p3763
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