Light intensity, blood fraction, fasting and storage time affect blood biochemical metabolites in broiler chickens
DOI:
https://doi.org/10.5433/1679-0359.2023v44n6p2095Palabras clave:
Blood components, Lux, Plasma, Poultry, Serum.Resumen
We determined the impacts of light intensity, blood fraction, fasting and storage time on glucose, cholesterol, triglycerides, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and gamma-glutamyl transferase (GGT) concentrations in 140 broiler chickens assigned to two light intensities (5 or 20 lux/m²) and seven fasting times (0, 2, 4, 6, 8, 10, and 12 h). The collected blood was fractionated into serum or plasma and stored at -20°C for 0, 15, 30, 60, and 120 days. Serum glucose concentrations declined linearly by 1.15 mg dL-1 every 2 h of fasting, whereas plasma glucose responded quadratically (minimum point at 8 h and 13 min of fasting). Serum ALT concentrations showed a quadratic effect, with a maximum point at 3 h and 46 min of fasting. Serum GGT concentrations were not influenced by fasting time, whereas plasma decreased by 1.60 IU L-1 at each 2 h of fasting. The AST concentrations were influenced by fasting. Storage times influenced the glucose concentrations in a quadratic manner, with maximum points in serum and plasma at 95 and 72 days, respectively. Serum cholesterol and triglyceride concentrations increased linearly with storage time. Storage time caused a quadratic response on serum ALT, with a maximum point at 32 days, whereas there was a minimum point at 93 days in plasma. Concentrations of GGT increased linearly with storage. Glucose, triglycerides, AST, and ALT concentrations were highest at 20 lux. Samples for AST analysis can be stored up to 120 days at -20°C. Serum glucose, cholesterol, triglyceride, ALT, and GGT analysis is indicated with a 6 h fasting time, without freezing. The light intensity should be specified.
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Al-Kharusi, A., Al-Lawati, N., Al-Kindi, M., & Mula-Abed, W. A. (2014). Are tubes containing sodium fluoride still needed for the measurement of blood glucose in hospital laboratory practice? Oman Medical Journal, 29(6), 404-409. doi: 10.5001/omj.2014.109 DOI: https://doi.org/10.5001/omj.2014.109
Allain, C. C., Poon, L. S., Chan, C. S., Richmond, W. F. P. C., & Fu, P. C. (1974). Enzymatic determination of total serum cholesterol. Clinical Chemistry, 20(4), 470-475. doi: 10.1093/clinchem/20.4.470 DOI: https://doi.org/10.1093/clinchem/20.4.470
Alper, C. (1974). Specimen collection and preservation. In R. J. Henry, D. C. Cannon, & J. W. Winkelman (Eds.), Clinical chemistry, principles and techniques (pp. 373-388). New York.
Andrade, T. D. S., Nunes, R. V., Silva, I. M. da, Wachholz, L., & Fascina, V. B. (2017). Performance and physiological parameters in broilers fed different enzyme complexes. Semina: Ciências Agrárias, 38(4 Suppl. 1), 2765-2774. doi: 10.5433/1679-0359.2017v38n4Supl1p2765 DOI: https://doi.org/10.5433/1679-0359.2017v38n4SUPLp2765
Behboudi, H., Esmaeilipour, O., Mirmahmoudi, R., & Mazhari, M. (2016). The influence of drinking water containing lemon juice and thyme supplemented diet on performance and some blood parameters of broilers under heat stress. Iranian Journal of Applied Animal Science, 6(1), 169-174.
Bonetti, G., Cancelli, V., Coccoli, G., Piccinelli, G., Brugnoni, D., Caimi, L., & Carta, M. (2016). Which sample tube should be used for routine glucose determination? Primary Care Diabetes, 10(3), 227-232. doi: 10.1016/j.pcd.2015.11.003 DOI: https://doi.org/10.1016/j.pcd.2015.11.003
Burtis, C. A., Ashwood, E. R., & Bruns, D. E. (2012). Tietz textbook of clinical chemistry and molecular diagnostics-e-book (5nd ed.). Elsevier Saunders.
Clark, M. L., Humphreys, S. M., & Frayn, K. N. (1990). Stability of plasma glucose during storage. Annals of Clinical Biochemistry, 27(4), 373-377. doi: 10.1177/000456329002700415 DOI: https://doi.org/10.1177/000456329002700415
Córdova-Noboa, H. A., Oviedo-Rondón, E. O., Sarsour, A. H., Barnes, J., Sapcota, D., López, D., Gross, L., Rademacher-Heilshorn, M., & Braun, U. (2018). Effect of guanidinoacetic acid supplementation on live performance, meat quality, pectoral myopathies and blood parameters of male broilers fed corn-based diets with or without poultry by-products. Poultry Science, 97(7), 2494-2505. doi: 10.3382/ps/pey097 DOI: https://doi.org/10.3382/ps/pey097
Cray, C., Rodriguez, M., Zaias, J., & Altman, N. H. (2009). Effects of storage temperature and time on clinical biochemical parameters from rat serum. Journal of the American Association for Laboratory Animal Science, 48(2), 202-204.
Cuhadar, S., Koseoglu, M., Atay, A., & Dirican, A. (2013). The effect of storage time and freeze-thaw cycles on the stability of serum samples. Biochemia Medica, 23(1), 70-77. doi: 10.11613/BM.2013.009 DOI: https://doi.org/10.11613/BM.2013.009
Divya, P. D., & Jayavardhanan, K. K. (2010). Effect of temperature and storage time on hepatobiliary enzyme activities in goat serum. Veterinary World, 3(6), 277-279.
Du, P., Wang, H., Shi, X., Zhang, X., Zhu, Y., Chen, W., & Huang, Y. (2023). A comparative study to determine the effects of breed and feed restriction on glucose metabolism of chickens. Animal Nutrition, 13, 261-269. doi: 10.1016/j.aninu.2023.02.005 DOI: https://doi.org/10.1016/j.aninu.2023.02.005
Evans, K., Mitcheson, J., & Laker, M. F. (1995). Effect of storage at 4 degrees C and-20 degrees C on lipid, lipoprotein, and apolipoprotein concentrations. Clinical Chemistry, 41(3), 392-396. doi: 10.1093/clinchem/41.3.392 DOI: https://doi.org/10.1093/clinchem/41.3.392
Fernandez, L., Jee, P., Klein, M. J., Fischer, P., Perkins, S. L., & Brooks, S. P. (2013). A comparison of glucose concentration in paired specimens collected in serum separator and fluoride/potassium oxalate blood collection tubes under survey ‘field’conditions. Clinical Biochemistry, 46(4-5), 285-288. doi: 10.1016/j.clinbiochem.2012.11.027 DOI: https://doi.org/10.1016/j.clinbiochem.2012.11.027
Fidan, E. D., Nazlıgül, A., Türkyılmaz, M. K., Aypak, S. Ü., Kilimci, F. S., Karaarslan, S., & Kaya, M. (2017). Effect of photoperiod length and light intensity on some welfare criteria, carcass, and meat quality characteristics in broilers. Revista Brasileira de Zootecnia, 46(3), 202-210. doi: 10.1590/S1806-92902017000300004 DOI: https://doi.org/10.1590/s1806-92902017000300004
Fossati, P., & Prencipe, L. (1982). Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clinical Chemistry, 28(10), 2077-2080. doi: 10.1093/clinchem/28.10.2077 DOI: https://doi.org/10.1093/clinchem/28.10.2077
Gilani, S. M. H., Zehra, S., Galani, S., & Ashraf, A. (2018). Effect of natural growth promoters on immunity, and biochemical and haematological parameters of broiler chickens. Tropical Journal of Pharmaceutical Research, 17(4), 627-633. doi: 10.4314/tjpr.v17i4.9 DOI: https://doi.org/10.4314/tjpr.v17i4.9
Grande, F., Amatuzio, D. S., & Wada, S. (1964). Cholesterol measurement in serum and in plasma. Clinical Chemistry, 10(7), 619-626. doi: 10.1093/clinchem/10.7.619 DOI: https://doi.org/10.1093/clinchem/10.7.619
Hagan, J. K., Hagan, B. A., Ofori, S. A., & Etim, N. N. (2022). Haematological and serum biochemical profiles of two broiler strains fed rations with varying levels of palm kernel oil residue. Ghanaian Journal of Animal Science, 13(2), 30-41.
Hosseini-Vashan, S. J., Golian, A., & Yaghobfar, A. (2016). Growth, immune, antioxidant, and bone responses of heat stress-exposed broilers fed diets supplemented with tomato pomace. International Journal of Biometeorology, 60, 1183-1192. doi: 10.1007/s00484-015-1112-9 DOI: https://doi.org/10.1007/s00484-015-1112-9
Islam, S. K. M. A., Alauddin, M., Hassan, M. M., Khan, S. A., Alam, M. R., Hossain, M. B., Ahasan, A. S. M. L., Saifuddin, A. K. M., Sultana, S., Tun, H. M., Shaikat, A. H., Debnath, N. C., & Hoque, M. A. (2012). Biochemical analysis on blood and crop contents of household chickens along with their production and health status in Bangladesh. Pakistan Veterinary Journal, 32(4), 575-578.
Kaneko, J. J., Harvey, J. W., & Bruss, M. L. (2008). Clinical biochemistry of domestic animals (6nd ed.). Academic Press.
Kelly, L. M., & Alworth, L. C. (2013). Techniques for collecting blood from the domestic chicken. Lab Animal, 42(10), 359-361. doi: 10.1038/laban.394 DOI: https://doi.org/10.1038/laban.394
Kim, J. H., Choi, H. S., Goo, D., Park, G. H., Han, G. P., Reyes, J. D., & Kil, D. Y. (2019). Effect of dietary melamine concentrations on growth performance, excreta characteristics, plasma measurements, and melamine residue in the tissue of male and female broiler chickens. Poultry Science, 98(8), 3204-3211. doi: 10.3382/ps/pez050 DOI: https://doi.org/10.3382/ps/pez050
Lumeij, J. T. (2008). Avian clinical biochemistry. In J. J. Kaneko, J. W. Harvey, & M. L. Bruss (Eds.), Clinical biochemistry of domestic animals (pp. 839-872). San Diego. DOI: https://doi.org/10.1016/B978-0-12-370491-7.00030-1
Oke, E., Ladokun, A. O., Daramola, J. O., & Onagbesan, O. M. (2017). Haematology and serum biochemical profile of laying chickens reared on deep litter system with or without access to grass or legume pasture under humid tropical climate. International Journal of Animal and Veterinary Sciences, 11(2), 91-96. doi: 10.5281/zenodo.1339830
Olanrewaju, H. A., Purswell, J. L., Collier, S. D., & Branton, S. L. (2010). Effect of ambient temperature and light intensity on physiological reactions of heavy broiler chickens. Poultry Science, 89(12), 2668-2677. doi: 10.3382/ps.2010-00806 DOI: https://doi.org/10.3382/ps.2010-00806
Oliveira, F. S., Falbo, M. K., Sandini, I. E., & Ishiy, L. E. (2011). Efeito do congelamento e do tempo de armazenamento do soro sanguíneo de cordeiros na determinação de parâmetros bioquímicos. Semina: Ciências Agrárias, 32(2), 717-721. doi: 10.5433/1679-0359.2011v32n2p717 DOI: https://doi.org/10.5433/1679-0359.2011v32n2p717
Pini, C., Sommariva, D., Branchi, A., Scandiani, L., & Fasoli, A. (1990). Effects of serum storage on the determination of cholesterol. Research in Clinic and Laboratory, 20, 37-44. doi: 10.1007/BF02910147 DOI: https://doi.org/10.1007/BF02910147
Puvadolpirod, S., & Thaxton, J. P. (2000). Model of physiological stress in chickens 1. Response parameters. Poultry Science, 79(3), 363-369. doi: 10.1093/ps/79.3.363 DOI: https://doi.org/10.1093/ps/79.3.363
Rodrigues, D. R., Café, M. B., Jardim, R. M., Oliveira, E., Trentin, T. C., Martins, D. B., & Minafra, C. S. (2017). Metabolism of broilers subjected to different lairage times at the abattoir and its relationship with broiler meat quality. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 69(3), 733-741. doi: 10.1590/1678-4162-9268 DOI: https://doi.org/10.1590/1678-4162-9268
Rostagno, H. S., Albino, L. F. T., Hannas, M. I., Donzele, J. L., Sakomura, N. K., Perazzo, F. G., Saraiva, A., Teixeira, M. L., Rodrigues, P. B., Oliveira, R. F. D., Barreto, S. L. D. T., & Brito, A. O. (2017). Tabelas brasileiras para aves e suínos: composição de alimentos e exigências nutricionais (4a ed.). UFV.
Sabater, M., & Forbes, N. (2015). Avian haematology and biochemistry 2. Biochemistry. In Practice, 37(3), 139-142. doi: 10.1136/inp.g6976 DOI: https://doi.org/10.1136/inp.g6976
Sadeghi, A. A., Mohamadi-Saei, M., & Ahmadvand, H. (2014). The efficacy of dietary savory essential oil on reducing the toxicity of Aflatoxin B1 in broiler chicks. Kafkas Universitesi Veteriner Fakultesi Dergisi, 20(4), 481-486. doi: 10.9775/kvfd.2013.10217 DOI: https://doi.org/10.9775/kvfd.2013.10217
Schumann, G., Bonora, R., Ceriotti, R. F., Férard, G., Ferrero, C. A., Franck, P. F., Gella, F. J., Hoelze, W., Jorgensen, P. J., Kanno, T., Kessner, A., Klauke, R., Kristiansen, N., Lessinger, J. M., Linsinger, T. P. J., Misaki, H., Panteghini, M., Pauwels, J., Schiele, F., ... Siekmann, L. (2002a). IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37°C. Part 4. Reference procedure for the measurement of catalytic activity concentration of alanine aminotransferase. Journal of Clinical Chemistry and Laboratory Medicine, 40, 718-724. doi: 10.1515/CCLM.2002.124 DOI: https://doi.org/10.1515/CCLM.2002.124
Schumann, G., Bonora, R., Ceriotti, R. F., Férard, G., Ferrero, C. A., Franck, P. F., Gella, F. J., Hoelze, W., Jorgensen, P. J., Kanno, T., Kessner, A., Klauke, R., Kristiansen, N., Lessinger, J. M., Linsinger, T. P. J., Misaki, H., Panteghini, M., Pauwels, J., Schiele, F., ... Siekmann, L. (2002b). IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37°C. Part 5. Reference procedure for the measurement of catalytic activity concentration of aspartate-aminotransferase. Journal of Clinical Chemistry and Laboratory Medicine, 40, 725-733. doi: 10.1515/CCLM.2002.125 DOI: https://doi.org/10.1515/CCLM.2002.125
Schumann, G., Bonora, R., Ceriotti, R. F., Férard, G., Ferrero, C. A., Franck, P. F., Gella, F. J., Hoelze, W., Jorgensen, P. J., Kanno, T., Kessner, A., Klauke, R., Kristiansen, N., Lessinger, J. M., Linsinger, T. P. J., Misaki, H., Panteghini, M., Pauwels, J., Schiele, F., ... Siekmann, L.(2002c). IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37°C. Part 6. Reference procedure for the measurement of catalytic activity concentration of γ-glutamyltransferase. Journal of Clinical Chemistry and Laboratory Medicine, 40, 734-738. doi: 10.1515/CCLM.2002.126 DOI: https://doi.org/10.1515/CCLM.2002.126
Silva, B. R., Mareze, M., Falbo, M. K., Sandini, I. E., & Groff, P. M. (2015). Bioquimico plasmático de cães: efeitos dos diferentes anticoagulantes em comparação com o soro. Colloquium Agrariae, 11(1), 33-41. doi: 10.5747/ca.2015.v11.n1.a116 DOI: https://doi.org/10.5747/ca.2015.v11.n1.a116
Silva, É. P. D., Torres, M. D. M., Cruz, T. P. P. S. D., & Mendonça, A. J. (2017). Influência da temperatura e do tempo de armazenamento sobre amostras de soro e plasma caninos na análise da enzima alanina aminotransferase (ALT). Ciência Animal Brasileira, 18, 1-5. doi: 10.1590/1089-6891v18e-33785 DOI: https://doi.org/10.1590/1089-6891v18e-33785
Silva, I. M., Broch, J., Wachholz, L., Souza, C. de, Dalolio, F. S., Teixeira, L. V., Eyng, C., & Nunes, R. V. (2019). Dry residue of cassava associated with carbohydrases in diets for broiler chickens. Journal of Applied Poultry Research, 28(4), 1189-1201. doi: 10.3382/japr/pfz085 DOI: https://doi.org/10.3382/japr/pfz085
Spinelli, M. O., Cruz, R. J. D., Godoy, C. M. D. S. C., & Motta, M. C. D. (2012). Efeito da temperatura e tempo no armazenamento de metabólitos no plasma de ratos Wistar recém desmamados. Revista da Sociedade Brasileira de Ciência em Animais de Laboratório, 1(4), 317-321.
Subhani, Z., Shahid, M., Hussain, F., & Khan, J. A. (2018). Efficacy of Chlorella pyrenoidosa to ameliorate the hepatotoxic effects of aflatoxin B1 in broiler chickens. Pakistan Veterinary Journal, 38(1), 13-18. doi: 10.29261/pakvetj/2018.003 DOI: https://doi.org/10.29261/pakvetj/2018.003
Swarna, C. L., Rao, S. V., Srinivas, G., & Reddy, V. R. (2018). Effect of unsaturated to saturated fatty acids ratio of supplemental fat in the diet with or without l-carnitine on performance of broiler chicken. Indian Journal of Animal Nutrition, 35(1), 90-96. doi: 10.5958/2231-6744.2018.00012.9 DOI: https://doi.org/10.5958/2231-6744.2018.00012.9
Thoresen, S. I., Tverdal, A., Havre, G., & Morberg, H. (1995). Effects of storage time and freezing temperature on clinical chemical parameters from canine serum and heparinized plasma. Veterinary Clinical Pathology, 24(4), 129-133. doi: 10.1111/j.1939-165X.1995.tb00954.x DOI: https://doi.org/10.1111/j.1939-165X.1995.tb00954.x
Trinder, P. (1969). Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Annals of Clinical Biochemistry, 6(1), 24-27. doi: 10.1136/jcp.22.2.158 DOI: https://doi.org/10.1177/000456326900600108
Veiga, J. A., Roselino, E. S., & Migliorini, R. H. (1978). Fasting, adrenalectomy, and gluconeogenesis in the chicken and a carnivorous bird. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 234(3), 115-121. doi: 10.1152/ajpregu.1978.234.3.R115 DOI: https://doi.org/10.1152/ajpregu.1978.234.3.R115
Wang, Y., Guo, Y., Ning, D., Peng, Y., Cai, H., Tan, J., Yang, Y., & Liu, D. (2012). Changes of hepatic biochemical parameters and proteomics in broilers with cold-induced ascites. Journal of Animal Science and Biotechnology, 3(41), 1-9. doi: 10.1186/2049-1891-3-41 DOI: https://doi.org/10.1186/2049-1891-3-41
Yang, J. Y., Zhang, H. J., Wang, J., Wu, S. G., Yue, H. Y., Jiang, X. R., & Qi, G. H. (2017). Effects of dietary grape proanthocyanidins on the growth performance, jejunum morphology and plasma biochemical indices of broiler chicks. Animal, 11(5), 762-770. doi: 10.1017/S1751731116002056 DOI: https://doi.org/10.1017/S1751731116002056
Yu, X., & Long, Y. C. (2016). Crosstalk between cystine and glutathione is critical for the regulation of amino acid signaling pathways and ferroptosis. Scientific Reports, 6(1), 1-10. doi: 10.1038/srep30033 DOI: https://doi.org/10.1038/srep30033
Zakaria, H. A., Jalal, M., Al-Titi, H. H., & Souad, A. (2017). Effect of sources and levels of dietary zinc on the performance, carcass traits and blood parameters of broilers. Brazilian Journal of Poultry Science, 19(03), 519-526. doi: 10.1590/1806-9061-2016-0415 DOI: https://doi.org/10.1590/1806-9061-2016-0415
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