Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation

Authors

  • José Geraldo Zaparolli Vieira Universidade Estadual de Londrina
  • Jorge Kaoro Yamakami Universidade Estadual de Londrina
  • Ricardo Sfeir de Aguiar Universidade Estadual de Londrina
  • Lilian Keiko Unemoto Universidade Estadual de Londrina
  • Ricardo Tadeu de Faria Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0359.2007v28n2p207

Keywords:

Nitrogen, orchid, tissue culture

Abstract

The nitrogen is essentially important for most in vitro cultivated species. It is regarded to the physiological and biochemical processes involved in growth control, differentiation and morphogenesis, and casein is one of the main nitrogen supplementation sources. The objective of this work was to evaluate the influence of different concentrations of hydrolized casein on the in vitro vegetative growth and rooting of Oncidium baueri (Orchidaceae). The treatments tested were: T1- 0.0 g.L-1; T2- 0.5 g.L-1; T3- 1.0 g. L-1; T4 –2.0 g.L-1; T5- 4.0 g.L-1 of hydrolized casein. The culture medium used was the Murashige and Skoog modified with half macronutrients concentration. Total fresh mass, aerial part height, biggest root length, roots number and lateral sprouts number were evaluated after six months from the beginning of the experiment. It was not detected any beneficial effects of casein on vegetative and root development of Oncidium baueri. The percentage of surviving plants was 100%, 95 % and 94%, respectively for T1, T2 and T3, and 80% of surviving plants for T4 and T5.

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Author Biographies

José Geraldo Zaparolli Vieira, Universidade Estadual de Londrina

Engº Agrº. Mestre em Agronomia, Universidade Estadual de Londrina, PR

Jorge Kaoro Yamakami, Universidade Estadual de Londrina

Engº Agrº. Mestre em Agronomia, Universidade Estadual de Londrina, PR

Ricardo Sfeir de Aguiar, Universidade Estadual de Londrina

Engº Agrº. Mestre em Agronomia, Universidade Estadual de Londrina, PR

Lilian Keiko Unemoto, Universidade Estadual de Londrina

Bióloga. Mestre em Fitotecnia, Universidade Estadual de Londrina, PR.

 

Ricardo Tadeu de Faria, Universidade Estadual de Londrina

Eng.º Agrº. Prof. Adjunto. Depto. Agronomia. UEL, Londrina. PR.

Published

2007-10-30

How to Cite

Vieira, J. G. Z., Yamakami, J. K., Aguiar, R. S. de, Unemoto, L. K., & Faria, R. T. de. (2007). Influence of hydrolized casein on the Oncidium baueri (Orchidaceae) in vitro cultivation. Semina: Ciências Agrárias, 28(2), 207–212. https://doi.org/10.5433/1679-0359.2007v28n2p207

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