Humoral immune response in beef heifers supplemented with mineral salt with or without the addition of rumen-protected methionine
DOI:
https://doi.org/10.5433/1679-0359.2019v40n6Supl2p3057Keywords:
Amino acids, Pampa biome, Immunity, Mineralization.Abstract
The aim of this study was to evaluate the humoral immune response in beef heifers supplemented with mineral supplementation with or without the addition of rumen-protected methionine. Forty-eight Brangus nulliparous heifers were distributed into four experimental groups with three replications each: control group without supplementation and without vaccination (CG01), control group without supplementation and with vaccination (CG02), treatment group with mineral supplementation and vaccination (TG01), and treatment group with mineral supplementation added with protected methionine and vaccination (TG02). The animals were maintained under native pasture with access to water ad libitum and the supplementation was available in high-consumption covered troughs. A supplementation period of 60 days prior to vaccinations was adopted until the first dose of a monovalent experimental vaccine inactivated for BoHV-5 was applied as a method of stimulating the immune response to evaluate the supplementation effects. After a 21-day interval, blood samples were collected to evaluate the humoral response and the second vaccine booster dose was applied following the 21-day interval for new blood samples in order to evaluate the immune response against the two-vaccination protocol. From the beginning of the experiment, the animals were weighed on the days ?60, ?10, 0, 21, and 42 in relation to the vaccine protocol. The experimental groups did not differ for body weight, mean daily weight gain, and body condition score after 102 days of supplementation regardless of the treatment. No animals belonging to CG01 seroconverted throughout the experiment, proving that there was no introduction of the agent (BoHV) in the studied area. When vaccinated animals were compared to the CG01 control group, statistically higher levels of neutralizing antibodies (P ? 0.0001) and IgG (P ? 0.0001) were verified 21 days after the second vaccine dose. Among the animals of the three vaccinated groups, there was no difference in seroconversion and IgG production. Therefore, no benefits of mineral supplementation or enriched with protected methionine were observed for the humoral immune response of the studied animals.Downloads
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References
ARAUJO, I. L.; DUMMER, L. A.; RODRIGUES, P. R. C.; SANTOS JUNIOR, A. G. dos; FISCHER, G.; CUNHA, R. C.; LEITE, F. P. L. Immune responses in bovines to recombinant glycoprotein D of bovine herpesvirus type 5 as vaccine antigen. Vaccine, Kidlington, v. 36, n. 50, p. 7708-7714, 2018. DOI: 10.1016/j.vaccine.2018.10.080
CERONE, S. I.; SANSINANEA, A. S.; AUZA, N. J. Copper deficiency alters the immune response of bovine. Nutrition Research, New York, v. 15, n. 9, p. 1333-1341, 1995. DOI: 10.1016/0271-5317(95)02008-J
CLEMENTS, A. R.; IRELAND, F. A.; FREITAS, T.; TUCKER, H.; SHIKE, D. W. Effects of supplementing methionine hydroxy analog on beef cow performance, milk production, reproduction, and preweaning calf performance. Journal of Animal Science, Champaign, v. 95, n. 12, p. 5597-5605, 2017. DOI: 10.2527/jas2017.1828
DUMMER, L. A.; CONCEIÇÃO, F. R.; NIZOLI, L. Q.; MORAES, C. M.; ROCHA, A. R.; SOUZA, L. L.; ROOS, T.; VIDOR, T.; LEITE, F. P. Cloning and expression of a truncated form of envelope glycoprotein D of Bovine herpesvirus type 5 in methylotrophic yeast Pichia pastoris. Journal of Virological Methods, Amsterdam, v. 161, n. 1, p. 84-90, 2009. DOI: 10.1016/j.jviromet.2009.05.022
HALL, J. A.; BOBE, G.; VORACHEK, W. R.; HUGEJILETU.; GORMAN, M. E.; MOSHER, W. D.; PIRELLI, G. J. Effects of feeding selenium-enriched alfalfa hay on immunity and health of weaned beef calves. Biological Trace Element Research, Clifton, v. 156, n. 1-3, p. 96-110, 2013. DOI: 10.1007/s12011-013-9843-0
HALL, J. A.; HARWELL, A. M.; VAN SAUN, R. J.; VORACHEK, W. R.; STEWART, W. C.; GALBRAITH, M. L.; HOOPER, K. J.; HUNTER, J. K.; MOSHER, W. D.; PIRELLI, G. J. Agronomic biofortification with selenium: effects on whole blood selenium and humoral immunity in beef cattle. Animal Feed Science and Technology, Amsterdam, v. 164, n. 3, p. 184-190, 2011. DOI: 10.1016/j.anifeedsci.2011.01.009
JAHANIAN, R.; KHALIFEH-GHOLI, M. Marginal dificiencies of dietary arginine and methionine could suppress growth performance and immunological responses in broiler chickens. Journal of Animal Physiology and Animal Nutrition, Berlin, v. 102, n. 1, p. e11-e20, 2018. DOI: 10.1111/jpn.12695
JIAO, X.; MA, W.; CHEN, Y.; LI, Z. Effects of amino acids supplementation in low crude protein diets on growth performance, carcass traits and serum parameters in finishing gilts. Animal Science Journal, Sendai v. 87, n. 10, p. 1252-1257, 2016. DOI: 10.1111/asj.12542
KUBINSKA, M.; TYKALOWSKI, B.; KONCICKI, A.; JANKOWSKI, J. Biochemical and immunological responses of young turkeys to vaccionation against Ornithobacterium rhinotracheale and different levels of dietary methionine. Polish Journal of Veterinary Sciences, Olsztyn, v. 18, n. 4, p. 807-816, 2015. DOI: 10.1515/pjvs-2015-0105
LOWMAN, B. G.; SCOTT, N.; SOMERVILLE, S. Condition scoring beef cattle. In: LOWMAN, B. G.; SCOTT, N.; SOMERVILLE, S. East of scotland college of agriculture. Edinburgh, EB: Edinburgh School of Agriculture. 1973.
MANDAL, G. P.; DASS, R. S.; ISORE, D. P.; GARG, A. K.; RAM, G. C. Effect of zinc supplementation from two sources on growth, nutrient utilization and immune response in male crossbred cattle (Bos indicus x Bos taurus) bulls. Animal Feed Science and Technology, Amsterdam, v. 138, n. 1, p. 1-12, 2007. DOI: 10.1016/j.anifeedsci.2006.09.014
MAYR, A.; BACHMANN, P. A.; BIBRACK, B. M.; WITHMANN, G. Virologische Arbeitsmethoden - Sicherheit bei virologischen arbeiten - Biomestrische Methoden. Stutgart, ST: Gustav Fischer Verlag, 1982.
McMENIMAN, N. P. Methods of estimating intake of grazing animal. REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 1997, Juiz de Fora, MG. Anais... Juiz de Fora, MG: Sociedade Brasileira de Zootecnia, 1997. p. 131.
NEMEC, L. M.; RICHARDS, J. D.; ATWELL, C. A.; DIAZ, D. E.; ZANTON, G. I.; GRESSLEY, T. F. Immune responses in lactating Holstein cows supplemented with Cu, Mn, and Zn as sulfates or methionine hydroxy analogue chelates. Journal Dairy Science, Champaign, v. 95, n. 8, p. 4568-4577, 2012. DOI: 10.3168/jds.2012-5404
OSORIO, J. S.; JI, P.; DRACKLEY, J. K.; LUCHINI, D.; LOOR, J. J. Supplemental Smartamine M or MetaSmart during the transition period benefits postpartal cow performance and blood neutrophil function. Journal Dairy Science, Champaign, v. 96, n. 10, p. 6248-6263, 2013. DOI: 10.3168/jds.2012-5790
PAUL, S. S.; DEY, A. Nutrition in health and immune function of ruminantes. Indian Journal of Animal Sciences, New Delhi, v. 2, n. 85, p. 103-112, 2015.
SANTOS, M. E. R.; FONSECA, D. M.; EUCLIDES, V. P. B.; RIBEIRO JUNIOR, J. I. R.; NASCIMENTO JUNIOR, D. N.; MOREIRA, L. M. Produção de bovinos em pastagens de capim-braquiária diferidas. Revista Brasileira de Zootecnia, Viçosa, MG, v. 38, n. 4, p. 635-642, 2009. DOI: 10.1590/S1516-35982009000400007
SIEDLER, B. S. Avaliação de uma vacina de aplicação intravaginal contra o Herpesvírus bovino tipo 5 (BoHV-5) associada a subunidade B recombinante da enterotoxina Termolábil de Escherichia coli (rLTB). 2012. Dissertação (Mestrado em Ciências) - Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas.
SODER, K. J.; HOLDEN, L. A. Lymphocyte proliferation response of lactating dairy cows fed varying concentrations of rumen-protected methionine. Journal Dairy Science, Champaign, v. 82, n. 9, p. 1935-1942, 1999. DOI: 10.3168/jds.S0022-0302(99)75429-9
SPEARS, J. W.; HARVEY, R. W.; BROWN, T. T. Effects os zinc methionine and zinc oxide on performance, blood characteristics, and antibody titer response to viral vaccination in stressed feeder calves. Journal of the American Veterinary Medical Association, Chicago, v. 199, n. 12, p. 1731-1733, 1991.
SPEARS, J. W.; KEGLEY, E. B. Effect of zinc source (zinc oxide vs zinc proteinate) and level on performance, carcass characteristics, and immune response of growing and finishing steers. Journal of Animal Science, Champaign, v. 80, n. 10, p. 2747-2752, 2002. DOI: 10.2527/2002.80102747x
SWECKER, W. S. Trace mineral feeding and assessment. The Veterinary Clinics of North America. Food Animal Practice, Philadelphia, v. 30, n. 3, p. 671-688, 2014. DOI: 10.1016/j.cvfa.2014.07.008
UNITED STATES DEPARTMENT OF AGRICULTURE - USDA, Animal and plant health inspection service. USDA, 2005. p. 683-684. Disponível em: https://www.govinfo.gov/content/pkg/CFR-2012-title9-vol1/pdf/CFR-2012-title9-vol1-sec113-216.pdf. Acesso em: 05 abr. 2019.
VAILATI-RIBONI, M.; ZHOU, Z.; JACOMETO, C. B.; MINUTI, A.; TREVISI, E.; LUCHINI, D. N.; LOOR, J. J. Supplementation with rumen-protected methionine or choline during the transition period influences whole-blood immune response in periparturient dairy cows. Journal of Dairy Science, Champaign, v. 100, n. 5, p. 3958-3968, 2017. DOI: 10.3168/jds.2016-11812
WATERMAN, R. C.; UJAZDOWSKI, V. L.; PETERSEN, M. K. Effects of rumen-protected methionine on plasma amino acid concentrations during a period of weight loss for late gestating beef heifers. Amino Acids, Wien, v. 43, n. 5, p. 2165-2177, 2012. DOI: 10.1007/s00726-012-1301-3
WEISS, W. P. Effects of feeding diets composed of corn silage and a corn milling product with and without supplemental lysine and methionine to dairy cows. Journal of Dairy Science, Champaign, v. 102, n. 3, p. 2075-2084, 2019. DOI: 10.3168/jds.2018-15535
ZANG, Y.; SAMII, S. S.; PHIPPS, Z. C.; TAGER, L. R.; McFADDEN, J. W.; KRAUSE, K. M. Comparative effects of multiple sources of rumen-protected methionine on milk production and serum amino acid levels in mid-lactation dairy cows. The Professional Animal Scientist, Michigan, v. 33, n. 6, p. 692-699, 2017. DOI: doi.org/10.15232/pas.2017-01641
ZHOU, Z.; BULGARI, O.; VAILATI-RIBONI, M.; TREVISI, E.; BALLOU, M. A.; LUCHINI, D. N.; LOOR, J. J. Rumen-protected methionine compared with rumen-protected choline improves immunometabolic status in dairy cows during the peripartal period. Journal of Dairy Science, Champaign, v. 99, n. 11, p. 8956-8969, 2016. DOI: 10.3168/jds.2016-10986
ZHOU, Z.; FERDOUS, F.; MONTAGNER, P.; LUCHINI, D. N.; CORRÊA, M. N.; LOOR, J. J. Methionine and choline supply during the peripartal period alter polymorphonuclear leukocyte immune response and immunometabolic gene expression in Holstein cows. Journal of Dairy Science, Champaign, v. 101, n. 11, p. 10374-10382, 2018. DOI: 10.3168/jds.2018-14972
ZHOU, Z.; VAILATI-RIBONI, M.; LUCHINI, D. N.; LOOR, J. J. Methionine and choline supply during the periparturient period alter plasma amino acid and one-carbon metabolism profiles to various extents: potential role in hepatic metabolism and antioxidant status. Nutrients, Basel, v. 9, n. 10, p. 1-19, 2017. DOI: 10.3390/nu9010010
CERONE, S. I.; SANSINANEA, A. S.; AUZA, N. J. Copper deficiency alters the immune response of bovine. Nutrition Research, New York, v. 15, n. 9, p. 1333-1341, 1995. DOI: 10.1016/0271-5317(95)02008-J
CLEMENTS, A. R.; IRELAND, F. A.; FREITAS, T.; TUCKER, H.; SHIKE, D. W. Effects of supplementing methionine hydroxy analog on beef cow performance, milk production, reproduction, and preweaning calf performance. Journal of Animal Science, Champaign, v. 95, n. 12, p. 5597-5605, 2017. DOI: 10.2527/jas2017.1828
DUMMER, L. A.; CONCEIÇÃO, F. R.; NIZOLI, L. Q.; MORAES, C. M.; ROCHA, A. R.; SOUZA, L. L.; ROOS, T.; VIDOR, T.; LEITE, F. P. Cloning and expression of a truncated form of envelope glycoprotein D of Bovine herpesvirus type 5 in methylotrophic yeast Pichia pastoris. Journal of Virological Methods, Amsterdam, v. 161, n. 1, p. 84-90, 2009. DOI: 10.1016/j.jviromet.2009.05.022
HALL, J. A.; BOBE, G.; VORACHEK, W. R.; HUGEJILETU.; GORMAN, M. E.; MOSHER, W. D.; PIRELLI, G. J. Effects of feeding selenium-enriched alfalfa hay on immunity and health of weaned beef calves. Biological Trace Element Research, Clifton, v. 156, n. 1-3, p. 96-110, 2013. DOI: 10.1007/s12011-013-9843-0
HALL, J. A.; HARWELL, A. M.; VAN SAUN, R. J.; VORACHEK, W. R.; STEWART, W. C.; GALBRAITH, M. L.; HOOPER, K. J.; HUNTER, J. K.; MOSHER, W. D.; PIRELLI, G. J. Agronomic biofortification with selenium: effects on whole blood selenium and humoral immunity in beef cattle. Animal Feed Science and Technology, Amsterdam, v. 164, n. 3, p. 184-190, 2011. DOI: 10.1016/j.anifeedsci.2011.01.009
JAHANIAN, R.; KHALIFEH-GHOLI, M. Marginal dificiencies of dietary arginine and methionine could suppress growth performance and immunological responses in broiler chickens. Journal of Animal Physiology and Animal Nutrition, Berlin, v. 102, n. 1, p. e11-e20, 2018. DOI: 10.1111/jpn.12695
JIAO, X.; MA, W.; CHEN, Y.; LI, Z. Effects of amino acids supplementation in low crude protein diets on growth performance, carcass traits and serum parameters in finishing gilts. Animal Science Journal, Sendai v. 87, n. 10, p. 1252-1257, 2016. DOI: 10.1111/asj.12542
KUBINSKA, M.; TYKALOWSKI, B.; KONCICKI, A.; JANKOWSKI, J. Biochemical and immunological responses of young turkeys to vaccionation against Ornithobacterium rhinotracheale and different levels of dietary methionine. Polish Journal of Veterinary Sciences, Olsztyn, v. 18, n. 4, p. 807-816, 2015. DOI: 10.1515/pjvs-2015-0105
LOWMAN, B. G.; SCOTT, N.; SOMERVILLE, S. Condition scoring beef cattle. In: LOWMAN, B. G.; SCOTT, N.; SOMERVILLE, S. East of scotland college of agriculture. Edinburgh, EB: Edinburgh School of Agriculture. 1973.
MANDAL, G. P.; DASS, R. S.; ISORE, D. P.; GARG, A. K.; RAM, G. C. Effect of zinc supplementation from two sources on growth, nutrient utilization and immune response in male crossbred cattle (Bos indicus x Bos taurus) bulls. Animal Feed Science and Technology, Amsterdam, v. 138, n. 1, p. 1-12, 2007. DOI: 10.1016/j.anifeedsci.2006.09.014
MAYR, A.; BACHMANN, P. A.; BIBRACK, B. M.; WITHMANN, G. Virologische Arbeitsmethoden - Sicherheit bei virologischen arbeiten - Biomestrische Methoden. Stutgart, ST: Gustav Fischer Verlag, 1982.
McMENIMAN, N. P. Methods of estimating intake of grazing animal. REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 1997, Juiz de Fora, MG. Anais... Juiz de Fora, MG: Sociedade Brasileira de Zootecnia, 1997. p. 131.
NEMEC, L. M.; RICHARDS, J. D.; ATWELL, C. A.; DIAZ, D. E.; ZANTON, G. I.; GRESSLEY, T. F. Immune responses in lactating Holstein cows supplemented with Cu, Mn, and Zn as sulfates or methionine hydroxy analogue chelates. Journal Dairy Science, Champaign, v. 95, n. 8, p. 4568-4577, 2012. DOI: 10.3168/jds.2012-5404
OSORIO, J. S.; JI, P.; DRACKLEY, J. K.; LUCHINI, D.; LOOR, J. J. Supplemental Smartamine M or MetaSmart during the transition period benefits postpartal cow performance and blood neutrophil function. Journal Dairy Science, Champaign, v. 96, n. 10, p. 6248-6263, 2013. DOI: 10.3168/jds.2012-5790
PAUL, S. S.; DEY, A. Nutrition in health and immune function of ruminantes. Indian Journal of Animal Sciences, New Delhi, v. 2, n. 85, p. 103-112, 2015.
SANTOS, M. E. R.; FONSECA, D. M.; EUCLIDES, V. P. B.; RIBEIRO JUNIOR, J. I. R.; NASCIMENTO JUNIOR, D. N.; MOREIRA, L. M. Produção de bovinos em pastagens de capim-braquiária diferidas. Revista Brasileira de Zootecnia, Viçosa, MG, v. 38, n. 4, p. 635-642, 2009. DOI: 10.1590/S1516-35982009000400007
SIEDLER, B. S. Avaliação de uma vacina de aplicação intravaginal contra o Herpesvírus bovino tipo 5 (BoHV-5) associada a subunidade B recombinante da enterotoxina Termolábil de Escherichia coli (rLTB). 2012. Dissertação (Mestrado em Ciências) - Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas.
SODER, K. J.; HOLDEN, L. A. Lymphocyte proliferation response of lactating dairy cows fed varying concentrations of rumen-protected methionine. Journal Dairy Science, Champaign, v. 82, n. 9, p. 1935-1942, 1999. DOI: 10.3168/jds.S0022-0302(99)75429-9
SPEARS, J. W.; HARVEY, R. W.; BROWN, T. T. Effects os zinc methionine and zinc oxide on performance, blood characteristics, and antibody titer response to viral vaccination in stressed feeder calves. Journal of the American Veterinary Medical Association, Chicago, v. 199, n. 12, p. 1731-1733, 1991.
SPEARS, J. W.; KEGLEY, E. B. Effect of zinc source (zinc oxide vs zinc proteinate) and level on performance, carcass characteristics, and immune response of growing and finishing steers. Journal of Animal Science, Champaign, v. 80, n. 10, p. 2747-2752, 2002. DOI: 10.2527/2002.80102747x
SWECKER, W. S. Trace mineral feeding and assessment. The Veterinary Clinics of North America. Food Animal Practice, Philadelphia, v. 30, n. 3, p. 671-688, 2014. DOI: 10.1016/j.cvfa.2014.07.008
UNITED STATES DEPARTMENT OF AGRICULTURE - USDA, Animal and plant health inspection service. USDA, 2005. p. 683-684. Disponível em: https://www.govinfo.gov/content/pkg/CFR-2012-title9-vol1/pdf/CFR-2012-title9-vol1-sec113-216.pdf. Acesso em: 05 abr. 2019.
VAILATI-RIBONI, M.; ZHOU, Z.; JACOMETO, C. B.; MINUTI, A.; TREVISI, E.; LUCHINI, D. N.; LOOR, J. J. Supplementation with rumen-protected methionine or choline during the transition period influences whole-blood immune response in periparturient dairy cows. Journal of Dairy Science, Champaign, v. 100, n. 5, p. 3958-3968, 2017. DOI: 10.3168/jds.2016-11812
WATERMAN, R. C.; UJAZDOWSKI, V. L.; PETERSEN, M. K. Effects of rumen-protected methionine on plasma amino acid concentrations during a period of weight loss for late gestating beef heifers. Amino Acids, Wien, v. 43, n. 5, p. 2165-2177, 2012. DOI: 10.1007/s00726-012-1301-3
WEISS, W. P. Effects of feeding diets composed of corn silage and a corn milling product with and without supplemental lysine and methionine to dairy cows. Journal of Dairy Science, Champaign, v. 102, n. 3, p. 2075-2084, 2019. DOI: 10.3168/jds.2018-15535
ZANG, Y.; SAMII, S. S.; PHIPPS, Z. C.; TAGER, L. R.; McFADDEN, J. W.; KRAUSE, K. M. Comparative effects of multiple sources of rumen-protected methionine on milk production and serum amino acid levels in mid-lactation dairy cows. The Professional Animal Scientist, Michigan, v. 33, n. 6, p. 692-699, 2017. DOI: doi.org/10.15232/pas.2017-01641
ZHOU, Z.; BULGARI, O.; VAILATI-RIBONI, M.; TREVISI, E.; BALLOU, M. A.; LUCHINI, D. N.; LOOR, J. J. Rumen-protected methionine compared with rumen-protected choline improves immunometabolic status in dairy cows during the peripartal period. Journal of Dairy Science, Champaign, v. 99, n. 11, p. 8956-8969, 2016. DOI: 10.3168/jds.2016-10986
ZHOU, Z.; FERDOUS, F.; MONTAGNER, P.; LUCHINI, D. N.; CORRÊA, M. N.; LOOR, J. J. Methionine and choline supply during the peripartal period alter polymorphonuclear leukocyte immune response and immunometabolic gene expression in Holstein cows. Journal of Dairy Science, Champaign, v. 101, n. 11, p. 10374-10382, 2018. DOI: 10.3168/jds.2018-14972
ZHOU, Z.; VAILATI-RIBONI, M.; LUCHINI, D. N.; LOOR, J. J. Methionine and choline supply during the periparturient period alter plasma amino acid and one-carbon metabolism profiles to various extents: potential role in hepatic metabolism and antioxidant status. Nutrients, Basel, v. 9, n. 10, p. 1-19, 2017. DOI: 10.3390/nu9010010
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2019-09-30
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Lopes, M. G., Dominguez, J. H. E., Peter, C. M., Santos, E., Rodrigues, P. A., Rodrigues, P. R. C., … Fischer, G. (2019). Humoral immune response in beef heifers supplemented with mineral salt with or without the addition of rumen-protected methionine. Semina: Ciências Agrárias, 40(6Supl2), 3057–3068. https://doi.org/10.5433/1679-0359.2019v40n6Supl2p3057
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