Análise de proteínas de leucócitos polimorfonucleares de gatos domésticos
DOI:
https://doi.org/10.5433/1679-0359.2024v45n6p1873Palavras-chave:
Felis catus, Grânulos, Neutrófilos, Proteômica.Resumo
Os neutrófilos são a primeira linha de defesa contra os microrganismos, sendo assim, uma célula muito importante na imunidade inata. Objetivou-se com este estudo caracterizar o proteoma de neutrófilo de Felis catus. Para isso, os granulócitos foram obtidos de sangue de gatos, purificados e utilizados 1x107 de células para identificação das proteínas.Foram identificadas 102 proteínas de neutrófilos de gatos, sendo classificadas conforme a abundância. A proteoglicano-3 associada a alguns processos biológicos como ativação dos neutrófilos e resposta imune, foi a proteína mais abundante. Dentre os processos, nas rotas metabólicas a maior quantidade de proteínas identificadas está envolvida na glicólise, que foram nove proteínas. Verificou-se também as proteínas envolvidas em função molecular, no componente celular e nos processos biológicos. Espera-se que a identificação das proteínas nesse estudo auxilie as futuras pesquisas elucidando algumas particularidades da espécie contribuindo com possíveis marcadores ou fármacos auxiliando no diagnóstico rápido e não invasivo de doenças que acometem em particular os felinos.
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