In vitro organogenesis of potato (Solanum tuberosum L.) cultivar Atlantic for the genetic transformation
DOI:
https://doi.org/10.5433/1679-0359.2013v34n3p1055Keywords:
Micropropagation, Tissue culture, Agrobacterium tumefaciens.Abstract
The obtaining of commercial cultivars of potato with resistance to the phytopatogen is a very promising alternative that the genetic engineering can provide, through the introduction of exogenous genes for the genetic transformation. In order to establish an efficient system of transformation, it is crucial first to optimize in vitro organogenesis protocol. This work aimed to establish an efficient protocol for the genetic transformation of potato cv. Atlantic. The efficiency of the in vitro organogenesis is influenced by the explant type and by the kind and concentrations of growth regulators used. Shoot segments of potato cv. Atlantic present better organogenesis capacity than leaf explants. It is recommended to use the woody plant medium (WPM) supplied with 1,0 mg L-1 of naphthalene acetic acid (NAA) + 5,0 mg L-1 of zeatin riboside (ZEA), to obtain shoots from shoot segments, and the carbenicilin (Cb) added to this medium increased this formation. For the process to enlarge shoots, this same antibiotic when added to the WPM medium in growing concentrations (100; 250; 500 mg L-1) promoted a decrease in the height of plants, number of shoots and length of roots. After the co-cultivation of shoots segments with Agrobacterium tumefaciens these concentrations of Cb are not efficient in the elimination of the bacteria, which commits the organogenesis. However, it is possible to establish a protocol of in vitro organogenesis of potato cv. Atlantic starting from shoots segments not co-cultivated. These results will be useful for futures experiments of genetic transformation with this and another commercials cultivars of potato.
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