Multidrug resistance in Shiga toxin-producing Escherichia coli (STEC) isolated from broiler chickens at slaughter
DOI:
https://doi.org/10.5433/1679-0359.2021v42n6SUPL2p3813Keywords:
Broiler carcasses, Escherichia coli, Multidrug-resistance, STEC.Abstract
Broiler chickens and derived products are a key source of Shiga toxin-producing Escherichia coli (STEC) in humans. This pathotype is responsible for causing severe episodes of diarrhea, which can progress to systemic complications. A rapid and accurate diagnosis of the disease, and early treatment of the infection with antimicrobials, can prevent it worsening. However, multidrug-resistant strains have potentially negative implications for treatment success. In this context, the aim of the present study was to isolate and identify multidrug-resistant STEC strains from broiler chickens and carcasses. Of 171 E. coli strains, isolated by conventional microbiological techniques and submitted to Polymerase Chain Reaction (PCR), for detection of stx1 and stx2 genes, 21.05% (36/171) were STEC pathotype, and most of them (66.67% - 24/36) carried both stx1 and eae genes. The multidrug resistance pattern was observed in 75% (27/36) of STEC strains. The presence of STEC in broiler chickens and carcasses reinforces that these sources may act as reservoirs for this pathotype. Multidrug-resistant bacteria contaminating animal products represent a public health issue because of the possibility of spread of multidrug-resistant determinants in the food chain and a higher risk of failure in human treatment when antimicrobials are needed.Downloads
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References
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Alonso, M. Z., Lucchesi, P. M. A., Rodríguez, E. M., Parma, A. E., & Padola, N. L. (2012). Enteropathogenic ( EPEC ) and shigatoxigenic Escherichia coli (STEC) in broiler chickens and derived products at different retail stores. Food Control, 23(2), 351-355. doi: 10.1016/j.foodcont.2011.07.030
Andreatti, R. L., Fº., Gonçalves, G. A. M., Okamoto, A. S., & Lima, E. T. de. (2011). Comparação de métodos para extração de DNA na reação em cadeia da polimerase para detecção de Salmonella enterica sorovar Enteritidis em produtos avícolas. Ciência Animal Brasileira, 12(1), 115-119. doi: 10.5216/cab. v12i1.3774
Barros, M. R., Silveira, W. D. da, Araújo, J. M. de, Costa, E. P., Oliveira, A. A. da F., Santos, A. P. da S. F.,... Mota, R. A. (2012). Resistência antimicrobiana e perfil plasmidial de Escherichia coli isolada de frangos de corte e poedeiras comerciais no Estado de Pernambuco. Pesquisa Veterinária Brasileira, 32(5), 405-410. doi: 10.1590/S0100-736X2012000500008
Blaak, H., Hoek, A. H. A. M. Van, Hamidjaja, R. A., Plaats, R. Q. J. Van Der, Heer, L. K., Maria, A.,... Schets, F. M. (2015). Distribution , numbers , and diversity of ESBL- producing E . coli in the poultry farm environment. Plos One, 8(13), 1-23. doi: 10.1371/journal.pone.0135402
Brasil, M. da S. (2019). Surtos de doenças transmitidas por alimentos no Brasil. Brasília: Secretaria de Vigilância em Saúde. Retrieved from https://portalarquivos2.saude.gov.br/images/pdf/2019/fevereiro /15/Apresenta----o-Surtos-DTA---Fevereiro-2019.pdf
Clinical and Laboratory Standards Institute (2018). Performance standards for antimicrobial susceptibility testing, M100 performance standards for antimicrobial susceptibility testing (28nd ed.). Wayne: CLSI.
Doregiraee, F., Alebouyeh, M., Fasaei, B. N., Charkhkar, S., Tajedin, E., & Zali, M. R. (2016). Isolation of atypical enteropathogenic and shiga toxin encoding Escherichia coli strains from poultry in Tehran, Iran. Gastroenterology and Hepatology from Bed to Bench, 9(1), 53-57. doi: 10.22037/ghfbb.v1i9.866
El-Rami, F. E., Rahal, E. A., Sleiman, F. T., & Abdelnoor, A. M. (2012). Identification of virulence genes among antibacterial-resistant Escherichia coli isolated from poultry. Advanced Studies in Biology, 4(8), 385-396. Retrived from http://www.m-hikari.com/asb/asb2012/asb5-8-2012/abdelnoorASB5-8-2012. pdf
Ethelberg, S., Olsen, K. E. P., Scheutz, F., Jensen, C., Schiellerup, P., Engberg, J.,... Mølbak, K. (2004). Virulence factors for virulence factors for hemolytic uremic syndrome, Denmark. Emerging Infectious Diseases, 10(5), 843-847. doi: 10.3201/eid1005.030576
Fürst, S., Scheef, J., Bielaszewska, M., Rüssmann, H., Schmidt, H., & Karch, H. (2000). Identification and characterisation of Escherichia coli strains of O157 and non-O157 serogroups containing three distinct Shiga toxin genes. Journal of Medical Microbiology, 49(4), 383-386. doi: 10.1099/0022-1317-49-4-383
Geerdes-Fenge, H. F., Lobermann, M., Nrnberg, M., Fritzsche, C., Koball, S., Henschel, J.,... Reisinger, E. C. (2013). Ciprofloxacin reduces the risk of hemolytic uremic syndrome in patients with Escherichia coli O104 : H4-associated diarrhea. Infection, 2013(41), 669-673. doi: 10.1007/s15010-012-0387-6
Gobius, K. S., Higgs, G. M., & Desmarchelier, P. M. (2003). Presence of activatable shiga toxin genotype (stx 2d) in shiga toxigenic Escherichia coli from livestock sources. Journal of Clinical Microbiology, 41(8), 3777-3783. doi: 10.1128/JCM.41.8.3777
Granados-Chinchilla, F., & Rodríguez, C. (2017). Tetracyclines in food and feedingstuffs: from regulation to analytical methods, bacterial resistance, and environmental and health implications. Journal of Analytical Methods in Chemistry, 2017(1), 1-24. doi: 10.1155/2017/1315497
Hannah, G., Bopp, C., Strockbine, N., Atkinson, R., Baselski, V., Body, B.,... Gerner-Smidt, P. (2009). Recommendations for diagnosis of shiga toxin-producing Escherichia coli infections by clinical laboratories. Centers for Disease Control and Prevention, 58(RR12);1-14. Retrieved from https://www. cdc.gov/mmwr/preview/mmwrhtml/rr5812a1.htm
Jafari, F., Hamidian, M., Rezadehbashi, M., Doyle, M., Salmanzadeh-ahrabi, S., Derakhshan, F., & Zali, M. R. (2009). Prevalence and antimicrobial resistance of diarrheagenic Escherichia coli and Shigella species associated with acute diarrhea in Tehran, Iran. Canadian Journal of Infectious Diseases and Medical Microbiology, 20(3), e56-e62. doi: 10.1155/2009/341275
Krumperman, P. H. (1983). Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of faecal contamination of water. Environmental Science and Pollution Research, 22(14), 10969-10980. doi: 10.1007/s11356-014-3887-3
MacFaddin, J. F. (2000). Biochemical tests for identification of medical bacteria (3nd ed.). Baltimore (Md.): Williams and Wilkins.
Magiorakos, A., Srinivasan, A., Carey, R. B., Carmeli, Y., Falagas, M. E., Giske, C. G.,... Hindler, J. F. (2011). bacteria : an international expert proposal for interim standard definitions for acquired resistance. Clinical Microbiology and Infection, 18(3), 268-281. doi: 10.1111/j.1469-0691.2011.03570.x
Matussek, A., Jernberg, C., Einemo, I., Monecke, S., & Ehricht, R. (2017). Genetic makeup of Shiga toxin-producing Escherichia coli in relation to clinical symptoms and duration of shedding: a microarray analysis of isolates from Swedish children. European Journal of Clinical Microbiology and Infectious Diseases, 2017(36), 1433-1441. doi: 10.1007/s10096-017-2950-7
Momtaz, H., & Jamshidi, A. (2011). Shiga toxin-producing Escherichia coli isolated from chicken meat in Iran: serogroups, virulence factors , and antimicrobial resistance properties. Poultry Science, 92(5), 1305-1313. doi: 10.3382/ps.2012-02542
Osundiya, O., Oladele, R., & Oduyebo, O. (2013). Multiple antibiotic resistance (MAR) indices of Pseudomonas and Klebsiella species isolates in Lagos University Teaching Hospital. African Journal of Clinical and Experimental Microbiology, 14(3), 164-168. doi: 10.4314/ajcem.v14i3.8
Paton, A. W., & Paton, J. C. (1998). Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157. Journal of Clinical Microbiology, 36(2), 598-602. doi: 10.1128/JCM.36.2.598-602.1998
Pèrez-Cruz, F. G., Villa-Díaz, P., Pintado-Delgado, M. C., Fernández Rodriguez, M. L., Blasco-Martínez, A., & Pérez-Fernández, M. (2017). Hemolytic uremic syndrome in adults: a case report Fabiel. World Journal of Critical Care Medicine, 3141(2), 135-139. doi: 10.5492/wjccm.v6.i2.135
Persson, S., Olsen, K. E. P., Ethelberg, S., & Scheutz, F. (2007). Subtyping method for Escherichia coli Shiga toxin (Verocytotoxin) 2 variants and correlations to clinical manifestations. Journal of Clinical Microbiology, 45(6), 2020-2024. doi: 10.1128/JCM.02591-06
Ranjbar, R., Masoudimanesh, M., Dehkordi, F. S., & Jonaidi-Jafari, N. (2017). Shiga (Vero) - toxin producing Escherichia coli isolated from the hospital foods; virulence factors, o-serogroups and antimicrobial resistance properties. Antimicrobial Resistance & Infection Control, 2017(6:4), 1-11. doi: 10.1186/s13756-016-0163-y
Runa, J. A., Lijon, M. B., & Rahman, M. A. (2018). Detection of multidrug resistant and shiga toxin producing Escherichia coli (STEC ) from apparently healthy broilers in Jessore, Bangladesh. Frontiers in Environmental Microbiology, 4(1), 16-21. doi: 10.11648/j.fem.20180401.13
Verraes, C., Van Boxstael, S., Van Meervenne, E., Van Coillie, E., Butaye, P., Catry, B.,... Herman, L. (2013). Antimicrobial resistance in the food chain: a review. International Journal of Environmental Research and Public Health, 10(7), 2643-2669. doi: 10.3390/ijerph10072643
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2021-10-08
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Ornellas, R. P., Lopes, H. P., Baptista, D. de Q., Dias, T. S., Figueira, A. de A., Costa, G. A., … Abreu, D. L. da C. (2021). Multidrug resistance in Shiga toxin-producing Escherichia coli (STEC) isolated from broiler chickens at slaughter. Semina: Ciências Agrárias, 42(6SUPL2), 3813–3824. https://doi.org/10.5433/1679-0359.2021v42n6SUPL2p3813
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