Validation of a diagrammatic scale for quantification of bacterial leaf blight (causal agent Pseudomonas avenae) in maize
DOI:
https://doi.org/10.5433/1679-0359.2020v41n6p2467Keywords:
Disease assessment, Pathometry, Zea mays L.Abstract
Bacterial leaf blight occurs in almost the entire Brazilian territory and can cause significant economic damage. However, its effects can be curtailed with the following tools that aid in quantification for carrying out the best severity assessment that can be applied to the studies used in its control. The objective of this study was to develop and validate a diagrammatic scale to quantify the severity of bacterial leaf blight in maize caused by Pseudomonas avenae. Corn leaves were collected with different levels of disease severity, and the total leaf area as well as the injured area of the middle third of each leaf was measured. From this, the minimum and maximum limits of the disease and, subsequently, four intermediate levels were determined whilst taking into account the law of visual stimulus of Weber-Fechner. Levels 1.5%, 2.5%, 9%, 18%, 46%, and 70% were selected with validation performed by eight evaluators, four inexperienced and four experienced. These evaluators estimated the severity values, initially without the use of the proposed diagrammatic scale and in a second attempt with the use of the scale. We concluded that it was possible to improve the accuracy and precision of the evaluators when they used the scale as a tool to assess the severity of bacterial leaf blight on corn leaves.Downloads
References
Alves, G. C. S., Santos, L. C., Duarte, H. S. S., Dias, V., Zambolim, L., & Rocha, M. R. (2015). Escala diagramática para quantificação da ferrugem da folha do trigo. Multi-Science Journal, 1(1), 128-133. doi: 10.33837/msj.v1i1.59 Recuperado de https://www.ifgoiano.edu.br/periodicos/index.php/ multiscience/article/view/59
Amorim, L., & Bergamin, A. F. (2018). Fenologia, patometria e quantificação de danos. In L. Amorin, J. A. M. Rezende, & A. Bergamin, Fo. (Eds.), Manual de fitopatologia: princípios de conceitos (pp. 499- 518). Ouro Fino, MG: Ceres.
Berger, R. D. (1980). Measuring disease intensity. In P. S., Teng, & S. V. Krupa (Eds.), Crop loss assessment (pp. 28-31). Saint Paul, Minnesota: University of Minnesota.
Carvalho, R. V., Pereira, O. A. P., & Camargo, L. E. A. (2016). Doenças do milho. In L, Amorim, J. A. M. Rezende, A. Bergamin Fo, & L. E. A Camargo (Eds.), Manual de fitopatologia: doenças das plantas cultivadas (pp. 549-560). Ouro Fino, MG: Ceres.
Cruz, C. D. (2013). Genes: a software package for analysis in experimental statistics and quantitative genetics. Acta Scientiarum. Agronomy, 35(3), 271-276. doi: 10.4025/actasciagron.v35i3.21251 Retrieved from http://www.scielo.br/pdf/asagr/v35n3/v35n3a01.pdf
Horsfall, J. C., & Barratt, R. W. (1945). An improved grading system for measuring plant diseases. Phytopathology, 35, 665-665. Retrieved from http://www.garfield.library.upenn.edu/classics1986/ A1986A666500001.pdf
Lanza, F. E., Zambolim, L., Costa, R. V., Silva, D. D., Queiroz, V. A. V., Parreira, D. F., Cota, L. V. (2016). Aplicação foliar de fungicidas e incidência de grãos ardidos e fumonisinas totais em milho. Pesquisa Agropecuária Brasileira, 51(5), 638-646. doi: 10.1590/S0100-204X2016000500026 Recuperado de https://ainfo.cnptia.embrapa.br/digital/bitstream/item/146150/1/Aplicacao-foliar-de-fungicidas.pdf
Lazaroto, A., Santos, I., Konflanz, V., Malagi, G., & Camochena, R. C. (2012). Escala diagramática para avaliação de severidade da helmintosporiose comum em milho. Ciência Rural, 42(12), 2131-2137. doi: 10.1590/S0103-84782012005000112 Recuperado de http://www.scielo.br/pdf/cr/v42n12/a34912cr 6772.pdf
Lenz, G., Balardin, R. S., Corte, G. D., Marques, L. N., & Debona, D. (2010). Escala diagramática para avaliação de severidade de mancha-parda em arroz. Ciência Rural, 40(4), 752-758. doi: 10.1590/S0103-84782010005000061 Recuperado de http://www.scielo.br/pdf/cr/v40n4/a539cr2706.pdf
Mafia, R. G., Ferreira, M. A., Binoti, D. H. B., & Leite, H. G. (2011). Procedimento estatístico para validação de escalas diagramáticas na quantificação de doenças. Revista Árvore, 35(2), 199-204. doi: 10.1590/S0100-67622011000200004 Recuperado de http://www.scielo.br/pdf/rarv/v35n2/a04v35n2.pdf
Nunes, C. C., & Alves, S. A. M. (2012). Elaboração e validação de escala diagramática para quantificação da severidade de entomosporiose em folhas de pereira. Summa Phytopathologica, 38(3), 239-244. doi: 10.1590/S0100-54052012000300011 Recuperado de http://www.scielo.br/pdf/sp/v38n3/a11v38n3.pdf
Nutter, F. W., Jr., & Schultz, P. M. (1995). Improving the accuracy and precision of disease assessments: selection of methods and use of computer-aided training programs. Canadian Journal of Plant Pathology, 17(2), 174-184. doi: 10.1080/07060669509500709 Retrieved from https://www.tandfonline. com/doi/abs/10.1080/07060669509500709
Sachs, P. D., Neves, C. J., Canteri, M. G., & Sachs, L. G. (2011). Escala diagramática para avaliação da severidade da mancha branca em milho. Summa Phytopathologica, 37(4), 202-204. doi: 10.1590/S0100-54052011000400007 Recuperado de http://www.scielo.br/pdf/sp/v37n4/a07v37n4.pdf
Sherwood, R. T., Berg, C. C., Hoover, M. R., & Zeiders, K. E. (1983). Illusions in visual assessment of stagonospora leaf spot of orchardgrass. Phytopathology, 73(2), 173-177. doi: 10.1094/phyto-73-173 Retrieved from https://www.apsnet.org/publications/phytopathology/backissues/Documents/1983 Articles/Phyto73n02_173.PDF
Trojan, D. G., & Pria, M. D. (2018). Validação de escala diagramática para quantificação da severidade da antracnose da folha do milho. Summa Phytopathologica, 44(1), 56-64. doi: 10.1590/0100-5405/172675 Recuperado de http://www.scielo.br/pdf/sp/v44n1/0100-5405-sp-44-1-0056.pdf
Vale, F. X. R., Fernandes, E. I. F., Fº., & Liberato, J. R. (2001). QUANT: software to quantify plant disease severity. Proceedings of the International Workshop on Plant Disease Epidemiology, Ouro Preto, MG, Brazil, 8.
Vivas, M., Terra, C. E. P. S., Silveira, S. F., Fontes, R. V., & Pereira, M. G. (2010). Escala diagramática para avaliação da severidade de pinta-preta em frutos de mamoeiro. Summa Phytopathologica, 36(2), 161-163. doi: 10.1590/0100-5405/178543 Recuperado de http://www.scielo.br/pdf/sp/v44n3/0100-5405-sp-44-3-0252.pdf
White, D. G. (1999). Compendium of corn diseases. Saint Paul, Mineesota: American Phytopathological Society.
Willems, A., Goor, M., Thielemans, S., Gillis, M., Kersters, K. E., & Ley, J. (1992). Transfer of several phytopathogenic Pseudomonas species to Acidovorax avenae subsp. avenae subsp. nov. comb. nov., Acidovorax avenae subsp. citrulli, Acidovorax avenae subsp. cattleyae, and Acidovorax konjaci. International Journal of Systematic Bacteriology, 42(1), 107-119. doi: 0020-7713/92/010107-13$02 https://www.microbiologyresearch.org/docserver/fulltext/ijsem/42/1/ijs-42-1-107.pdf?expires=15593 10 202&id=id&accname=guest&checksum=6A0EBA5B39E3FFD2E611A4B9D5EF4B67
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 Semina: Ciências Agrárias
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Semina: Ciências Agrárias adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.