Anti-equine arteritis virus activity of ethanolic extract and compounds from Origanum vulgare

Authors

  • Daiane Einhardt Blank Universidade Federal de Pelotas
  • Rayra Almeida Corrêa Universidade Federal do Rio Grande do Sul
  • Rogério Antônio Freitag Universidade Federal de Pelotas
  • Marlete Brum Cleff Universidade Federal de Pelotas
  • Silvia de Oliveira Hübner Universidade Federal de Pelotas

DOI:

https://doi.org/10.5433/1679-0359.2017v38n2p759

Keywords:

Antimicrobial, Antiviral, Cytotoxicity, Origanum sp, Virucidal.

Abstract

The equine arteritis virus (EAV) is responsible by an important respiratory and reproductive disease in equine populations and there is no specific antiviral treatment available. The objective of this study was to investigate the activity of an ethanolic crude extract of Origanum vulgare (EEO) and of isolated compound caffeic acid, p-coumaric acid, rosmarinic acid, quercetin, luteolin, carnosol, carnosic acid, kaempferol and apigenin against EAV. The assays were performed using non-cytotoxic concentrations. The antiviral activity was monitored initially by cytopathic effect inhibition (CPE) assay in RK13 cells in the presence or absence of EEO. Pre-incubated cells with EEO were also examined to show prophylactic effect. Direct viral inactivation by EEO and isolated compounds was evaluated by incubation at 37°C or 20°C. After the incubation period, the infectivity was immediately determined by virus titrations on cell cultures and expressed as 50% tissue culture infective dose (TCID50)/100 µL. There was significant virucidal activity of EEO and of the compounds caffeic acid, p-coumaric acid, quercetin, carnosic acid and kaempferol. When EEO was added after infection, EEO inhibited the virus growth in infected cells, as evidenced by significant reduction of the viral titre. The results provide evidence that the EEO exhibit an inhibitory effect anti-EAV. Among the main compounds evaluated, caffeic acid, p-coumaric acid, carnosic acid, kaempferol and mainly quercetin, contributed to the activity of EEO. EEO may represent a good prototype for the development of a new antiviral agent, presenting promising for combating arteriviruses infections.

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Author Biographies

Daiane Einhardt Blank, Universidade Federal de Pelotas

Discente, Programa de Pós-Graduação em Bioquímica e Bioprospecção, Universidade Federal de Pelotas, UFPel, RS, Brasil.

Rayra Almeida Corrêa, Universidade Federal do Rio Grande do Sul

Discente, Programa de Pós-Graduação em Ciências Veterinárias, Universidade Federal do Rio Grande do Sul, UFRGS, RS, Brasil.

Rogério Antônio Freitag, Universidade Federal de Pelotas

Prof., Departamento de Química Orgânica, UFPel, RS, Brasil.

Marlete Brum Cleff, Universidade Federal de Pelotas

Prof., Departamento de Clínica Veterinária, UFPel, RS, Brasil.

Silvia de Oliveira Hübner, Universidade Federal de Pelotas

Profª, Departamento de Veterinária Preventiva, UFPel, RS, Brasil.

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Published

2017-05-02

How to Cite

Blank, D. E., Corrêa, R. A., Freitag, R. A., Cleff, M. B., & Hübner, S. de O. (2017). Anti-equine arteritis virus activity of ethanolic extract and compounds from Origanum vulgare. Semina: Ciências Agrárias, 38(2), 759–764. https://doi.org/10.5433/1679-0359.2017v38n2p759

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