Presence of leaves and use of IBA in adventitious rooting, chlorophyll fluorescence, and root ontogeny of 'Fepagro C 13' and 'Trifoliata' cuttings
DOI:
https://doi.org/10.5433/1679-0359.2022v43n5p2171Keywords:
Photochemical efficiency; Rootstock; Vascular cambium; Vegetative propagation.Abstract
Cutting is proposed as an alternative method for the propagation of citrus rootstocks to obtain clonal material especially in varieties with a low rate of polyembryony—and possibly reduce the seedling formation period. The aim of this study was to examine the influence of leaves and the application of indolebutyric acid (IBA) on adventitious rooting and chlorophyll fluorescence as well as to characterize root ontogeny in 'Fepagro C 13' and 'Trifoliata' citrus rootstock cuttings. Semi-hardwood cuttings were collected in December 2018 and subjected to the following treatments: presence or absence of leaves, with or without application of IBA (4000 mg L-1). Over 92 days in a greenhouse environment, leaf abscission percentage, cutting mortality, rooting percentage, and number of roots per cutting were evaluated. Additionally, initial (Fo), maximum (Fm), and variable (Fv) chlorophyll fluorescence, photochemical efficiency (Fv/Fm), and Fv/Fo ratio were analyzed. Tissue samples from the base of the cutting were collected for the anatomical study. The presence of leaves on 'Fepagro C 13' and 'Trifoliata' cuttings is essential for adventitious rooting. The use of auxin influences the photochemical efficiency of leaves on the cuttings. In 'Trifoliata' cuttings, adventitious roots originate from the vascular cambium.
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