Development of a method for determination of fatty acid using FT-IR spectroscopy

Development of a method for determination of fatty acid using FT-IR spectroscopy

Authors

  • Dimas Augusto Morozin Zaia Universidade Estadual de Londrina
  • Leonardo de Oliveira Guarnieri Universidade Estadual de Londrina
  • Karina Nicole Sobota Universidade Estadual de Londrina
  • Henrique de Santana Universidade Estadual de Londrina
  • Cássia Thaïs Bussamra Vieira Zaia Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0375.2011v32n1p79

Keywords:

Fatty acids, Palmitic acid, Biological systems, FT-IR Spectroscopy

Abstract

In the present paper a new methodology has been developed for determination of fatty acids in biological systems using FT-IR spectroscopy. For this method is not necessary chromophore reagent or pre sample preparation. Palmitic acid was chosen as standard, because it is found in several biological systems. The FT-IR spectrum of palmitic acid showed two absorption bands in the region of 2852 and 2920 cm-1 attributed to CH stretching. The results for these bands showed that the Beer-Lambert Law was followed in wide range of concentration of palmitic acid (14 to 257 mmol L-1). Potassium ferricyanide (K3[Fe(CN)6]) was used as internal standard. Several interferents were tested and only cholesterol, ferric chloride (higher concentration), mixture of amino acids for the band at 2919 cm-1 (higher concentration) and triglyceride could be interferent if they appear in high concentration. Thus, this new methodology has advantage to be not expensive and simple.

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Author Biographies

Dimas Augusto Morozin Zaia, Universidade Estadual de Londrina

Professor Associado-C, Departamento de Química-CCE

Leonardo de Oliveira Guarnieri, Universidade Estadual de Londrina

Bacharelando em Biomedicina, Departamento de Ciências Fisiológicas-CCB

Karina Nicole Sobota, Universidade Estadual de Londrina

Bacharelando em Biomedicina, Departamento de Ciências Fisiológicas-CCB

Henrique de Santana, Universidade Estadual de Londrina

Professor Associado-C, Departamento de Química-CCE

Cássia Thaïs Bussamra Vieira Zaia, Universidade Estadual de Londrina

 

 Professora Associada-C, LaFiNeM-Laboratório de Fisiologia Neuroendócrina e metabolismo, Departamento de Ciências Fisiológicas-CCB, Universidade Estadual de Londrina

Published

2011-07-15

How to Cite

Zaia, D. A. M., Guarnieri, L. de O., Sobota, K. N., de Santana, H., & Zaia, C. T. B. V. (2011). Development of a method for determination of fatty acid using FT-IR spectroscopy. Semina: Ciências Exatas E Tecnológicas, 32(1), 79–86. https://doi.org/10.5433/1679-0375.2011v32n1p79

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Original Article

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