Control agents on the quality of Africanized honeybee queens

Authors

DOI:

https://doi.org/10.5433/1679-0359.2020v41n5p1773

Keywords:

Apis mellifera. Selectivity. Non-target organism, Entomopathogenic fungus, Plant extract.

Abstract

The objective of this study was to evaluate the effect of the entomopathogenic fungus Beauveria bassiana, used in large field crops, and of the pomegranate Punica granatum aqueous extract, with insecticidal potential for insect control, on the quality of Africanized Apis mellifera queens. The control sample was composed of sterile distilled water with Tween®. The treatments were incorporated into a voile fabric, covered with an acrylic plate and packed with queen rearing nucleus hive, so the worker bees could be in contact with control agents (B. bassiana and P. granatum). In the next day, frames with 30 cell cups for queen rearing with larvae were introduced to produce queens, which were monitored at moment of emergency. Measurements included live weight, wing size, abdomen size, thorax size, the time of emergence, and analysis of the breeding area size. Histological analyses of the hypopharyngeal glands of attendant worker bees and of the midgut of virgin queens were performed. There was no reduction in the weight of queens, no changes in the length, width, and height of their thoraxes, no changes in the width and length of the wing, and no changes in total length. However, B. bassiana increased the weight of the queens and reduced emergency time. The control agents did not interfere in villi height and breeding area. No differences were found in tissues when the treatments were compared with their control. P. granatum extract and the fungus B. bassiana did not interfere in the quality of Africanized A. mellifera queens.

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Author Biographies

Michele Potrich, Universidade Tecnológica Federal do Paraná

Profa Dra, Coordenação de Ciências Biológicas, Laboratório de Controle Biológico, Universidade Tecnológica Federal do Paraná, UTFPR, Campus Dois Vizinhos, Dois Vizinhos, PR, Brasil.

Fernanda Caroline Colombo, Universidade Tecnológica Federal do Paraná

Discente do Curso de Mestrado do Programa de Pós-Graduação em Agroecossistemas, PPGSIS, UTFPR, Dois Vizinhos, PR, Brasil.

Adriane Cristina Zanon, Universidade Tecnológica Federal do Paraná

Discente do Curso de Mestrado, Programa de Pós-Graduação em Agronomia, PPGAG, UTFPR, Pato Branco, PR, Brasil.

Everton Ricardi Lozano, Universidade Tecnológica Federal do Paraná

Prof. Dr., Coordenação de Ciências Biológicas, Laboratório de Controle Biológico, Universidade Tecnológica Federal do Paraná, UTFPR, Campus Dois Vizinhos, Dois Vizinhos, PR, Brasil.

Rodrigo Mendes Antunes Maciel, Universidade Tecnológica Federal do Paraná

Discente do Curso de Mestrado, Programa de Pós-Graduação em Zootecnia, PPGZO, UTFPR, Dois Vizinhos, PR, Brasil.

Flavia Galvan Tedesco, Universidade Tecnológica Federal do Paraná

Discente do Curso de Mestrado do Programa de Pós-Graduação em Agroecossistemas, PPGSIS, UTFPR, Dois Vizinhos, PR, Brasil.

Marisa Clemente Rodrigues, Universidade Tecnológica Federal do Paraná

Discente do Curso de Mestrado, Programa de Pós-Graduação em Zootecnia, PPGZO, UTFPR, Dois Vizinhos, PR, Brasil.

Fabiana Martins Costa-Maia, Universidade Tecnológica Federal do Paraná

Profª Drª, Coordenação de Zootecnia, Unidade de Ensino e Pesquisa em Apicultura, UTFPR, Dois Vizinhos, PR, Brasil.

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Published

2020-06-17

How to Cite

Potrich, M., Colombo, F. C., Zanon, A. C., Lozano, E. R., Maciel, R. M. A., Tedesco, F. G., … Costa-Maia, F. M. (2020). Control agents on the quality of Africanized honeybee queens. Semina: Ciências Agrárias, 41(5), 1773–1762. https://doi.org/10.5433/1679-0359.2020v41n5p1773

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