Estimação do tamanho de parcela para experimento com cultura de tecidos em videira

Augusto Ramalho de Morais; Aparecida Gomes de Araújo; Moacir Pasqual; Ana Patricia Bastos Peixoto

doi:10.5433/1679-0359.2014v35n1p113

Estimation of plot size for experiments with tissue culture in grape

Authors

Augusto Ramalho de Morais Universidade Federal de Lavras
Aparecida Gomes de Araújo Syngenta Seeds Ltda
Moacir Pasqual Universidade Federal de Lavras
Ana Patricia Bastos Peixoto Universidade Estadual da Paraíba

DOI:

https://doi.org/10.5433/1679-0359.2014v35n1p113

Keywords:

Vitis sp., Coefficient of variation, Sample size, Experimental precision, Uniformity trial, Planning experimental.

Abstract

The improvement of experimental techniques of in vitro culture may provide better identification and multiplication of most promising materials. The aim of this study was to estimate the plot size (explants numbers) for in vitro micropropagation grape. 15 tests were conducted uniformity formed by combinations of three rootstock vine with five different culture media. Nodes segments were used with about 2.5 cm, were excised and inoculated. After 90 days of inoculation, the variables shoot number, shoot length, shoot fresh weight, and fresh weight of callus were evaluated. For each assay, was simulated plots of differents sizes , with each tube containing one explant was considered as a basic unit. For estimation the optimum plot size we used the modified maximum curvature method and the least significant difference between means was obtained by the method of Hatheway. The results showed that the estimates of the plot size ranged from five (5) to 12 explants (tubes) in accordance with the variable used. For these variables, the optimal plot size should be formed by 12 explants.

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Author Biographies

Augusto Ramalho de Morais, Universidade Federal de Lavras

Engº Agrº, Prof. Associado do Deptº de Ciências Exatas da Universidade Federal de Lavras, UFLA, Lavras, MG. Bolsista de produtividade do CNPq.

Aparecida Gomes de Araújo, Syngenta Seeds Ltda

Engª Agrª, Pesquisadora, Drª da Syngenta Seeds Ltda, Uberlândia, MG.

Moacir Pasqual, Universidade Federal de Lavras

Engº Agrº, Prof. Titular do Deptº de Agricultura, UFLA, Lavras, MG. Bolsista de produtividade do CNPq.

Ana Patricia Bastos Peixoto, Universidade Estadual da Paraíba

Engª Agrª, Profª Adjunta do Deptº de Estatística, Universidade Estadual da Paraíba, UEPB, Campina Grande, PB.

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Published

2014-02-26

How to Cite

Morais, A. R. de, Araújo, A. G. de, Pasqual, M., & Peixoto, A. P. B. (2014). Estimation of plot size for experiments with tissue culture in grape. Semina: Ciências Agrárias, 35(1), 113–124. https://doi.org/10.5433/1679-0359.2014v35n1p113

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Issue

Vol. 35 No. 1 (2014)

Section

Articles

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