Molecular survey of Cryptosporidium spp. in calves from the state of Mato Grosso, Brazil

Authors

DOI:

https://doi.org/10.5433/1679-0359.2020v41n5supl1p2437

Keywords:

Calf, Cryptosporidium parvum, Feces, Zoonosis.

Abstract

Cryptosporidium spp. is a protozoan that infects a wide range of vertebrate hosts; it has been reported to be the cause of severe illness or death in livestock worldwide, which leads to decreased performance and production losses, especially in young animals. This study investigated the presence of Cryptosporidium in calves from beef farms in the state of Mato Grosso, midwestern Brazil. For this purpose, fecal samples from 237 animals aged ? 45 days, raised in 20 rural properties were subjected to DNA extraction and nested polymerase chain reaction (nPCR) targeting 18S ribosomal RNA (18S rRNA) gene followed by sequencing. Additionally, positive samples, previously identified as Cryptosporidium parvum by sequencing and phylogenetic analyses based on 18S rRNA gene, were subsequently analyzed focusing the amplification and sequencing using nPCR of a fragment of the 60 kDa glycoprotein (gp60) gene. Of the 237 fecal samples analyzed by PCR (18S rRNA), 50 (21.1%) fecal samples were positive for Cryptosporidium spp., while 14 (70%) of the 20 properties had at least one positive animal. The following Cryptosporidium species were detected: C. bovis, C. parvum, and C. ryanae. Thereafter, two potentially zoonotic subtypes (IIaA15G2R1 and IIaA16G3R1) of C. parvum were identified based on gp60 gene sequences. This study resulted in the detection of subtype IIaA16G3R1 for the first time in South America and showed a wide distribution of the protozoan in beef farms in the studied area of the State.

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Author Biographies

Thábata dos Anjos Pacheco, Universidade Federal do Mato Grosso

Discente do Curso de Doutorado, Programa de Pós-Graduação em Ciências Veterinária, Faculdade de Medicina Veterinária, Universidade Federal do Mato Grosso, UFMT, Cuiabá, MT, Brasil.

Felippe Danyel Cardoso Martins, Universidade Estadual de Londrina

Discente do Curso de Doutorado, Programa de Pós-Graduação em Ciência Animal, Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, UEL, Londrina, PR, Brasil.

Sayanne Luns Hatum de Almeida, Universidade Federal do Mato Grosso

Discente do Curso de Doutorado, Programa de Pós-Graduação em Ciências Veterinária, Faculdade de Medicina Veterinária, Universidade Federal do Mato Grosso, UFMT, Cuiabá, MT, Brasil.

Thiago Borges Fernandes Semedo, Instituto Nacional de Pesquisa do Pantanal

Pesquisador, M.e, Programa de Capacitação Institucional, Museu Paraense Emílio Goeldi, Instituto Nacional de Pesquisa do Pantanal, INPP, Cuiabá, MT, Brasil

Michelle Igarashi Watanabe, Universidade Federal do Mato Grosso

Profa Dra, Departamento de Ciências Básicas em Saúde, Faculdade de Medicina, UFMT, Cuiabá, MT, Brasil.

Valéria Dutra, Universidade Federal do Mato Grosso

Profa Dra, Programa de Pós-Graduação em Ciências Veterinária, UFMT, Cuiabá, MT, Brasil.

João Luis Garcia, Universidade Estadual de Londrina

Prof. Dr., Programa de Pós-Graduação em Ciência Animal, Departamento de Medicina Veterinária Preventiva, Centro de Ciências Agrárias, UEL, Londrina, PR, Brasil.

Richard Campos Pacheco, Universidade Federal do Mato Grosso

Prof. Dr., Programa de Pós-Graduação em Ciências Veterinária, UFMT, Cuiabá, MT, Brasil.

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Published

2020-08-07

How to Cite

Pacheco, T. dos A., Martins, F. D. C., Almeida, S. L. H. de, Semedo, T. B. F., Watanabe, M. I., Dutra, V., … Pacheco, R. C. (2020). Molecular survey of Cryptosporidium spp. in calves from the state of Mato Grosso, Brazil. Semina: Ciências Agrárias, 41(5supl1), 2437–2444. https://doi.org/10.5433/1679-0359.2020v41n5supl1p2437

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Section

Communication

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