Genetic variability of the fungus Beauveria spp. isolates and virulence to the lesser mealworm Alphitobius diaperinus Panzer (Coleoptera: Tenebrionidae)

Authors

  • Daian Guilherme Pinto de Oliveira Universidade Federal da Fronteira Sul - UFFS
  • Fabiana Gisele da Silva Pinto Universidade Estadual do Oeste do Paraná
  • Fernando Gomes Barcellos Faculdade Arthur Sá Earp Neto
  • Luis Francisco Angeli Alves Universidade Estadual do Oeste do Paraná
  • Mariangela Hungria Embrapa Soja

DOI:

https://doi.org/10.5433/1679-0359.2011v32n1p147

Keywords:

DNA polymorphism, Microbial control, RAPD, Entomopathogenic fungi.

Abstract

The genetic variability of 13 Beauveria spp. isolates was assessed by RAPD technique and verified the relationship between the molecular profiles generated and geographical location of isolates, the original host and pathogenicity to the lesser-mealworm Alphitobius diaperinus. Pathogenicity bioassays were carried with larvae and adult insects at fungal concentration of 109 conidia / mL, during a ten-day period. With RAPD technique, seven of 50 tested primers showed consistent banding patterns. Two groups with a similarity level above 60% were observed in the analysis profiles and one of them formed only by isolates from the lesser mealworm, with 72% of similarity. Was found relationship between the origin host of the isolates and the molecular profile, generated in the analysis. There was no relationship between the molecular profiles and the geographical location or pathogenicity to the insect. The results showed that even the isolates collected in near areas may have highly variability.

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Author Biographies

Daian Guilherme Pinto de Oliveira, Universidade Federal da Fronteira Sul - UFFS

Mestre em Entomologia pela Escola Superior de Agricultura 'Luiz de Queiroz', ESALQ. Doutorando em Entomologia pela Universidade Federal do Paraná, UFPR. Professor Assistente na Universidade Federal da Fronteira Sul - UFFS

Fabiana Gisele da Silva Pinto, Universidade Estadual do Oeste do Paraná

Doutora em Microbiologia, UEL. Professora e pesquisadora na Universidade Estadual do Oeste do Paraná, UNIOESTE - Cascavel, PR, Centro de Ciências Biológicas e da Saúde.

Fernando Gomes Barcellos, Faculdade Arthur Sá Earp Neto

Doutor em Agronomia, USP. Professor na Faculdade Arthur Sá Earp Neto, Petrópolis, RJ.

Luis Francisco Angeli Alves, Universidade Estadual do Oeste do Paraná

Doutor em Entomologia, ESALQ, Professor e pesquisador na Universidade Estadual do Oeste do Paraná, UNIOESTE - Cascavel, PR. Centro de Ciências Biológicas e da Saúde.

Mariangela Hungria, Embrapa Soja

Pós-Doutora Pesquisadora da Embrapa Soja, Londrina, PR. Laboratório de Biotecnologia dos Solos.

Published

2011-03-31

How to Cite

Oliveira, D. G. P. de, Pinto, F. G. da S., Barcellos, F. G., Alves, L. F. A., & Hungria, M. (2011). Genetic variability of the fungus Beauveria spp. isolates and virulence to the lesser mealworm Alphitobius diaperinus Panzer (Coleoptera: Tenebrionidae). Semina: Ciências Agrárias, 32(1), 147–156. https://doi.org/10.5433/1679-0359.2011v32n1p147

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