Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation medium
DOI:
https://doi.org/10.5433/1679-0359.2020v41n6Supl2p3155Keywords:
Corneal transplantation. Horses, Endothelium, Corneal storage, Cell viability.Abstract
The objective of this study was evaluate the maintenance of the corneal endothelium of horses in cold EUSOL-C® preservation medium over different periods (seven and 14 days) using scanning electron microscopy. A total of 20 pairs of eyes from horses were analysed. The corneas were divided into four groups of 10 corneas each (G1, G2, G3 and G4): G1 - the samples were kept in the preservation medium for seven days; G3 - the samples were kept in the preservation medium for for 14 days; G2 and G4 were formed by the control corneal buttons of G1 and G3, respectively. The average cell loss observed in G1 was 7.62%, in G2 it was 7.04%, in G3 9.12% and in G4 7.16%. No statistically significant differences were observed between the four groups. It was concluded that the Eusol-C® hypothermic preservation medium provided satisfactory preservation of the corneal endothelium in equine species for up to 14 days.Downloads
Download data is not yet available.
References
Abib, F. C., & Barreto, J. R. (2001). Behavior of corneal endotelial density over a lifetime. Journal of Cataract and Refractive Surgery, 27(10), 1574-1578. doi: 10.1016/s0886-3350(01)00925-7
Andrew, S. E., Ramsey, D. T., Hauptman, J. G., & Brooks, D. E. (2001). Density of corneal endothelial cells and corneal thickness in eyes of euthanatized horses. American Journal of Veterinay Research, 62(4), 479-482. doi: 10.2460/ajvr.2001.62.479
Brooks, D. E., Plumer, C. E., Kallberg, M. E., Barrie, K. P., Ollivier, F. J., Hendrix, D. V., & Gelatt K. N. (2008). Corneal transplantation for inflammatory keratopathies in the horse: visual outcome in 206 cases (1993-2007). Veterinary Ophthalmology, 11(2), 123-133. doi: 10.1111/j.1463-5224.2008.00611.x
Cichocki, B. M., Myrna, K. E., & Moore, P. A. (2016). Modified penetrating keratoplasty with Acell® bioscaffold implant in seven horses with deep full-thickness corneal stromal abscess. Veterinary Ophthalmology, 20(1), 46-52. doi: 10.1111/vop.12349
Collin, S. P., & Collin, H. B. (1998). A comparative study of the corneal endothelium in vertebrates. Clinical Experimental Opthalmology, 81(6), 245-254. doi: 10.1111/j.1444-0938.1998.tb06744.x
Denis, H. M. (2004). Equine corneal surgery and transplantation. The Veterinary Clinics of North American Equine Practice, 20(2), 361-380. doi: 10.1016/j.cveq.2004.04.012
Feilmeier, M. R., Tabin, G. C., Williams, L., & Oliva, M. (2010). The use of glycerol-preserved corneas in the developing world. Midle East African Journal Ophthalmology, 17(1), 38-43. doi: 10.4103/0974-9233.61215
Gain, P., Jullienne, R., He, Z., Aldossary, M., Acquart, S., Cognasse, F., & Thuret, G. (2016). Global survey of corneal transplantation and eye banking. JAMA Ophthalmology, 134(2), 167-173. doi: 10.1001/ jamaophthalmol.2015.4776
Hwang, H., & Kim, M. (2009). Endothelial damage of a donor cornea depending on the donor insertion method during Descemet-stripping automated endothelial keratoplasty in porcine eyes. Japanese Journal of Ophthalmology, 53(5), 523-530. doi: 10. 1007/s10384-009-0713-4
Joyce, N. C. (2012). Proliferative capacity of corneal endothelial cells. Experimental Eye Research, 95(1), 16-23. doi: 10.1016/j.exer.2011.08.014
Kanavi, M. R., Javadi, M. A., Chamani, T., Fahim, P., & Javadi, F. (2014). Comparing quantitative and qualitative índices of the donated corneas maintained in Optisol-GS with those kept in Eusol-C. Cell and Tissue Banking, 16(2), 243-247. doi: 10.1007/s10561-014-9466-5
Kryczka, T., Chrapusta, S. J., Szaflik, J. P., Szaflik, J., & Midelfart, A. (2014). Impact of donor health on corneal biochemistryan unexpected caveat from a pilot study. Annals of Transplantation, 12(19), 129-137. doi: 10.12659/AOT.890215
Lambert, N. G., & Chamberlain, W. D. (2017). The structure and evolution of eye banking: a review on eye banks’ historical, present, and future contribution to corneal transplantation. Journal of Biorepository Science for Applied Medicine, 5(17), 23-40. doi: 10. 2147/BSAM. S114197
Lira, F. J. X., & Sá, F. B. (2012). Uso do meio Roswell Park Memorial Institute como conservante de córneas de camundongos Swiss. Medicina Veterinária, 6(2), 18-25. doi: 10.1007/s46561876-J486-5
Means, T. L., Geroski, D. H., Hadley, A., Lynn, M. J., & Edelhauser, H. F. (1995). Viability of human corneal endothelium following Optisol-GS storage. Archives of Ophthalmology, 113(6), 805-809. doi: 10.1001/archopht.1995.01100060131047
Peels, L. (1997). Organ culture: the method of choice for preservation of human donor corneas. Britsh Journal of Ophthalmology, 81(7), 523-525. doi. 10.1136/bjo.81.7.523
Pigatto, J, A. T., Abib, F. C., Pizetti, J. C., Laus, J. L., Santos, J. M. & Barros, P. S. M. (2005a). Morphometric analysis of the corneal endothelium of rabbits using scanning electron microscopy. Acta Scientiae Veterinarie, 33(1), 41-45. doi: 10.22456/1679-9216.14441
Pigatto, J. A. T., Andrade, M. C., Laus, J. L., Santos, J. M., Brooks, D. E., Guedes, P. M., & Barros, P. S. M. (2004). Morphometric analysis of the corneal endothelium of Yacare caiman (Caiman yacare) using scanning electron microscopy. Veterinary Ophthalmology, 7(3), 205-208. doi: 10.1111/j.1463-5224. 2004.04025.x
Pigatto, J. A. T., Franzen, A. A., Pereira, F. Q., Almeida, A. C. da V. R., Laus, J. L., Santos, J. M., Guedes, P. M., & Barros, P. S. M. (2009). Scanning electron microscopy of the corneal endothelium of ostrich. Ciência Rural, 39(3), 926-929. doi: 10.1590/S0103-84782009005000001
Pigatto, J. A. T., Laus, J. L., Santos, J. M., Cerva, C., Cunha, L. S., Ruoppolo, V., & Barros, P. S. M. (2005b). Corneal endothelium of the Magellanic penguin (Spheniscus magellanicus) by scanning electron microscopy. Journal of Zoo and Wildlife Medicine, 36(4), 702-705. doi: 10.1638/05017.1
Plumer, C. E. (2009). The use of amniotic membrane transplantation for ocular surface reconstruction: a review and series of 58 equine clinical cases (2002-2008). Veterinary Ophthalmology, 12(1), 17-24. doi: 10.1111/j.1463-5224.2009.00741.x
Rodrigues, G. N., Laus, J. L., Santos, J. M., Rigueiro, M. P., & Smith, R. L. (2006). Corneal endothelial cell morphology of normal dogs in different ages. Veterinary Ophthalmology, 9(2), 101-107. doi: 10.1111/j. 1463-5224.2006.00427.x
Rosenwasser, G. O., Szczotka-Flynn, L. B., Ayala, A. R., Liang, W., Aldave, A. J., Dunn, S. P.,… Lass, J. H. (2017). Effect of cornea preservation time on success of descemet stripping automated endothelial keratoplasty. JAMA Ophthalmology, 135(12), 1401-1409. doi: 10.1001/jamaophthalmol.2017.4989
Tamayo-Arango, L. J., Baraldi-Artoni, S. M., Laus, J. L., Mendes-Vicenti, F. A., Pigatto, J. A. T., & Abib, F. C. (2009). Ultrastructural morphology and morphometry of the normal corneal endothelium of adult crossbred pig. Ciencia Rural, 39(1), 117-122. doi: 10.1590/S0103-84782009000100018
Tan, D. T., Dart, J. K., Holland, E. J., & Kinoshita, S. (2012). Corneal transplantation. Lancet, 379(9827), 1749-1761. doi: 10.1016/S0140-6736(12)60437-1
Terry, M. A., Hoar, K. L., Wall, J., & Ousley, P. (2006). Histology of dislocations in endothelial keratoplasty (DSEK and DLEK): a laboratory-based, surgical solution to dislocation in 100 consecutive DSEK cases. Cornea, 25(8), 926-932. doi: 10.1097/01.ico.0000243958.07027.f2
Terzariol, M., Hünning, P. S., Brambatti, G., Albuquerque, L., Neumann, C., & Pigatto, J. A. T. (2016). Avaliação do endotélio corneano suíno por microscopia eletrônica de varredura após aplicação de azul brilhante a 0, 05 % na câmara anterior: estudo in vitro. Pesquisa Veterinária Brasileira, 36(8), 775-780. doi: 10.1590/S0100-736X2016000800016
Villarrubia, A., García, E., Cano-Ortiz, A., Membrillo, A., Sabala, A., Celis, J., & Sassot, I. (2017). Scanning electron microscope analysis of stromal surface regularity in DSAEK using manual or automated microkeratomes or DMEK. Cornea, 36(5), 588-593. doi: 10.1097/ICO.0000000000001135
Wagoner, M. D., & Gonnah, E. S. (2005). Corneal graft survival after prolonged storage in Optisol-GS. Cornea, 24(8), 976-979. doi: 10.1097/01.ico.0000159731.52801.b6
Yüksel, B., Uzunel, U. D., & Küsbeci, T. (2016). Endothelial cell viability of donor corneas preserved in Eusol-C corneal storage medium. Experimental Clinicals Transplant, 14(4), 441-444. doi: 10.6002/ect. 2014.0295
Andrew, S. E., Ramsey, D. T., Hauptman, J. G., & Brooks, D. E. (2001). Density of corneal endothelial cells and corneal thickness in eyes of euthanatized horses. American Journal of Veterinay Research, 62(4), 479-482. doi: 10.2460/ajvr.2001.62.479
Brooks, D. E., Plumer, C. E., Kallberg, M. E., Barrie, K. P., Ollivier, F. J., Hendrix, D. V., & Gelatt K. N. (2008). Corneal transplantation for inflammatory keratopathies in the horse: visual outcome in 206 cases (1993-2007). Veterinary Ophthalmology, 11(2), 123-133. doi: 10.1111/j.1463-5224.2008.00611.x
Cichocki, B. M., Myrna, K. E., & Moore, P. A. (2016). Modified penetrating keratoplasty with Acell® bioscaffold implant in seven horses with deep full-thickness corneal stromal abscess. Veterinary Ophthalmology, 20(1), 46-52. doi: 10.1111/vop.12349
Collin, S. P., & Collin, H. B. (1998). A comparative study of the corneal endothelium in vertebrates. Clinical Experimental Opthalmology, 81(6), 245-254. doi: 10.1111/j.1444-0938.1998.tb06744.x
Denis, H. M. (2004). Equine corneal surgery and transplantation. The Veterinary Clinics of North American Equine Practice, 20(2), 361-380. doi: 10.1016/j.cveq.2004.04.012
Feilmeier, M. R., Tabin, G. C., Williams, L., & Oliva, M. (2010). The use of glycerol-preserved corneas in the developing world. Midle East African Journal Ophthalmology, 17(1), 38-43. doi: 10.4103/0974-9233.61215
Gain, P., Jullienne, R., He, Z., Aldossary, M., Acquart, S., Cognasse, F., & Thuret, G. (2016). Global survey of corneal transplantation and eye banking. JAMA Ophthalmology, 134(2), 167-173. doi: 10.1001/ jamaophthalmol.2015.4776
Hwang, H., & Kim, M. (2009). Endothelial damage of a donor cornea depending on the donor insertion method during Descemet-stripping automated endothelial keratoplasty in porcine eyes. Japanese Journal of Ophthalmology, 53(5), 523-530. doi: 10. 1007/s10384-009-0713-4
Joyce, N. C. (2012). Proliferative capacity of corneal endothelial cells. Experimental Eye Research, 95(1), 16-23. doi: 10.1016/j.exer.2011.08.014
Kanavi, M. R., Javadi, M. A., Chamani, T., Fahim, P., & Javadi, F. (2014). Comparing quantitative and qualitative índices of the donated corneas maintained in Optisol-GS with those kept in Eusol-C. Cell and Tissue Banking, 16(2), 243-247. doi: 10.1007/s10561-014-9466-5
Kryczka, T., Chrapusta, S. J., Szaflik, J. P., Szaflik, J., & Midelfart, A. (2014). Impact of donor health on corneal biochemistryan unexpected caveat from a pilot study. Annals of Transplantation, 12(19), 129-137. doi: 10.12659/AOT.890215
Lambert, N. G., & Chamberlain, W. D. (2017). The structure and evolution of eye banking: a review on eye banks’ historical, present, and future contribution to corneal transplantation. Journal of Biorepository Science for Applied Medicine, 5(17), 23-40. doi: 10. 2147/BSAM. S114197
Lira, F. J. X., & Sá, F. B. (2012). Uso do meio Roswell Park Memorial Institute como conservante de córneas de camundongos Swiss. Medicina Veterinária, 6(2), 18-25. doi: 10.1007/s46561876-J486-5
Means, T. L., Geroski, D. H., Hadley, A., Lynn, M. J., & Edelhauser, H. F. (1995). Viability of human corneal endothelium following Optisol-GS storage. Archives of Ophthalmology, 113(6), 805-809. doi: 10.1001/archopht.1995.01100060131047
Peels, L. (1997). Organ culture: the method of choice for preservation of human donor corneas. Britsh Journal of Ophthalmology, 81(7), 523-525. doi. 10.1136/bjo.81.7.523
Pigatto, J, A. T., Abib, F. C., Pizetti, J. C., Laus, J. L., Santos, J. M. & Barros, P. S. M. (2005a). Morphometric analysis of the corneal endothelium of rabbits using scanning electron microscopy. Acta Scientiae Veterinarie, 33(1), 41-45. doi: 10.22456/1679-9216.14441
Pigatto, J. A. T., Andrade, M. C., Laus, J. L., Santos, J. M., Brooks, D. E., Guedes, P. M., & Barros, P. S. M. (2004). Morphometric analysis of the corneal endothelium of Yacare caiman (Caiman yacare) using scanning electron microscopy. Veterinary Ophthalmology, 7(3), 205-208. doi: 10.1111/j.1463-5224. 2004.04025.x
Pigatto, J. A. T., Franzen, A. A., Pereira, F. Q., Almeida, A. C. da V. R., Laus, J. L., Santos, J. M., Guedes, P. M., & Barros, P. S. M. (2009). Scanning electron microscopy of the corneal endothelium of ostrich. Ciência Rural, 39(3), 926-929. doi: 10.1590/S0103-84782009005000001
Pigatto, J. A. T., Laus, J. L., Santos, J. M., Cerva, C., Cunha, L. S., Ruoppolo, V., & Barros, P. S. M. (2005b). Corneal endothelium of the Magellanic penguin (Spheniscus magellanicus) by scanning electron microscopy. Journal of Zoo and Wildlife Medicine, 36(4), 702-705. doi: 10.1638/05017.1
Plumer, C. E. (2009). The use of amniotic membrane transplantation for ocular surface reconstruction: a review and series of 58 equine clinical cases (2002-2008). Veterinary Ophthalmology, 12(1), 17-24. doi: 10.1111/j.1463-5224.2009.00741.x
Rodrigues, G. N., Laus, J. L., Santos, J. M., Rigueiro, M. P., & Smith, R. L. (2006). Corneal endothelial cell morphology of normal dogs in different ages. Veterinary Ophthalmology, 9(2), 101-107. doi: 10.1111/j. 1463-5224.2006.00427.x
Rosenwasser, G. O., Szczotka-Flynn, L. B., Ayala, A. R., Liang, W., Aldave, A. J., Dunn, S. P.,… Lass, J. H. (2017). Effect of cornea preservation time on success of descemet stripping automated endothelial keratoplasty. JAMA Ophthalmology, 135(12), 1401-1409. doi: 10.1001/jamaophthalmol.2017.4989
Tamayo-Arango, L. J., Baraldi-Artoni, S. M., Laus, J. L., Mendes-Vicenti, F. A., Pigatto, J. A. T., & Abib, F. C. (2009). Ultrastructural morphology and morphometry of the normal corneal endothelium of adult crossbred pig. Ciencia Rural, 39(1), 117-122. doi: 10.1590/S0103-84782009000100018
Tan, D. T., Dart, J. K., Holland, E. J., & Kinoshita, S. (2012). Corneal transplantation. Lancet, 379(9827), 1749-1761. doi: 10.1016/S0140-6736(12)60437-1
Terry, M. A., Hoar, K. L., Wall, J., & Ousley, P. (2006). Histology of dislocations in endothelial keratoplasty (DSEK and DLEK): a laboratory-based, surgical solution to dislocation in 100 consecutive DSEK cases. Cornea, 25(8), 926-932. doi: 10.1097/01.ico.0000243958.07027.f2
Terzariol, M., Hünning, P. S., Brambatti, G., Albuquerque, L., Neumann, C., & Pigatto, J. A. T. (2016). Avaliação do endotélio corneano suíno por microscopia eletrônica de varredura após aplicação de azul brilhante a 0, 05 % na câmara anterior: estudo in vitro. Pesquisa Veterinária Brasileira, 36(8), 775-780. doi: 10.1590/S0100-736X2016000800016
Villarrubia, A., García, E., Cano-Ortiz, A., Membrillo, A., Sabala, A., Celis, J., & Sassot, I. (2017). Scanning electron microscope analysis of stromal surface regularity in DSAEK using manual or automated microkeratomes or DMEK. Cornea, 36(5), 588-593. doi: 10.1097/ICO.0000000000001135
Wagoner, M. D., & Gonnah, E. S. (2005). Corneal graft survival after prolonged storage in Optisol-GS. Cornea, 24(8), 976-979. doi: 10.1097/01.ico.0000159731.52801.b6
Yüksel, B., Uzunel, U. D., & Küsbeci, T. (2016). Endothelial cell viability of donor corneas preserved in Eusol-C corneal storage medium. Experimental Clinicals Transplant, 14(4), 441-444. doi: 10.6002/ect. 2014.0295
Downloads
Published
2020-11-06
How to Cite
Albuquerque, L. de, Pigatto, A. M., & Pigatto, J. A. T. (2020). Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation medium. Semina: Ciências Agrárias, 41(6Supl2), 3155–3164. https://doi.org/10.5433/1679-0359.2020v41n6Supl2p3155
Issue
Section
Articles
License
Copyright (c) 2020 Semina: Ciências Agrárias
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Semina: Ciências Agrárias adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.
