Selection of Dendrobium phalaenopsis (Orchidaceae) genotypes in the in vitro propagation and acclimatization phases

Authors

  • Alessandro Borini Lone Universidade Estadual de Londrina
  • Cristiane Muniz Barbosa Universidade Estadual de Londrina
  • Ricardo Tadeu de Faria Universidade Estadual de Londrina
  • Lúcia Sadayo Assari Takahashi Universidade Estadual de Londrina
  • Inês Cristina de Batista Fonseca Universidade Estadual de Londrina

DOI:

https://doi.org/10.5433/1679-0359.2008v29n4p755

Keywords:

Self-fertilizations, Artifcial pollination, Survival tax.

Abstract

The hybridization of orchids have been done to propitiate a production of cultivars with commercial interesting that show high vigor, great number of fowers and variability of colors. This research had the objective to select favorable genotypes of Dendrobium phalaenopsis for in vitro propagation and acclimatization, resulted from crossing and self- fertilizations of selected plants matrix. For the realization of crossing and self-fertilizations 56 plants of D. phalaenopsis were utilized, totalizing 109 combinations. From the 109 crossing and self-pollinations, only 13 crossing and 3 self-pollinations showed enough number of seedlings for an evaluation. After fve months, 15 seedlings from each crossing and self-pollinationss were evaluated by the following characteristics: length of the aerial part, number of roots, weight of the total fresh mass, length of the biggest root and number of buds. The others seedlings were planted in trays of styrofoam and after four months the rate of survival were evaluated. The most favorable genotype for the in vitro propagation and acclimatization was the crossing DF18 x DF13.

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Author Biographies

Alessandro Borini Lone, Universidade Estadual de Londrina

Acadêmico de Biologia da UEL, Bolsista CNPq.

Cristiane Muniz Barbosa, Universidade Estadual de Londrina

Acadêmica de Biologia da UEL.

Ricardo Tadeu de Faria, Universidade Estadual de Londrina

Engenheiro Agrônomo, Doutor em Genética, Professor Associado do Departamento de Agronomia da UEL. Londrina, PR.

Lúcia Sadayo Assari Takahashi, Universidade Estadual de Londrina

Engenheira Agrônoma, Doutora em Agronomia, Professora Adjunta do Departamento de Agronomia da UEL. Londrina, PR.

Inês Cristina de Batista Fonseca, Universidade Estadual de Londrina

Engenheira Agrônoma, Doutora em Agronomia, Professora Adjunta do Departamento de Agronomia da UEL. Londrina, PR.

Published

2008-08-30

How to Cite

Lone, A. B., Barbosa, C. M., Faria, R. T. de, Takahashi, L. S. A., & Fonseca, I. C. de B. (2008). Selection of Dendrobium phalaenopsis (Orchidaceae) genotypes in the in vitro propagation and acclimatization phases. Semina: Ciências Agrárias, 29(4), 755–760. https://doi.org/10.5433/1679-0359.2008v29n4p755

Issue

Section

Articles