Pathogenicity and virulence of entomopathogenic nematodes against Dichelops melacanthus Dallas (Hemiptera: Pentatomidae)
DOI:
https://doi.org/10.5433/1679-0359.2019v40n4p1417Keywords:
Microbial control, Steinernema, Heterorhabditis, Belly-green stink bug.Abstract
Infestations of Dichelops melacanthus (Dallas; Hemiptera: Pentatomidae) in corn and wheat in Brazil, and the subsequent damage, have increased in recent years, mainly owing to this insect’s ability to survive the off-season. The control of this insect is mainly carried out with chemical insecticides, but the development of alternative methods, such as biological control, can contribute to a more sustainable management. Thus, the objective of this study was to evaluate the potential of entomopathogenic nematodes (EPNs) for the control of D. melacanthus. A selection test was performed with 15 isolates of genera Steinernema and Heterorhabditis regarding their pathogenicity and virulence on adults of D. melacanthus. Concentration (10, 20, 40, 50, and 100 infective juveniles (IJs)/cm2) and greenhouse tests were carried out only with the Steinernema feltiae isolate (IBCB-n 47). All experiments were conducted in a completely randomized design. The selection test data were submitted to the Scott-Knott averages test (P ? 0.05), and those from the greenhouse test to the Student's t-test. The results of the concentration assay were subjected to regression analysis. All isolates showed pathogenicity and virulence in adults of D. melacanthus. The isolates GL (Heterorhabditis amazonensis), IBCB-n27 (Steinernema sp.), and RSC05 (H. amazonensis) were the most virulent (80.0, 82.0, and 88.0% mortality, respectively). The higher concentrations of S. feltiae (50 and 100 IJs/cm²) were responsible for the highest mortality rates of green belly stink bug (88.0 and 86.0%, respectively). In the greenhouse test, S. feltiae caused higher mortality (38%) than the control.Downloads
References
ALVES, V. S.; MOINO JUNIOR, A.; SANTA-CECILIA, L. V. C.; ANDALÓ, V.; SOUZA, G. C. Patogenicidade de nematoides entomopatogênicos a cochonilha-da-raiz-do-cafeeiro Dysmicoccus texensis (Tinsley) (Hemiptera: Pseudococcidae) em laboratório. Arquivos do Instituto Biológico, São Paulo, v. 76, n. 1, p. 67-73, 2009a.
ÁVILA, C. J.; DUARTE, M. M. Eficiência de inseticidas, aplicados nas sementes e em pulverização, no controle do percevejo barriga-verde Dichelops melacanthus (Dallas) (Hemiptera: Pentatomidae), na cultura do milho. BioAssay, Piracicaba, v. 7, n. 6, p. 1-6, 2012. DOI: 10.14295/BA.v7.0.72
BATISTA, E. S. D. P.; AUAD, A. M.; ANDALÓ, V.; MONTEIRO, C. M. D. O. Virulence of entomopathogenic nematodes (Rhabditida: Steinernematidae, Heterorhabditidae) to spittlebug Mahanarva spectabilis (Hemiptera: Cercopidae). Arquivos do Instituto Biológico, São Paulo, v. 81, n. 2, p. 145-149, 2014. DOI: 10.1590/1808-1657001152012
BEDDING, R. A.; AKHURST, R. J. A. Simple technique for the detection of insect parasitic rhabditid nematodes in soil. Nematologica, Leiden, v. 21, n. 1, p. 109-110, 1975. DOI: 10.1163/187529275X00419
BRUSTOLIN, C.; BIANCO, R.; NEVES, P. M. O. J. Inseticidas em pré e pós-emergência do milho (Zea mays L.), associados ao tratamento de sementes, sobre Dichelops melacanthus (Dallas) (Hemiptera: Pentatomidae). Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 10, n. 3, p. 215-223, 2011. DOI: 10.18512/1980-6477/rbms.v10n3p215-223
CRUZ, I.; BIANCO, R.; REDOAM, A. Potential risk of losses in maize caused by Dichelops melacanthus (Dallas) (Hemiptera: Pentatomidae) in Brazil. Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 15, n. 3, p. 386-397, 2016. DOI: 10.18512/1980-6477/rbms.v15n3p386-397
DEL VALLE, E. E.; DOLINSKI, C.; SOUZA, R. M. Dispersal of Heterorhabditis baujardi LPP7 (Nematoda: Rhabditida) applied to the soil as infected host cadavers. International Journal Pest Management, Londres, v. 54, n. 2, p. 115-122, 2008. DOI: 10.1080/09670870701660579
FUGA, C. A. G.; FERNANDES, R. H.; LOPES, E. A. Nematoides entomopatogênicos. Revista Trópica: Ciências Agrárias e Biológicas, Chapadinha, v. 6, n. 3, p. 56-75, 2012.
GAUGLER, R.; WANG, Y.; CAMPBELL, J. F. Aggressive and evasive behaviors in Popillia japonica (Coleoptera: Scarabaeidae) larvae: defences against entomopathogenic nematode attack. Journal Invertebrate Pathology, New York, v. 64, n. 3, p. 193-199, 1994. DOI: 10.1016/S0022-2011(94)90150-3
GREWAL, O. S.; NARDO, E. A. B.; AGUILLERA, M. M. Entomopathogenic nematodes: potential for exploration and use in South America. Neotropical Entomology, Londrina, v. 30, n. 2, p. 191-205, 2001. DOI: 10.1590/S1519-566X2001000200001
LAUMANN, R. A.; MORAES, M. C. B.; SILVA, J. P.; VIEIRA, A. M. C.; SILVEIRA, S.; BORGES, M. I. Egg parasitoid wasps as natural enemies of the neotropical stink bug Dichelops melacanthus. Pesquisa Agropecuária Brasileira, Brasília, v. 45, n. 5, p. 442-449, 2010. DOI: 10.1590/S0100-204X2010000500002
MARTINS, G. L. M.; MARUYAMA, L. C. T.; TOMQUELSKI, G. V.; MARUYAM, L. C. T. Efeito de alguns inseticidas sobre Spodoptera frugiperda (Lepidoptera: Noctuidae) e Dichelops sp. (Homoptera: Pentatomidae) na fase inicial da cultura do milho. Revista Cientifica Eletrônica da Agronomia, Garça, v. 5, n. 9, p. 1-11, 2006.
MELO, E. L. M.; ORTEGA-OJEDA, C. A.; GAIGL, A.; EHLERS, R. U.; BELLOTTI, A. C. Evaluation of two commercial strains of entomonematodes as control agents of Cyrtomenus bergi Froeschner (Hemiptera: Cydnidae). Revista Colombiana de Entomologia, Bogotá, v. 32, n. 1, p. 31-38, 2006.
MOLINA, J. P. A.; LÓPEZ, N. J. C. Producción in vivo de três entomonematodos con dos sistemas de infección en dos hospedantes. Revista Colombiana de Entomologia, Bogotá, v. 27, n. 1-2, p. 73-78, 2001.
ROZA-GOMES, M. F.; SALVADORI, J. R.; PEREIRA, P. R. V. S.; PANIZZI, A. R. Injúrias de quatro espécies de percevejos pentatomídeos em plântulas de milho. Ciência Rural, Santa Maria, v. 41, n. 7, p. 1115-1119, 2011.
SALGUERO, C. A. M.; PARDEY, A. E. B.; NÚÑEZ, J. C. L.; VALDERRAMA, U. C.; SÁNCHEZ, G. D. R. S. Virulence of entomopathogenic nematodes to control Aeneolamia varia (Hemiptera: Cercopidae) in sugarcane. Revista Colombiana de Entomologia, Bogotá, v. 38, n. 2, p. 260-265, 2012.
SELVAN, S.; CAMPBELL, J. F.; GAUGLER, R. Density-dependent effects on entomopathogenic nematodes (Heterorhabditidae and Steinernematidae) within an insect host. Journal Invertebrate Pathology, New York, v. 62, n. 3, p. 278-284, 1993. DOI: 10.1006/jipa.1993.1113
SILVA, J. J.; VENTURA, M. U.; SILVA, F. A. C.; PANIZZI, A. R. Population dynamics of Dichelops melacanthus (Dallas) (Heteroptera: Pentatomidae) on host plants. Neotropical Entomology, Londrina, v. 42, n. 2, p. 141-145, 2013. DOI: 10.1007/s13744-012-0104-2
SMANIOTTO, L. F.; PANIZZI, A. R. Interactions of selected species of stink bugs (Hemiptera: Heteroptera: Pentatomidae) from leguminous crops with plants in the Neotropics. Florida Entomologist, Lutz, v. 98, n. 1, p. 7-17, 2015. DOI: 10.1653/024.098.0103
WHITE, G. F. A method for obtaining infective nematodes larvae from cultures. Science, Washington DC, v. 66, n. 1709, p. 302-303, 1927.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2019 Semina: Ciências Agrárias
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Semina: Ciências Agrárias adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.