Gallus gallus domesticus (Galliformes: Phasianidae) as a biological model for biosafety analysis of the hexane fraction of the fruits and seeds of Ricinus communis L. (Malpighiales: Euphorbiaciae)
DOI:
https://doi.org/10.5433/1679-0359.2024v45n6p1785Keywords:
Ecotoxicology, Botanical insecticide, Castor bean, Toxicity.Abstract
Brazil became the largest consumer of pesticides in the world in 2021. These products, although necessary, can cause significant damage to non-target organisms and agroecosystems. From this perspective, the search for new, safer products gains prominence every year. It is essential to understand how non-target organisms react to contact with these novel products to verify their safety. Therefore, vertebrate models, such as the embryo of Gallus gallus domesticus L., 1758 (Galliformes: Phasianidae), an organism from which the results can be extrapolated to other vertebrates, are important to guarantee the selectivity and safety of new products. The objective of this study was to evaluate the effect of the hexane fraction of fruits and seeds of Ricinus communis (HFFSRc) on Gallus gallus domesticus (the domestic chicken). This fraction has been studied as a potential insecticide for agricultural use. Three concentrations of HFFSRc were evaluated, 1%, 1.5%, and 2%, in addition to two control treatments, one with distilled water and one with Tween 80® (0.01%). The effect of HFFSRc on G. gallus domesticus embryos was analyzed through two different forms of exposure: i) injection of treatments into the egg’s air chamber and ii) spraying of treatments on the egg. After three days of incubation, the eggs of G. gallus domesticus were collected for morphological analysis using the total mount technique. It was observed that their exposure to HFFSRc, regardless of the concentration or form of exposure used, reduced the survival probability of G. gallus domesticus embryos. HFFSRc was found to be toxic to G. gallus domesticus embryos when sprayed on the eggshell, reducing their survival probability. HFFSRc was also toxic when injected into the air chamber, in addition to causing body malformations in G. gallus domesticus embryos.
Downloads
References
Allein, C. M. (2021). Seletividade do extrato hexânico de frutos de Ricinus communis L. (Euphorbiaceae) à Trichogramma pretiosum Riley, 1879 (Hymenoptera: Trichogrammatidae). Dissertação de Mestrado, Universidade Tecnológica Federal do Paraná, Dois Vizinhos, PR, Brasil.
Allein, C. M., Quisini, R., Rodrigues, M. G., Bordin, T. A., Warmling, J. V., Battisti, L., Cadorin Oldoni, T. L., Potrich, M., & Lozano, E. R. (2024). Is the hexane fraction of the extract of Ricinus communis L. (Euphorbiaceae) fruits and seeds selective for Trichogramma pretiosum Riley, 1879 (Hymenoptera: Trichogrammatidae)? Crop Protection, 184, 106839. doi: 10.1016/j.cropro.2024.106839 DOI: https://doi.org/10.1016/j.cropro.2024.106839
Almeida, F. C. G., & Lemonica, I. P. (2000). The toxic effects of Coleus barbatus B. on the different periods of pregnancy in rats. Journal of Ethnopharmacology, 73(1-2), 53-60. doi: 10.1016/S0378-8741(00)00275-0 DOI: https://doi.org/10.1016/S0378-8741(00)00275-0
Aspé, E., & Fernández, K. (2011). The effect of different extraction techniques on extraction yield, total phenolic, and anti-radical capacity of extracts from Pinus radiata bark. Industrial Crops and Products, 34(1), 838-844. doi: 10.1016/j.indcrop.2011.02.002 DOI: https://doi.org/10.1016/j.indcrop.2011.02.002
Barbosa, F. S. (1995). Tópicos em malacologia médica. Editora FIOCRUZ. DOI: https://doi.org/10.7476/9788575414019
Blaszczyk, A., Matysiak, S., Kula, J., Szostakiewicz, K., & Karkusiewicz, Z. (2020). Cytotoxic and genotoxic effects of (R)- and (S)-ricinoleic acid derivatives. Chirality, 32(7), 998-1007. doi: 10.1002/chir.23226 DOI: https://doi.org/10.1002/chir.23226
Bordin, T. A., Henning, L. de L., Rodrigues, M. G., Oldoni, T. L. C., Carvalho, G. A., Potrich, M., & Lozano, E. R. (2023). Toxicity of the hexane fraction of fruits and seeds of Ricinus communis to caterpillars of the Spodoptera complex. Agriculture, 13(6), 1124. doi: 10.3390/agriculture13061124 DOI: https://doi.org/10.3390/agriculture13061124
Borges, L. P., & Amorim, V. A. (2020). Metabólitos secundários de plantas. Revista Agrotecnologia, 11(1), 54-67.
Caldas, G. F. R., Dantas, L. P., Camurça, A. J. dos S., Teixeira, M. M. de S., Rodrigues, F. F. G., Costa, J. G. M. da, & Wanderley, A. G. (2020). Propriedades farmacológicas e toxicologia do óleo essencial de Hyptis martiusii Benth.(cidreira-brava) e de seu composto majoritário 1, 8-cineol: Uma Revisão. Revista Interfaces: Saúde, Humanas e Tecnologia, 8(1), 461-471. doi: 10.16891/2317-434x.v8.e1.a2020.pp461-471 DOI: https://doi.org/10.16891/2317-434X.v8.e1.a2020.pp461-471
Carlson, B. M. (2014). Embriologia humana e biologia do desenvolvimento (5nd ed.). GEN Guanabara Koogan.
Corder, G. W., & Foreman, D. I. (2011). Nonparametric statistics for non‐statisticians. Wiley.
Cruz, M. P. da, Larentis, L. T., Vismara, E. de S., Vismara, L. de S., Freitas, P. F. de, & Mazaro, S. M. (2021). Action of Ganoderma lucidum mycelial growth filtrates on Erysiphe diffusa and embryotoxicity assessment in a chicken embryo model. Acta Biologica Szegediensis, 65(1), 47-57. doi: 10.14232/abs.2021.1.47-57 DOI: https://doi.org/10.14232/abs.2021.1.47-57
Cunha, M. A. S. (2006). Análise molecular da variabilidade genética entre genótipos de Ricinus communis L. revelada por marcadores RAPD. Dissertação de Mestrado, Universidade Federal de Alagoas, Rio Largo, AL, Brasil.
Dantas, P. C., Araújo, R. G. V. de, Abreu, L. A. de, Araújo, J. V. de A., Jr., Batista, A. S., Sabino, A. R., & Cunha, J. L. X. L. (2019). Toxicidade de extratos vegetais em Coccidophilus citrícola (Brèthes, 1905) (Coleoptera: Coccinellidae). Brazilian Journal of Development, 5(3), 2060-2067. doi: 10.34117/bjdv5n3-1217
Dicke, J. M. (1989). Teratology: principles and practice. Medical Clinics of North America, 73(3), 567-582. doi: 10.1016/S0025-7125(16)30658-7 DOI: https://doi.org/10.1016/S0025-7125(16)30658-7
Figueiredo, E. M. de, Corrêa, G. da S. S., Albino, L. F. T., Donzele, R. F. M. de O., Donzele, J. L., Pinto, R., da Silva, M. D., Corrêa, A. B., & Tavares, J. M. N. (2021). Fisiologia da formação do ovo: um referencial teórioco. In R. L Galati, & M. F. S. Quieroz (Eds.), Inovações na nutrição animal: Desafios da produção de qualidade (pp. 109-126). Editora Científica Digital. DOI: https://doi.org/10.37885/210504516
Galvão, G. C., Lopes, J. M. H., Bueno, L. M., & Freitas, P. F. de. (2019). Avaliação dos efeitos de extrato vegetal de Cymbopogon winterianus sobre o desenvolvimento embrionário inicial de ave. In C. T. Slivinski (Ed.), Impactos das tecnologias nas ciências biológicas e da saúde 3 (pp. 144-149). Belo Horizonte. DOI: https://doi.org/10.22533/at.ed.37719160116
Gonzales, E., & Macari, M. (2003). Manejo da incubação. FACTA.
Hamburger, V., & Hamilton, H. L. (1951). A series of normal stages in the development of the chick embryo. Journal of Morphology, 88(1), 49-92. doi: 10.1002/aja.1001950404 DOI: https://doi.org/10.1002/jmor.1050880104
Hill, M. A. (2023). Embryology Carnegie stages. UNSW Embryology. https://embryology.med.unsw.edu.au/embryology/index.php/Carnegie_Stages.
Irie, N., & Kuratani, S. (2011). Comparative transcriptome analysis reveals vertebrate phylotypic period during organogenesis. Nature Communications, 2(1), 1248. doi: 10.1038/ncomms1248 DOI: https://doi.org/10.1038/ncomms1248
Kmecick, M. R. D. (2017). Avaliação dos efeitos do cádmio e ácido perfluorooctanóico nos estágios iniciais de desenvolvimento de ave (Gallus gallus). DSpace. https://acervodigital.ufpr.br/handle/1884/56771
Korn, M., & Cramer, K. (2007). Windowing chicken eggs for developmental studies. Journal of Visualized Experiments, 8, e306. doi: 10.3791/306 DOI: https://doi.org/10.3791/306
Larentis, L. T., Santos, T. dos, Oliveira, A. de, & Freitas, P. F. de. (2019). Avaliação dos efeitos de Beauveria bassiana (Hypocreales: Cordycipitaceae) e óleos essencias de Pogostemon cablin (Lamiales: Lamiaceae) sobre o desenvolvimento embrionario inicial de Gallus gallus (Galliformes: Phasianidae). In C. T. Slivinski (Ed.), Impactos das tecnologias nas ciências biológicas e da saúde 3 (pp. 130-134). Belo Horizonte. DOI: https://doi.org/10.22533/at.ed.37719160114
Léonard, A., Clément, S., Kuo, C. D., & Manto, M. (2019). Changes in heart rate variability during heartfulness meditation: a power spectral analysis including the residual spectrum. Frontiers in Cardiovascular Medicine, 6, 1-8. doi: 10.3389/fcvm.2019.00062 DOI: https://doi.org/10.3389/fcvm.2019.00062
Macedo, T., Paiva-Martins, F., Valentão, P., & Pereira, D. M. (2024). In silico and in vitro chemometrics, cell toxicity and permeability of naringenin 8-sulphonate and derivatives. Frontiers in Pharmacology, 15, 1-12. doi: 10.3389/fphar.2024.1398389 DOI: https://doi.org/10.3389/fphar.2024.1398389
Marangoni, C., Fernandes de Moura, N., Roberto, F., & Garcia, M. (2012). Utilização de óleos essenciais e extratos de plantas no controle de insetos. Revista de Ciências Ambientais, 6(2), 95-112.
Marcelino, A., Wachtel, C., & Ghisi, N. (2019). Are our farm workers in danger? Genetic damage in farmers exposed to pesticides. International Journal of Environmental Research and Public Health, 16(3), 358. doi: 10.3390/ijerph16030358 DOI: https://doi.org/10.3390/ijerph16030358
Martinez, E. A., Casalinho, H. D., Lima, A. C. R. de, & Schwengber, J. E. (2017). Oferta de serviços ambientais a partir de diferentes agroecossistemas de base familiar no sul do Rio Grande do Sul. Agricultura Familiar: Pesquisa, Formação e Desenvolvimento, 11(1), 71-86. doi: 10.18542/raf.v11i1.4678 DOI: https://doi.org/10.18542/raf.v11i1.4678
Mazzuco, H. (2008). Ovo: alimento funcional, perfeito à saúde. Revista Avicultura Industrial, 2, 12-16.
McCullagh, P., & Nelder, J. A. (2019). Generalized linear models. Routledge. DOI: https://doi.org/10.1201/9780203753736
Moore, K. L., Persaud, T. V. N., & Torchia, M. G. (2020). Embriología clínica (11nd ed.). Elsevier.
Péres, V. F., Saffi, J., Melecchi, M. I. S., Abad, F. C., Assis Jacques, R. de, Martinez, M. M., Oliveira, E. C., & Caramão, E. B. (2006). Comparison of soxhlet, ultrasound-assisted and pressurized liquid extraction of terpenes, fatty acids and vitamin E from Piper gaudichaudianum Kunth. Journal of Chromatography A, 1105(1-2), 115-118. doi: 10.1016/j.chroma.2005.07.113 DOI: https://doi.org/10.1016/j.chroma.2005.07.113
Sadler, T. (2012). Langman. Embriología médica. Ovid Technologies.
Saito, M. L., Pott, A., Ferraz, J. M. G., & Nascimento, R. D. S. (2004). Avaliação de plantas com atividade deterrente alimentar em Spodoptera frugiperda (J. E. Smith) e Anticarsia gemmatalis Hubner. Pesticidas: Revista de Ecotoxicologia e Meio Ambiente, 14, 1-10. https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/15512/1/2004SP30SAITOavaliacao6937.pdf DOI: https://doi.org/10.5380/pes.v14i0.3117
Santana, E. C., Pires, L. B., Quintans, L. J., Jr., Quintans, J. de S. S., Araújo, A. A. de S., Santana, C. C., Jr., Serafini, M. R., Oliveira, A. M. S., Oliveira, T. B., & Duarte, M. C. (2022). Substâncias fitoquímicas para o controle do Aedes aegypti: Protocolo de scoping review. Research, Society and Development, 11(6), e39411629343. doi: 10.33448/rsd-v11i6. DOI: https://doi.org/10.33448/rsd-v11i6.29343
Schoenwolf, G. C. (1999). The avian embryo. In S. A. Moody (Ed.), Cell lineage and fate determination (pp. 429-436). San Diego. DOI: https://doi.org/10.1016/B978-012505255-9/50029-8
Sesti, L. A. C. (2003). Órgãos reprodutivos e reprodução de aves domésticas In E. Gonzales, & M. Macari (Eds.), Manejo da incubação (pp. 3-33). Campinas.
Snyder, L. R., Kirkland, J. J., & Glajch, J. L. (1997). Practical HPLC method development (2nd ed.). Wiley. DOI: https://doi.org/10.1002/9781118592014
Sun, Y., Liu, Z., & Wang, J. (2011). Ultrasound-assisted extraction of five isoflavones from Iris tectorum Maxim. Separation and Purification Technology, 78(1), 49-54. doi: 10.1016/j.seppur.2011.01.017 DOI: https://doi.org/10.1016/j.seppur.2011.01.017
União, Diário Oficial da (2013). Institui a Diretriz da Prática de Eutanásia do Conselho Nacional de Controle de Experimentação Animal CONCEA. Resolução Normativa, (13).
Warmling, J. V. (2018). Efeitos letais e subletais de extratos vegetais alcoólicos sobre Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae). Dissertação de Mestrado, Universidade Tecnológica Federal do Paraná, Dois Vizinhos, PR, Brasil.
Wiesbrook, M. L. (2004). Natural indeed: are natural insecticides safer and better than conventional insecticides? Illinois Pesticide Review, 17(3), 1-3.
Yamamoto, F. Y., Filipak, F., Neto, Freitas, P. F., Oliveira Ribeiro, C. A., & Ortolani-Machado, C. F. (2012). Cadmium effects on early development of chick embryos. Environmental Toxicology and Pharmacology, 34(2), 548-555. doi: 10.1016/j.etap.2012.06.010 DOI: https://doi.org/10.1016/j.etap.2012.06.010
Yeboah, A., Ying, S., Lu, J., Xie, Y., Amoanimaa-Dede, H., Boateng, K. G. A., Chen, M., & Yin, X. (2021). Castor oil (Ricinus communis): a review on the chemical composition and physicochemical properties. Food Science and Technology, 41(Suppl. 2), 399-413. doi: 10.1590/fst.19620 DOI: https://doi.org/10.1590/fst.19620
Zhang, G., He, L., & Hu, M. (2011). Optimized ultrasonic-assisted extraction of flavonoids from Prunella vulgaris L. and evaluation of antioxidant activities in vitro. Innovative Food Science & Emerging Technologies, 12(1), 18-25. doi: 10.1016/j.ifset.2010.12.003 DOI: https://doi.org/10.1016/j.ifset.2010.12.003
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Juliana Marceli Hofma Lopes, Patricia Franchi de Freitas, Everton Ricardi Lozano, Edgar de Souza Vismara, Elizabete Artus Berté, Silvane Zancanaro de Oliveira, Camila Maria Zankanol Griebeler, Michele Potrich
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Semina: Ciências Agrárias adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.
