Investigation of Vibrio cholerae, V. parahaemolyticus, and V. vulnificus in clandestine animal products and water effluent samples in northern Tocantins, Brazil
DOI:
https://doi.org/10.5433/1679-0359.2024v45n5p1681Keywords:
Public health, PCR, Foodborne diseases, Food safety.Abstract
Vibrio spp. are responsible for several diseases, including cholera, which affect public and environmental health. Waterborne and foodborne diseases can cause serious harm to the society because of their potential to infect large numbers of people in a short time. The presence or absence of pathogenic microorganisms in a wide variety of samples, whether in the food, health, or environmental domains, has become possible because of the spread of molecular biology-based approaches. Compared to conventional culture methods, these methodologies have advantages, mainly in terms of sensitivity and relative high speed of analysis. Considering the risk to public health, and given the importance of the range of diseases caused by these pathogens, the aim of this project was to identify Vibrio cholerae, V. parahaemolyticus, and V. vulnificus among bacterial isolates from clandestine animal products commercialized in northern Tocantins and from water effluents from the Lontra River in Araguaína. In total, 199 isolates from animal products and 366 from water samples were evaluated. Among them, 25 and 78 isolates from animal products and water samples were confirmed as Vibrio spp. using uniplex PCR, respectively, and 10 of them were sequenced. The sequencing results questioned the specificity of the biomolecular assays performed given that the isolates were identified as Aeromonas spp., Escherichia coli, and Morganella spp. Under the tested conditions, performed assays can lead to false-positive results for Vibrio spp.
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