Risk factors associated with the occurrence of multiresistant Staphylococcus spp. isolated from bovine subclinical mastitis in northern Brazil
DOI:
https://doi.org/10.5433/1679-0359.2022v43n2p901Keywords:
Risk factors, Mastitis, Multidrug resistance, Staphylococcus.Abstract
Staphylococcus bacteria are often associated with subclinical bovine mastitis. This study aimed to identify multiresistant Staphylococcus spp. associated with subclinical mastitis and the associated risk factors. Twenty-three dairy farms with a history of decrease in milk production, located in the lower Acre region, Brazil, were selected. An epidemiological questionnaire was provided in all farms. All animals were examined using the California Mastitis Test (CMT) and their milk samples were collected for bacterial culture. After isolation and identification, the disk diffusion antimicrobial susceptibility test was performed against nine classes of antimicrobials. Of the 339 cows examined using the CMT, 108 had mastitis. A total of 229 milk samples were collected from individual teats. MALDI-TOF MS found isolates belonging to eight species of Staphylococcus, in 101 of these samples. S. chromogenes (58.4%) demonstrated strongest resistance to the nine classes of antimicrobial active principles. Nineteen isolates with multidrug resistance phenotypic profile were identified. This phenotypic expression indicates wide circulation of resistant genes in this species. The presence of multidrug resistance in Staphylococcus spp. in this study was correlated with lack of water for cleaning the corral, which is a preventive factor, minimizing the transmission and persistence of pathogens in the farms.Downloads
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References
Aslanta, O., & Demir, C. (2016). Investigation of the antibiotic resistance and biofilm-forming ability of Staphylococcus aureus from subclinical bovine mastitis cases. Journal of Dairy Science, 99(11), 8607-8613. doi: 10.3168/jds.2016-11310
Casanova, V. P., Appio, J., Kohl, E., Michaelsen, T. R., Paim, S. D., Brunetto, T. R. Girardini, L. (2016). Bovine mastitis: prevalence and antimicrobial susceptibility profile and detection of genes associated with biofilm formation in Staphylococcus aureus. Semina: Ciências Agrárias, 37(3), 1369-1378. doi: 10.5433/1679-0359.2016v37n3p1369
Clinical & Laboratory Standards Institute (2018). Performance standards for antimicrobial disk susceptibility tests. Wayne: West Valley Road.
Freitas, C. H., Mendes, J. F., Villarreala, P. V., Santos, P. R., Gonçalves, C. L., Gindonis, V., Rantala, M. (2018). Occurrence and characterization of methicillin-resistant staphylococci from bovine mastitis milk samples in Finland. Acta Veterinaria Scandinavica, 55(1), 61. doi: 10.1186/1751-0147-55-61
Ganda, E. K., Bisinotto, R. S., Decter, D. H., & Bicalho, R. C. (2016). Evaluation of an on-farm culture system (accumast) for fast identification of milk pathogens associated with clinical mastitis in dairy cows. PLoS One, 11(5), 1-16. doi: 10.1371/journal.pone.0155314.
Israel, L. F. S., Rabello, R. F., Domingos, S. C. B., & Medeiros, L. S. (2018). Biofilm production by Staphylococcus chromogenes isolated from milk samples from bovine with mastitis. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 70(6), 1943-1949. doi: 10.1590/1678-4162-9866
Mello, P. L., Pinheiro, L., Martins, L. de A., Brito, M. A. V. P., Cunha, R. de S., & Cunha, M. de L. R. de S. (2017). Short communication: B Lactam resistance and vancomycin heteroresistance in Staphylococcus spp. isolated from bovine subclinical mastitis. Journal of Dairy Science, 100(8), 6567-6571. doi: 10.3168/jds.2016-12329
Mesquita, A. A. (2017). Impacto da mastite bovina por Sthapylococcus aureus e Streptococcus agalactiae. Tese de mestrado, Universidade Federal de Lavras. Lavras, MG, Brasil. Recuperado de http://repositorio.ufla.br/jspui/bitstream/1/28358/2/TESE_Impacto%20da%20Mastite%20bovina%20por%20Sthapylococcus%20aureus%20e%20Streptococcus%20agalactiae.pdf
Moritz, F., & Moritz, C. M. F. (2016). Resistência aos antimicrobianos em Staphylococcus spp. associados à mastite bovina. Revista de Ciência Veterinária e Saúde Pública, 3(2), 132-136. doi: 10.4025/revcivet.v3i2.34435
National Mastitis Council (2004). Procedures for collecting milk samples. Recuperado de https://www.nmconline.org/nmc-protocols-guidelines-and-procedures/.
Oliveira, C. S. F. (2015). Análise epidemiológica e bioeconômica da mastite bovina em rebanhos brasileiros. Tese de doutorado, Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil. Recuperado de https://repositorio.ufmg.br/bitstream/1843/BUBD-A5GMBM/1/texto_tese_final___camila_stefanie_ fonseca_de_oliveira.pdf
Papadopoulos, P., Papadopoulos, T., Angelidis, A. S., Boukouvala, E., Zdragas, A., Papa, A. Sergelidis, D. (2018). Prevalence of Staphylococcus aureus and of methicillin-resistant S. aureus (MRSA) along the production chain of dairy products in north-western Greece. Food Microbiology, 69,43-50. doi: 10.1016/j.fm.2017.07.016
Queiroz, A. M., & Souza, L. G. S. (2021). Análise da produção de leite de vaca no Estado do Acre. Scientia Naturalis, 3(1), 97-104.
Sampaio, I. B. M. (1998). Estatística aplicada a experimentação animal. Belo Horizonte, MG, Brasil.
Santman-Berends, I. M. G. A., Swinkels, J. M., Lam, T. J. G. M., Keurentjes, J., & Schaik, G. van. (2016). Evaluation of udder health parameters and risk factors for clinical mastitis in Dutch dairy herds in the context of a restricted antimicrobial usage policy. Journal of Dairy Science, 99(4), 2930-2939. doi: 10.3168/jds.2015-10398.
Schmidt, T., Kock, M., & Ehlers, M. (2015). Diversity and antimicrobial susceptibility profiling of staphylococci isolated from bovine mastitis cases and close human contacts. Journal of Dairy Science, 98(9), 6256-6269. doi: 10.3168/jds.2015-9715
Casanova, V. P., Appio, J., Kohl, E., Michaelsen, T. R., Paim, S. D., Brunetto, T. R. Girardini, L. (2016). Bovine mastitis: prevalence and antimicrobial susceptibility profile and detection of genes associated with biofilm formation in Staphylococcus aureus. Semina: Ciências Agrárias, 37(3), 1369-1378. doi: 10.5433/1679-0359.2016v37n3p1369
Clinical & Laboratory Standards Institute (2018). Performance standards for antimicrobial disk susceptibility tests. Wayne: West Valley Road.
Freitas, C. H., Mendes, J. F., Villarreala, P. V., Santos, P. R., Gonçalves, C. L., Gindonis, V., Rantala, M. (2018). Occurrence and characterization of methicillin-resistant staphylococci from bovine mastitis milk samples in Finland. Acta Veterinaria Scandinavica, 55(1), 61. doi: 10.1186/1751-0147-55-61
Ganda, E. K., Bisinotto, R. S., Decter, D. H., & Bicalho, R. C. (2016). Evaluation of an on-farm culture system (accumast) for fast identification of milk pathogens associated with clinical mastitis in dairy cows. PLoS One, 11(5), 1-16. doi: 10.1371/journal.pone.0155314.
Israel, L. F. S., Rabello, R. F., Domingos, S. C. B., & Medeiros, L. S. (2018). Biofilm production by Staphylococcus chromogenes isolated from milk samples from bovine with mastitis. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 70(6), 1943-1949. doi: 10.1590/1678-4162-9866
Mello, P. L., Pinheiro, L., Martins, L. de A., Brito, M. A. V. P., Cunha, R. de S., & Cunha, M. de L. R. de S. (2017). Short communication: B Lactam resistance and vancomycin heteroresistance in Staphylococcus spp. isolated from bovine subclinical mastitis. Journal of Dairy Science, 100(8), 6567-6571. doi: 10.3168/jds.2016-12329
Mesquita, A. A. (2017). Impacto da mastite bovina por Sthapylococcus aureus e Streptococcus agalactiae. Tese de mestrado, Universidade Federal de Lavras. Lavras, MG, Brasil. Recuperado de http://repositorio.ufla.br/jspui/bitstream/1/28358/2/TESE_Impacto%20da%20Mastite%20bovina%20por%20Sthapylococcus%20aureus%20e%20Streptococcus%20agalactiae.pdf
Moritz, F., & Moritz, C. M. F. (2016). Resistência aos antimicrobianos em Staphylococcus spp. associados à mastite bovina. Revista de Ciência Veterinária e Saúde Pública, 3(2), 132-136. doi: 10.4025/revcivet.v3i2.34435
National Mastitis Council (2004). Procedures for collecting milk samples. Recuperado de https://www.nmconline.org/nmc-protocols-guidelines-and-procedures/.
Oliveira, C. S. F. (2015). Análise epidemiológica e bioeconômica da mastite bovina em rebanhos brasileiros. Tese de doutorado, Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil. Recuperado de https://repositorio.ufmg.br/bitstream/1843/BUBD-A5GMBM/1/texto_tese_final___camila_stefanie_ fonseca_de_oliveira.pdf
Papadopoulos, P., Papadopoulos, T., Angelidis, A. S., Boukouvala, E., Zdragas, A., Papa, A. Sergelidis, D. (2018). Prevalence of Staphylococcus aureus and of methicillin-resistant S. aureus (MRSA) along the production chain of dairy products in north-western Greece. Food Microbiology, 69,43-50. doi: 10.1016/j.fm.2017.07.016
Queiroz, A. M., & Souza, L. G. S. (2021). Análise da produção de leite de vaca no Estado do Acre. Scientia Naturalis, 3(1), 97-104.
Sampaio, I. B. M. (1998). Estatística aplicada a experimentação animal. Belo Horizonte, MG, Brasil.
Santman-Berends, I. M. G. A., Swinkels, J. M., Lam, T. J. G. M., Keurentjes, J., & Schaik, G. van. (2016). Evaluation of udder health parameters and risk factors for clinical mastitis in Dutch dairy herds in the context of a restricted antimicrobial usage policy. Journal of Dairy Science, 99(4), 2930-2939. doi: 10.3168/jds.2015-10398.
Schmidt, T., Kock, M., & Ehlers, M. (2015). Diversity and antimicrobial susceptibility profiling of staphylococci isolated from bovine mastitis cases and close human contacts. Journal of Dairy Science, 98(9), 6256-6269. doi: 10.3168/jds.2015-9715
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Published
2022-02-25
How to Cite
Ferreira, L. B., Israel, L. de F. S., Rabello, R. F., Souza, G. N. de, Peruquetti, R. C., & Medeiros, L. dos S. (2022). Risk factors associated with the occurrence of multiresistant Staphylococcus spp. isolated from bovine subclinical mastitis in northern Brazil. Semina: Ciências Agrárias, 43(2), 901–910. https://doi.org/10.5433/1679-0359.2022v43n2p901
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