Immune response to Massachusetts and Brazilian variant strains of infectious bronchitis virus in broiler chickens
DOI:
https://doi.org/10.5433/1679-0359.2024v45n4p1095Keywords:
Histomorphometry, histopathology, Humoral response, Post-vaccinal reaction.Abstract
This study aimed to assess the virulence and immune response in broiler chickens vaccinated with the Massachusetts H120 strain and the BR Variant strain of bronchitis via serological, histomorphometric, and histopathologic analyses of the tracheal mucosa. We used 162 one-day-old chicks, housed in random blocks within two temperature and light-controlled enclosures. Both the Massachusetts H120 and BR Variant strains were administered via ocular and spray routes. Blood samples were collected at four, seven, 14, 21, and 28 days of age for humoral response analysis using ELISA, from which we derived an immune response profile for each vaccine group based on the geometric mean titer (GMT). Tracheal fragments were collected at four, seven, and 14 days of age and processed using standard histological techniques. Histomorphometric measurements were analyzed using ANOVA and Tukey’s post hoc test (p < 0.05). The GMTs for both H120 and BR strains across the different administration routes were comparable in terms of humoral immune response. We observed significant histomorphometric differences (p < 0.05) among the strains and vaccine administration routes. The H120 group exhibited higher lesion scores across all ages. The results indicate that the BR Variant strain elicits a lower post-vaccinal reaction and provides superior homologous protection under the challenges of poultry farming in Brazil.
References
Assayag, M. S., Jr., Rocha, P. T., Kuana, S., Pedroso, A. C., Pereira, C. E., & Chacon, J. L. (2012). Epidemiology of infectious Bronchitis and the impact in the condemnations in the slaughterhouse. Proceedings of the Symposium of Avian Corona and Pneumoviruses, Rauischholzhausen, Germany, 7.
Balestrin, E., Fraga, A. P., Ikuta, N., Canal, C. W., Fonseca, A. S. K., & Lunge, V. R. (2014). Infectious bronchitis virus in different avian physiological systems - a field study in Brazilian poultry flocks. Poultry Science, 93(8), 1922-1929. doi: 10.3382/ps.2014-03875
Borne, P. M., & Comte, S. (2003). Vacinas e vacinação na produção avícola. Ceva Sante Animale.
Câmara, S. R., Cardoso, W. M., Teixeira, R. S. C., Romão, A. A., Salles, R. P. R., Silva, E. E., Nogueira, G. C., Sousa, F. M., & Moraes, T. G. V. (2009). Avaliação de diferentes vias vacinais para vacinação contra o vírus da doença de Newcastle em aves de fundo de quintal. Arquivo Brasileiro de Medicina Veterinária de Zootecnia, 61(6), 1308-1313. doi: 10.1590/S0102-09352009000600009
Carranza, C., Astolfi-Ferreirra, C. S., Parra, S. H. S., Nuñez, L. F. N., Penzes, Z., Chacón, J. L., Sesti, L., Chacón, R. D., & Ferreira, A. J. P. (2017). Genetic characterization and analysis of infectious bronchitis virus isolated from Brazilian flocks between 2010 and 2015. Brazilian Journal of Poultry Science, 58(6), 610-623. doi: 10.1080/00071668.2017.1365116
Cavanagh, D. (2007). Coronavirus avian infectious bronchitis virus. Veterinary Research, 38(2), 281-297. doi: 10.1051/vetres:2006055
Chacón, J. L., Rodrigues, J. N., Assayag, M. S., Jr., Peloso, C., Pedroso, A. C., & Ferreira, A. J. (2011). Epidemiological survey and molecular characterization of avian infectious bronchitis virus in Brazil between 2003 and 2009. Avian Pathology, 40(2), 153-162. doi: 10.1080/03079457.2010.544641
Chacón, J. L., Vejarano, P., Souza, L., Nagy, Z., & Penzes, Z. (2016). Homologous and heterologous protection against Brazilian BR-I viruses of Infectious Bronchitis. Proceedings of the Annual Meeting the American Association of Avian Pathologists, AAAP, San Antonio, Texas, USA.
Davelaar, F. G., & Kouwenhoven, B. (1977). Influence of maternal antibodies on vaccination of chicks of different ages against infectious bronchitis. Avian Pathology, 6(1), 41-50. doi: 10.1080/03079457708418211
De Wit, J. J., Davelaar, F. G., & Braunius, W. W. (1992). Comparison of the enzyme linked immunosorbent assay, the haemagglutination inhibition test and the agar gel precipitation test for the detection of antibodies against infectious bronchitis and Newcastle disease in commercial broilers. Avian Pathology, 21(4), 651-658. doi: 10.1080/03079459208418886
Fairbrother, A., Smits, J., & Grasman, K. (2004). Avian immunotoxicology. Journal of Toxicology and Environmental Health, Part A, 7(2), 105-137. doi: 10.1080/03079459208418886
Felippe, P. A. N., da Silva, L. H. A., Santos, M. M. A. B., Spilki, F. R., & Arns, C. W. (2010). Genetic diversity of avian infectious bronchitis virus isolated from domestic chicken flocks and coronaviruses from feral pigeons in Brazil between 2003 and 2009. Avian Diseases, 54(4), 1191-1196. doi: 10.1637/9371-041510-Reg.1
International Committee on Taxonomy of Viruses - ICTV (2024). Taxonomy lineage. https://talk.ictvonline. org/ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/negrna_viruses/199/ paramyxoviridae
Jackwood, M. W., Hilt, D. A., & Callison, S. A. (2003). Detection of infectious bronchitis virus by real-time reverse transcriptase-polymerase chain reaction and identification of a quasispecies in the Beaudette strain. Avian Diseases, 47(3), 718-724. doi: 10.1637/6075
Lai, M. M. C., & Holmes, K. V. (2001). Coronaviridae: the viruses and their replication. In B. N. Fields, D. M. Knipe, P. M. Howley, & D. E. Griffin (Eds.), Fields virology (pp. 1163-1185). New York.
Macari, M., Mendes, A. A., & Nääs, L. A. (2004). Produção de frangos de corte. Viçosa.
Mendes, A. A., & Macari, M. (2005). Manejo de matrizes de corte. Viçosa.
Mendonça, J. F. P., Martins, N. R. S., Carvalho, L. B., Sá, M. E. P., & Melo, C. B. (2009). Bronquite infecciosa das galinhas: conhecimentos atuais, cepas e vacinas no Brasil. Ciência Rural, 39(8), 2559-2566. doi: 10.1590/S0103-84782009005000195
Mesquita, D., Jr., Araújo, J. A. P., Catelan, T. T. T., Souza, A. W. S., Cruvinel, W. M., Andrade, L. E. C., & Silva, N. P. (2010). Sistema imunitário – Parte II. Fundamentos da resposta imunológica mediada por linfócitos T e B. Revista Brasileira de Reumatologia, 50(5), 552-580. doi: 10.1590/S0482-50042010000500008
Montassier, H. J. (2020). Fisiopatologia do sistema imune. In A. B. Berchieri Jr, E. N. Silva, J. Di Fabio, L. Sesti, & M. A. F. Zuanaze (Eds.), Doenças das aves (pp. 467-490). Viçosa.
Montassier, M. F. S., Brentano, L., Montassier, H. J., & Richtzenhain, L. J. (2008). Genetic grouping of avian infectious bronchitis virus isolated in Brazil, based on RT-PCR/ RFLP analysis of the S1 gene. Pesquisa Veterinária Brasileira, 28(3), 190-194. doi: 10.1590/S0100-736X2008000300011
Nunes, J. E. S., Vasconcelos, A. C., Jorge, M. A., Guimarães, E. B., Paixão, T. A., Martins, N. R. S., & Resende, J. S. (2002). Estudo comparativo da virulência de amostras de vacina do vírus da doença de Newcastle em galinhas SPF por meio da análise morfométrica da espessura traqueal. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 54(4), 335-339. doi: 10.1590/S0102-09352002000400001
Sesti, L., Kneipp, C., Paranhos, R., Paulet, P., & Cazaban, C. (2013). Field safety and efficacy of a vector Marek’s/Newcastle disease vaccine (rHVT - NDV) as assessed by clinical and productive performance in a large population of commercial broilers. Proceedings of the Western Poultry Disease Conference, Sacramento, CA, United States, 62.
Tizard, I. (2014). Veterinary immunology. Elsevier.
Wakenell, P. S., Thayer, S. G., & Beard, C. W. (2016). Serologic procedures. In Williams, S. M., Dufour-Zavala, L., Jackwood M. W., Lee, M. D., Lupiani, B., Reed, W. M., Spackman, E. & Woolcock, P. R. (Eds.), A laboratory manual for the isolation, identification and characterization of avian pathogens (pp. 343-354). Jacksonville, FL.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Semina: Ciências Agrárias
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
O Copyright dos manuscritos publicados pertence ao periódico. Como são publicados em um periódico de acesso aberto, eles estão disponíveis gratuitamente, para uso privado ou para fins educacionais e não comerciais.
A revista tem o direito de fazer, no documento original, alterações referentes às normas lingüísticas, ortografia e gramática, com o objetivo de garantir as normas padrão do idioma e a credibilidade da revista. No entanto, respeitará o estilo de escrita dos autores.
Quando necessário, alterações conceituais, correções ou sugestões serão encaminhadas aos autores. Nesses casos, o manuscrito deve ser submetido a uma nova avaliação após revisão.
A responsabilidade pelas opiniões expressas nos manuscritos é inteiramente dos autores.
