Detection of larvae of Toxocara canis in milk: an experimental study in rabbits

Autores

  • Vamilton Alvares Santarém Universidade do Oeste Paulista
  • Célia Fátima Silva Exposto Universidade do Oeste Paulista
  • Lívia Magosso Ramires Universidade do Oeste Paulista
  • Lundia Luara Cavalcante Bin Universidade do Oeste Paulista
  • Guita Rubinsky-Elefant Instituto de Medicina Tropical de São Paulo
  • Cecília Braga Laposy Universidade do Oeste Paulista
  • Rosa Maria Barilli Nogueira Universidade do Oeste Paulista

DOI:

https://doi.org/10.5433/1679-0359.2014v35n1p357

Palavras-chave:

Toxocariasis, Lactation, Diagnosis.

Resumo

Toxocariasis, caused most commonly by Toxocara canis, is an important cosmopolitan zoonosis. Paratenic hosts have been employed to provide knowledge regard to the transmission of toxocariasis. Transmammary transmission in murine experimentally infected was observed based on the recovery of larvae from the tissue. The aim of this study was to evaluate the possibility of transmammary transmission of Toxocara canis in rabbits by detecting larvae directly in milk. Seventeen sexually mature virgin white New Zealand female rabbits were divided into two groups. Twelve animals were orally inoculated with 1,000 T. canis embryonated eggs (infected group), and five animals remained uninfected (control group). One month following the infection, the females were mated. Manual collection of 500 ?L of milk from each rabbit was performed on days +7, +14 and +21 of lactation for three consecutive lactations. The recovery of larvae was determined via a centrifuge-sedimentation technique using ether and formalin solutions. ELISA test was run to confirm the production of anti-T. canis antibodies (IgG) by infected rabbits. The presence of larvae was observed in milk samples from 5 (41.7%) of the 12 infected rabbits. The total number of recovered larvae was 20, ranging from 1 to 4 larvae per lactation/rabbit. Larvae were recovered exclusively on days 7 and 14 of lactation. Recovery was verified in different lactations. No significant difference was observed with respect to the number of larvae either in the same lactation period or in different lactation periods. Anti-T. canis antibodies were detected in all infected rabbits. In conclusion, the presence of larvae in rabbit milk samples suggests the possibility of galactogenic transmission of T. canis in paratenic hosts. Moreover, the technique employed in this study allows for the recovery of larvae directly from milk.

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Biografia do Autor

Vamilton Alvares Santarém, Universidade do Oeste Paulista

Médico Veterinário, Prof. de Mestrado em Ciência Animal, Hospital Veterinário, Universidade do Oeste Paulista, UNOESTE, Presidente Prudente, São Paulo, SP, Brasil.

Célia Fátima Silva Exposto, Universidade do Oeste Paulista

Médica Veterinária, Discente de Mestrado em Ciência Animal UNOESTE, São Paulo, SP, Brasil.

Lívia Magosso Ramires, Universidade do Oeste Paulista

Médica Veterinária, Discente de Mestrado em Ciência Animal UNOESTE, São Paulo, SP, Brasil.

Lundia Luara Cavalcante Bin, Universidade do Oeste Paulista

Médica Veterinária, Discente de Mestrado em Ciência Animal UNOESTE, São Paulo, SP, Brasil.

Guita Rubinsky-Elefant, Instituto de Medicina Tropical de São Paulo

Farmacêutica Bioquímica, Laboratório de Seroepidemiologia e Imunobiologia. Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, USP, São Paulo, SP, Brasil.

Cecília Braga Laposy, Universidade do Oeste Paulista

Médica Veterinária, Profª de Mestrado em Ciência Animal, Hospital Veterinário, Universidade do Oeste Paulista, UNOESTE, Presidente Prudente, São Paulo, SP, Brasil.

Rosa Maria Barilli Nogueira, Universidade do Oeste Paulista

Médica Veterinária, Profª de Mestrado em Ciência Animal, Hospital Veterinário, Universidade do Oeste Paulista, UNOESTE, Presidente Prudente, São Paulo, SP, Brasil.

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Publicado

2014-02-27

Como Citar

Santarém, V. A., Exposto, C. F. S., Ramires, L. M., Bin, L. L. C., Rubinsky-Elefant, G., Laposy, C. B., & Nogueira, R. M. B. (2014). Detection of larvae of Toxocara canis in milk: an experimental study in rabbits. Semina: Ciências Agrárias, 35(1), 357–364. https://doi.org/10.5433/1679-0359.2014v35n1p357

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