Optimization of protein extraction from the yeast Saccharomyces cerevisiae

Authors

  • Antonio Martins Oliveira do Instituto Municipal de Ensino Superior de Assis
  • Raul Jorge Hernan C. Gómez Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0359.2005v26n4p521

Keywords:

Yeast, Extraction of proteins, Autolysis.

Abstract

This work aimed to determine the optimum temperature, pH and sodium chloride sodium concentration for protein extraction of yeast cells during autolysis process. The cellular extract was obtained using commercial compressed baker’s yeast Saccharomyces cerevisiae and for statistical analysis and definition of the variation levels of temperature (32,0 to 52,0°C), pH (1,32 to 7,00) and NaCl (2,0 to 75%) the Response Surface Analysis Methodology was used. The result obtained showed that the best extraction conditions were: temperature between 49,0 and 51,0°C combined with pH values between 3,8 and 5,0 and sodium chloride concentration between 10,0 and 12,0% (w/v), however, sodium chloride concentration higher than 12% was not recommended.

Author Biographies

Antonio Martins Oliveira, do Instituto Municipal de Ensino Superior de Assis

Professor do Curso de Química Industrial do Instituto Municipal de Ensino Superior de Assis – Fema/Imesa - Assis – SP.

Raul Jorge Hernan C. Gómez, Universidade Estadual de Londrina

Professor Orientador do Departamento de Tecnologia de Alimentos e Medicamentos, Centro de Ciências Agrárias/CCA -

Universidade Estadual de Londrina. Caixa Postal 6001, CEP 86051-970 – Londrina. PR.

 

Published

2005-06-30

How to Cite

Oliveira, A. M., & Gómez, R. J. H. C. (2005). Optimization of protein extraction from the yeast Saccharomyces cerevisiae. Semina: Ciências Agrárias, 26(4), 521–534. https://doi.org/10.5433/1679-0359.2005v26n4p521

Issue

Section

Articles