Equine semen cooled with chemically defined extender containing soy lecithin
DOI:
https://doi.org/10.5433/1679-0359.2022v43n6p2743Keywords:
Cryopreservation, Sperm, Soy Phosphatidylcholine, Skimmed milk, Stallion.Abstract
Equine semen has historically been chilled using milk-based media. However, the use of animal-based components presents several potential concerns, such as variability in formulations, microbial contamination and regulatory issues. We aimed to evaluate the potential of including different concentrations of soy lecithin (LS) in chemically defined Biggers, Whitten and Whittingham (BWW) medium for cooling equine semen to 15°C. Ejaculates were diluted as six different experimental groups: 1) BotuSêmen® (control); 2) BWW; 3) BWW + 1% LS; 4) BWW + 2% LS; 5) BWW + 4% LS and 6) BWW + 6% LS. BWW medium, did not preserve motility, velocity, straightness (STR), linearity (LIN), amplitude of lateral sperm head displacement (ALH), cross flagellar beat frequency (BCF), functional and structural integrity of equine spermatozoa during 24 h of refrigeration when compared to BotuSêmen® (P <0.05). The use of BWW for cooling equine semen was only possible with the addition of LS, being the concentrations equal or higher than 2% better, because they preserved total motility, curvilinear velocity (VCL) and LIN with the same potential of BotuSêmen® (P >0.05). Nevertheless, BotuSêmen® showed superiority in preserving the percentage of sperm progressive motility, average path velocity (VAP), linear progressive velocity (VSL) and BCF during cooling compared to the other extenders (P <0.05). The inclusion of soy lecithin, from 2 to 6% in the BWW medium, allowed maintaining the viability of equine semen cooled at 15ºC for up to 24 hours.
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References
Akhter, S., Ansari, M. S., Rakha, B. A., Ullah, N., Andrabi, S. M., & Khalid, M. (2011). In vitro evaluation of liquid stored buffalo semen at 5c diluted in soya lecithin-based extender (Bioxcell), tris-citric egg yolk, skim-milk and egg yolk-citrate extenders. Reproduction in Domestic Animals, 46(1), 45-49. doi: 10.1111/j.1439-0531.2009.01561.x DOI: https://doi.org/10.1111/j.1439-0531.2009.01561.x
Alamaary, M., Haron, A. W., Ali, M., Hiew, M., Adamu, L., & Peter, I. (2019). Effects of four extenders on the quality of frozen semen in Arabian stallions. Veterinary World, 12(1), 34-40. doi: 10.14202/vetworld.2019.34-40 DOI: https://doi.org/10.14202/vetworld.2019.34-40
Ashaolu, T. J. (2020). Applications of soy protein hydrolysates in the emerging functional foods: a review. International Journal of Food Science & Technology, 55(2), 421-428. doi: 10.1111/ijfs.14380 DOI: https://doi.org/10.1111/ijfs.14380
Aurich, C., Seeber, P., & Müller-Schlösser, F. (2007). Comparison of different extenders with defined protein composition for storage of stallion spermatozoa at 5 degrees C. Reproduction in Domestic Animals, 42(4), 445-448. doi: 10.1111/j.1439-0531.2006.00828.x DOI: https://doi.org/10.1111/j.1439-0531.2006.00828.x
Biggers, J. D., Whitten, W. K., & Whittingham, D. G. (1971). The culture of mouse embryos in vitro. In J. C. Daniels (Ed.), Methods in mammalian embryology (pp. 86-116). San Francisco, CA: Freeman.
Bousseau, S., Brillard, J. P., Marquan-Le Guienne, B., Guerin, B., Camus, A., & Lechat, M. (1998). Comparison of bacteriological qualities of various egg yolk sources and the in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or lecithin-based diluents. Theriogenology, 50(5), 699-706. doi: 10.1016/s0093-691x(98)00175-7 DOI: https://doi.org/10.1016/S0093-691X(98)00175-7
Brasileiro, L. S., Segabinazzi, L. G. T. M., Menezes, E., Salgueiro, C. C., Novello, G., Scheeren, V. F. D. C., Alvarenga, M. A., & Nunes, J. F. (2019). Coconut water as an extender component for cooled equine sperm. Journal of Equine Veterinary Science, 78(1), 69-73. doi: 10.1016/j.jevs.2019.03.213 DOI: https://doi.org/10.1016/j.jevs.2019.03.213
Caldevilla, M., Ferrante, A., & Neild, D. (2020). Utilización de un medio con lecitina de soja para congelar semen equino. InVet, 22(1), 41-49.
Campos, G. A., Garcia, V. F. C., Freitas-Dell’Aqua, C. P., Segabinazzi, L. G. T. M., Maciel, L. F. S., Alvarenga, M. A., Papa, F. O., & Dell’Aqua, J. A. (2020). Sodium caseinate and cholesterol improve bad cooler stallion fertility. Journal of Equine Veterinary Science, 93(1), 103201. doi: 10.1016/j.jevs.2020.103201 DOI: https://doi.org/10.1016/j.jevs.2020.103201
Brazilian College of Animal Reproduction (2013). Manual de exame andrológico e avaliação de sêmen animal (3a ed.). BCAR.
Dalmazzo, A., Angrimani, D. S. R., Losano, J. D. A., Rocha, C. C., Sobrinho, C. A. B., Gurgel, J. R. C., Pacheco, P. I. M., Minazaki, C. K., Crusco, S. E., Nichi, M., & Barnabe, V. H. (2019). Insights into soy lecithin and egg yolk-based extenders for chilling canine spermatozoa. Zygote, 27(1), 17-24. doi: 10.1017/S0967199418000576 DOI: https://doi.org/10.1017/S0967199418000576
De Paz, P., Esteso, M. C., Alvarez, M., Mata, M., Chamorro, C. A., & Anel, L. (2010). Development of extender based on soybean lecithin for its application in liquid ram semen. Theriogenology, 74(4), 663-671. doi: 10.1016/j.theriogenology.2010.03.022 DOI: https://doi.org/10.1016/j.theriogenology.2010.03.022
Deng, L. (2021). Current progress in the utilization of soy-based emulsifiers in food applications-a review. Foods, 10(6), 1354. doi: 10.3390/foods10061354 DOI: https://doi.org/10.3390/foods10061354
Florez-Rodriguez, S. A., Arruda, R. P., Alves, M., Affonso, F. J., Carvalho, H. F., Lemes, K. M., Lançoni, R., Andrade, A. F., & Celeghini, E. (2014). Morpho functional characterization of cooled sperm with different extenders to use in equine-assisted reproduction. Journal of Equine Veterinary Science, 34(7), 911-917. doi: 10.1016/j.jevs.2014.04.007 DOI: https://doi.org/10.1016/j.jevs.2014.04.007
Forouzanfar, M., Sharafi, M., Hosseini, S. M., Ostadhosseini, S., Hajian, M., Hosseini, L., Abedi, P., Nili, N., Rahmani, H. R., & Nasr-Esfahani, M. H. (2010). In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology, 73(4), 480-487. doi: 10.1016/j.theriogenology.2009.10.005 DOI: https://doi.org/10.1016/j.theriogenology.2009.10.005
Gibb, Z., Lambourne, S. R., Quadrelli, J., Smith, N. D., & Aitken, R. J. (2015). L-carnitine and pyruvate are prosurvival factors during the storage of stallion spermatozoa at room temperature. Biology of Reproduction, 93(4), 104. doi: 10.1095/biolreprod.115.131326 DOI: https://doi.org/10.1095/biolreprod.115.131326
Harrison, R. A., & Vickers, S. E. (1990). Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. Journal of Reproduction and Fertility, 88(1), 343-352. doi: 10.1530/jrf.0.0880343 DOI: https://doi.org/10.1530/jrf.0.0880343
John, J. V., Park, H., Lee, H. R., Suh, H., & Kim, I. (2015). Simultaneous extraction of phosphatidylcholine and phosphatidylethanolamine from soybean lecithin. European Journal of Lipid Science and Technology, 117(10), 1647-1654. doi: 10.1002/ejlt.201400396 DOI: https://doi.org/10.1002/ejlt.201400396
Judde, A., Villeneuve, P., Rossignol-Castera, A., & Guillou, A. L. (2003). Antioxidant effect of soy lecithins on vegetable oil stability and their synergism with tocopherols. Journal of the American Oil Chemists' Society, 80(12), 1209-1215. doi: 10.1007/s11746-003-0844-4 DOI: https://doi.org/10.1007/s11746-003-0844-4
Melo, M. I. V., & Henry, M. (1999). Teste hiposmótico na avaliação de sêmen equino. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 51(1), 71-78. DOI: https://doi.org/10.1590/S0102-09351999000600009
Mousavi, S. M., Towhidi, A., Zhandi, M., Amoabedin, G., Mohammadi-Sangcheshmeh, A., Sharafi, M., & Hussaini, S. M. H. (2019). Comparison of two different antioxidants in a nano lecithin-based extender for bull sperm cryopreservation. Animal Reproduction Science, 209(1), 106171. doi: 10.1016/j.anireprosci.2019.106171 DOI: https://doi.org/10.1016/j.anireprosci.2019.106171
Mozafari, M. R. (2010). Nanoliposomes: preparation and analysis. Methods in Molecular Biology, 605(1), 29-50. doi: 10.1007 / 978-1-60327-360-2_2 DOI: https://doi.org/10.1007/978-1-60327-360-2_2
Murphy, C., English, A. M., Holden, A. S., & Fair, S. (2014). Cholesterol-loaded-cyclodextrins improve the post-thaw quality of stallion sperm. Animal Reproduction Science, 145(3-4), 123-129. doi: 10.1016/j.anireprosci.2014.01.013 DOI: https://doi.org/10.1016/j.anireprosci.2014.01.013
Nadri, T., Towhidi, A., Zeinoaldini, S., Martínez-Pastor, F., Mousavi, M., Noei, R., Tar, M., & Sangcheshmeh, A. M. (2019). Lecithin nanoparticles enhance the cryosurvival of caprine sperm. Theriogenology, 133(1), 38-44. doi: 10.1016/j.theriogenology.2019.04.024 DOI: https://doi.org/10.1016/j.theriogenology.2019.04.024
Naves, C. S., Beletti, M. E., Duarte, M. B., & Vieira, R. C. (2004). Avaliação da cromatina espermática em equinos com azul de toluidina e acridine orange. Bioscience Journal, 20(3), 117-124.
Nguyen, V. V., Ponchunchoovong, S., Kupittayanant, S., & Kupittayanant, P. (2019). Effects of egg yolk and soybean lecithin on sperm quality determined by computer-assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm. Veterinary Medicine and Science, 5(3), 345-360. doi: 10.1002/vms3.158 DOI: https://doi.org/10.1002/vms3.158
Nouri, H., Towwhidi, A., Zhandi, M., & Sadeghi, R. (2013). The effects of centrifuged egg yolk used with INRA plues soybean lecithin extender on semen quality to freeze Caspian horse semen. Journal of Equine Veterinary Science, 33(12), 1050-1053. doi: 10.1016/j.jevs.2013.03.184 DOI: https://doi.org/10.1016/j.jevs.2013.03.184
Novello, G., Podico, G., Segabinazzi, L. G. T. M., Lima, S. F., & Canisso, I. F. (2020). Stallion semen cooling using native phosphocaseinate-based extender and sodium caseinate cholesterol-loaded cyclodextrin-based extender. Journal of Equine Veterinary Science, 92(1), 103104 doi: 10.1016/j.jevs.2020.103104 DOI: https://doi.org/10.1016/j.jevs.2020.103104
Oliveira, G. C., Oliveira, B. M. M., Celeghini, E. C. C., Fernandes, C. B., & Mattos, C. B. (2013). Criopreservação do sêmen equino: uma revisão. Revista Brasileira de Reprodução Animal, 37(1), 23-28.
Papa, F. O., Felício, G. B., Melo-Oña, C. M., Alvarenga, M. A., De Vita, B., Trinque, C., Puoli, J. N., Fº., & Dell'Aqua, J. A., Jr. (2011). Replacing egg yolk with soybean lecithin in the cryopreservation of stallion semen. Animal Reproduction Science, 129(1-2), 73-77. doi: 10.1016/j.anireprosci.2011.10.006 DOI: https://doi.org/10.1016/j.anireprosci.2011.10.006
Plante, G., Lusignan, M. F., Lafleur, M., & Manjunath, P. (2015). Interaction of milk proteins and Binder of Sperm (BSP) proteins from boar, stallion and ram semen. Reproductive Biology and Endocrinology, 13(1), 92-112. doi: 10.1186/s12958-015-0093-1 DOI: https://doi.org/10.1186/s12958-015-0093-1
Ricker, J. V., Linfor, J. J., Delfino, W. J., Kysar, P., Scholtz, E. L., Tablin, F., Crowe, J. H., Ball, B. A., & Meyers, S. A. (2006). Equine sperm membrane phase behavior: the effects of lipid-based cryoprotectants. Biology of Reproduction, 74(2), 359-365. doi: 10.1095/biolreprod.105.046185 DOI: https://doi.org/10.1095/biolreprod.105.046185
Sorrenti, G., Bagnoli, A., Miraglia, V., Crocetta, F., Vitiello, V., Ristoratore, F., Cirino, P., Sansone, G., & Sordino, P. (2014). Investigating sperm cryopreservation in a model tunicate, Ciona intestinalis sp. A. Cryobiology, 68(1), 43-49. doi: 10.1016/j.cryobiol.2013.11.005 DOI: https://doi.org/10.1016/j.cryobiol.2013.11.005
Sun, L., He, M., Wu, C., Zhang, S., Dai, J., & Zhang, D. (2021). Beneficial influence of soybean lecithin nanoparticles on rooster frozen-thawed semen quality and fertility. Animals, 1(6), 1-12. doi: 10.3390/ani11061769 DOI: https://doi.org/10.3390/ani11061769
Swegen, A., Lambourne, S. R., Aitken, R. J., & Gibb, Z. (2016). Rosiglitazone improves stallion sperm motility, ATP content, and mitochondrial function. Biology of Reproduction, 95(5), 107, 1-12. doi: 10.1095/biolreprod.116.142687 DOI: https://doi.org/10.1095/biolreprod.116.142687
Vidament, M., Dupere, A. M., Julienne, P., Evain, A., Noue, P., & Palmer, E. (1997). Equine frozen semen: freezability and fertility field results. Theriogenology, 48(6), 907-917. doi: 10.1016/s0093-691x(97)00319 DOI: https://doi.org/10.1016/S0093-691X(97)00319-1
Wang, G., & Wang, T. (2008). Oxidative stability of egg and soy lecithin as affected by transition metal ions and pH in emulsion. Journal of Agricultural and Food Chemistry, 56(23), 11424-11431. doi: 10.1021/jf8022832 DOI: https://doi.org/10.1021/jf8022832
Watson, P. F., & Morris, G. J. (1987). Cold shock injury in animal cells. Symposia of the Society for Experimental Biology, 41(1), 311-340.
Zhao, J. Q., Xiao, G. L., Zhu, W. L., Fang, D., Li, N., Han, C. M., & Gao, Q. H. (2021). Ram semen preserved at 0 °C with soybean lecithin Tris-based extender substituted for egg yolk. Animal Bioscience, 34(2), 192-197. doi: 10.5713/ajas.20.0118 DOI: https://doi.org/10.5713/ajas.20.0118
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