Evaluation of the fungus Duddingtonia flagrans (Bioverm®) on Ascaris suum eggs and infective larvae of Oesophagostomum spp. and Hyostrongylus rubidus from swine
DOI:
https://doi.org/10.5433/1679-0359.2023v44n4p1587Keywords:
Ascarides, Biological control, Gastrointestinal strongyles, Nematophagous fungi, Pigs.Abstract
The aim of this study was to evaluate the action of a commercial formulation based on Duddingtonia flagrans (Bioverm®) on Ascaris suum eggs and infective larvae (L3) of Oesophagostomum spp. and Hyostrongylus rubidus from swine. Twelve male pigs were divided into two groups: treated, which received a single dose of 1 g/10 kg of body weight (105 chlamydospores of D. flagrans); and control, which remained untreated. Fecal samples (100g) were collected individually at 0, 12, 24, 36, 48, 60 and 72 hours after treatments. In the assay A, 2 g of feces and 1000 eggs of A. suum were added to petri dishes, and larval predation was assessed to classify the effects of predation. In the assay B, 2000 L3 of Oesophagostomum spp. and H. rubidus were added to the coprocultures, incubated for 10 days and recovered for quantification of non-predated L3. In the assay A, there was only a type 1 effect, with fungal growth in eggs, but no embryo damage. In the assay B, a reduction of L3 of Oesophagostomum spp. and H. rubidus of 73.9% (p<0.01), with predation peak 24 h after product administration. It was concluded that D. flagrans (Bioverm®) only had predatory efficacy on L3 of swine gastrointestinal strongyles.
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