Abstract
Corn cultivation continually demands new technologies, since average yields can still be increased. This study aimed to evaluate the response of high-yield corn to humic acids, isolated from organic manure compost, with or without the application of lime and fertilizer. The experiment was conducted at Milhão® (http://www.omilhao.com.br) in Florestal, Minas Gerais State, Brazil. Three treatments were used: (a) control, without liming, fertilization, or humic acids; (b) conventional farm cultivation, with liming and fertilization; (c) liming, fertilization, and humic acids applied to seeds. The experiment design was a randomized block with five replicates (fifteen experimental units). Lime was applied at 1.6 Mg ha-1, incorporated into total area. Fertilization treatments included 300 kg ha-1 NPK 08-28-16 at sowing, and 500 kg ha-1 NPK 20-00-20 in coverage. The concentration of humic acids used in both seed treatment was 10 mmol L-1 of C. Biometrics and biomass productivity were evaluated at harvest time. Humic acids, whether applied as a seed coating, increased yield by ~15% higher than conventional farm cultivation, and this difference was statistically significant. Therefore, the use of humic acids based plant regulators is positive and complementary to the inputs generally used for corn yield.References
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BALDOTTO, M. A.; ROCHA, J. E.; ANDRADE, F. D. P.; DEL GIÚDICE, M. P.; BALDOTTO, L. E. B. Plant stimulant humic acid extracted of organic waste recycled by composting combined with liming and fertilization. Semina: Ciências Agrárias, Londrina, v. 37, n. 6, p. 3955-3964, 2016. DOI: 10.5433/1679-0359
CANELLAS, L. P.; OLIVARES, F. L. Physiological responses to humic substances as plant growth promoter. Chemical and Biological Technologies in Agriculture, Londres, v. 1, n. 1, p. 13-14, 2014. DOI: 10.1186/2196-5641-1-3.
CANELLAS, L. P.; OLIVARES, F. L.; OKOROKOVA-FAÇANHA, A. L.; FAÇANHA, A. R. Humic acids isolated from earthworm compost enhances root elongation, lateral root emergence, and plasma membrane H-ATPase activity in roots. Plant Physiology, San Diego, v. 130, n. 4, p. 1951-1957, 2002. DOI: 10.1104/pp.007088.
EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA - EMBRAPA. Tratamento e manejo de dejetos de bovinos. Juiz de Fora: EMBRAPA, 2001. 2 p. (Instrução técnica, 52).
GALVÃO, J. C. C.; BORÉM, A.; PIMENTEL, M. A. (Ed.). Milho: do plantio à colheita. Viçosa, MG: Editora UFV, 2015. 382 p.
INTERNATIONAL HUMIC SUBSTANCES SOCIETY - IHSS. Natural organic matter research. Isolation of IHSS samples. Madison: Soil Science Society of America, 2017. Available at: www.humicsubstances. Accessed at: 15 nov. 2018.
LOPES, S. J.; LÚCIO, A. D. C.; STORCK, L.; DAMO, H. P.; BRUM, B.; SANTOS, V. J. Relações de causa e efeito em espigas de milho relacionadas aos tipos de híbridos. Ciência Rural, Santa Maria, v. 37, n. 6, p. 1536-1542, 2007. DOI: 10.1590/S0103-84782007000600005.
MACMILLAN, T.; BENTON, T. G. Engage farmers in research: a new wave of small-scale agricultural innovation will boost yields and protect the planet. Nature, Londres, v. 509, n. 1, p. 25-27, 2014. DOI: 10.1038/509025a.
MELO, R. O.; BALDOTTO, L. E. B.; BALDOTTO, M. A. Corn initial vigor in response to humic acids from bovine manure and poultry litter. Semina: Ciências Agrárias, Londrina, v. 36, n. 3, p. 1863-1874, 2015. DOI: 10.5433/1679-0359.
NARDI, S.; PIZZEGHELLO, D.; MUSCOLO, A.; VIANELLO, A. Physiological effects of humic substances on higher plants. Soil Biology and Biochemistry, Amesterdã, v. 34, n. 11, p. 1527-1536, 2002. DOI: 10.1016/S0038-0717(02)00174-8.
PICCOLO, A. The supramolecular structure of humic substances. Soil Science, New York, v. 166, n. 1, p. 810-832, 2001. DOI: 10.1016/S0065-2113(02)75003-7.
TILMAN, D.; BALZER, C.; HILL, J.; BEFORT, B. L. Global food demand and the sustainable intensification of agriculture. Proceedings of the National Academy of Sciences, Washington, v. 108, n. 50, p. 20260-20264, 2011. DOI: 10.1073/pnas.1116437108.
BALDOTTO, M. A.; BALDOTTO, L. E. B. Ácidos húmicos. Revista Ceres, Viçosa, MG, v. 61, p. 856-881, 2014. Suplemento. DOI: 10.1590/s0034-737x201461000011.
BALDOTTO, M. A.; ROCHA, J. E.; ANDRADE, F. D. P.; DEL GIÚDICE, M. P.; BALDOTTO, L. E. B. Plant stimulant humic acid extracted of organic waste recycled by composting combined with liming and fertilization. Semina: Ciências Agrárias, Londrina, v. 37, n. 6, p. 3955-3964, 2016. DOI: 10.5433/1679-0359
CANELLAS, L. P.; OLIVARES, F. L. Physiological responses to humic substances as plant growth promoter. Chemical and Biological Technologies in Agriculture, Londres, v. 1, n. 1, p. 13-14, 2014. DOI: 10.1186/2196-5641-1-3.
CANELLAS, L. P.; OLIVARES, F. L.; OKOROKOVA-FAÇANHA, A. L.; FAÇANHA, A. R. Humic acids isolated from earthworm compost enhances root elongation, lateral root emergence, and plasma membrane H-ATPase activity in roots. Plant Physiology, San Diego, v. 130, n. 4, p. 1951-1957, 2002. DOI: 10.1104/pp.007088.
EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA - EMBRAPA. Tratamento e manejo de dejetos de bovinos. Juiz de Fora: EMBRAPA, 2001. 2 p. (Instrução técnica, 52).
GALVÃO, J. C. C.; BORÉM, A.; PIMENTEL, M. A. (Ed.). Milho: do plantio à colheita. Viçosa, MG: Editora UFV, 2015. 382 p.
INTERNATIONAL HUMIC SUBSTANCES SOCIETY - IHSS. Natural organic matter research. Isolation of IHSS samples. Madison: Soil Science Society of America, 2017. Available at: www.humicsubstances. Accessed at: 15 nov. 2018.
LOPES, S. J.; LÚCIO, A. D. C.; STORCK, L.; DAMO, H. P.; BRUM, B.; SANTOS, V. J. Relações de causa e efeito em espigas de milho relacionadas aos tipos de híbridos. Ciência Rural, Santa Maria, v. 37, n. 6, p. 1536-1542, 2007. DOI: 10.1590/S0103-84782007000600005.
MACMILLAN, T.; BENTON, T. G. Engage farmers in research: a new wave of small-scale agricultural innovation will boost yields and protect the planet. Nature, Londres, v. 509, n. 1, p. 25-27, 2014. DOI: 10.1038/509025a.
MELO, R. O.; BALDOTTO, L. E. B.; BALDOTTO, M. A. Corn initial vigor in response to humic acids from bovine manure and poultry litter. Semina: Ciências Agrárias, Londrina, v. 36, n. 3, p. 1863-1874, 2015. DOI: 10.5433/1679-0359.
NARDI, S.; PIZZEGHELLO, D.; MUSCOLO, A.; VIANELLO, A. Physiological effects of humic substances on higher plants. Soil Biology and Biochemistry, Amesterdã, v. 34, n. 11, p. 1527-1536, 2002. DOI: 10.1016/S0038-0717(02)00174-8.
PICCOLO, A. The supramolecular structure of humic substances. Soil Science, New York, v. 166, n. 1, p. 810-832, 2001. DOI: 10.1016/S0065-2113(02)75003-7.
TILMAN, D.; BALZER, C.; HILL, J.; BEFORT, B. L. Global food demand and the sustainable intensification of agriculture. Proceedings of the National Academy of Sciences, Washington, v. 108, n. 50, p. 20260-20264, 2011. DOI: 10.1073/pnas.1116437108.

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