Detection of mycobacteria in coalho cheese sold in Northeastern Brazil

Authors

DOI:

https://doi.org/10.5433/1679-0359.2021v42n1p439

Keywords:

Coalho cheese. Mycobacterium lehmanii. Mycobacterium ssp. Mycobacterium rutilum, Nested-PCR.

Abstract

The aim of the present study was to detect and identify Mycobaterium spp. in 50 samples of coalho cheese sold in the Northeast region of Brazil. Of the 50 analyzed samples, 35 were produced by the artisanal process, using raw milk, and 15 originated from industrialized establishments that pasteurize milk. Conventional and real-time nested PCR for the rv2807 gene of the M. tuberculosis complex was performed directly from the 50 analyzed samples. Samples of coalho cheese were grown simultaneously in Stonebrink medium, and conventional PCR was performed from the bacterial isolates with primers that flank differentiation region 4 (DR4), specific to M. bovis, mb400F. Bacterial isolates negative by PCR for RD4 were subjected to PCR for hsp65 of Mycobacterium spp., with subsequent DNA sequencing. The cultures were negative for the M. tuberculosis complex, but two samples (4%) from the artisanal process (with raw milk) exhibited identity with hsp65 of Mycobacterium lehmanii (Sequence ID: KY933786.1, identities: 312/363 [86%]); and Mycobacterium rutilum (sequence ID: LT629971.1, identities: 331/371 [89%]), showing to be indicative environmental contamination. Non-tuberculous mycobacteria are emergent and ubiquitous microorganisms; therefore, they deserve greater attention in the cheese production chain, both in terms of Good Agricultural Practices (GAP) and food Good Manufacturing Practices (GMP).

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Author Biographies

Giovanni Brito Medeiros, Universidade Federal de Campina Grande

Discente do Curso de Doutorado do Programa de Pós-Graduação em Ciência Animal, PPGCA, Mestrado, Universidade Federal de Campina Grande, UFCG, Patos, PB, Brasil.

Onaldo Guedes Rodrigues, Universidade Federal de Campina Grande

Prof. Dr., UFCG, Patos, PB, Brasil.

Pirajá Saraiva Bezerra Neto, Universidade Federal de Campina Grande

Discente do Curso de Doutorado do Programa de Pós-Graduação em Ciência Animal, PPGCA, Mestrado, Universidade Federal de Campina Grande, UFCG, Patos, PB, Brasil.

Rosália Severo de Medeiros, Universidade Federal de Campina Grande

Profa Dra, UFCG, Patos, PB, Brasil.

Elaine Cristina Fernandes Baez Sarti, Empresa Brasileira de Pesquisa Agropecuária

Pesquisadora, Empresa Brasileira de Pesquisa Agropecuária, EMBRAPA Gado de Corte, Campo Grande, MS, Brasil.

Flábio Ribeiro de Araújo, Empresa Brasileira de Pesquisa Agropecuária

Pesquisador, Empresa Brasileira de Pesquisa Agropecuária, EMBRAPA Gado de Corte, Campo Grande, MS, Brasil.

Sérgio Santos de Azevedo, Universidade Federal de Campina Grande

Prof. Dr., UFCG, Patos, PB, Brasil.

Severino Silvano dos Santos Higino, Universidade Federal de Campina Grande

Prof. Dr., UFCG, Patos, PB, Brasil.

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Published

2021-01-19

How to Cite

Medeiros, G. B., Rodrigues, O. G., Bezerra Neto, P. S., Medeiros, R. S. de, Sarti, E. C. F. B., Araújo, F. R. de, … Higino, S. S. dos S. (2021). Detection of mycobacteria in coalho cheese sold in Northeastern Brazil. Semina: Ciências Agrárias, 42(1), 439–446. https://doi.org/10.5433/1679-0359.2021v42n1p439

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Section

Communication

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