Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters

Authors

  • Fernanda Schoma de Oliveira Universidade Estadual do Centro-Oeste
  • Margarete Kimie Falbo Universidade Estadual do Centro-Oeste
  • Itacir Eloi Sandini Universidade Estadual do Centro-Oeste
  • Larissa Emy Ishiy Universidade Estadual do Centro-Oeste

DOI:

https://doi.org/10.5433/1679-0359.2011v32n2p717

Keywords:

Serum stability, Sheeps, Time of storage.

Abstract

This study aimed to evaluate the effect of freezing (- 20 ºC) and time of storage of serum in the determination of biochemical values in sheep. It was held at the Universidade Estadual do Centro-Oeste in Guarapuava – PR. The serum was obtained from 32 Corriedale x Ille de France crossbred lambs, four months old. Nine hundred and sixty tests were performed, the first immediately after sampling (time zero), after 7, 14, 21, 28 days after blood collection. The analytes tested were aspartate aminotranferase (AST), urea, total protein, cholesterol, phosphorus and magnesium. The results showed that the time of storage of frozen samples (-20 ºC) was able to alter results of total protein, phosphorus, and magnesium. The serum measurements of AST, cholesterol and urea did not differ in different storage times.

Author Biographies

Fernanda Schoma de Oliveira, Universidade Estadual do Centro-Oeste

Médica Veterinária, Programa Universidade Sem Fronteiras, SETI, PR. Rua Silvas do Brasil, 450. Vila Beraldo. Prudentópolis, PR. 84400-000.

Margarete Kimie Falbo, Universidade Estadual do Centro-Oeste

Profª. da Universidade Estadual do Centro Oeste, Departamento Medicina Veterinária, UNICENTRO.

Itacir Eloi Sandini, Universidade Estadual do Centro-Oeste

Prof. da Universidade Estadual do Centro Oeste, Departamento Agronomia, UNICENTRO.

Larissa Emy Ishiy, Universidade Estadual do Centro-Oeste

Acadêmica do Curso de Medicina Veterinária da Universidade Estadual do Centro Oeste.

Published

2011-07-12

How to Cite

Oliveira, F. S. de, Falbo, M. K., Sandini, I. E., & Ishiy, L. E. (2011). Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters. Semina: Ciências Agrárias, 32(2), 717–722. https://doi.org/10.5433/1679-0359.2011v32n2p717

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