Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil
DOI:
https://doi.org/10.5433/1679-0359.2019v40n1p491Keywords:
Calves, Cryptosporidiosis, Molecular characterization, Nested-PCR.Abstract
The study was conducted on 25 properties of the settlements São José I and Salvador, located in the municipalities of Brejo Alegre and Birigui, in the State of São Paulo, Brazil. A record of variables was elaborated and included data such as gender, breed and age of the animals. A total of 231 stool samples were collected from bovines aged one to six months, 128 being females and 103 males, 131 crossbred and 100 Holstein. Among the 231 samples, 17 (7.36%) were positive for Cryptosporidium spp. both by malachite green negative staining and by nested-PCR. Of the 17 positive samples, 14 were sequenced in agarose gel. These sequences were detected between 99% and 100% of genetic similarity for the following species. One sequence was similar to C. parvum (AB513880.1), one to C. bovis (MF074602.1), two to C. ryanae (KT922233.1), one to C. felis (KM977642.1) and nine were similar for C. andersoni reference MF350628. C. andersoni was found in animals aged 2–6 months, an age group which is different from those described by several authors. The presence of C. parvum indicates that the calves in the studied region should be considered a potential source for zoonotic transmission. For the first time to our knowledge, C. felis was identified in cattle in America.Downloads
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References
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XIAO, L.; LIMOR, J.; MORGAN, U. M.; SULAIMAN, I. M.; THOMPSON, R. C. A.; LAL, A. A. Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites. Applied and Environmental Microbiology, Washington DC, v. 66, n. 12, p. 5499-5502, 2000.
CARDONA, G. A.; BEGOÑA BAILO, A. L.; CANO, L.; FUENTES, I.; CARMENA, D. Unexpected finding of feline-specific Giardia duodenalis assemblage F and Cryptosporidium felis in asymptomatic adult cattle in Northern Spain. Veterinary Parasitology, Amsterdam, v. 209, n. 3-4, p. 258-263, 2015.
DEL COCO, V. F.; CÓRDOBA, M. A.; BILBAO, G.; CASTRO, A. P. A.; BASUALDO, J. A.; FAYER, R.; SANTÍN, M. Cryptosporidium parvum GP60 subtypes in dairy cattle from Buenos Aires, Argentina. Research in Veterinary Science, v. 96, n. 2, p. 311-314, 2014.
DIXON, B.; PARRIGTON, L.; COOK, A.; PINTAR, K.; POLLARI, F.; KELTON, D.; FARBER, J. The potencialfor zoonotic transmission of Giardia duodenalis and Cryptosporidium sp. From beef and dairy cattle in Ontario, Canada. Veterinary Parasitology, Amsterdam, v. 175, n. 1-2, p. 20-26, 2011.
ELLIOT, A.; MORGAN, U. M.; THOMPSOM, R. C. A. Improved staining method for detecting Cryptosporidium oocysts in stools using malachite green. Journal of General and Applied Microbiology, Setagaya-ku, v. 45, n. 6, p. 139-142, 1999.
GONG, C.; CAO, X. F.; DENG, L.; LI, W.; HUANG, X.-M.; LAN, J.-C.; XIAO, Q. C.; ZHONG, Z. J.; FENG, F.; ZHANG, Y.; WANG, W. B.; GUO, P.; WU, K. G.; PENG, G.-N. Epidemiology of Cryptosporidium infection in cattle in China: a review. Parasite, Les Ulis, v. 24, n. 1, p. 1-8, 2017.
KVÁČ, M.; HAVRDOVÁ, N.; HLÁSKOVÁ, L.; DAŇKOVÁ, T.; KANDĚRA, J.; JEŽKOVÁ, J.; VÍTOVEC, J.; SAK, B.; ORTEGA, Y.; XIAO, L.; MODRÝ, D.; CHELLADURAI; J. R.; PRANTLOVÁ, V.; MCEVOY, J. Cryptosporidium proliferans n. sp. (Apicomplexa: Cryptosporidiidae): Molecular and biological evidence of cryptic species within gastric Cryptosporidium of mammals. Plos One, San Francisco, v. 11, n. 1, p. 1-24, 2016.
LEARMONTH, J. J.; IONAS, G.; EBBETT, K. A.; KWAN, E. S. Genetic characterization and transmission cycles of Cryptosporidium species isolated from humans in New Zealand. Applied and Environmental Microbiology, Washington DC, v. 70, n. 7, p. 3973-3978, 2004.
MAKATU, M. Y; SOARES, J. A.; MATOS, L. V. S.; COELHO, W. M. D.; MANHOSO, F. F. R.; KANETO, C. N.; BRESCIANI, K. D. S. Perfil dos produtores rurais de dois assentamentos nos municípios de Brejo Alegre e Birigui, Estado de São Paulo. Veterinária e Zootecnia, Botucatu, v. 24, n. 2, 373-383, 2017.
NAKAMURA, A. A.; MEIRELES, M. V. Cryptosporidium infections in birds – a review. Brazilian Journal of Veterinary Parasitology, Jaboticabal, v. 24, n. 3, p. 253-267, 2015.
OUAKLI, N.; BELKHIRI, A.; LUCIO, A.; KÖSTER, P. C.; DJOUDI, M.; DADDA, A.; KHELEF, D.; KAIDI, R.; CARMENA, D. Cryptosporidium-associated diarrhoea in neonatal calves in Algeria. Veterinary Parasitology: Regional Studies and Reports, Geneva, v. 12, p. 78-84, 2018.
PAZ E SILVA, F. M.; LOPES, R. S.; ARAUJO, J. R.; HIGH, J. P. Identification of Cryptosporidium species and genotypes in dairy cattle in Brazil. Brazilian Journal of Veterinary Parasitology, Jaboticabal, v. 22, n. 1, p. 22-28, 2013.
SANTÍN, M.; TROUT, J. M. Cryptosporidiosis of livestock. In: FAYER, R., XIAO, L. (Ed.). Cryptosporidium and Cryptosporidiosis. Boca Raton: FL CRC Press, 2008. p. 451-483, v. 2.
SANTÍN, M.; TROUT, J. B. M.; XIAO, L.; ZHOU, L.; GREINER, E.; FAYER, R. Prevalence and age-related variation of Cryptosporidium species and genotypes in dairy calves. Veterinary Parasitology, Amsterdam, v. 122, n. 2, p. 103-117, 2004.
SOPWITH, W.; OSBORN, K.; CHALMERS, R.; REGAN, M. The changing epidemiology of cryptosporidiosis in North West England. Epidemiology and Infection, Cambridge, v. 133, n. 5, p. 785-793, 2005.
THOMPSON, J. D.; GIBSON, T. J.; PLEWNIAK, F.; JEANMOUGIN, F.; HIGGINS, D. G. The CLUSTALX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, Oxford, v. 25, n. 24, p. 4876-4882, 1997.
WANG, L.; XUE, X.; LI, J.; ZHOU, Q.; YU, Y.; DU, A. Cryptosporidiosis in broiler chickens in Zhejiang Province, China: molecular characterization of oocysts detected in fecal samples. Parasite, Les Ulis, v. 21, n. 36, p. 1-5, 2014.
WEGAYEHU T.; KARIM, R.; ANBERBER, M.; ADAMU, H.; ERKO, B.; ZHANG, L.; TILAHUN, G. Prevalence and genetic characterization of Cryptosporidium species in dairy calves in Central Ethiopia. Plos One, San Francisco, v. 11, n. 5, p. 1-11, 2016.
XIAO, L. Molecular epidemiology of cryptosporidiosis: an update. Experimental Parasitology, v. 124, n. 1, p. 80-89, 2010.
XIAO, L.; LIMOR, J.; MORGAN, U. M.; SULAIMAN, I. M.; THOMPSON, R. C. A.; LAL, A. A. Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites. Applied and Environmental Microbiology, Washington DC, v. 66, n. 12, p. 5499-5502, 2000.
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Published
2019-02-15
How to Cite
Matos, L. V. S. de, Silveira Neto, L. da, Oliveira, B. C. M., Makatu, M. Y., Pierucci, J. C., Viol, M. A., … Bresciani, K. D. S. (2019). Molecular characterization of Cryptosporidium in calves from rural settlements in the Northwest region of the state of São Paulo, Brazil. Semina: Ciências Agrárias, 40(1), 491–496. https://doi.org/10.5433/1679-0359.2019v40n1p491
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