Intake, digestibility and blood metabolites of lambs fed increasing levels of exogenous fibrolytic enzymes

Authors

DOI:

https://doi.org/10.5433/1679-0359.2024v45n4p1013

Keywords:

Sheep, Fibrolytic enzyme, Consumption.

Abstract

The present study aimed to evaluate how the increasing addition of exogenous fibrolytic enzymes in the diet of ewe lambs influenced feed intake, digestive capacity and blood metabolites. The trial was conducted in a 5×5 Latin square design with 5 treatments and 5 replications, using crossbred Santa Inês × Dorper ewe lambs with an average initial weight of 46.48 ± 5.60 kg and approximately 7 months of age. Over a period of 60 days, the animals were housed in individual metabolic cages. The treatments consisted of a control diet and four increasing levels of inclusion of fibrolytic enzymes (FIBROZYME®) (0.5, 1.0, 1.5, and 2.0% of dry matter), administered daily at the time of diet offering. There were no significant differences in nutrient intake (g animal−1 day−1), live weight (%PV−1), and metabolic weight (PV0.75) among treatments. There were also no significant differences in the apparent dry matter digestibility, fecal weight or fecal score. However, there was a significant difference in the blood metabolite creatinine but not in the animals’ blood glucose levels. Therefore, the addition of exogenous fibrolytic enzymes does not influence the feed intake or blood glucose of ewe lambs but alters plasma creatinine.

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Author Biographies

Ana Beatriz Inácio de Freitas, Universidade Federal de Uberlândia

Graduate Student of the Master's Program in Veterinary Sciences, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.

Lucas Eduardo Vilaça , Universidade Federal de Uberlândia

Graduate Student of the Master's Program in Veterinary Sciences, UFU Uberlândia, MG, Brazil.

Ariadne Freitas Silva , Universidade Federal de Uberlândia

PhD Student in the Veterinary Sciences Graduate Program, UFU, Uberlândia, MG, Brazil.

Luciano Fernandes de Sousa , Universidade Federal do Norte do Tocantins

Teacher. Doctor of the Undergraduate Course in Animal Science, Universidade Federal do Norte do Tocantins, UFNT, Araguaína, TO, Brazil.

Erica Beatriz Schultz , Universidade Federal de Viçosa

Teacher. Doctor of the Undergraduate Course in Animal Science, Universidade Federal de Viçosa, UFV, Viçosa, MG, Brazil.

Jhone Tallison Lira de Sousa, Universidade Federal de Uberlândia

PhD Student in the Veterinary Sciences Graduate Program, UFNT, Araguaína, TO, Brazil.

Karla Alves Oliveira, Universidade Estadual Paulista Júlio de Mesquita Filho

PhD Student in the Veterinary Sciences Graduate Program, Universidade Estadual Paulista Júlio de Mesquita Filho, UNESP, São Paulo, SP, Brazil.

Gilberto de Lima Macedo Júnior , Universidade Federal de Uberlândia

Teacher, Doctor of the Undergraduate Course in Animal Science, UFU, Uberlândia, MG, Brazil.

References

Adéola, O., & Cowieson, A. J., (2011). Opportunities and challenges in using exogenous to improve nonruminant animal production. National Center for Biotechnology Information, 89(10):3189-218. doi: 10.2527/jas.2010-3715. DOI: https://doi.org/10.2527/jas.2010-3715

Adesogan, A. T., Ma, Z. X., Romero, J. J., & Arriola, K. G. (2014). Ruminant nutrition symposium: improving cell wall digestion and animal performance with fibrolytic enzymes. Journal of Animal Science, 92(4), 1317-1330. doi: 10.2527/jas.2013-7273 DOI: https://doi.org/10.2527/jas.2013-7273

Al-Mamun, M., & Uddin, M. M. (2017). Effect of exogenous fibrolytic enzymes on growth performance and digestibility in Black Bengal goat. Journal of Animal Science Advances, (1), 2426-2433. doi: 10.32649/ajas.2021.176226 DOI: https://doi.org/10.32649/ajas.2021.176226

Araújo, C. M., Oliveira, K. A., Macedo, G. de. L. Jr., Silva, S. P. da & Silva, D. A. de P. (2020). Parâmetros nutricionais e bioquímicos de ovinos consumindo volumoso extrusado com diferentes teores de Uruchloa brizantha em comparação a silagem de milho tradicional. Caderno de Ciências Agrárias, 12(2020), 1-11. doi: 10.35699/2447-6218.2020.25810 DOI: https://doi.org/10.35699/2447-6218.2020.25810

Bartlett, M. S. (1937). Properties of sufficiency and statistical tests. Proceedings of the Royal Society, 160(901), 268-282. DOI: https://doi.org/10.1098/rspa.1937.0109

Beauchemin, K. A., Colombatto, D., Morgavi, D. P., & Yang, W. Z. (2003). Use of exogenous fibrolytic enzymes to improve feed utilization by ruminants. Journal of Animal Science, 81(2), 37-47. doi: 10.2527/2003.8114_suppl_2E37x

Berchielli, T. T., Pires, A. V., & Oliveira, S. G. (2011). Nutrição de ruminantes. Funep.

Brito, F. O. (2010). Enzimatic complex levels in ruminant diets. Dissertação de mestrado, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brasil.

Cunniff, P. (1995). Association of official analytical chemists (16nd ed.). Official Methods of Analysis.

Gomes, S. P., Borges, I., Borges, A. L. C. C., Macedo, G. de L., Jr., Campos, W. E., & Brito, T. S. (2012). Tamanho de partícula do volumoso e frequência de alimentação sobre o metabolismo energético e proteico em ovinos, considerando dietas com elevada participação de concentrado. Revista Brasileira de Saúde e Produção Animal, 13(3), 732-744. doi: 10.1590/S1519-99402012000300013 DOI: https://doi.org/10.1590/S1519-99402012000300013

Kozloski, G. V. (2011). Bioquímica dos ruminantes (3a rev. e amp.). Editora da UFSM.

Lynch, J. P., Jin, L., Church, J. S., Baah, J., & Beachemin, K. A. (2013). Fibrolytic enzymes and a ferulic acid esterase-producing bacterial additive applied to alfalfa hay at baling: effects on fibre digestibility, chemical composition and conservation characteristics. Grass and Forage Science, 70(1), 85-93. doi: 10.1111/gfs.12093 DOI: https://doi.org/10.1111/gfs.12093

Maynard, L. A., Loosli, J. K., Hintz, H. F., & Warner, R. G. (1984). Nutrição animal (3a ed.). F. Bastos.

Meyer, D. J., & Harvey, J. W. (2004). Veterinary laboratory medicine: interpretation & diagnosis. Saunders.

National Research Council (2007). Nutrient requirements of small ruminants. NRC.

Neiva, M. C., Schultz, E. B., Sousa, L. M., Oliveira, K. A., Sousa, L. F., & Macedo, G. de L., Jr. (2020). Exogenous enzymes in sheep diet: nutritional and physiological parameters. Acta Scientiarum. Animal Sciences, 44(1), 1-9. doi: 10.4025/actascianimsci.v44i1.56504 DOI: https://doi.org/10.4025/actascianimsci.v44i1.56504

Nelson, D. L., & Cox, M. M. (2014). Princípios de bioquímica de lehninger (6a ed.). Artmed.

Rodrigues, G. R. D., Siqueira, M. T. S., Dutra, T. O., Schutz, E. B., Sousa, L. F., & Macedo Júnior, G. de L, (2022). Uso de enzima amilolítica associada a enzimas proteolíticas e fibrolíticas na dieta para ovinos. Revista Agrária Acadêmica, 5(3), 2-14. doi: 10.32406/v5n3/2022/59-72/agrariacad DOI: https://doi.org/10.32406/v5n3/2022/59-72/agrariacad

Rojo, R., Mendoza, G. D., & Plata, F. X. (2007). Comparison of method of application on the effect of amylolytic enzymes on in vitro ruminal starch digestion. Journal of Applied Animal Research, 32(1), 81-84. doi: 10.1080/09712119.2007.9706852 DOI: https://doi.org/10.1080/09712119.2007.9706852

Shapiro, S. S., & Wilk, M. B. (1965). An analysis of variance test for normality. Biometrika, 52(3), 591-609. doi: 10.2307/2333709 DOI: https://doi.org/10.2307/2333709

Silva, D. A. de P., Santana, A. G., Araújo, C. M., Oliveira, K. A., Siqueira, M. T. S., & Macedo, G. de L., Jr. (2019). Avaliação dos efeitos nutricionais e metabólicos da substituição de silagem de milho por ração extrusada de Capim-marandu (Urochloa brizantha) em ovinos. Caderno de Ciências Agrárias, 12(2020), 1-9. doi: 10.35699/2447-6218.2020.19833 DOI: https://doi.org/10.35699/2447-6218.2020.19833

Silva, J. F. C., & Leão, M. I. (1979). Fundamentos de nutrição de ruminantes. Livroceres.

Sniffen, C. J., O’connor, D. J., Van Soest, P. J., Fox, D. G., & Russell, J. B. (1992). A net carbohydrate and protein system for evaluating cattle diets: carbohydrate and protein availability. Journal of Animal Science, 70(11), 3562-3577. doi: 10.2527/1992.70113562x DOI: https://doi.org/10.2527/1992.70113562x

Subramaniyan, S., & Prema, P. (2002). Biotechnology of microbial xylanases: enzymology, molecular biology, and application. Critical Reviews in Biotechnology, 22(1), 33-64. doi: 10.1080/07388550290789450 DOI: https://doi.org/10.1080/07388550290789450

Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991). Methods of dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Animal Science, 74(10), 3583-3597. doi: 10.3168/jds.s0022-0302(91)78551-2 DOI: https://doi.org/10.3168/jds.S0022-0302(91)78551-2

Varanis, L. F. M., Schutz, E. B., Oliveira, K. A., Sousa, L. F., Cruz, W. F. G., & Macedo, G. de L., Jr. (2020). Intervalos de referência de bioquímicos séricos para cordeiros do nascimento a um ano nos trópicos. Semina: Ciências Agrárias, 42(3), 1725-1740. doi: 10.5433/1679-0359.2021v42n3Supl1p1725 DOI: https://doi.org/10.5433/1679-0359.2021v42n3Supl1p1725

Weiss, W. P. (1999). Energy prediction equations for ruminant feeds. Cornell University.

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Published

2024-06-25

How to Cite

Freitas, A. B. I. de, Vilaça , L. E., Silva , A. F., Sousa , L. F. de, Schultz , E. B., Sousa, J. T. L. de, … Macedo Júnior , G. de L. (2024). Intake, digestibility and blood metabolites of lambs fed increasing levels of exogenous fibrolytic enzymes . Semina: Ciências Agrárias, 45(4), 1013–1030. https://doi.org/10.5433/1679-0359.2024v45n4p1013

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