Microbiological evaluation of lettuce produced by conventional and organic systems in farms of Londrina, PR

Authors

  • Natalia Harumi Niguma Universidade Estadual de Londrina
  • Jacinta Sanchez Pelayo Universidade Estadual de Londrina
  • Tereza Cristina Rocha Moreira de Oliveira Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0359.2017v38n1p175

Keywords:

Coliform at 45 °C, Total coliform, Salmonella spp, Irrigation water.

Abstract

The aims of this study were to evaluate the contamination of lettuce (Lactuca sativa), produced in Londrina, Paraná (PR), with total coliform, coliform at 45 °C, E. coli, and Salmonella spp.; and to determine the E. coli contamination of irrigation water used at the farms studied. Four farms were evaluated, of which three produced lettuce using a conventional system and one using an organic system. An evaluation of the production practices of the farms was also carried out. A total of 111 samples were analyzed, 71 lettuce samples from the conventional system and 40 samples from the organic system. A total of eight irrigation water samples were collected for analysis. Coliform at 45 °C counts above the limit tolerated by Brazilian legislation were observed in 2.8% (2/71) of conventionally grown lettuce samples, and Salmonella spp. was isolated in 1.4% (1/71) of those samples. In the organic lettuce samples, 12.5% (5/40) had coliform at 45 °C counts above the limit tolerated and Salmonella spp. was not detected. Irrigation water samples from three farms were unsatisfactory, with counts higher than 102MPN of E. coli per 100mL. The results of this study demonstrate that most conventionally grown lettuce samples show good sanitary conditions in production, and that lettuce contamination is not related to contamination found in irrigation water samples. The results also showed that the organic production practices required by Brazilian certification agencies should be applied to ensure that contamination of produced lettuce remains controlled.

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Author Biographies

Natalia Harumi Niguma, Universidade Estadual de Londrina

Discente do Curso de Doutorado do Programa de Pós-Graduação em Ciência de Alimentos, Departamento de Ciência e Tecnologia de Alimentos, Universidade Estadual de Londrina, UEL, Londrina, PR, Brasil.

Jacinta Sanchez Pelayo, Universidade Estadual de Londrina

Profª Drª, Laboratório de Bacteriologia, Departamento de Microbiologia do Centro de Ciências Biológicas, UEL, Londrina, PR.

Tereza Cristina Rocha Moreira de Oliveira, Universidade Estadual de Londrina

Profª Drª, Departamento de Ciência e Tecnologia de Alimentos, UEL, PR.

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Published

2017-03-02

How to Cite

Niguma, N. H., Pelayo, J. S., & Oliveira, T. C. R. M. de. (2017). Microbiological evaluation of lettuce produced by conventional and organic systems in farms of Londrina, PR. Semina: Ciências Agrárias, 38(1), 175–184. https://doi.org/10.5433/1679-0359.2017v38n1p175

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