Bioprospecting phosphate-solubilizing bacteria in soils with different fertility levels
DOI:
https://doi.org/10.5433/1679-0359.2024v45n1p271Keywords:
Phosphorus, Solubilization, Pantoea sp., Enterobacter sp., Klebsiella sp.Abstract
Phosphate-solubilizing bacteria (PSB) have the ability to release fixed phosphorus (P) that is adsorbed in the soil, converting insoluble phosphate into soluble forms, making it readily available for plant absorption. Occurring naturally in various environments, with soil being the main reservoir, PSB are deemed beneficial and safe for agricultural applications. Their potential lies in isolation, multiplication, and reintroduction to the rhizosphere (through inoculants, biofertilizers, biopesticides, or biostimulants) to stimulate plant growth through direct and/or indirect mechanisms. However, identifying efficient isolates adapted to different crops and cropping systems remains a key challenge. This study aimed to prospect PSB from soils of different locations and select efficient strains with high potential for agricultural use via in vitro assays, as well as to evaluate the effects of inoculation on upland rice seeds. From soil samples collected in a conserved Amazonian biome, a productive Cerrado biome agricultural area, and a degraded with exposed subsoil, located in the Cerrado biome area, 32 P-solubilizing isolates were obtained. Among these, three isolates were selected for their early onset ability to solubilize Ca and/or Fe phosphates, and for further analysis. Identified as Pantoea sp., Enterobacter sp., and Klebsiella sp., these isolates proved to be non-harmful to rice seed health, germination, and seedling emergence, additionally promoting increased root length.
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References
Berraquero, F. R., Baya, B., & Cormenzana, A. R. (1976). Establecimiento de índices para el estudio de la solubilización de fosfatos por bacterias del suelo. Ars Pharmaceutica, 17(4), 399-406. doi: 10.30827/ars
Boni, T. S., Pereira, E. I. P., Santos, A. A., Cassiolato, A. M. R., & Maltoni, K. L. (2022). Biomass residues improve soil chemical and biological properties reestablishing native species in an exposed subsoil in Brazilian Cerrado. Plos One, 17(6), 1-19. doi: 10.1371/journal.pone.0270215 DOI: https://doi.org/10.1371/journal.pone.0270215
Borham, A., Belal, E., Metwaly, M., & El-Gremy, S. (2017). Phosphate solubilization by Enterobacter cloacae and its impact on growth and yield of wheat plants. Journal of Sustainable Agricultural Sciences, 43(2), 89-103. doi: 10.21608/JSAS.2017.1013.1003 DOI: https://doi.org/10.21608/jsas.2017.1035.1004
Costa, S. V., Silva, R. S., & Magalhães, A. M., Jr. (2020). Produção de sementes. https://www.embrapa.br/agencia-de-informacao-tecnologica/cultivos/arroz/pre-producao/producao-de-sementes
Crusciol, C. A. C., Soratto, R. P., & Arf, O. (2007). Produtividade de grãos e exportação de nutrientes de cultivares de arroz irrigadas por aspersão em consequência da época de semeadura. Bragantia, 66(2), 247-257. doi: 10.1590/S0006-87052007000200008 DOI: https://doi.org/10.1590/S0006-87052007000200008
Eke, P., Kumar, A., Sahu, K. P., Wakam, L. N., Sheoran, N., Ashajyothi, M., Patel, A., & Fekam, F. B. (2019). Endophytic bacteria of desert cactus (Euphorbia trigonas Mill) confer drought tolerance and induce growth promotion in tomato (Solanum lycopersicum L.). Microbiological Research, 228(1), 1-15. doi: 10.1016/j.micres.2019.126302 DOI: https://doi.org/10.1016/j.micres.2019.126302
Elhaissoufi, W., Khourchi, S., Ibnyasser, A., Ghoulam, C., Rchiad, Z., Zeroual, Y., Lyamlouli, K., & Bargaz, A. (2020). Phosphate solubilizing rhizobacteria could have a stronger influence on wheat root traits and aboveground physiology than rhizosphere P solubilization. Frontiers in Plant Science, 11(1), 1-15. doi: 10.3389/fpls.2020.00979 DOI: https://doi.org/10.3389/fpls.2020.00979
Estrada, G. A., Baldani, V. L. D., Oliveira, D. M., Urquiaga, S., & Baldani, J. I. (2013). Selection of phosphate-solubilizing diazotrophic Herbaspirillum and Burkholderia strains and their effect on rice crop yield and nutrient uptake. Plant and Soil, 369(1), 115-129. doi: 10.1007/s11104-012-1550-7 DOI: https://doi.org/10.1007/s11104-012-1550-7
Fankem, H., Mafokoua, H. L., Nkot, L. N., Simo, C., Dondjou, D. T., Tchakounte, G. V. T., Nwaga, D., & Etoa, F. X. (2016). Biodiversity of the phosphate solubilizing microorganisms (PSMs) population from the rice rhizosphere soils of the two agroecological zones of Cameroon. International Journal of Biological and Chemical Sciences, 9(5), 2284-2299. doi: 10.4314/ijbcs.v9i5.3 DOI: https://doi.org/10.4314/ijbcs.v9i5.3
Fankem, H., Nwaga, D., Deubel, A., Dieng, L., Merbach, W., & Etoa, F. X. (2006). Occurrence and functioning of phosphate solubilizing microorganisms from oil palm tree (Elaeis guineensis) rhizosphere in Cameroon. African Journal of Biotechnology, 5(24), 2450-2460. doi: 10.5897/AJB06.324
Ferreira, D. F. (2019). Sisvar. A computer analysis system to fixed effects split plot type designs. Revista Brasileira de Biometria, 37(4), 529-535. doi: 10.28951/rbb.v37i4.450 DOI: https://doi.org/10.28951/rbb.v37i4.450
Food and Agriculture Organization of the United States (2023). Rankings: countries by commodity. https://www.fao.org/faostat/en/#rankings/countries_by_commodity
Hara, F. A. S., & Oliveira, L. A. (2004). Características fisiológicas e ecológicas de isolados de rizóbios oriundos de solos ácidos e álicos de Presidente Figueiredo, Amazonas. Acta Amazônica, 34(3), 343-357. doi: 10.1590/S0044-59672004000300002 DOI: https://doi.org/10.1590/S0044-59672004000300002
Irfan, M., Aziz, T., Maqsood, M. A., Bilal, H. M., Siddique, K. H. M., & Xu, M. (2020). Phosphorus (P) use efficiency in rice is linked to tissue-specific biomass and P allocation patterns. Scientific Reports, 10(1), 1-14. doi: https://doi.org/10.1038/s41598-020-61147-3 DOI: https://doi.org/10.1038/s41598-020-61147-3
Khan, M. U., Sessitsch, A., Harris, M., Fatima, K., Imran, A., Arslan, M., Shabir, G., Khan, Q. M., & Afzal, M. (2015). Cr-resistant rhizo- and endophytic bacteria associated with Prosopis juliflora and their potential as phytoremediation enhancing agents in metal-degraded soils. Frontiers in Plant Science, 5(755), 1-10. doi: 10.3389/fpls.2014.00755 DOI: https://doi.org/10.3389/fpls.2014.00755
Kour, D., Rana, K. L., Kaur, T., Yadav, N., Yadav, A. N., Kumar, M., Kumar, V., Dhaliwal, H. S., & Saxena, A. K. (2021). Biodiversity, current developments, and potential biotechnological applications of phosphorus-solubilizing and -mobilizing microbes: a review. Pedosphere, 31(1), 43-75. doi: 10.1016/S1002-0160(20)60057-1 DOI: https://doi.org/10.1016/S1002-0160(20)60057-1
Li, X., Peng, D., Zhang, Y., Ju, D., & Guan, C. (2020). Klebsiella sp. PD3, a phenanthrene (PHE) - degrading strain with plant growth promoting properties enhances the PHE degradation and stress tolerance in rice plants. Ecotoxicology and Environmental Safety, 201(1), 1-15. doi: 10.1016/j.ecoenv.2020.110804 DOI: https://doi.org/10.1016/j.ecoenv.2020.110804
Lin, L., Li, Z., Hu, C., Zhang, X., Chang, S., Yang, L., Li, Y., & An, Q. (2012). Plant growth-promoting nitrogen-fixing Enterobacteria are in association with sugarcane plants growing in Guangxi, China. Microbes and Environments, 27(4), 391-398. doi: 10.1264/jsme2.me11275 DOI: https://doi.org/10.1264/jsme2.ME11275
Maguire, J. D. (1962). Speed of germination aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2(2), 176-177. doi: 10.2135/cropsci1962.0011183X000200020033x DOI: https://doi.org/10.2135/cropsci1962.0011183X000200020033x
Ministério da Agricultura Pecuária e Abastecimento (2009). Regras para análise de sementes. https://www.gov.br/agricultura/pt-br/assuntos/insumos-agropecuarios/arquivos-publicacoes-insumos/2946_regras_analise__sementes.pdf
Nakagawa, J. (2020). Testes de vigor baseados na avaliação das plântulas. In F. C. Krzyzanowski, R. D. Vieira, J. Marcos Fº., & J. B. França Neto (Eds.), Vigor de sementes: conceitos e testes (2a ed., Cap. 2, pp. 1-21). Londrina.
Panda, B., Rahman, H., & Panda, J. (2016). Phosphate solubilizing bacteria from the acidic soils of Eastern Himalayan region and their antagonistic effect on fungal pathogens. Rhizosphere, 2(1), 62-71. doi: 10.1016/j.rhisph.2016.08.001 DOI: https://doi.org/10.1016/j.rhisph.2016.08.001
Ribeiro, V. P., Almeida, C. N. S., Mattos, B. B., Oliveira, C. A., Gomes, E. A., & Marriel, I. E. (2014). Potencial de solubilização de fosfato de ferro in vitro e produção de sideróforos por microrganismos endofíticos da cultura de milho. Anais da Sociedade Brasileira de Ciência do Solo, FertBio, Araxá, MG, Brasil, 2014.
Roslan, M. A. M., Zulkifli, N. N., Sobri, Z. M., Zuan, A. T. K., Cheak, S. C., & Rahman, N. A. A. (2020). Seed biopriming with P- and K-solubilizing Enterobacter hormaechei sp. improves the early vegetative growth and the P and K uptake of okra (Abelmoschus esculentus) seedling. Plos One, 15(7), 1-41. doi: 10.1371/journal.pone.0232860 DOI: https://doi.org/10.1371/journal.pone.0232860
Santos, A. A., Agustini, J. A., Maltoni, K. L., & Cassiolato, A. M. R. (2018). Addition of waste and introduction of microorganisms after 45 years of soil degradation. Revista Ciência Agronômica, 49(3), 363-370. doi: 10.5935/1806-6690.20180041 DOI: https://doi.org/10.5935/1806-6690.20180041
Santos, H. O., Botelho, F. B. S., Fonseca, A. C. A., Uenojo, R. K., Mendes, M. P., & Pinho, E. V. R. V. (2015). Quality of seeds of upland rice lines adapted to the state of Minas Gerais, Brazil. American Journal of Plant Sciences, 6(12), 1920-1927. doi: 10.4236/ajps.2015.612193 DOI: https://doi.org/10.4236/ajps.2015.612193
Shahzad, S. M., Arif, M. S., Riaz, M., Ashraf, M., Yasmeen, T., Zaheer, A., Bragazza, L., Buttler, A., & Robroek, B. J. M. (2017). Interaction of compost additives with phosphate solubilizing rhizobacteria improved maize production and soil biochemical properties under dryland agriculture. Soil and Tillage Research, 174(1), 70-80. doi: 10.1016/j.still.2017.06.004 DOI: https://doi.org/10.1016/j.still.2017.06.004
Silva, G. N., F., & Vidor, C. (2000). Solubilização de fostatos por microrganismos na presença de fontes de carbono. Revista Brasileira de Ciência do Solo, 24(2), 311-319. doi: 10.1590/S0100-06832000000200008 DOI: https://doi.org/10.1590/S0100-06832000000200008
Solanki, M. K., Wang, Z., Wang, F. Y., Li, C. N., Gupta, C. L., Singh, R. K., Malviya, M. K., Singh, P., Yang, L. T., & Li, Y. R. (2020). Assessment of diazotrophic proteobacteria in sugarcane rhizosphere when intercropped with legumes (peanut and soybean) in the field. Frontiers in Microbiology, 11(1), 1-12. doi: 10.3389/fmicb.2020.01814 DOI: https://doi.org/10.3389/fmicb.2020.01814
Souchie, E. L., & Abboud, A. C. S. (2007). Solubilização de fosfato por microrganismos rizosféricos de genótipos de Guandu cultivados em diferentes classes de solo. Semina: Ciências Agrárias, 28(1), 11-18. doi: 10.5433/1679-0359.2007v28n1p11 DOI: https://doi.org/10.5433/1679-0359.2007v28n1p11
Sylvester-Bradley, R., Asakawa, N., La Torraca, S., Magalhães, F. M. M., Oliveira, L. A., & Pereira, R. M. (1982). Levantamento quantitativo de microrganismos solubilizadores de fosfatos na rizosfera de gramíneas e leguminosas forrageiras na Amazônia. Acta Amazônica, 12(1), 15-22. doi: 10.1590/1809-43921982121015 DOI: https://doi.org/10.1590/1809-43921982121015
Tan, B. L., & Norhaizan, M. E. (2020). Rice by-products: phytochemicals and food products application. Springer. DOI: https://doi.org/10.1007/978-3-030-46153-9
Timofeeva, A., Galyamova, M., & Sedykh, S. (2022). Prospects for using phosphate-solubilizing microorganisms as natural fertilizers in agriculture. Plants, 11(16), 1-23. doi: 10.3390/plants11162119 DOI: https://doi.org/10.3390/plants11162119
Tunes, L. M., Tavares, L. C., & Barros, A. C. S. A. (2012). Envelhecimento acelerado como teste de vigor para sementes de arroz. Revista de Ciências Agrárias, 35(1), 120-127. doi: 10.19084/rca.16072
Withers, P. J. A., Rodrigues, M., Soltangheisi, A., Carvalho, T. S. de, Guilherme, L. R. G., Benites, V. de M., Gatiboni, L. C., Sousa, D. M. G. de, Nunes, R. de S., Rosolem, C. A., Andreote, F. D., Oliveira, A. de, Coutinho, E. L. M., & Pavinato, P. S. (2018). Transitions to sustainable management of phosphorus in Brazilian agriculture. Scientific Reports, 8(1), 1-13. doi: 10.1038/s41598-018-20887-z DOI: https://doi.org/10.1038/s41598-018-20887-z
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