Most common pathogens from cows with subclinical mastitis in northwestern Paraná, Southern Brazil and their antimicrobial susceptibility
DOI:
https://doi.org/10.5433/1679-0359.2022v43n5p2385Keywords:
Antibiotic resistance, Staphylococcus, Streptococcus.Abstract
Considering the high prevalence of subclinical mastitis and its impacts on milk production, thematic studies are needed and can provide strategic data for its control. This study aimed at investigating the most frequent microorganisms associated with subclinical mastitis in dairy cows in the northwest State of Paraná, Brazil and its sensitivity to antibiotics. A total of 478 foremilk samples were tested. Streptococci were the most frequently genus observed (63%), followed by staphylococci (31%). Streptococcus mutans, S. sanguis, and S. uberis were the most frequently identified species. For Streptococcus spp., the antimicrobial resistance was higher for Ceftiofur, whereas for Staphylococcus spp. it was also possible to verify a high occurrence of resistance for Gentamicin + Amoxicillin. Our results strengthen the knowledge of the microbiological agent and antibiotic-resistance patterns of pathogens at regional level, targeting future strategies to reduce the environmental spread of pathogenic strains resistant to antibiotics.Downloads
Download data is not yet available.
References
Abril, A. G., Carrera, M., Bohme, K., Barros-Velázquez, J., Rama, J. L. R., Calo-Mata, P., & Villa, T. G. (2020). Proteomic characterization of antibiotic resistance, and production of antimicrobial and virulence factors in streptococcus species associated with bovine mastitis. Could enzybiotics represent novel therapeutic agents against these pathogens? Antibiotics, 9(6), 1-24. doi: 10.3390/antibiotics9060302
Bergey, D. H. (1994). Bergey's manual of determinative bacteriology. Lippincott Williams & Wilkins.
Boireau, C., Cazeau, G., Jarrige, N., Calavas, D., Madec, J. Y., Leblond, A., & Gay, É. (2018). Antimicrobial resistance in bacteria isolated from mastitis in dairy cattle in France, 2006-2016. Journal of Dairy Science, 101(10), 9451-9462. doi: 10.3168/jds.2018-14835
Clinical and Laboratory Standards Institute. (2018). Performance standards for antimicrobial susceptibility testing (13th ed.) Wayne, PA: Clinical and Laboratory Standards Institute.
Costa, F. N., Belo, N. O., Costa, E. A., Andrade, G. I., Pereira, L. S., Carvalho, I. A., & Santos, R. L. (2018). Frequency of enterotoxins, toxic shock syndrome toxin-1, and biofilm formation genes in Staphylococcus aureus isolates from cows with mastitis in the Northeast of Brazil. Tropical Animal Health and Production, 50(5), 1089-1097. doi: 10.1007/s11250-018-1534-6
Funke, G., & Bernard, K. A. (2015). Coryneform Gram‐positive rods. In J. H. Jorgensen, K. C. Carroll, G. Funke, M. A. Pfaller, M. L. Landry, S. S. Richter, & D. W. Warnock (Eds.), Manual of Clinical Microbiology (11th ed. Cap. 28, pp. 474-503). doi: 10.1128/9781555817381.ch28
Gomez, D. L. S., Lozano, A. S., Alanis, H. F. M., Capetillo-Hernandez, G. R., Capetillo, E. G. T., Cepeda, S. E. N., & Solis-Soto, J. M. (2021). Streptococcus: an orthodontic point of view. International Journal of Applied Dental Sciences, 7(3), 237-241. doi: 10.22271/oral.2021.v7.i3d.1306
Kateete, D. P., Kimani, C. N., Katabazi, F. A., Okeng, A., Okee, M. S., Nanteza, A., & Najjuka, F. C. (2010). Identification of Staphylococcus aureus: DNase and Mannitol salt agar improve the efficiency of the tube coagulase test. Annals of Clinical Microbiology and Antimicrobials, 9(1), 1-7. doi: 10.1186/1476-0711-9-23
Oliver, J. P., Gooch, C. A., Lansing, S., Schueler, J., Hurst, J. J., Sassoubre, L., & Aga, D. S. (2020). Invited review: fate of antibiotic residues, antibiotic-resistant bacteria, and antibiotic resistance genes in US dairy manure management systems. Journal of Dairy Science, 103(2), 1051-1071. doi: 10.3168/jds.2019-16778
Oliver, S. P. (2004). Microbiological procedures for the diagnosis of bovine udder infection and determination of milk quality. National Mastitis Council.
Rousham, E. K., Unicomb, L., & Islam, M. A. (2018). Human, animal and environmental contributors to antibiotic resistance in low-resource settings: Integrating behavioural, epidemiological and one health approaches. Proceedings of the Royal Society B: Biological Sciences, 285(1876), 1-9. doi: 10.1098/rspb. 2018.0332
Ruegg, P. L. (2020). Understanding the economic impact of mastitis therapy - the role of duration and drug selection. American Association of Bovine Practitioners Proceedings, 53(1), 84-91. doi: 10.21423/ aabppro20207976
Scherpenzeel, C. G. M., Hogeveen, H., Maas, L., & Lam, T. J. G. M. (2018). Economic optimization of selective dry cow treatment. Journal of Dairy Science, 101(2), 1530-1539. doi: 10.3168/jds.2017-13076
Sharma, C., Rokana, N., Chandra, M., Singh, B. P., Gulhane, R. D., Gill, J. P. S., & Panwar, H. (2018). Antimicrobial resistance: its surveillance, impact, and alternative management strategies in dairy animals. Frontiers in Veterinary Science, 4(2), 1-12. doi: 10.3389/fvets.2017.00237
Tomazi, T., Ferreira, G. C., Orsi, A. M., Gonçalves, J. L., Ospina, P. A., Nydam, D. V., & Santos, M. V. dos. (2018). Association of herd-level risk factors and incidence rate of clinical mastitis in 20 Brazilian dairy herds. Preventive Veterinary Medicine, 161(1), 9-18. doi: 10.1016/j.prevetmed.2018.10.007
Valmorbida, M. K., Santos Carneiro, D. C. dos, Prior, K. C., Griebeler, E., Troncarelli, M. Z., & Dezen, D. (2017). Etiology and in vitro antimicrobial susceptibility profile of strains isolated from bovine mastitis in dairy herds from the midwest region of Santa Catarina state, Brazil. Acta Veterinaria Brasilica, 11(4), 219-225. doi: 10.21708/avb.2017.11.4.7266
Winn, J. W., Allen, S., Janda, W., Koneman, E., Procop, G., Schreckenberger, P., & Woods, G. (2012). Diagnóstico microbiológico: texto e atlas colorido. Guanabara Koogan.
Bergey, D. H. (1994). Bergey's manual of determinative bacteriology. Lippincott Williams & Wilkins.
Boireau, C., Cazeau, G., Jarrige, N., Calavas, D., Madec, J. Y., Leblond, A., & Gay, É. (2018). Antimicrobial resistance in bacteria isolated from mastitis in dairy cattle in France, 2006-2016. Journal of Dairy Science, 101(10), 9451-9462. doi: 10.3168/jds.2018-14835
Clinical and Laboratory Standards Institute. (2018). Performance standards for antimicrobial susceptibility testing (13th ed.) Wayne, PA: Clinical and Laboratory Standards Institute.
Costa, F. N., Belo, N. O., Costa, E. A., Andrade, G. I., Pereira, L. S., Carvalho, I. A., & Santos, R. L. (2018). Frequency of enterotoxins, toxic shock syndrome toxin-1, and biofilm formation genes in Staphylococcus aureus isolates from cows with mastitis in the Northeast of Brazil. Tropical Animal Health and Production, 50(5), 1089-1097. doi: 10.1007/s11250-018-1534-6
Funke, G., & Bernard, K. A. (2015). Coryneform Gram‐positive rods. In J. H. Jorgensen, K. C. Carroll, G. Funke, M. A. Pfaller, M. L. Landry, S. S. Richter, & D. W. Warnock (Eds.), Manual of Clinical Microbiology (11th ed. Cap. 28, pp. 474-503). doi: 10.1128/9781555817381.ch28
Gomez, D. L. S., Lozano, A. S., Alanis, H. F. M., Capetillo-Hernandez, G. R., Capetillo, E. G. T., Cepeda, S. E. N., & Solis-Soto, J. M. (2021). Streptococcus: an orthodontic point of view. International Journal of Applied Dental Sciences, 7(3), 237-241. doi: 10.22271/oral.2021.v7.i3d.1306
Kateete, D. P., Kimani, C. N., Katabazi, F. A., Okeng, A., Okee, M. S., Nanteza, A., & Najjuka, F. C. (2010). Identification of Staphylococcus aureus: DNase and Mannitol salt agar improve the efficiency of the tube coagulase test. Annals of Clinical Microbiology and Antimicrobials, 9(1), 1-7. doi: 10.1186/1476-0711-9-23
Oliver, J. P., Gooch, C. A., Lansing, S., Schueler, J., Hurst, J. J., Sassoubre, L., & Aga, D. S. (2020). Invited review: fate of antibiotic residues, antibiotic-resistant bacteria, and antibiotic resistance genes in US dairy manure management systems. Journal of Dairy Science, 103(2), 1051-1071. doi: 10.3168/jds.2019-16778
Oliver, S. P. (2004). Microbiological procedures for the diagnosis of bovine udder infection and determination of milk quality. National Mastitis Council.
Rousham, E. K., Unicomb, L., & Islam, M. A. (2018). Human, animal and environmental contributors to antibiotic resistance in low-resource settings: Integrating behavioural, epidemiological and one health approaches. Proceedings of the Royal Society B: Biological Sciences, 285(1876), 1-9. doi: 10.1098/rspb. 2018.0332
Ruegg, P. L. (2020). Understanding the economic impact of mastitis therapy - the role of duration and drug selection. American Association of Bovine Practitioners Proceedings, 53(1), 84-91. doi: 10.21423/ aabppro20207976
Scherpenzeel, C. G. M., Hogeveen, H., Maas, L., & Lam, T. J. G. M. (2018). Economic optimization of selective dry cow treatment. Journal of Dairy Science, 101(2), 1530-1539. doi: 10.3168/jds.2017-13076
Sharma, C., Rokana, N., Chandra, M., Singh, B. P., Gulhane, R. D., Gill, J. P. S., & Panwar, H. (2018). Antimicrobial resistance: its surveillance, impact, and alternative management strategies in dairy animals. Frontiers in Veterinary Science, 4(2), 1-12. doi: 10.3389/fvets.2017.00237
Tomazi, T., Ferreira, G. C., Orsi, A. M., Gonçalves, J. L., Ospina, P. A., Nydam, D. V., & Santos, M. V. dos. (2018). Association of herd-level risk factors and incidence rate of clinical mastitis in 20 Brazilian dairy herds. Preventive Veterinary Medicine, 161(1), 9-18. doi: 10.1016/j.prevetmed.2018.10.007
Valmorbida, M. K., Santos Carneiro, D. C. dos, Prior, K. C., Griebeler, E., Troncarelli, M. Z., & Dezen, D. (2017). Etiology and in vitro antimicrobial susceptibility profile of strains isolated from bovine mastitis in dairy herds from the midwest region of Santa Catarina state, Brazil. Acta Veterinaria Brasilica, 11(4), 219-225. doi: 10.21708/avb.2017.11.4.7266
Winn, J. W., Allen, S., Janda, W., Koneman, E., Procop, G., Schreckenberger, P., & Woods, G. (2012). Diagnóstico microbiológico: texto e atlas colorido. Guanabara Koogan.
Downloads
Published
2022-11-17
How to Cite
Eleodoro, J. I., Headley, S. A., Zanoni, A. P. K., Giordano, L. G. P., & Fagnani, R. (2022). Most common pathogens from cows with subclinical mastitis in northwestern Paraná, Southern Brazil and their antimicrobial susceptibility. Semina: Ciências Agrárias, 43(5), 2385–2394. https://doi.org/10.5433/1679-0359.2022v43n5p2385
Issue
Section
Communication
License
Copyright (c) 2022 Semina: Ciências Agrárias
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Semina: Ciências Agrárias adopts the CC-BY-NC license for its publications, the copyright being held by the author, in cases of republication we recommend that authors indicate first publication in this journal.
This license allows you to copy and redistribute the material in any medium or format, remix, transform and develop the material, as long as it is not for commercial purposes. And due credit must be given to the creator.
The opinions expressed by the authors of the articles are their sole responsibility.
The magazine reserves the right to make normative, orthographic and grammatical changes to the originals in order to maintain the cultured standard of the language and the credibility of the vehicle. However, it will respect the writing style of the authors. Changes, corrections or suggestions of a conceptual nature will be sent to the authors when necessary.
