Synchronization with estradiol benzoate in the presence of the corpus luteum in Wagyu cows increases the number of medium follicles but does not interfere with in vitro production of embryos
DOI:
https://doi.org/10.5433/1679-0359.2021v42n3p1147Keywords:
Reproduction biotechnology, Wagyu donors, Follicular aspiration, Follicular synchronization.Abstract
Reproductive biotechnologies emerge as an important element for livestock, however, some strategies must be modified to adapt the different breeding systems, such as the use of follicular synchronization protocols. This study aimed to evaluate follicular synchronization using estradiol benzoate, with the presence of the corpus luteum, on the in vitro embryo production (IVEP) from Wagyu oocytes donors. Routines of IVEP were performed in heifers and cows (n=19), classified into three groups: G1/CL- animals with corpus luteum; G2/SCL- animals without corpus luteum, these groups being the control, and had the natural process of follicular dynamics and G3/CL + EB - animals with the presence of corpus luteum, submitted to follicular synchronization at D0, with estradiol benzoate. The results obtained showed that the synchronization of the follicular wave with the application of EB, in the presence of the corpus luteum (CL), reduced the number of small (6.05 ± 0.55) and large follicles (0.45 ± 0.15), but increased (P<0.05) the amount of medium follicles (16.20 ± 0.90). However, the results of OPU showed that the number of viable oocytes and the viability rate, depending on the application or not of EB and the presence or not of CL in Wagyu donor, did not differ between groups (P>0.05). It was concluded that follicular synchronization using estradiol benzoate in Wagyu oocyte donors, presenting a corpus luteum, increased the number of medium follicles, however, there was no improvement in the efficiency of ovum pick-up, in vitro embryo production and pregnancy rate.
Downloads
References
Almeida, M. D. O. B., Braga, H., Fernandes, M. B., Sotomaior, C. S., & Pereira, J. F. S. (2016). Comparação da Produção in vitro de Embriões nas Raças Red Angus e Aberdeen Angus. Revista Eletrônica Biociências, Biotecnologia e Saúde, 6(15), 235-236.
Associação Brasileira das Indústrias Exportadoras de Carnes (2018). Perfil da pecuária no Brasil. (Relatório Anual, 2017). São Paulo: ABIEC.
Bacelar, D., Max, M. C., Padilha, L. C., Barreiros, T. R. R., & Seneda, M. M. (2010). Incremento na obtenção de oócitos em novilhas Nelore (Bos taurus indicus) tratadas com progesterona injetável e benzoato de estradiol. Semina: Ciências Agrárias, 31(1), 163-171.
Bó, G. A., Cedeño, A., & Mapletoft, R. J. (2019). Strategies to increment in vivo and in vitro embryo production and transfer in cattle. Animal Reproduction, 16(3), 411-422. doi: 10.21451/1984-3143-AR 2019-0042
Carvalho, T. B., & De Zen, S. (2017). A cadeia de pecuária de corte no Brasil: evolução e tendências. Revista iPecege, 3(1), 85-99. doi: 10.22167/r.ipecege.2017.1.85
Cavalieri, F. L. B., Morotti, F., Seneda, M. M., Colombo, A. H. B., Andreazzi, M. A., Emanuelli, I. P., & Rigolon, L. P. (2018). Improvement of bovine in vitro embryo production by ovarian follicular wave synchronization prior to ovum pick-up. Theriogenology, 117(1), 57-60. doi: 10.1016/j.theriogenology. 2017.11.026
Colombo, A. H. B., Cavalieri, F. L. B., Andreazzi, M. A., Moreski, D. A. B., Arruda Botelho, A. C. F. de, & Santos, J. M. G. dos. (2017). Avaliação de biotécnicas da reprodução sob o foco ambiental. Archives of Veterinary Science, 22(1), 81-89. doi: 10.5380/avs.v22i1.50814
De Loos, F., Van Vliet, C., Van Maurik, P., & Kruip, T. A. (1989). Morphology of immature bovine oocytes. Gamete research, 24(2), 197-204. doi: 10.1002/mrd.1120240207
Ferreira, A. D. M., Viana, J. H. M., Camargo, L. S. D. A., Sá, W. F. D., & Pereira, P. A. C. (2004). População folicular ovariana durante o ciclo estral em vacas da raça Gir. Revista Brasileira de Zootecnia, 33(6), 1689-1694. doi: 10.1590/S1516-35982004000700007
Gimenes, L. U., Ferraz, M. L., Fantinato, P. Neto, Chiaratti, M. R., Mesquita, L.G., Sá, M .F. Fo.,… Baruselli, P. S. (2015). The interval between the emergence of pharmacologically synchronized ovarian follicular waves and ovum pickup does not significantly affect in vitro embryo production in Bos indicus, Bos taurus, and Bubalus bubalis. Theriogenology, 83(3), 385-93. doi: 10.1016/j.theriogenology. 2014.09.030
Hidaka, T., Fukumoto, Y., Yamamoto, Y., Ogata, Y., & Horiuchi, T. (2018). Estradiol benzoate treatment before ovum pick-up increases the number of good quality oocytes retrieved and improves the production of transferable embryos in Japanese Black cattle. Veterinary and Animal Science, 5(1), 1-6. doi: 10.1016/j.vas.2018.02.001
Lustosa, A. A., Barboza, N. A., Barbosa, Y. G. D. S., Rodrigues, P. K. O., & Magalhães, F. D. C. R. Neto, (2018). Aspectos relevantes na produção comercial de embriões bovinos por meio da técnica biotecnológica de fertilização in vitro: Revisão. PUBVET, 12(3), 130. doi: 10.22256/pubvet.v12n3a51. 1-6
Machatkova, M., Jokesova, E., Horky, F., & Krepelova, A. (2000). Utilization of the growth phase of the first follicular wave for bovine oocyte collection improves blastocyst production. Theriogenology, 54(4), 543-550. doi: 10.1016/S0093-691X(00)00369-1
Mello, R. R. C., Ferreira, J. E., Sousa, S. L. G. D., Mello, M. R. B. D., & Palhano, H. B. (2016). Produção in vitro (PIV) de embriões em bovinos. Revista Brasileira de Reprodução Animal, 40(2), 6458-6458.
Ongaratto, F. L., Rodriguez-Villamil, P., Tribulo, A., & Bó, G. A. (2015). Effect of follicle wave synchronization and gonadotropin treatments on the number and quality of cumulus-oocyte complex obtained by ultrasound-guided ovum pick-up in beef cattle. Animal Reproduction, 12(4), 876-883.
Ramos, A. F., Rumpf, R., Câmara, J. U., Mollo, M. R., Pivato, I., Marques, A. P., Jr., & Sartori, R. (2010). Effect of follicular wave synchronization on in vitro embryo production in heifers. Animal reproduction science, 117(3-4), 201-207. doi: 10.1016/j.anireprosci.2009.04.009
Seneda, M. M., Zangirolamo, A. F., Bergamo, L. Z., & Morotti, F. (2020). Follicular wave synchronization prior to ovum pick-up. Theriogenology, 150(1), 180-185. doi: 10.1016/bs.agron.2016.10.002
Silva, N. C., Sanches, T. K., Morotti, F., Zangirolamo, A. F., Orsi, M. L., & Seneda, M. M. (2019). Influência da fase de atividade ovariana sobre a produção in vitro de embriões bovinos. Anais da Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Ilha de Comandatuba, BA, Brasil, 33.
Simplício, A. P. G., & Pierre, F. C. (2018). Genetic improvement: use of visual score in cutting livestock. Tekhne e Logos, 9(2), 36-47.
Statistical Analyses System (2000). SAS. Versão 8,0. Cary, North Carolina: The SAS Institute.
Taninaka, T., Bernardino, T., Meneghini, R., & Santos, G. dos. (2015). Análise da viabilidade econômica de um rebanho de gado de corte da raça Wagyu em ciclo completo. Revista iPecege, 1(2), 44-58. doi: 10. 22167/r.ipecege.2015.2.44. 44
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Semina: Ciências Agrárias
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Semina: Ciências Agrárias adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.
